Novel actin depolymerizing macrolide aplyronine A.

abstract：:The Basidiomycete ras gene possesses a pyrimidine-rich stretch (CT-motif) with a short (7 bases) mirror repeat in which its major transcription start point is contained. To analyze the tertiary structure induced by the CT/AG-biased sequence and its effect on gene expression in supercoiled plasmids in Escherichia coli,...

journal_title：Journal of biochemistry

authors： Yamazaki T,Hasebe T,Shouguchi J,Amano H,Kajiwara S,Shishido K

更新日期：1997-10-01 00:00:00

abstract：:Recently, we revealed that microtubule-associated protein (MAP) 4 isoforms, which differ in the number of repeat sequences, alter the microtubule surface properties, and we proposed a hypothesis stating that the change in the surface properties may regulate the movements of microtubule motors [Tokuraku et al. (2003) J...

journal_title：Journal of biochemistry

authors： Tokuraku K,Noguchi TQ,Nishie M,Matsushima K,Kotani S

更新日期：2007-04-01 00:00:00

abstract：:Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1981-09-01 00:00:00

abstract：:Nitric oxide (NO) has diverse effects on immune responses and hepatic functions. In BNL CL.2 cells, the murine embryonic liver cells, inducible nitric oxide synthase (iNOS) mRNA expression appeared after 3 h of treatment with IFN-gamma and LPS. Interestingly, mRNA and protein expression of iNOS was down-regulated by s...

journal_title：Journal of biochemistry

authors： Lee BS,Kim YM,Kang HS,Kim HM,Pyun KH,Choi I

更新日期：2001-01-01 00:00:00

abstract：:The readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunob...

journal_title：Journal of biochemistry

authors： Yesmin F,Bhuiyan RH,Ohmi Y,Ohkawa Y,Tajima O,Okajima T,Furukawa K,Furukawa K

更新日期：2020-08-01 00:00:00

abstract：:Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Tagaya M,Mizushima S

更新日期：1997-02-01 00:00:00

abstract：:Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activating proteins called (re)activases. The X-ray structures of radical enz...

journal_title：Journal of biochemistry

authors： Shibata N,Toraya T

更新日期：2015-10-01 00:00:00

abstract：:Glucuronolactone reductase [EC 1.1.1.20] from rat kidney was purified over 300-fold by ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite columns, and preparative isoelectric focusing. The substrate specificity of the enzyme in the reduction reaction was broad, and hexuronic acid was ...

journal_title：Journal of biochemistry

authors： Hayashi S,Watanabe M,Kimura A

更新日期：1984-01-01 00:00:00

abstract：:The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

journal_title：Journal of biochemistry

authors： Giga Y,Ikai A

更新日期：1985-07-01 00:00:00

abstract：:A cell-wall-associated proteinase from Lactobacillus helveticus CP790, grown in skim milk, was purified to homogeneity by DEAE ion exchange chromatography in the presence of EDTA. Its molecular weight was estimated to be 45,000 by SDS-PAGE and also by the recovery of proteinase activity only from this fraction of SDS-...

journal_title：Journal of biochemistry

authors： Yamamoto N,Akino A,Takano T

更新日期：1993-11-01 00:00:00

abstract：:A cell-free protein-synthesizing system active in initiation of translation of both endogenous mRNA and exogenous mRNA has been obtained from postmitochondrial supernatant (S-12) of the liver of ethionine-treated rats by adding reticulocyte ribosomal extract as a source of initiation factor. Formation of polysomes in ...

journal_title：Journal of biochemistry

authors： Hase M,Endo Y,Natori Y

更新日期：1982-05-01 00:00:00

abstract：:Amyloid beta peptide (Abeta) is a physiological peptide that is constantly catabolized in the brain. We previously demonstrated that an endopeptidase sensitive to phosphoramidon and thiorphan conducts the initial rate-limiting proteolysis of Abeta in vivo, but the exact molecular identity of the peptidase(s) has remai...

journal_title：Journal of biochemistry

authors： Takaki Y,Iwata N,Tsubuki S,Taniguchi S,Toyoshima S,Lu B,Gerard NP,Gerard C,Lee HJ,Shirotani K,Saido TC

更新日期：2000-12-01 00:00:00

abstract：:Using an immunoblotting technique, we have studied the processing of plasma proteins in subcellular fractions of rat liver including rough and smooth microsomes and the Golgi subfractions. Each subcellular fraction was directly subjected to SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting with ant...

journal_title：Journal of biochemistry

authors： Oda K,Miki K,Hirose S,Takami N,Misumi Y,Ikehara Y

更新日期：1986-12-01 00:00:00

abstract：:1) A method is described for the separation and fractionation of nonhistone chromosomal proteins from salt-urea dissociated calf thymus chromatin. After precipitating DNA in the dissociated chromatin solution with LaCl3, the chromosomal proteins in the supernatant were fractionated by SP-Sephadex C-25 column chromatog...

journal_title：Journal of biochemistry

authors： Yoshida M,Kikuchi A,Shimura K

更新日期：1975-05-01 00:00:00

abstract：:Histidine and its derivatives increased rabbit muscle fructose 1,6-bisphosphatase activity at neutral pH with positive cooperativity. In the presence of histidine and carnosine the optimum pH shifted from pH 8.0 to 7.4. The cooperative response of the enzyme to AMP and fructose 1,6-bisphosphate was observed in the pre...

journal_title：Journal of biochemistry

authors： Ikeda T,Kimura K,Hama T,Tamaki N

更新日期：1980-01-01 00:00:00

abstract：:Binding of native connectin (2,100 kDa fragment of alpha-connectin) to myosin filaments was investigated using a sedimentation technique and densitometric estimations of the separated proteins. In the presence of 60 mM KCl and 5 mM phosphate buffer, pH 7.0, as much as 1.5 mol of connectin was bound to 1 mol of myosin,...

