Binding of connectin to myosin filaments.

abstract：:Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

journal_title：Journal of biochemistry

authors： Udagawa O,Ishihara N

更新日期：2020-03-01 00:00:00

abstract：:Kinetic studies of the two beta-glucosidase isozymes, GB-1 and GB-2, which were from the culture filtrate of a phytopathogenic fungus Pyricularia oryzae, revaled that the latter isozyme was an allosteric protein with two substrate binding sites. The homotropic effects of o- and m-nitrophenyl-beta-D-glucosides on GB-2 ...

journal_title：Journal of biochemistry

authors： Hirayama T,Nagayama H,Matsuda K

更新日期：1980-04-01 00:00:00

abstract：:Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

journal_title：Journal of biochemistry

authors： Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

更新日期：2001-04-01 00:00:00

abstract：:Chain elongation of fusaric acid and related compounds in the presence of rat liver preparations was investigated by gas chromatography-mass spectrometry. The mitochondrial fraction catalyzed the elongation of the CoA esters of fusaric acid and 5-butyl-2-pyrimidinecarboxylic acid utilizing acetyl-CoA as a C2 donor. Th...

journal_title：Journal of biochemistry

authors： Mizugaki M,Sagi M,Yamanaka H,Takayama H,Ishibashi M,Miyazaki H

更新日期：1986-02-01 00:00:00

abstract：:The position of a single N'-formylkynurenine residue in ozone-inactivated hen egg-white lysozyme [EC 3.2.1.17] was determined by two methods. One involved identification of an amino-terminus of the C-peptide obtained by selective cleavage with hydrazine of the kynurenyl peptide linkage in oxidized lysozyme. The other ...

journal_title：Journal of biochemistry

authors： Sakiyama F,Natsuki R

更新日期：1976-01-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00

abstract：:The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...

journal_title：Journal of biochemistry

authors： Nakanishi N,Hasegawa H,Akino M,Yamada S

更新日期：1986-03-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00

abstract：:Adult rat hepatocytes in primary culture cannot survive more than 2 days in the absence of calf serum. An extract of bovine pituitary gland had a similar effect to calf serum on the survival of hepatocytes, but its specific activity was 70 times that of calf serum. The survival factor was purified from bovine pituitar...

journal_title：Journal of biochemistry

authors： Asami O,Nakamura T,Mura T,Ichihara A

更新日期：1984-02-01 00:00:00

abstract：:A group B streptococcus, Streptococcus agalactiae type Ib, produces a high-molecular-weight polysaccharide consisting of the following pentasaccharide repeating unit: -->4)-[alpha-D-NeupNAc-(2-->3)-beta-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->3)]-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->. The type-specific capsular polysacchar...

journal_title：Journal of biochemistry

authors： Watanabe M,Miyake K,Yanae K,Kataoka Y,Koizumi S,Endo T,Ozaki A,Iijima S

更新日期：2002-02-01 00:00:00

abstract：:Puromycin-mediated in vitro release of nascent peptides from rat liver ribosomes was significantly stimulated by the presence of low concentrations of a detergent, and the stimulation was much more marked with bound ribosomes than with free ribosomes. The release of nascent peptides from ribosomes could be carried out...

journal_title：Journal of biochemistry

authors： Oda T,Nabi N,Omura T

更新日期：1981-03-01 00:00:00

abstract：:By using commercially available anhydrous hydrazine in the gas-phase, mucin-type oligosaccharides were released from porcine gastric mucin (PGM) and bovine fetuin. The data indicated that a certain amount of the oligosaccharides from PGM were further degraded. Despite this, the HPLC elution profile of the anthranilic ...

journal_title：Journal of biochemistry

authors： Goso Y,Tsubokawa D,Ishihara K

更新日期：2009-06-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:Electron transport particles (ETP) prepared from beef heart mitochondria formed malondialdehyde by NADPH-dependent lipid peroixidation in the presence of ferric ions and ADP or ATP. The reaction was inhibited by MnCl2, EDTA, or radical scavengers, but was not inhibited by p-hydroxymercuribenzoate (PHMB) or respiratory...

journal_title：Journal of biochemistry

authors： Takeshige K,Minadami S

更新日期：1975-05-01 00:00:00

abstract：:Some alpha(1,3)fucosylated oligosaccharides serve as counter receptors to lectin-like adhesion proteins or are expressed with temporal precision during embryogenesis, and alpha(1, 3)fucosyltransferase is a key enzyme in the production of these oligosaccharides. Two alpha(1,3)-fucosyltransferase genes, designated zFT1 ...

journal_title：Journal of biochemistry

authors： Kageyama N,Natsuka S,Hase S

更新日期：1999-04-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:Low molecular weight RNAs were isolated from nuclei of the cellular slime mold Dictyostelium discoideum AX-3. Analysis of the RNAs by polyacrylamide gel electrophoresis showed that the vegetative cell nuclei contained, besides tRNA (Dd1), 5S RNA (Dd4), and 5.8S RNA (Dd7), at least 7 small RNA species (Dd3, Dd5, Dd6, D...

