Phenyl phosphate as a water-soluble analog of dolichyl phosphate in microsomal mannosyltransferase systems.

abstract：:Reverse transcriptases (RTs) from Moloney murine leukaemia virus (MMLV) and avian myeloblastosis virus (AMV) contain all the fingers, palm, thumb, connection and RNase H domains. The fingers, palm and thumb domains are thought to be involved in the reverse transcription activity, and the RNase H domain is in the RNase...

journal_title：Journal of biochemistry

authors： Yasukawa K,Mizuno M,Inouye K

更新日期：2009-03-01 00:00:00

abstract：:Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activating proteins called (re)activases. The X-ray structures of radical enz...

journal_title：Journal of biochemistry

authors： Shibata N,Toraya T

更新日期：2015-10-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:The voltage-dependent L-type Ca2+ channel in the heart is regulated by cAMP-dependent protein kinase (PKA) and possibly by protein kinase C (PKC). We have investigated the channel modulation through phosphorylation by these protein kinases, using liposomes into which Ca2+ channels from bovine heart were reconstituted....

journal_title：Journal of biochemistry

authors： Kameyama A,Shearman MS,Sekiguchi K,Kameyama M

更新日期：1996-07-01 00:00:00

abstract：:Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells...

journal_title：Journal of biochemistry

authors： Nozawa K,Kurumiya Y,Yamamoto A,Isobe Y,Suzuki M,Yoshida S

更新日期：1999-08-01 00:00:00

abstract：:The binding of nuclear envelope precursor vesicles and chromatin was characterized by using an in vitro system constituted from a Xenopus egg extract and demembranated Xenopus sperm chromatin. The results of binding studies in the presence of salts, urea, and a chelator showed that the binding involves an ionic intera...

journal_title：Journal of biochemistry

authors： Imai N,Sasagawa S,Yamamoto A,Kikuchi F,Sekiya K,Ichimura T,Omata S,Horigome T

更新日期：1997-11-01 00:00:00

abstract：:A new p-coumaric acid (4-hydroxycinnamic acid) hydroxylase was detected in mung bean seedlings treated with tentoxin, a fungal toxin, in which polyphenol oxidase that hydroxylates a wide variety of monophenols in vitro was completely eliminated. The enzyme required molecular oxygen and showed a pH optimum of 5.0. The ...

journal_title：Journal of biochemistry

authors： Kojima M,Takeuchi W

更新日期：1989-02-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...

journal_title：Journal of biochemistry

authors： Higashi K,Kirino Y

更新日期：1983-12-01 00:00:00

abstract：:The immunological properties of gamma-glutamyltransferases (gamma-GTs) from human serum, liver and tonsil were studied by using a monospecific antibody to human kidney gamma-GT for the purpose of elucidating their isozymic relationships. gamma-GTs partially purified from liver and tonsil were indistinguishable in this...

journal_title：Journal of biochemistry

authors： Tsuchida S,Imai F,Sato K

更新日期：1981-03-01 00:00:00

abstract：:A post-proline cleaving enzyme [prolyl endopeptidase, EC 3.4.21.26] was purified about 3,700-fold from an extract of bovine brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and PCMB-T-Sepharose, and gel filtration on Sephadex G-200 using N-carbobenzoxy-Gly-Pro-beta-naphthylamide (Z-Gly-Pro...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Nishimura T,Kita T,Tsuru D

更新日期：1983-10-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...

journal_title：Journal of biochemistry

authors： Abe T,Kawarai T,Takahashi Y,Konishi K

更新日期：2017-06-01 00:00:00

abstract：:Thioredoxin 1 (Trx1) is known to play an important role in protecting against cell death. However, the mechanism for control of Trx1 in cell death resulting from DNA damage has not been fully investigated. In this study, we used the DNA-damaging agent methyl methanesulfonate (MMS) to investigate the protective effects...

journal_title：Journal of biochemistry

authors： Gu L,Gao W,Yang HM,Wang BB,Wang XN,Xu J,Zhang H

更新日期：2016-01-01 00:00:00

abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Katsuda N,Kato K

更新日期：1980-02-01 00:00:00

abstract：:To determine the relationship between the induction patterns of three components of beta-glucosidase of Pyricularia oryzae and carbon sources in the growth medium, various culture conditions were examined. Avicel, hydroxyethylcellulose and methyl-beta-D-glucoside as the carbon source induced both beta-glucosidase comp...

journal_title：Journal of biochemistry

authors： Hirayama T,Nagayama H,Matsuda K

更新日期：1979-02-01 00:00:00

abstract：:We have analyzed the manner of incorporation of bile acid into lipid bilayers and resultant perturbation of the bilayer structure with lower bile acid/lipid ratios relevant to the physiological conditions (approximately 1 mM) by 2H and 31P NMR methods, as an aid to understanding the possible role as an endogenous tumo...

journal_title：Journal of biochemistry

authors： Saitŏ H,Sugimoto Y,Tabeta R,Suzuki S,Izumi G,Kodama M,Toyoshima S,Nagata C

