Monocyte thromboplastin (tissue factor): complementary effect of lymphocytes upon its generation by endotoxin-stimulated monkey (Macaca fuscata) cells.

abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

journal_title：Journal of biochemistry

authors： Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

更新日期：1980-03-01 00:00:00

abstract：:Cytochrome c oxidase from Thiobacillus ferrooxidans was purified to homogeneity and some of its properties were studied. The oxidase was solubilized with n-octyl-beta-D-thioglucoside (OTG) under acidic conditions (pH 4.0) and purified by one step of ion-exchange chromatography with a CM-Toyopearl column. The absorptio...

journal_title：Journal of biochemistry

authors： Kai M,Yano T,Tamegai H,Fukumori Y,Yamanaka T

更新日期：1992-12-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:To gain insight into the mechanism by which the sequence at the rne-dependent site of substrate RNA affects the substrate specificity of Escherichia coli RNase E, we performed kinetic analysis of the cleavage of precursor M1 RNA molecules containing various sequences at the rne-dependent site by the N-terminal catalyt...

journal_title：Journal of biochemistry

authors： Kim KS,Sim S,Ko JH,Cho B,Lee Y

更新日期：2004-11-01 00:00:00

abstract：:Aspartic proteases of human malarial parasites are thought to play key roles in essential pathways of merozoite release, invasion and host cell hemoglobin degradation during the intraerythrocytic stages of their life cycle. Therefore, we have purified and characterized Plasmodium vivax aspartic protease, to determine ...

journal_title：Journal of biochemistry

authors： Sharma A,Eapen A,Subbarao SK

更新日期：2005-07-01 00:00:00

abstract：:DNA synthesis in the Bacillus subtilis cells has become possible using extra-cellular DNA. Generally, purified DNAs in a test tube have been required to introduce into the host cells for molecular cloning technology in the laboratory. We have developed a cell lysis technique for natural transformation using stable ext...

journal_title：Journal of biochemistry

authors： Kaneko S,Fukushima H,Nakahama M,Asano S,Miyazaki Y,Aizawa Y,Itaya M

更新日期：2018-04-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

journal_title：Journal of biochemistry

authors： Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

更新日期：1996-08-01 00:00:00

abstract：:Two G-protein-coupled receptors (GPCRs) that couple with Gαq/11, B2 bradykinin (BK) receptor (B2R) and ATP/UTP receptor P2Y2 (P2Y2R), are ubiquitously expressed and responsible for vascular tone, inflammation, and pain. We analysed the cellular signalling of P2Y2Rs in cells that express B2Rs. B2R desensitization induc...

journal_title：Journal of biochemistry

authors： Yashima S,Shimazaki A,Mitoma J,Nakagawa T,Abe M,Yamada H,Higashi H

更新日期：2015-08-01 00:00:00

abstract：:An enzyme activity specifically active on nitrous acid-treated DNA was found in an extract of Escherichia coli. The enzyme acts on both double- and single-stranded DNAs, treated with nitrous acid, in the presence of EDTA, although the former DNA is a better substrate. Evidence is presented that nitrous acid- and bisul...

journal_title：Journal of biochemistry

authors： Oeda K,Shimizu K,Sekiguchi M

更新日期：1978-11-01 00:00:00

abstract：:The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

journal_title：Journal of biochemistry

authors： Nakajima Y,Nakamura K

更新日期：1991-02-01 00:00:00

abstract：:The juvenile visceral steatosis (JVS) mouse is a novel mutant animal for studying systemic carnitine deficiency. The importance of the model has been pointed out in carnitine-deficient cardiac hypertrophy, since cardiomyopathy has been often improved after oral carnitine therapy in human systemic carnitine deficiency....

journal_title：Journal of biochemistry

authors： Uenaka R,Kuwajima M,Ono A,Matsuzawa Y,Hayakawa J,Inohara N,Kagawa Y,Ohta S

更新日期：1996-03-01 00:00:00

abstract：:Guanosine 3',5'-monophosphate (cyclic GMP)-dependent protein kinase purified from silkworm pupae reacts with rat liver ribosomal proteins when a stimulatory modulator (Kuo, W.N. & Kuo, J.F. 1976) J. Biol. Chem. 251, 4283-4286) is added to the reaction mixture. Judging from autoradiogram of the radioactive proteins sep...

journal_title：Journal of biochemistry

authors： Chihara-Nakashima M,Hashimoto E,Nishizuka Y

更新日期：1977-06-01 00:00:00

abstract：:We studied the regulation of lipid body biogenesis in the oleaginous fungus Mortierella ramanniana var. angulispora by investigating culture conditions to modulate lipid body size, which we found was affected by the carbon-to-nitrogen ratio (C/N ratio) in the culture medium. Increasing the nitrogen source or decreasin...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Noda N,Yamaoka M