journal_title：Journal of biochemistry

authors： Takeishi K,Kaneda S

更新日期：1981-08-01 00:00:00

abstract：:The vitamin A derivative, retinoic acid (RA) has various biological effects in mammalian cells and tissues. It is well known that RA induces differentiation of leukemia cells and inhibits cell growth. There are two pathways for RA action; one via RA nuclear receptors (RARs), and one via acylation of proteins by RA (re...

journal_title：Journal of biochemistry

authors： Kubo Y,Ohba T,Takahashi N

更新日期：2008-09-01 00:00:00

abstract：:The organization of troponin I (TnI) genes from the ascidian Halocynthia roretzi have been determined. Halocynthia possesses roughly two types of TnI isoforms. One type is a single-copied adult TnI (adTnI) gene, which contains eight exons and seven introns. adTnI expresses two isoforms, the shorter body wall muscle Tn...

journal_title：Journal of biochemistry

authors： Yuasa HJ,Kawamura K,Yamamoto H,Takagi T

更新日期：2002-07-01 00:00:00

abstract：:The cDNA of a novel protein, which contains the association domain of alpha isoform of calmodulin-dependent protein kinase II (CaM-kinase II alpha), was cloned from rat skeletal muscle. This protein, called alpha KAP, consisted of 200 amino acid residues with a molecular weight of 22,583. alpha KAP has a highly hydrop...

journal_title：Journal of biochemistry

authors： Sugai R,Takeuchi M,Okuno S,Fujisawa H

更新日期：1996-10-01 00:00:00

abstract：:In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...

journal_title：Journal of biochemistry

authors： Umebayashi Y,Miyamoto Y,Wakita M,Kobayashi A,Nishisaka T

更新日期：2003-08-01 00:00:00

abstract：:When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

journal_title：Journal of biochemistry

authors： Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

更新日期：1982-06-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:An inhibitor of neutral subtilopeptidase [EC 3.4.24.4] was purified from porcine serum by salting out with (NH4)2SO4, chromatography on anion exchange sephadex, gel filtration with Sepharose 6B, and isoelectric focusing. The preparation was homogeneous by electrophoretic and ultracentrifugal criteria, and was shown to...

journal_title：Journal of biochemistry

authors： Tsuru D,Tomimatsu M,Fujiwara K,Kawahara K

更新日期：1975-06-01 00:00:00

abstract：:Comparative studies have been made on the effects of diazoacetyl-DL-norleucine methyl ester (DAN), 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and pepstatin on acid proteases, including those from Acrocylindrium sp., Aspergillus niger, Aspergillus saitoi, Mucor pusillus, Paecilomyces varioti, Rhizopus chinensis, and Tr...

journal_title：Journal of biochemistry

authors： Takahashi K,Chang WJ

更新日期：1976-09-01 00:00:00

abstract：:20S proteasomes from higher eukaryotes have immunological functions rather than those from archibacteria or yeast. To clarify the mechanism of the sorting and production of antigen-presenting peptides, it is important and worthwhile to determine the structure of mammalian proteasomes using a third generation synchrotr...

journal_title：Journal of biochemistry

authors： Tomisugi Y,Unno M,Mizushima T,Morimoto Y,Tanahashi N,Tanaka K,Tsukihara T,Yasuoka N

更新日期：2000-06-01 00:00:00

abstract：:The receptor protein for the mitochondrial protein precursor synthesized in the cytosol was extensively purified from the mitochondrial membrane fraction by affinity column chromatography using a synthetic peptide containing the extrapeptide of ornithine aminotransferase as a ligand. The purified fraction contained tw...

journal_title：Journal of biochemistry

authors： Ono H,Tuboi S

更新日期：1990-06-01 00:00:00

abstract：:We have recently demonstrated the localization of associated m-calpain and calpastatin in the endoplasmic reticulum (ER) of bovine pulmonary artery smooth muscle. Herein, we sought to determine the role of m-calpain on calcium-dependent proteolytic cleavage of Na(+)/Ca(2+) exchanger (NCX) in the ER. Treatment of the E...

journal_title：Journal of biochemistry

authors： Samanta K,Kar P,Chakraborti T,Chakraborti S

更新日期：2010-02-01 00:00:00

abstract：:We found that the glucitol concentration was extraordinarily high in bovine fetal serum, which is routinely used for cell culture in laboratories: it was as much as two orders of magnitude higher than that reported for human adult serum. We also confirmed that the serum glucitol concentration in new born babies was on...

journal_title：Journal of biochemistry

authors： Mizuno H,Akanuma H

更新日期：1993-07-01 00:00:00

abstract：:Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogeni...

journal_title：Journal of biochemistry

authors： Sakaue T,Maekawa M,Nakayama H,Higashiyama S

更新日期：2017-10-01 00:00:00