更新日期：1983-12-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:A new sulfated polysaccharide was isolated from the culture supernatant of a strain of Arthrobacter sp. The polysaccharide purified with quaternary ammonium salts consists of D-galactose, D-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and LL-diaminopimelic acid in a molar r...

journal_title：Journal of biochemistry

authors： Inoue K,Korenaga H,Kadoya S

更新日期：1982-12-01 00:00:00

abstract：:Heat-stable L-lactate dehydrogenase [EC 1.1.1.27] was purified from an extremely thermophilic bacterium belonging to the genus Thermus, and it showed an allosteric nature dependent on fructose 1,6-bisphosphate as an effector. The enzyme had a molecular weight of approximately 120,000 with a subunit molecular weight of...

journal_title：Journal of biochemistry

authors： Taguchi H,Yamashita M,Matsuzawa H,Ohta T

更新日期：1982-04-01 00:00:00

abstract：:The receptor protein for the mitochondrial protein precursor synthesized in the cytosol was extensively purified from the mitochondrial membrane fraction by affinity column chromatography using a synthetic peptide containing the extrapeptide of ornithine aminotransferase as a ligand. The purified fraction contained tw...

journal_title：Journal of biochemistry

authors： Ono H,Tuboi S

更新日期：1990-06-01 00:00:00

abstract：:We previously reported that novel Mg(2+)-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associat...

journal_title：Journal of biochemistry

authors： Shimizu S,Imanaka T,Takano T,Ohkuma S

更新日期：1992-12-01 00:00:00

abstract：:The aromatic region of the NMR spectrum of bovine pancreatic ribonuclease A was analyzed in order to clarify the nature of the microenvironments surrounding the individual histidine, tyrosine, and phenylalanine residues and the interactions with inhibitors. The NMR titration curves of ring protons of six tyrosine and ...

journal_title：Journal of biochemistry

authors： Tanokura M

更新日期：1983-07-01 00:00:00

abstract：:We have studied the regio- and stereoselectivity of ring-hydroxylation and N-desisopropylation of S(-)- and R(+)-propranolol, using dog liver microsomes and the expressed dog CYP2D15 in insect cells. In dog liver microsomes, 4-hydroxylation was the preferred pathway in S(-)-propranolol oxidation, while N-desisopropyla...

journal_title：Journal of biochemistry

authors： Tasaki T,Iwata H,Kazusaka A,Fujita S

更新日期：1998-04-01 00:00:00

abstract：:Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin is a Ca2+-dependent regulator of muscle contraction. Both proteins are widely distributed in protostomes, while in deuterostomes, their distribution is limited; namely, presence of paramyosin and absence of troponin are commo...

journal_title：Journal of biochemistry

authors： Sonobe H,Obinata T,Minokawa T,Haruta T,Kawamura Y,Wakatsuki S,Sato N

更新日期：2016-12-01 00:00:00

abstract：:The complete amino acid sequence of ribonuclease N1 (RNase N1), a guanine-specific ribonuclease from a fungus, Neurospora crassa, was determined by conventional protein sequencing, using peptide fragments obtained by tryptic digestion of cyanogen bromide-treated RNase N1 and by Staphylococcus aureus V8 protease digest...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1988-09-01 00:00:00

abstract：:Synthesis of the acid-soluble proteins in the early cleavage stage of the sea urichin, Hemicentrotus pulcherrimus, was investigated. As detected by the incorporation of lysine, the acid-soluble proteins were synthesized periodically even before the first cleavage, differing from the pattern of incorporation of tryptop...

journal_title：Journal of biochemistry

authors： Kano K,Mano Y

更新日期：1976-09-01 00:00:00

abstract：:It has been demonstrated that the GM2 ganglioside cannot be cleaved by exo-beta-N-acetylhexosaminidases isolated from molds or bacterial sources. Here, a novel GM2 ganglioside-degrading enzyme was found in cells of Nocardia sp. This enzyme releases free fatty acids from the GM2 ganglioside. The chemical structure of t...

journal_title：Journal of biochemistry

authors： Hirabayashi Y,Kimura M,Matsumoto M,Yamamoto K,Kadowaki S,Tochikura T

更新日期：1988-01-01 00:00:00

abstract：:Light harvesting (LH) bacteriochlorophyll (Bchl) protein complexes were isolated from chromatophores of Chromatium vinosum, by the combination of detergent solubilization, sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and hydroxyapatite chromatography. Addition of the absorption spectra of thes...

journal_title：Journal of biochemistry

authors： Hayashi H,Morita S

更新日期：1980-11-01 00:00:00

abstract：:Seven ganglioside species were isolated and purified from erythrocyte membranes of Japanese cats by DEAE-Sephadex and Iatrobeads column chromatographies. The structures of these gangliosides were determined as Gmi(NeuGc), Gm3(NeuAc), GM3(NeuGc), GD3(NeuGc), GD3(NeuGc comes from NeuAc), GT3(NeuGc), and another GM3 cont...

journal_title：Journal of biochemistry

authors： Ando N,Yamakawa T

更新日期：1982-03-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00