Reduced nicotinamide adenine dinucleotide phosphate-dependent lipid peroxidation by beef heart submitochondrial particles.

abstract：:Acetoxy Drug: Protein Transacetylase (TAase) mediating the transfer of acetyl group(s) from polyphenolic acetates (PA) to certain functional proteins in mammalian cells was identified by our earlier investigations. TAase activity was characterized in the cell lysates of Mycobacterium smegmatis and the purified protein...

journal_title：Journal of biochemistry

authors： Gupta G,Baghel AS,Bansal S,Tyagi TK,Kumari R,Saini NK,Ponnan P,Kumar A,Bose M,Saluja D,Patkar SA,Parmar VS,Raj HG

更新日期：2008-12-01 00:00:00

abstract：:Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however,...

journal_title：Journal of biochemistry

authors： Yamazawa R,Nakajima Y,Mushiake K,Yoshimoto T,Ito K

更新日期：2011-06-01 00:00:00

abstract：:Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related...

journal_title：Journal of biochemistry

authors： Kato S,Tsuji M,Nakanishi Y,Suzuki S

更新日期：1980-03-01 00:00:00

abstract：:The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

journal_title：Journal of biochemistry

authors： Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

更新日期：2008-10-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:Pig heart phosphoprotein phosphatase [phosphoprotein phosphophydrolase, EC 3.1.3.16] of Mr 224,000 was dissociated by gel-filtration on Sephacryl S-300, into an active subunit (alpha subunit) of Mr 31,000 and inactive subunits of higher molecular weight in the presence of 6 M urea. After the removal of urea, these sub...

journal_title：Journal of biochemistry

authors： Imazu M,Imaoka T,Usui H,Kinohara N,Takeda M

更新日期：1981-09-01 00:00:00

abstract：:Long interspersed nuclear element (LINE) is known to be transposed by reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. Our previous study revealed that the second G residue (G8) in the GGAUA loo...

journal_title：Journal of biochemistry

authors： Otsu M,Kajikawa M,Okada N,Kawai G

更新日期：2017-10-01 00:00:00

abstract：:Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated...

journal_title：Journal of biochemistry

authors： Lin CH,Hsieh M,Li YC,Li SY,Pearson DL,Pollard KM,Li C

更新日期：2000-09-01 00:00:00

abstract：:Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

journal_title：Journal of biochemistry

authors： Asayama M,Kobayashi Y

更新日期：1993-09-01 00:00:00

abstract：:Matriptase is a type II transmembrane serine protease expressed abundantly in the epithelial cells and keratinocyte. It plays a key role in the establishment and maintenance of epithelial integrity. Matriptase is considered to be at the most upstream in cellular protease cascade. Activation of its zymogen is the most ...

journal_title：Journal of biochemistry

authors： Kojima K,Inouye K

更新日期：2011-08-01 00:00:00

abstract：:Cholecalciferol 25-hydroxylase was partially purified by polyethylene glycol fractionation and chromatographies on octylamino-Sepharose and hydroxylapatite columns starting from the liver microsomes of female rats, and compared with P-450cc25 purified from the liver microsomes of male rats (Hayashi, et al. (1986) J. B...

journal_title：Journal of biochemistry

authors： Hayashi S,Usui E,Okuda K

更新日期：1988-05-01 00:00:00

abstract：:The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

journal_title：Journal of biochemistry

authors： Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

更新日期：1993-05-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:Four motor proteins, MotX, MotY, PomA, and PomB, have been identified as constituents of the Na(+)-driven flagellum of Vibrio species. In this study, the complete motX gene was cloned from Vibrio alginolyticus and shown to complement three mot mutations, motX94, motX115, and motX119, as well as a V. parahaemolyticus m...

journal_title：Journal of biochemistry

authors： Okabe M,Yakushi T,Asai Y,Homma M

更新日期：2001-12-01 00:00:00

abstract：:Two proteinaceous factors, 15K and 9K proteins, which acted together to stabilize the inactivated yeast F1F0-ATPase-inhibitor complex [Hashimoto, T., et al. (1984) J. Biochem. 95, 131-136] were hardly distinguishable from the sigma and epsilon subunits, respectively, of yeast F1-ATPase by sodium dodecyl sulfate (SDS) ...

