Specificity of hydrogen transfer of mammalian and avian carbonyl and aldehyde reductases.

abstract：:Studies were conducted to induce urinary excretion of retinol-binding protein (RBP) in the rabbit by long-term administration of cadmium, and to isolate and characterize rabbit RBP. Two rabbits were exposed to Cd at a dose of 0.8 and/or 1.5mg/kg of body weight by means of subcutaneous injections five times per week. O...

journal_title：Journal of biochemistry

authors： Muto Y,Nakanishi M,Shidoji Y

更新日期：1976-04-01 00:00:00

abstract：:The interaction between pullulanase from Klebsiella pneumoniae and alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was examined by means of inhibition studies of the enzyme activity, UV difference spectroscopy, and flow calorimetry. All the abov...

journal_title：Journal of biochemistry

authors： Iwamoto H,Ohmori M,Ohno M,Hirose J,Hiromi K,Fukada H,Takahashi K,Hashimoto H,Sakai S

更新日期：1993-01-01 00:00:00

abstract：:The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

journal_title：Journal of biochemistry

authors： Giga Y,Ikai A

更新日期：1985-07-01 00:00:00

abstract：:Members of the transforming growth factor-β (TGF-β) family, including TGF-βs, activin and bone morphogenetic proteins (BMPs), are multifunctional proteins that regulate a wide variety of cellular responses, such as proliferation, differentiation, migration and apoptosis. TGF-β family signalling is mainly mediated by m...

journal_title：Journal of biochemistry

authors： Imamura T,Oshima Y,Hikita A

更新日期：2013-12-01 00:00:00

abstract：:The pH dependence of the binding constant of Ca2+ to a phospholipase A2 of Agkistrodon halys blomhoffii, in which the alpha-amino group had been selectively modified to an alpha-keto group, was studied at 25 degrees C and ionic strength 0.1 by the tryptophyl fluorescence method. The dependence was compared with the re...

journal_title：Journal of biochemistry

authors： Haruki H,Teshima K,Samejima Y,Kawauchi S,Ikeda K

更新日期：1986-01-01 00:00:00

abstract：:To analyze the processing of extracellular enzymes of Bacillus subtilis, an NH2-terminally extended hybrid alpha-amylase [pTUBE638-alpha-amylase (E24)] was purified from the periplasm of E. coli(pTUBE638) as the substrate for the in vitro processing reaction, in which a 21-amino-acid extra-peptide was added at the NH2...

journal_title：Journal of biochemistry

authors： Itoh Y,Sumi M,Nakamura K,Yamane K

更新日期：1990-12-01 00:00:00

abstract：:To investigate the fine substrate specificities of four highly purified exo-type cellulases (Exo-A from Aspergillus niger, CBHI and CBHII from Trichoderma reesei, and Ex-1 from Irpex lacteus), water-soluble substrates such as barley glucan, xyloglucan from tamarind (Tamarindus indica L.), and their oligosaccharides we...

journal_title：Journal of biochemistry

authors： Amano Y,Shiroishi M,Nisizawa K,Hoshino E,Kanda T

更新日期：1996-12-01 00:00:00

abstract：:The interaction between n-octyl-beta-D-glucopyranoside (octyl glucoside) and bovine liver glutamate dehydrogenase (GDH) was studied using techniques including equilibrium dialysis, UV-spectrophotometry, circular dichroism (CD), fluorescence energy transfer and extrinsic spectrofluorometry in 50 mM sodium phosphate buf...

journal_title：Journal of biochemistry

authors： Ghobadi S,Safarian S,Moosavi-Movahedi AA,Ranjbar B

更新日期：2001-11-01 00:00:00

abstract：:Inosine triphosphate (ITP) does not serve as a substrate for myosin light-chain kinase from gizzard muscle. That is to say, myosin light-chain is not phosphorylated in ITP media. Nevertheless, at pH 6.8, 1 mM or 5 mM ITP induces superprecipitation of skeletal acto-gizzard myosin. The ITP-induced superprecipitation occ...

journal_title：Journal of biochemistry

authors： Onishi H,Watanabe S

更新日期：1979-08-01 00:00:00

abstract：:We prepared human rheumatoid arthritic synovial fluid phospholipase A2 (PLA2) [EC 3.1.1.4] from insect cells infected with a recombinant baculovirus. The PLA2 DNA was designed, changing the original codons to those used frequently in the polyhedrin gene. Sixteen oligo-deoxynucleotides ranging from 40 to 70 nucleotides...

journal_title：Journal of biochemistry

authors： Kawauchi Y,Takasaki J,Matsuura Y,Masuho Y

更新日期：1994-07-01 00:00:00

abstract：:An inhibitor of neutral subtilopeptidase [EC 3.4.24.4] was purified from porcine serum by salting out with (NH4)2SO4, chromatography on anion exchange sephadex, gel filtration with Sepharose 6B, and isoelectric focusing. The preparation was homogeneous by electrophoretic and ultracentrifugal criteria, and was shown to...

journal_title：Journal of biochemistry

authors： Tsuru D,Tomimatsu M,Fujiwara K,Kawahara K

更新日期：1975-06-01 00:00:00

abstract：:Ribosomal protein S19 (RP S19) oligomers have been discovered as the first chemoattractant of migrating monocytes/macrophages to apoptotic cells via the C5a receptor (C5aR). In contrast to C5a, a fusion of the C-terminus (I(134)-H(145)) of RP S19 to C5a, the C5a/RP S19 chimera, substitutes for the RP S19 oligomers and...

journal_title：Journal of biochemistry

authors： Nishiura H,Zhao R,Yamamoto T

更新日期：2011-09-01 00:00:00

abstract：:Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin na...

