Enzymological characterization of EpoA, a laccase-like phenol oxidase produced by Streptomyces griseus.


:Laccase is an enzyme that catalyzes the oxidation of phenolic compounds by coupling the reduction of oxygen to water. While many laccases have been identified in plant and fungal species, enzymes of prokaryotic origin are poorly known. Here we report the enzymological characterization of EpoA, a laccase-like extracytoplasmic phenol oxidase produced by Streptomyces griseus. EpoA was expressed and purified with an Escherichia coli host-vector system as a recombinant protein fused with a C-terminal histidine-tag (rEpoA). Physicochemical analyses showed that rEpoA comprises a stable homotrimer containing all three types of copper (types 1-3). Various known laccase substrates were oxidized by rEpoA, while neither syringaldazine nor guaiacol served as substrates. Among the substrates examined, rEpoA most effectively oxidized N,N-dimethyl-p-phenylenediamine sulphate with a Km value of 0.42 mM. Several metal chelators caused marked inhibition of rEpoA activity, implying the presence of a metal center essential for the oxidase activity. The pH and temperature optima of rEpoA were 6.5 and 40 degrees C, respectively. The enzyme retained 40% activity after preincubation at 70 degrees C for 60 min. EpoA-like activities were detected in cell extracts of 8/40 environmental actinomycetes strains, which suggests that similar oxidases are widely distributed among this group of bacteria.


J Biochem


Journal of biochemistry


Endo K,Hayashi Y,Hibi T,Hosono K,Beppu T,Ueda K





Has Abstract


2003-05-01 00:00:00












  • Microtubule elongation along actin filaments induced by microtubule-associated protein 4 contributes to the formation of cellular protrusions.

    abstract::Actin-microtubule crosstalk is implicated in the formation of cellular protrusions, but the mechanism remains unclear. In this study, we examined the regulation of cell protrusion involving a ubiquitously expressed microtubule-associated protein (MAP) 4, and its superfamily proteins, neuronal MAP2 and tau. Fluorescenc...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Doki C,Nishida K,Saito S,Shiga M,Ogara H,Kuramoto A,Kuragano M,Nozumi M,Igarashi M,Nakagawa H,Kotani S,Tokuraku K

    更新日期:2020-09-01 00:00:00

  • Affinity labeling of the myosin ATPase with ribose-modified fluorescent nucleotides and vanadate.

    abstract::Ribose-modified fluorescent nucleotide analogs, 3'-O-anthraniloyl and 3'-O-(N-methylanthraniloyl) derivatives of AT(D)P, dAT(D)P, CT(D)P, UT(D)P, IT(D)P, and GT(D)P, were synthesized for use as substrates and affinity labels for the myosin ATPase [Hiratsuka, T. (1983) Biochim. Biophys. Acta 742, 496-508]. None of the ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hiratsuka T

    更新日期:1984-07-01 00:00:00

  • The effects of mutations at position 253 on the thermostability of the Bacillus subtilis 3-isopropylmalate dehydrogenase subunit interface.

    abstract::3-Isopropylmalate dehydrogenase (IPMDH) is a dimeric enzyme with a strongly hydrophobic core that is composed of residues from four alpha-helices. We replaced Glu253, which is found in the hydrophobic core and is part of the subunit interface of the Bacillus subtilis (Bs) IPMDH, with several other amino acids to probe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohkuri T,Yamagishi A

    更新日期:2007-06-01 00:00:00

  • Induction of enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in media supplemented with fatty acid.

    abstract::Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sato S,Imamura S,Ozeki Y,Kawaguchi A

    更新日期:1992-01-01 00:00:00

  • A screening system for artificial small RNAs that inhibit the growth of Escherichia coli.

    abstract::We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

    更新日期:2011-09-01 00:00:00

  • An isoform of microtubule-associated protein 4 inhibits kinesin-driven microtubule gliding.

    abstract::Recently, we revealed that microtubule-associated protein (MAP) 4 isoforms, which differ in the number of repeat sequences, alter the microtubule surface properties, and we proposed a hypothesis stating that the change in the surface properties may regulate the movements of microtubule motors [Tokuraku et al. (2003) J...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tokuraku K,Noguchi TQ,Nishie M,Matsushima K,Kotani S

    更新日期:2007-04-01 00:00:00

  • The characterization of 2,3-alkanediol diacyl esters obtained from the Harderian glands of Mongolian gerbil (Meriones unguiculatus).

    abstract::A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

    更新日期:1983-12-01 00:00:00

  • Retinoic acid-specific induction of a protein kinase C isoform during differentiation of HL-60 cells.

    abstract::Human promyelocytic leukemia cells (HL-60) were treated with several differentiation inducers, then the changes in the activity of cytosolic protein kinase C (PKC) isoforms were examined by hydroxylapatite chromatography and the species of the isoforms were determined immunologically. In three undifferentiated HL-60 c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tanaka Y,Yoshihara K,Tsuyuki M,Itaya-Hironaka A,Inada Y,Kamiya T

