Biochemical identification of the neutral endopeptidase family member responsible for the catabolism of amyloid beta peptide in the brain.

abstract：:Genomic DNA libraries were screened for the human histidine-rich glycoprotein (HRG) gene and a sequence of 15,499 nucleotides was determined. The gene is composed of 7 exons and 6 introns, and all the exon-intron boundaries match the consensus GT/AG sequence for donor and acceptor splice sites. Each of cystatin-like d...

journal_title：Journal of biochemistry

authors： Wakabayashi S,Takahashi K,Koide T

更新日期：1999-03-01 00:00:00

abstract：:Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in t...

journal_title：Journal of biochemistry

authors： Yamada H,Uozumi F,Ishikawa A,Imoto T

更新日期：1984-02-01 00:00:00

abstract：:Studies on the solution structure dynamics of RNA/DNA are becoming crucially important. The phenomena of SSCP (single-strand conformation polymorphism), small RNA dynamics in a cell, and others can be related to the conformational changes of single-stranded (ss) RNAs/DNAs in solution. However, little is known about th...

journal_title：Journal of biochemistry

authors： Biyani M,Nishigaki K

更新日期：2005-10-01 00:00:00

abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...

journal_title：Journal of biochemistry

authors： Waki H,Murata A,Kon K,Maruyama K,Kimura S,Ogura H,Ando S

更新日期：1993-04-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...

journal_title：Journal of biochemistry

authors： Hagiwara K,Hara H,Hanada K

更新日期：2013-02-01 00:00:00

abstract：:The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is ...

journal_title：Journal of biochemistry

authors： Sakaguchi A,Miyaike M,Kuroda K,Nozaki N,Tanaka M,Hibino M,Fujii Y,Kato S,Kikuchi A

更新日期：2002-09-01 00:00:00

abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

journal_title：Journal of biochemistry

authors： Ohe Y,Hayashi H,Iwai K

更新日期：1979-02-01 00:00:00

abstract：:The protein structure, kinin-releasing activity, and tissue distribution of four major proteinases of mouse submandibular gland (mK22, mK9, proteinase F, proteinase P) were studied. When compared with the deduced amino acid sequence of each member of the tissue (glandular) kallikrein gene family, the amino acid sequen...

journal_title：Journal of biochemistry

authors： Hosoi K,Tsunasawa S,Kurihara K,Aoyama H,Ueha T,Murai T,Sakiyama F

更新日期：1994-01-01 00:00:00

abstract：:Bacterial flagellar motors exploit the electrochemical potential gradient of a coupling ion as energy source and are composed of stator and rotor proteins. Vibrio alginolyticus has a Na(+)-driven motor and its stator is composed of PomA and PomB. Recently, we isolated increased motility strains (sp1-sp4) from the PomA...

journal_title：Journal of biochemistry

authors： Terashima H,Terauchi T,Ihara K,Nishioka N,Kojima S,Homma M

更新日期：2013-08-01 00:00:00

abstract：:The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...

journal_title：Journal of biochemistry

authors： Miyanishi T,Toyoshima C,Wakabayashi T,Matsuda G

更新日期：1988-03-01 00:00:00

abstract：:The differential assay conditions for protein phosphatases PP1, PP2A, and PP2C were extensively studied by using crude extracts from mouse lymphoid tissues as enzyme sources. Under these conditions, the protein phosphatase activities were measured in MRL/MpJ-lpr/lpr mice (MRL/lpr mice), autoimmune-prone mice, and MRL/...

journal_title：Journal of biochemistry

authors： Matsuzawa S,Tamura T,Mizuno Y,Kobayashi S,Okuyama H,Tsukitani Y,Uemura D,Kikuchi K

更新日期：1992-04-01 00:00:00

abstract：:In regenerating rat liver, nuclear protein histone H2A was shown to be phosphorylated on its amino-terminal serine residue [Sung et al. (1971) J. Biol. Chem. 246, 1358-1364], but the protein kinase which phosphorylates this residue has not been identified. To evaluate the possibility that protein kinase C can phosphor...

journal_title：Journal of biochemistry

authors： Takeuchi F,Hashimoto E,Yamamura H

更新日期：1992-06-01 00:00:00

abstract：:Hepatic peroxisomes and mitochondria from 20-day-old chick embryo were separated by sucrose density gradient centrifugation and the characteristics of carnitine acyltransferases in these organelles were studied. The carnitine acyltransferase activities in peroxisomes were increased markedly by the treatment of chick e...

journal_title：Journal of biochemistry

authors： Ishii S,Ishii H,Suga T

更新日期：1985-09-01 00:00:00

abstract：:A 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) binding site of mouse mastocytoma P815 cell membranes has been purified approximately 100-fold by affinity chromatography. This adenosine binding site, which has a similar specificity to that of the A2 adenosine receptor, was absorbed on NECA-linked Sepharose 6B and elut...