更新日期：2004-02-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00

abstract：:Pyridylamino sugar chains were converted to the corresponding reducing sugar chains by first converting them to 1-amino-1-deoxy derivatives using the method previously reported [S. Hase, J. Biochem. 112, 266-268 (1992)] and then converting the products to the corresponding reducing sugar chains using the Sommlet react...

journal_title：Journal of biochemistry

authors： Takahashi C,Nakakita S,Hase S

更新日期：2003-07-01 00:00:00

abstract：:The effects of N,N'-dicyclohexylcarbodiimide (DCCD) on light-induced H+-transport and transmembrane electric potential (delta phi) formation were studied in the membrane vesicles of Halobacterium halobium R1M1. In accordance with our previous finding of the existence of two DCCD-binding components in vesicle membrane ...

journal_title：Journal of biochemistry

authors： Murakami N,Konishi T

更新日期：1985-10-01 00:00:00

abstract：:The crystal structures of the GTP- and GDP-bound alpha subunits of heterotrimeric GTP-binding proteins were recently determined, and a conserved Thr residue in the G2 (linker 2) region of the alpha subunits, which corresponds to Thr182 in Gi2 alpha, was deduced to interact with the gamma-phosphate of GTP and Mg2+. To ...

journal_title：Journal of biochemistry

authors： Nishina H,Nimota K,Kukimoto I,Maehama T,Takahashi K,Hoshino S,Kanaho Y,Katada T

更新日期：1995-11-01 00:00:00

abstract：:Laccase is an enzyme that catalyzes the oxidation of phenolic compounds by coupling the reduction of oxygen to water. While many laccases have been identified in plant and fungal species, enzymes of prokaryotic origin are poorly known. Here we report the enzymological characterization of EpoA, a laccase-like extracyto...

journal_title：Journal of biochemistry

authors： Endo K,Hayashi Y,Hibi T,Hosono K,Beppu T,Ueda K

更新日期：2003-05-01 00:00:00

abstract：:Troponin-tropomyosin complex from skeletal muscles was observed to regulate sliding movement of actin filaments on myosin molecules in a manner independent of their ATPase activity. When actin molecules were crosslinked with DSS (disuccinimidyl suberate), the myosin ATPase activity in the presence of the modified acti...

journal_title：Journal of biochemistry

authors： Honda H,Tagami N,Hatori K,Matsuno K

更新日期：1997-01-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:The gene of an extreme thermophile, Thermus thermophilus HB8, which codes for a leucine biosynthetic enzyme, 3-isopropylmalate (3-IPM) dehydrogenase [EC 1.1.1.85], was cloned in Escherichia coli using pBR322 as a vector. E. coli cells carrying this recombinant plasmid, pHB2, produced the thermophilic enzyme 7-fold mor...

journal_title：Journal of biochemistry

authors： Tanaka T,Kawano N,Oshima T

更新日期：1981-02-01 00:00:00

abstract：:The effect of concentration of rabbit reticulocyte rRNAs on the translation of rabbit globin mRNAs was studied by using a rabbit reticulocyte lysate system. Globin synthesis was studied using L-[U-14C]leucine. Both the 18S and 28S rRNA inhibited globin synthesis. The 18S rRNA inhibited the synthesis of alpha-globin ch...

journal_title：Journal of biochemistry

authors： Suzuki H

更新日期：1978-04-01 00:00:00

abstract：:Wild mice are divided into two groups with different ganglioside compositions in the liver. Most Japanese and a few Chinese wild mice have GM2(NeuGc) as a major ganglioside, whereas all wild mice caught at other places distributed all over the world other than Japan and China express GM1(NeuGc) and GD1a(NeuGc) in addi...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki A,Yamakawa T,Wang CH,Bonhomme F,Miyashita N,Moriwaki K

更新日期：1984-01-01 00:00:00

abstract：:Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...

journal_title：Journal of biochemistry

authors： Teshima K,Ikeda K,Hamaguchi K,Hayashi K

更新日期：1983-07-01 00:00:00

abstract：:Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

journal_title：Journal of biochemistry

authors： Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

更新日期：1977-07-01 00:00:00

abstract：:Four motor proteins, MotX, MotY, PomA, and PomB, have been identified as constituents of the Na(+)-driven flagellum of Vibrio species. In this study, the complete motX gene was cloned from Vibrio alginolyticus and shown to complement three mot mutations, motX94, motX115, and motX119, as well as a V. parahaemolyticus m...

journal_title：Journal of biochemistry

authors： Okabe M,Yakushi T,Asai Y,Homma M

更新日期：2001-12-01 00:00:00

abstract：:Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2-5A synthetases) were estimated. The 2-5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20...

journal_title：Journal of biochemistry

authors： Shimizu N,Sokawa Y,Sokawa J

更新日期：1984-06-01 00:00:00

abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...

journal_title：Journal of biochemistry

authors： Yohda M,Yoshida M

更新日期：1987-10-01 00:00:00

abstract：:A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

journal_title：Journal of biochemistry

authors： Miyazawa K,Mori A,Okudaira H

更新日期：1998-12-01 00:00:00