A sulfated polysaccharide produced by an Arthrobacter species.

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state vi...

journal_title：Journal of biochemistry

authors： Nishida E,Kuwaki T,Maekawa S,Sakai H

更新日期：1981-05-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-ac...

journal_title：Journal of biochemistry

authors： Kobayashi K,Morisawa Y,Ishituka T,Ishimoto M

更新日期：1975-11-01 00:00:00

abstract：:The binding properties of dynein arms to the A- and B-tubules of outer doublets of cilia from Tetrahymena pyriformis were examined, with the following results: 1. When 30s dynein purified from Tetrahymena cilia was added to doublets deficient in dynein arms, it bound to both A- and B-tubules almost equally and formed ...

journal_title：Journal of biochemistry

authors： Takahashi M,Tonomura Y

更新日期：1978-12-01 00:00:00

abstract：:The nucleotide sequence of IStaqTZ2 are available in the DDBJ/EMBL/GenBank databases under the accession number AB063392. A novel insertion sequence (IStaqTZ2) was transposed from the genome of Thermus thermophilus TZ2 to that of the thermophilic bacteriophage IN93. The complete nucleotide sequence of IStaqTZ2 was det...

journal_title：Journal of biochemistry

authors： Matsushita I,Yanase H

更新日期：2009-12-01 00:00:00

abstract：:To study the structure/function relationship and enzymatic properties of human aldolase C, we have constructed an Escherichia coli expression plasmid, pHAC11, for the isozyme. E. coli cells carrying this plasmid produced enzymatically active human aldolase C. The kcat and Km values for fructose-1,6-bisphosphate (Fru-1...

journal_title：Journal of biochemistry

authors： Kusakabe T,Motoki K,Hori K

更新日期：1994-06-01 00:00:00

abstract：:Carboxyl groups of native human fibrinogen were modified with glycine methyl ester and 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. It seemed likely that the modification occurred stepwise. Approximately 26% of the carboxyl groups of fibrinogen was modified finally. The modified fibrinogen had no interaction with cat...

journal_title：Journal of biochemistry

authors： Kurioka S,Inoue F,Matsuda M

更新日期：1975-11-01 00:00:00

abstract：:Three kinds of phospholipase C [EC 3.1.4.3] were used to selectively hydrolyze phospholipids in rat liver microsomes, and their effects on the acyl-CoA: glycerophosphate and acyl-CoA: lysophospholipids acyltransferase systems were examined. The glycerophosphate acyltransferase [EC 2.3.1.15] system was inactivated rapi...

journal_title：Journal of biochemistry

authors： Sonoki S,Ikezawa H

更新日期：1976-12-01 00:00:00

abstract：:The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Norioka S,Teshima T,Ikenaka T

更新日期：1987-03-01 00:00:00

abstract：:The technique of saturation transfer electron spin resonance has been applied to study the rotational diffusion of spin-labeled Ca2+, Mg2+-dependent ATPase molecules in the membranes of sarcoplasmic reticulum vesicles. Comparison of the present data with those for spin-labeled hemoglobin undergoing isotropic rotation ...

journal_title：Journal of biochemistry

authors： Kirino Y,Ohkuma T,Shimizu H

更新日期：1978-07-01 00:00:00

abstract：:The interaction between pullulanase from Klebsiella pneumoniae and alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was examined by means of inhibition studies of the enzyme activity, UV difference spectroscopy, and flow calorimetry. All the abov...

journal_title：Journal of biochemistry

authors： Iwamoto H,Ohmori M,Ohno M,Hirose J,Hiromi K,Fukada H,Takahashi K,Hashimoto H,Sakai S

更新日期：1993-01-01 00:00:00

abstract：:Glycosphingolipids (GSLs), cell type-specific markers which change dramatically during ontogenesis and oncogenesis, have been implicated as playing major roles in cellular interactions and control of cell proliferation in multicellular organisms. These functional roles have been partially clarified through two types o...

journal_title：Journal of biochemistry

authors： Hakomori S,Igarashi Y

更新日期：1995-12-01 00:00:00

abstract：:Amyloid beta peptide (Abeta) is a physiological peptide that is constantly catabolized in the brain. We previously demonstrated that an endopeptidase sensitive to phosphoramidon and thiorphan conducts the initial rate-limiting proteolysis of Abeta in vivo, but the exact molecular identity of the peptidase(s) has remai...

journal_title：Journal of biochemistry

authors： Takaki Y,Iwata N,Tsubuki S,Taniguchi S,Toyoshima S,Lu B,Gerard NP,Gerard C,Lee HJ,Shirotani K,Saido TC