journal_title：Journal of biochemistry

authors： Nakata H

更新日期：1989-05-01 00:00:00

abstract：:Neutral alpha-glucosidase was partially purified from granular fractions isolated from guinea pig polymorphonuclear leukocytes (PMNL). The native enzyme had a high molecular weight, about 417,000, with a subunit of 43,000. The purified enzyme hydrolysed 4-methylumbelliferyl alpha-glucoside and maltose, but not isomalt...

journal_title：Journal of biochemistry

authors： Imai K,Harada T,Takano Y,Morikawa S,Tanaka A

更新日期：1989-10-01 00:00:00

abstract：:Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

journal_title：Journal of biochemistry

authors： Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

更新日期：2003-12-01 00:00:00

abstract：:Both rabbit liver microsomes and reconstituted system with purified cytochrome P-450 and cofactors enzymatically oxidized o-dithiane (1, 2-dithiane), 3-methyl-o-dithiane, thiane and 2-methylthiane to the corresponding mono-oxygenated products; sulfides or disulfides were oxidized to the corresponding sulfoxides or thi...

journal_title：Journal of biochemistry

authors： Fukushima D,Kim YH,Iyanagi T,Oae S

更新日期：1978-04-01 00:00:00

abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

journal_title：Journal of biochemistry

authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

更新日期：2009-04-01 00:00:00

abstract：:Studies were performed in the rat liver to examine whether or not insulin as well as glucagon causes the induction of mitochondrial serine:pyruvate aminotransferase (SPTm) [EC 2.6.1.51] and if so, whether the mechanisms of induction are similar or different for the two hormones. Not only glucagon but also insulin indu...

journal_title：Journal of biochemistry

authors： Miyajima H,Oda T,Ichiyama A

更新日期：1989-04-01 00:00:00

abstract：:Silent information regulator type-1 (SIRT1) is crucial during the development of acute respiratory distress syndrome (ARDS). We aimed to explore whether SIRT1 activation could protect against ARDS. SIRT1 was activated by its agonist SRT1720. ARDS was induced by intraperitoneal injection of 5 mg/kg lipopolysaccharide (...

journal_title：Journal of biochemistry

authors： Zhan Y,Yang C,Zhang Q,Yao L

更新日期：2021-01-22 00:00:00

abstract：:Specific and high titer antisera against 4-O-methylglucuronic acid-containing glycosphingolipid (Lipid IV) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits. The antisera were found to agglutinate spermatozoa of three fresh-water bivalves, H. schlegelii, Anodonta woodiana, and C...

journal_title：Journal of biochemistry

authors： Sugita M,Inoue T,Itasaka O,Hori T

更新日期：1984-03-01 00:00:00

abstract：:Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

journal_title：Journal of biochemistry

authors： Asayama M,Kobayashi Y

更新日期：1993-09-01 00:00:00

abstract：:Histidinoalanine, a cross-linking component of connective tissue proteins, was detected in the acid hydrolysate of human urine. The concentrations in urines from newborn babies, children, and adults were 1.33 +/- 0.27, 0.77 +/- 0.23, and 0.89 +/- 0.33 nmol/mg creatinine, respectively. Possible origins of urinary histi...

journal_title：Journal of biochemistry

authors： Fujimoto D

更新日期：1986-09-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

journal_title：Journal of biochemistry

authors： Oda S,Sato M,Toyoshima S,Osawa T

更新日期：1989-06-01 00:00:00

abstract：:Immunoblotting showed that a monoclonal antibody, 3A10, binds to a series of rat brain-specific antigens with molecular masses of 150-, 120-, 118-, 106-, 104-, 79-, and 77-kDa. The expression of 3A10 antigens is dependent on the developmental stage of the brain; only the 106-kDa antigen is detected during embryonic st...

journal_title：Journal of biochemistry

authors： Harada A,Takeuchi K,Dohmae N,Takio K,Uenaka T,Aoki J,Inoue K,Umeda M

更新日期：1999-03-01 00:00:00

abstract：:Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actin. The kinetics of depolymerization suggest that aplyronine A...

journal_title：Journal of biochemistry

authors： Saito S,Watabe S,Ozaki H,Kigoshi H,Yamada K,Fusetani N,Karaki H

更新日期：1996-09-01 00:00:00

abstract：:Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

journal_title：Journal of biochemistry

authors： Mikami B,Nomura K,Morita Y

更新日期：1983-07-01 00:00:00

abstract：:Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin,...

journal_title：Journal of biochemistry

authors： Murayama T,Nakauchi Y,Kimura S,Maruyama K

更新日期：1989-02-01 00:00:00

abstract：:Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

journal_title：Journal of biochemistry

authors： Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

更新日期：2001-04-01 00:00:00