    更新日期:1992-02-01 00:00:00

  • Interaction between D-amino acid oxidase and small molecules.

    abstract::1. From the standpoint of monomer-dimer equilibrium of hog kidney D-amino acid oxidase [EC] and the interaction between the enzyme and small molecules, the effect of pH on the binding of p-aminobenzoate to the monomer and dimer of the enzyme was studied by kinetic methods and spectrophotometric titration. 2. T...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Horiike K,Shiga K,Isomoto A,Yamano T

    更新日期:1976-11-01 00:00:00

  • Blood group A activities of glycoprotein and glycolipid from human erythrocyte membranes.

    abstract::It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamato K,Handa S,Yamakawa T

    更新日期:1975-12-01 00:00:00

  • Aspartate aminotransferase from an alkalophilic Bacillus contains an additional 20-amino acid extension at its functionally important N-terminus.

    abstract::Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

    更新日期:1996-08-01 00:00:00

  • The role of nuclear serine proteases in the degradation of newly synthesized histones and ribosomal proteins.

    abstract::To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsurugi K,Oyanagi M,Ogata K

    更新日期:1983-05-01 00:00:00

  • Functional role of glycosphingolipids in cell recognition and signaling.

    abstract::Glycosphingolipids (GSLs), cell type-specific markers which change dramatically during ontogenesis and oncogenesis, have been implicated as playing major roles in cellular interactions and control of cell proliferation in multicellular organisms. These functional roles have been partially clarified through two types o...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Hakomori S,Igarashi Y

    更新日期:1995-12-01 00:00:00

  • Immunological identification of the major disulfide-linked light component of silk fibroin.

    abstract::The light protein components of silk fibroin were analyzed by Western blotting using polyclonal antibodies against two synthetic peptides that are segment of the light (L-) chain [Yamaguchi et al. (1989) J. Mol. Biol. 210, 127-139] and P25 [Chevillard et al. (1986) Sericologia 26, 435-449], respectively, and against t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tanaka K,Mori K,Mizuno S

    更新日期:1993-07-01 00:00:00

  • Effects of random mutagenesis in a putative substrate-binding domain of geranylgeranyl diphosphate synthase upon intermediate formation and substrate specificity.

    abstract::Archaeal geranylgeranyl diphosphate (GGPP) synthase catalyzes the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce GGPP with significant amounts of intermediates. To obtain information about the amino acids involved in the condensation and the release of intermediates, we ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohnuma S,Hemmi H,Ohto C,Nakane H,Nishino T

    更新日期:1997-04-01 00:00:00

  • Development of a novel preparation method of recombinant proteoliposomes using baculovirus gene expression systems.

    abstract::We have developed a novel method for the preparation of 'recombinant proteoliposomes'. Membrane proteins were expressed on budded virus (BV) envelopes using baculovirus gene expression systems, and proteoliposomes were prepared by fusion of these viruses with liposomes. First, plasmid DNA containing the gene for the t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fukushima H,Mizutani M,Imamura K,Morino K,Kobayashi J,Okumura K,Tsumoto K,Yoshimura T

    更新日期:2008-12-01 00:00:00

  • Inhibition of globin synthesis by rabbit reticulocyte rRNAs.

    abstract::The effect of concentration of rabbit reticulocyte rRNAs on the translation of rabbit globin mRNAs was studied by using a rabbit reticulocyte lysate system. Globin synthesis was studied using L-[U-14C]leucine. Both the 18S and 28S rRNA inhibited globin synthesis. The 18S rRNA inhibited the synthesis of alpha-globin ch...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suzuki H

    更新日期:1978-04-01 00:00:00

  • Protein phosphatase 2A inhibitors, phoslactomycins. Effects on the cytoskeleton in NIH/3T3 cells.

    abstract::Protein phosphorylation is a key regulatory mechanism of the organization and dynamics of the actin cytoskeleton during cell motility, differentiation, and cytokinesis. The level of protein phosphorylation is dependent on the relative activities of both protein kinases and protein phosphatases. In this paper, we exami...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Usui T,Marriott G,Inagaki M,Swarup G,Osada H

    更新日期:1999-05-01 00:00:00

  • Determination of the DNA-binding sequences of the Zn(II)2Cys6 zinc-cluster-containing PRIB protein, derived from the basidiomycete Lentinus edodes gene.

    abstract::The 565 amino-acid PRIB protein with a Zn(II)2Cys6 zinc-cluster DNA-binding motif is the expression product of the priB gene, which is most actively transcribed in an early stage of fruiting-body formation by the basidiomycete, Lentinus edodes. PRIB produced in Escherichia coli using the bacteriophage T7 expression sy...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Miyazaki Y,Tsunoka O,Shishido K