更新日期：2000-12-01 00:00:00

abstract：:The gene of an extreme thermophile, Thermus thermophilus HB8, which codes for a leucine biosynthetic enzyme, 3-isopropylmalate (3-IPM) dehydrogenase [EC 1.1.1.85], was cloned in Escherichia coli using pBR322 as a vector. E. coli cells carrying this recombinant plasmid, pHB2, produced the thermophilic enzyme 7-fold mor...

journal_title：Journal of biochemistry

authors： Tanaka T,Kawano N,Oshima T

更新日期：1981-02-01 00:00:00

abstract：:Comparative studies of the unfolding equilibria of two homologous proteins, bovine alpha-lactalbumin and hen lysozyme, induced by treatment with guanidine hydrochloride have been made by analysis of the peptide and the aromatic circular dichroism spectra. The effect of the specific binding of Ca2+ ion by the former pr...

journal_title：Journal of biochemistry

authors： Ikeguchi M,Kuwajima K,Sugai S

更新日期：1986-04-01 00:00:00

abstract：:Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

journal_title：Journal of biochemistry

authors： Seki-Chiba S,Ishimoto M

更新日期：1977-12-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynam...

journal_title：Journal of biochemistry

authors： Liu J,Song Y,Tian B,Qian J,Dong Y,Liu J,Liu B,Sun Z

更新日期：2010-12-01 00:00:00

abstract：:We tested the capsid targeted viral inactivation method as an anti-HBV strategy. HepG2 cells were cotransfected with HBV expression plasmid and the plasmid encoding fusion protein of either Core-A3C or Core-humanized renilla GFP (hrGFP). Core-A3C had substantial effect on HBV DNA levels. In the HepG2 cells expressing ...

journal_title：Journal of biochemistry

authors： Li D,Liu J,Kang F,Guan W,Gao X,Wang Y,Sun D

更新日期：2011-10-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00

abstract：:The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or ...

journal_title：Journal of biochemistry

authors： Takigawa M,Watanabe R,Ishida H,Asada A,Suzuki F

更新日期：1979-01-01 00:00:00

abstract：:Effects of polydeoxynucleotides on the activity of DNA polymerase-alpha from sea urchin embryos were studied. Poly(dG), poly(dC) and poly(dC)-oligo(dG)12-18 inhibited DNA polymerase-alpha activity in the activated DNA-directed reaction but poly(dA), oligo(dT)12-18, and poly(dA)-oligo(dT)12-18 did not inhibit the activ...

journal_title：Journal of biochemistry

authors： Oguro M,Nagano H

更新日期：1982-08-01 00:00:00

abstract：:[3H]Methyl group incorporation and histamine secretion in rat mast cells induced by anti-IgE and con A were strongly inhibited by trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester (GMCHA-OPhBut), a strong and specific inhibitor for pH 7 tryptase (Muramatsu et al. (1988) Biol. Chem. Hoppe-Seyle...

journal_title：Journal of biochemistry

authors： Takei M,Matumoto T,Endo K,Muramatu M

更新日期：1989-02-01 00:00:00

abstract：:We have isolated cDNAs and completed for the first time the primary structure for a novel collagenous chain that was partially characterized earlier and named alpha 1(Y) chain [Yoshioka, H. et al. (1992) Genomics 13, 884-886]. The size of the coding region was unexpectedly small compared with the length of the mRNA (>...

journal_title：Journal of biochemistry

authors： Inoguchi K,Yoshioka H,Khaleduzzaman M,Ninomiya Y

更新日期：1995-01-01 00:00:00

abstract：:A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

journal_title：Journal of biochemistry

authors： Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

更新日期：1983-12-01 00:00:00

abstract：:Aorta smooth muscle myosin contains two kinds of 17-kDa essential light chain, LC17nm (nonmuscle-type) and LC17gi (gizzard-type) [Hasegawa, Y., Ueda, Y., Watanabe, M., & Morita, F. (1992) J. Biochem. 111, 798-803]. The LC17 isoforms were released from porcine aorta myosin by incubation at 46 degrees C. The rate of rel...

journal_title：Journal of biochemistry

authors： Hasegawa Y,Morita F

更新日期：1992-06-01 00:00:00

abstract：:The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...

journal_title：Journal of biochemistry

authors： Murata D,Arai K,Iijima M,Sesaki H

更新日期：2020-03-01 00:00:00

abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Katsuda N,Kato K

更新日期：1980-02-01 00:00:00

abstract：:A DNA polymerase beta (pol. beta) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-beta-D-glucoside, prunasin. The compound inhib...

journal_title：Journal of biochemistry

authors： Mizushina Y,Takahashi N,Ogawa A,Tsurugaya K,Koshino H,Takemura M,Yoshida S,Matsukage A,Sugawara F,Sakaguchi K

更新日期：1999-08-01 00:00:00