journal_title：Journal of biochemistry

authors： Murayama T,Nakauchi Y,Kimura S,Maruyama K

更新日期：1989-02-01 00:00:00

abstract：:Matriptase is a type II transmembrane serine protease expressed abundantly in the epithelial cells and keratinocyte. It plays a key role in the establishment and maintenance of epithelial integrity. Matriptase is considered to be at the most upstream in cellular protease cascade. Activation of its zymogen is the most ...

journal_title：Journal of biochemistry

authors： Kojima K,Inouye K

更新日期：2011-08-01 00:00:00

abstract：:The denaturation of lysozyme and ribonuclease A by guanidine hydrochloride was followed in the presence and absence of glycerol and sorbitol by means of circular dichroism measurements at 25 degrees C. The protein-solvent interactions in the presence of these polyols were also studied by means of density measurements,...

journal_title：Journal of biochemistry

authors： Gekko K,Ito H

更新日期：1990-04-01 00:00:00

abstract：:Mutations of epsilon-sarcoglycan gene (SGCE) have been implicated in myoclonus-dystonia (M-D), a movement disorder. To determine the pathophysiology of M-D, we produced Sgce knockout mice and found that the knockout mice exhibited myoclonus, motor impairments, hyperactivity, anxiety, depression, significantly higher l...

journal_title：Journal of biochemistry

authors： Yokoi F,Dang MT,Li J,Li Y

更新日期：2006-07-01 00:00:00

abstract：:The traditional two-step EGTA/collagenase method is widely used in studying nitric oxide (NO) production in hepatocytes. The present study first revealed that hepatocytes isolated by this method spontaneously express an iNOS mRNA. Thereafter, based on this novel finding, we characterized the expression and regulation ...

journal_title：Journal of biochemistry

authors： Wang H,Gao X,Fukumoto S,Tademoto S,Sato K,Hirai K

更新日期：1998-11-01 00:00:00

abstract：:Spo0A is both a positive and a negative transcriptional regulator which plays a very important role in sporulation initiation in Bacillus subtilis. Its N-terminal amino acid sequence is homologous to that of regulator proteins of the two-component regulatory systems involved in signal transduction in bacteria. Phospho...

journal_title：Journal of biochemistry

authors： Asayama M,Kobayashi Y

更新日期：1993-09-01 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:Alanine-glyoxylate aminotransferase (AGT) 2 is a pyridoxal 5'-phosphate dependent, mitochondrial enzyme which, in the rat, is expressed at a high level in the kidney. The amino acid sequences of nine tryptic and seven CNBr peptides of the rat kidney AGT2 were determined. Three overlapping cDNAs encoding the AGT2 were ...

journal_title：Journal of biochemistry

authors： Lee IS,Muragaki Y,Ideguchi T,Hase T,Tsuji M,Ooshima A,Okuno E,Kido R

更新日期：1995-04-01 00:00:00

abstract：:The plant hormone ethylene is generated from a unique precursor, 1-aminocyclopropane-1-carboxylate (ACC). In previous studies, ACC deaminase, which degrades ACC to alpha-ketobutyrate and ammonia, was found in four strains of Pseudomonas, characterized, and sequenced. To verify the wider distribution of ACC deaminase i...

journal_title：Journal of biochemistry

authors： Minami R,Uchiyama K,Murakami T,Kawai J,Mikami K,Yamada T,Yokoi D,Ito H,Matsui H,Honma M

更新日期：1998-06-01 00:00:00

abstract：:The oleate (delta 9-18:1)/cis-vaccenate (delta 11-18:1) ratios in phospholipids increased in the order of normal liver, host liver and hepatoma in rats. The amount of oleate increased in phospholipids and decreased in triacylglycerol in the same order, whereas the distributions of cis-vaccenate among the major lipid c...

journal_title：Journal of biochemistry

authors： Hori T,Okuyama H

更新日期：1987-05-01 00:00:00

abstract：:The interaction between riboflavin and riboflavin binding protein (RBP) was studied by 13C-NMR spectroscopy. The 13C-NMR spectra of riboflavin selectively enriched at the 2-, 4-, 4a-, and 10a-positions and of (3-[13C]methyl)riboflavin were measured both in the free and RBP-bound forms. The 13C signals of 13C-enriched ...

journal_title：Journal of biochemistry

authors： Miura R,Tojo H,Fujii S,Yamano T,Miyake Y

更新日期：1984-07-01 00:00:00

abstract：:The position in the acyl phosphate linkage of the phosphorylated intermediate of (Na+, K+)-ATPase that is cleaved by N-methylhydroxylamine was compared with that of the model compound acetylphosphate. The products of the cleavage of the phosphoenzyme by methylhydroxylamine were the active enzyme and a N-P compound, no...

journal_title：Journal of biochemistry

authors： Ushimaru M,Shinohara Y,Fukushima Y

更新日期：1997-09-01 00:00:00

abstract：:Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however,...

journal_title：Journal of biochemistry

authors： Yamazawa R,Nakajima Y,Mushiake K,Yoshimoto T,Ito K

更新日期：2011-06-01 00:00:00

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:Mitochondria exhibiting cyanide-resistant respiration were isolated from Hansenula anomala which had been incubated in the presence of antimycin A to induce cyanide-resistant respiration. The cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin A or myxothiazol, suggesting that the bra...

journal_title：Journal of biochemistry

authors： Sakajo S,Minagawa N,Komiyama T,Yoshimoto A

更新日期：1990-08-01 00:00:00