Abstract:
:An inhibitor of neutral subtilopeptidase [EC 3.4.24.4] was purified from porcine serum by salting out with (NH4)2SO4, chromatography on anion exchange sephadex, gel filtration with Sepharose 6B, and isoelectric focusing. The preparation was homogeneous by electrophoretic and ultracentrifugal criteria, and was shown to be a glycoprotein with a molecular weight of 740,000. It inhibited the caseinolytic activities of thermolysin, subtilisin, trypsin [EC 3.4.21.4], and alpha-chymotrypsin [EC 3.4.21.1] as well as that of neutral subtilopeptidase by an equimolar binding to those proteolytic enzymes. SDS-polyacrylamide gel electrophoresis after reduction with beta-mercaptoethanol indicated that the inhibitor was made up of four subunit monomers having a molecular weight of 190,000. From comparisons of its physiocochemical and inhibitory properties with those of well-investigated plasma proteins, the inhibitor was identified as alpha2-macroglobulin. On treatment of the inhibitor with neutral subtilopeptidase, a protein with a molecular weight of 95,000 appeared after treatment with SDS and beta-mercaptoethanol, suggesting that a peptide bond susceptible to the enzyme exists near the mid-point of the subunit chains.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsuru D,Tomimatsu M,Fujiwara K,Kawahara Ksubject
Has Abstractpub_date
1975-06-01 00:00:00pages
1305-12issue
6eissn
0021-924Xissn
1756-2651journal_volume
77pub_type
杂志文章abstract::Affinity chromatography based on the reaction between SH groups in protein and +HgC6H4CO groups in the p-mercuribenzoylaminoethyl derivative of Sepharose 4B was examined with a crude preparation of calf thymus cysteine-containing histone. Adsorption of the histone onto the column by specific coupling was found to be ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132081
更新日期:1978-06-01 00:00:00
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更新日期:1984-03-01 00:00:00
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更新日期:1976-06-01 00:00:00
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更新日期:1988-08-01 00:00:00
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更新日期:1979-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122661
更新日期:1989-02-01 00:00:00
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更新日期:2013-02-01 00:00:00
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更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1975-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-03-01 00:00:00