    更新日期:1997-12-01 00:00:00

  • Cloning of 3-isopropylmalate dehydrogenase gene of an extreme thermophile and partial purification of the gene product.

    abstract::The gene of an extreme thermophile, Thermus thermophilus HB8, which codes for a leucine biosynthetic enzyme, 3-isopropylmalate (3-IPM) dehydrogenase [EC], was cloned in Escherichia coli using pBR322 as a vector. E. coli cells carrying this recombinant plasmid, pHB2, produced the thermophilic enzyme 7-fold mor...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tanaka T,Kawano N,Oshima T

    更新日期:1981-02-01 00:00:00

  • Human erythrocyte bisphosphoglycerate mutase: inactivation by glycation in vivo and in vitro.

    abstract::2,3-Bisphosphoglycerate mutase (BPGM) [EC] is a multifunctional enzyme that catalyzes both the synthesis and the degradation of 2,3-diphosphoglycerate (2,3-DPG) and contains three types of activities in that it functions as a 2,3-DPG synthetase, a phosphoglycerate mutase and a 2,3-DPG phosphatase. In humans, B...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujita T,Suzuki K,Tada T,Yoshihara Y,Hamaoka R,Uchida K,Matuo Y,Sasaki T,Hanafusa T,Taniguchi N

    更新日期:1998-12-01 00:00:00

  • Circular dichroism of bacteriochlorophyll a in light harvesting bacteriochlorophyll protein complexes from Chromatium vinosum.

    abstract::Bacteriochlorophyll (Bchl) protein complexes containing light harvesting (LH) Bchls were isolated from Chromatium vinosum, and their CD spectra were measured in the near-infrared region. These isolated Bchl protein complexes retained the CD signals of LH Bchls that were observed in situ in chromatophores. The CD spect...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hayashi H,Nozawa T,Hatano M,Morita S

    更新日期:1981-06-01 00:00:00

  • Mechanism-based inactivation of coenzyme B12-dependent diol dehydratase by 3-unsaturated 1,2-diols and thioglycerol.

    abstract::The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

    更新日期:2008-10-01 00:00:00

  • Bacillus thermoglucosidius alpha-glucosidase. Temperature dependence of activity and stability.

    abstract::A p-nitrophenyl-alpha-D-glucopyranoside-hydrolyzing alpha-glucosidase from an obligate thermophile, Bacillus thermoglucosidius KP 1006, gave a triphasic relationship at pH 6.8 in the van't Hoff plot of Km, in the Arrhenius plot of the first order rate constant of inactivation with 0.1% sodium dodecyl sulfate, and in t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suzuki Y,Nakamura N,Kishigami T,Abe S

    更新日期:1980-03-01 00:00:00

  • Characterization and amino acid sequence of a new acidic cysteine proteinase inhibitor (cystatin SA) structurally closely related to cystatin S, from human whole saliva.

    abstract::A cysteine proteinase inhibitor (designated as cystatin SA) was isolated from human whole saliva by procedures including chromatography on DE 32 and DEAE-Sepharose CL-6B. The amino acid sequence determined by conventional methods showed sequence homology of 90 and 87% as compared with the sequences of cystatin S and c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Isemura S,Saitoh E,Sanada K

    更新日期:1987-10-01 00:00:00

  • A cell-free system from ethionine-treated rat liver active in initiation of protein synthesis.

    abstract::A cell-free protein-synthesizing system active in initiation of translation of both endogenous mRNA and exogenous mRNA has been obtained from postmitochondrial supernatant (S-12) of the liver of ethionine-treated rats by adding reticulocyte ribosomal extract as a source of initiation factor. Formation of polysomes in ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hase M,Endo Y,Natori Y

    更新日期:1982-05-01 00:00:00

  • Characterization of porcine adrenocortical lysosomes.

    abstract::Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

    更新日期:1981-05-01 00:00:00

  • Effect of dietary protein content on the activity of rat liver asparagine synthetase.

    abstract::The activity of rat liver asparagine synthetase [EC]increased when animals maintained on 25% protein diet were placed on 15% or 6% protein diet. The enzyme activity level rose within one day, reached a maximum in 7 or 10 days after switching the diet and thereafter dropped gradually. During the purification of...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hongo S,Matsumoto T,Suzuki I,Sato T

    更新日期:1979-08-01 00:00:00

  • The Yin and Yang of tumor-derived extracellular vesicles in tumor immunity.

    abstract::Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamauchi T,Moroishi T

    更新日期:2020-11-23 00:00:00

  • 3(20)alpha-hydroxysteroid dehydrogenase activity of monkey liver indanol dehydrogenase.

    abstract::Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hara A,Nakagawa M,Taniguchi H,Sawada H

    更新日期:1989-11-01 00:00:00