journal_title：Journal of biochemistry

authors： Okada Y,Hashimoto T,Yoshida Y,Tagawa K

更新日期：1986-01-01 00:00:00

abstract：:The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

journal_title：Journal of biochemistry

authors： Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

更新日期：1989-03-01 00:00:00

abstract：:In order to examine the possibility of the use of a surface plasmon resonance (SPR) sensor for real-time monitoring of the process of refolding of immobilized proteins, the refolding of firefly luciferase immobilized on a carboxymethyldextran matrix layer was analyzed. The SPR signal of the immobilized luciferase decr...

journal_title：Journal of biochemistry

authors： Zako T,Harada K,Mannen T,Yamaguchi S,Kitayama A,Ueda H,Nagamune T

更新日期：2001-01-01 00:00:00

abstract：:In order to determine the epitope of metallothionein (MT) to a murine monoclonal antibody (MT 189-14-7) which had been produced by immunization with rat MT 2 (Kikuchi et al. (1988) Mol. Immunol. 25, 1033-1036), various lengths of synthetic oligopeptides were tested for their inhibitory activities in competitive radioi...

journal_title：Journal of biochemistry

authors： Kikuchi Y,Irie M,Ikebuchi H,Sawada J,Terao T,Nakayama S,Iguchi S,Okada Y

更新日期：1990-04-01 00:00:00

abstract：:Three forms of rat brain enolase (Fletcher, L., Rider, C.C., & Taylor, C.B. (1976) Biochim. Biophys. Acta 452, 245-252), which were separable by DEAE-cellulose chromatography (referred as enolase I, enolase II, and enolase III in order of the elution), were purified with a high yield by use of column chromatographies ...

journal_title：Journal of biochemistry

authors： Suzuki F,Umeda Y,Kato K

更新日期：1980-06-01 00:00:00

abstract：:Interactions between phospholipid membranes and the acyl chain and specific amino acid residues of myristoylated proteins are necessary for membrane association. In the present study we tested the effects of mutations of calcineurin B subunit amino acid residues K(20)K(21), K(24)R(25), K(27)K(28) to Glu on the interac...

journal_title：Journal of biochemistry

authors： Martin BA,Oxhorn BC,Rossow CR,Perrino BA

更新日期：2001-05-01 00:00:00

abstract：:Both beef heart cytochrome oxidase and bacteriorhodopsin of Halobacterium halobium were reconstituted into liposomes by the sonication-cholate dialysis method. The proteoliposomes showed the respiratory control ratio of 4.2, and steady-state illumination of the vesicles lead to the 2.7-fold stimulation of the oxidase ...

journal_title：Journal of biochemistry

authors： Miki T,Orii Y,Mukohata Y

更新日期：1987-07-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:ISG15 is a ubiquitin-like protein that is upregulated on treatment with interferon. ISG15 is considered to be covalently conjugated to cellular proteins through a sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1...

journal_title：Journal of biochemistry

authors： Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

更新日期：2005-12-01 00:00:00

abstract：:1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

journal_title：Journal of biochemistry

authors： Kohama K

更新日期：1979-09-01 00:00:00

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsi...

journal_title：Journal of biochemistry

authors： Matsuda T,Watanabe K,Nakamura R

更新日期：1983-07-01 00:00:00

abstract：:It was found that the absorption spectrum of beta-naphthyl triphosphate is different from that of beta-naphthyl diphosphate in the range 290-335 nm. Thus, beta-naphthyl triphosphate hydrolysis by heavy meromyosin can be recorded continuously as a function of time by means of a spectrophotometer. By analyzing the time ...

journal_title：Journal of biochemistry

authors： Kuwajima T,Kagawa H,Asai H

更新日期：1975-11-01 00:00:00