journal_title：Journal of biochemistry

authors： Oda T,Ito A

更新日期：1981-05-01 00:00:00

abstract：:Rat platelets released phospholipase A2 and lysophospholipase upon activation with thrombin or ADP. The release of phospholipases was energy-dependent and was not in parallel with that of a known lysosomal marker enzyme, N-acetyl-beta-D-glucosaminidase. The phospholipases are derived from other granules (dense granule...

journal_title：Journal of biochemistry

authors： Horigome K,Hayakawa M,Inoue K,Nojima S

更新日期：1987-01-01 00:00:00

abstract：:Ras is a signal-transducing, guanine nucleotide-binding protein for various membrane receptors including tyrosine kinase receptors. Ras participates in the regulation of cell proliferation, differentiation, and morphology. Activated ras oncogenes have been identified in various forms of human cancer including epitheli...

journal_title：Journal of biochemistry

authors： Yamamoto T,Taya S,Kaibuchi K

更新日期：1999-11-01 00:00:00

abstract：:Diacylglycerol acyltransferase (DGAT) was examined as a key enzyme for triacylglycerol (TG) accumulation of an oleaginous fungus, Mortierella ramanniana var. angulispora. Subcellular fractionation of the fungus showed that DGAT activity was highest in the lipid body fraction, which occupied 77% of the recovered DGAT a...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Nakahara T

更新日期：1994-12-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:Temperature and solvent effects on reaction center structures were examined in two thermophilic photosynthetic bacteria, Chloroflexus aurantiacus and Chromatium tepidum, in order to gain insight into the interactions among the reaction center proteins and pigment systems. Thermal stability of the reaction centers was ...

journal_title：Journal of biochemistry

authors： Nozawa T,Madigan MT

更新日期：1991-10-01 00:00:00

abstract：:NADP+-specific isocitrate dehydrogenase was found in several tissues of the pupa of the silkworm, Bombyx mori. This enzyme was highly purified from the whole bodies of pupae. This is the first isolation of the enzyme from insect materials. The purified enzyme gave a single protein band on polyacrylamide gel electropho...

journal_title：Journal of biochemistry

authors： Miake F,Torikata T,Koga K,Hayashi K

更新日期：1977-08-01 00:00:00

abstract：:1. Glyceraldehyde-3-phosphate dehydrogenases [EC 1.2.1.12] from rabbit, pig, lobster, yeast, E. coli, and B. stearothermophilus have been subjected to hybridization in 3M NaCL. 2. Suitable mixtures of electrophoretically distinct glyceraldehyde-3-phosphate dehydrogenases were found to give five-membered by electrophor...

journal_title：Journal of biochemistry

authors： Suzuki K,Harris JI

更新日期：1975-03-01 00:00:00

abstract：:A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state vi...

journal_title：Journal of biochemistry

authors： Nishida E,Kuwaki T,Maekawa S,Sakai H

更新日期：1981-05-01 00:00:00

abstract：:We evaluated the role of cytosolic phospholipase A2 (PLA2) in the exocytosis of amylase from parotid acinar cells. The exocytosis stimulated by isoproterenol was dose-dependently inhibited by bromoenol lactone (BEL), a potent suicide inhibitor of Ca2+-independent cytosolic PLA2. The IC50 value of BEL was approximately...

journal_title：Journal of biochemistry

authors： Takuma T,Ichida T

更新日期：1997-06-01 00:00:00

abstract：:Heptic fibrosis/cirrhosis is a common hepatic disease characterized by the hyper-accumulation of connective tissue components, and hepatic necrosis. Chronic alcohol ingestion, viral infection, and metabolic disorders are contributing factors and there has been no effective treatment. Hepatocyte growth factor (HGF), or...

journal_title：Journal of biochemistry

authors： Matsuda Y,Matsumoto K,Ichida T,Nakamura T

更新日期：1995-09-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:The complete amino acid sequence and disulfide bridge location of HR2a, one of the hemorrhagic proteins isolated from the snake venom of Trimeresurus flavoviridis, have been determined by analysis of peptides derived from digests with cyanogen bromide, lysyl endopeptidase, trypsin, and Staphylococcus aureus V8 proteas...

journal_title：Journal of biochemistry

authors： Miyata T,Takeya H,Ozeki Y,Arakawa M,Tokunaga F,Iwanaga S,Omori-Satoh T

更新日期：1989-05-01 00:00:00

abstract：:Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

journal_title：Journal of biochemistry

authors： Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

更新日期：1981-05-01 00:00:00

abstract：:By monitoring the activation of protein C and the regulation of factor Xa-catalyzed thrombin formation by the activated protein C (APC) on the surface of human umbilical vein endothelial cells (HUVEC), we found that functional protein C was synthesized in cultured HUVEC and expressed thereon in the presence of vitamin...

journal_title：Journal of biochemistry

authors： Tanabe S,Sugo T,Matsuda M

更新日期：1991-06-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:The beta peptide (113-125), derived from a conserved sequence of the beta subunit of integrins, was synthesized to investigate its adhesive properties to matrix proteins and the effects on cell adhesion to immobilized fibronectin. In this study, we observed that the biotinylated beta peptide was able to bind efficient...

journal_title：Journal of biochemistry

authors： Liu YK,Nemoto A,Feng Y,Uemura T

更新日期：1997-05-01 00:00:00

abstract：:Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organell...

journal_title：Journal of biochemistry

authors： Moriyama Y,Manabe T,Yoshimori T,Tashiro Y,Futai M

更新日期：1994-02-01 00:00:00