Abstract:
:The planar cell polarity (PCP) protein, Prickle (Pk), is conserved in invertebrates and vertebrates, and regulates cellular morphogenesis and movement. Vertebrate Pk consists of at least two family members, Pk1 and Pk2, both of which are expressed in the brain; however, their localization and function at synapses remain elusive. Here, we show that Pk2 is expressed mainly in the adult brain and is tightly associated with the postsynaptic density (PSD) fraction obtained by subcellular fractionation. In primary cultured rat hippocampal neurons, Pk2 is colocalized with PSD-95 and synaptophysin at synapses. Moreover, immunoelectron microcopy shows that Pk2 is localized at the PSD of asymmetric synapses in the hippocampal CA1 region. Biochemical assays identified that Pk2 forms a complex with PSD proteins including PSD-95 and NMDA receptor subunits via the direct binding to the C-terminal guanylate kinase domain of PSD-95. These results indicate that Pk2 is a novel PSD protein that interacts with PSD-95 and NMDA receptors through complex formations in the brain.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Hida Y,Fukaya M,Hagiwara A,Deguchi-Tawarada M,Yoshioka T,Kitajima I,Inoue E,Watanabe M,Ohtsuka Tdoi
10.1093/jb/mvr023subject
Has Abstractpub_date
2011-06-01 00:00:00pages
693-700issue
6eissn
0021-924Xissn
1756-2651pii
mvr023journal_volume
149pub_type
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