Crystal structure of serine dehydrogenase from Escherichia coli: important role of the C-terminal region for closed-complex formation.

abstract：:Manganese ion, like Mg2+, has been found to produce high biosynthetic activity of the unadenylylated form of glutamine synthetase obtained from Mycobacterium smegmatis, and the activity with each of these cations was decreased by the adenylylation of the enzyme. Further, the gamma-glutamyltransferase reaction was cata...

journal_title：Journal of biochemistry

authors： Matsuoka K,Kimura K

更新日期：1985-04-01 00:00:00

abstract：:Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...

journal_title：Journal of biochemistry

authors： Furuichi H,Ozaki Y,Niki K,Akutsu H

更新日期：1990-11-01 00:00:00

abstract：:Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

journal_title：Journal of biochemistry

authors： Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

更新日期：1981-05-01 00:00:00

abstract：:Antizyme (AZ) interacts with ornithine decarboxylase, which catalyzes the first step of polyamine biosynthesis and recruits it to the proteasome for degradation. Synthesizing the functional AZ protein requires transition of the reading frame at the termination codon. This programmed +1 ribosomal frameshifting is induc...

journal_title：Journal of biochemistry

authors： Oguro A,Shigeta T,Machida K,Suzuki T,Iwamoto T,Matsufuji S,Imataka H

更新日期：2020-08-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:Detergent treatments were examined for their efficacy in purifying trimethylamine-N-oxide (TMAO) demethylase activity from fish muscle microsomes. Tritons X-100 and X-45, deoxycholate, Brijs, Tweens 20, 65, and 80, and SDS were generally ineffective in solubilizing demethylase activity from this membrane fraction, at ...

journal_title：Journal of biochemistry

authors： Parkin KL,Hultin HO

更新日期：1986-07-01 00:00:00

abstract：:Substrate specificities of phospholipases C[EC 3.1.4.3] from Clostridium novyi, Clostridium perfringens, Bacillus cereus, and Pseudomonas aureofaciens were studied under the same conditions. Phospholipases C from Clostridium novyi and Bacillus cereus show wide substrate specificities while those of Clostridium perfrin...

journal_title：Journal of biochemistry

authors： Taguchi R,Ikezawa H

更新日期：1977-11-01 00:00:00

abstract：:Equilibrium gel permeation chromatography was employed to determine the ability of heparin to form complexes with thrombin and antithrombin III. In the eluate from a Sephacryl S-200 column, heparin caused a peak and then a trough in the fluorescence of 48 nM antithrombin III or 63 nM thrombin. The peak-heights with kn...

journal_title：Journal of biochemistry

authors： Oshima G,Uchiyama H,Nagasawa K

更新日期：1984-10-01 00:00:00

abstract：:High mobility group (HMG) protein 1 contains two DNA binding motifs, called HMG1/2-boxes, linked with a linker region. The functional relationships between the two boxes and the mechanism of involvement of the linker region for effective binding of HMG1 were examined. The binding analyses of truncated HMG1 peptides wi...

journal_title：Journal of biochemistry

authors： Saito K,Kikuchi T,Shirakawa H,Yoshida M

更新日期：1999-02-01 00:00:00

abstract：:The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

journal_title：Journal of biochemistry

authors： Takenaka H,Ikehara M,Tonomura Y

更新日期：1976-12-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:The mitogen-activated protein (MAP) kinase cascades play essential roles in a variety of cell processes by influencing transcriptional or translational regulation. ERKs play a central role in survival and mitogenic signaling, while JNKs and p38 MAP kinases are preferentially activated by environmental stresses and are...

journal_title：Journal of biochemistry

authors： Matsukawa J,Matsuzawa A,Takeda K,Ichijo H

更新日期：2004-09-01 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four ...

journal_title：Journal of biochemistry

authors： Hirose J,Sakurai T,Imamura K,Watanabe H,Iwamoto H,Hiromi K,Itoh H,Shin T,Murao S

更新日期：1994-05-01 00:00:00

abstract：:The absorption and fluorometric changes of the reaction mixture of luciferase-FMNH2 complex with O2 were re-examined. Rapid formation (k2(app) = 2.0 s-1 at [O2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than o...

journal_title：Journal of biochemistry

authors： Ashizawa N,Nakamura T,Watanabe T

更新日期：1977-04-01 00:00:00

abstract：:In the nucleus of eukaryotic cells, chromatin is tethered to the nuclear envelope (NE), wherein inner nuclear membrane proteins (INMPs) play major roles. However, in Xenopus blastula, chromatin tethering to the NE depends on nuclear filamentous actin that develops in a blastula-specific manner. To investigate whether ...

journal_title：Journal of biochemistry

authors： Oda H,Kato S,Ohsumi K,Iwabuchi M

更新日期：2020-11-10 00:00:00

abstract：:The flagellar motor is composed of the stator and the rotor, and the interaction between the stator and the rotor at the cytoplasmic region is believed to produce mechanical force for the rotation of flagella. The periplasmic region of the stator has been proposed to play an important role in assembly around and incor...

journal_title：Journal of biochemistry

authors： Hizukuri Y,Kojima S,Homma M

更新日期：2010-09-01 00:00:00

abstract：:Reaction center of chromatophores of Rhodospirillum rubrum consists of three kinds of protein, H-, M-, and L-subunit, and is bound with many other kinds of protein to form a larger protein complex (PRU; photoreaction unit), which contains all the bacteriochlorophyll. In the present study, purified PRU was dissociated ...

journal_title：Journal of biochemistry

authors： Tanaka K,Kakuno T,Yamashita J,Horio T

更新日期：1983-12-01 00:00:00

abstract：:Polynucleotide phosphorylase from Thermus thermophilus catalyzed the addition of short guanylyl blocks from GDP to the 3'-hydroxyl termini of oligonucleotide primers at low temperature in a simple reaction mixture. Polyguanylic acid formation was inhibited at 37 degrees C, but the addition of one or two guanylyl resid...

journal_title：Journal of biochemistry

authors： Kikuchi Y,Hirai K,Sakaguchi K

更新日期：1979-11-01 00:00:00

abstract：:A simple, sensitive and reliable method for the detection of cystine-containing peptides has been developed. A peptide bridged with a disulfide bond was reduced and cleaved with tributylphosphine, and then coupled with a thiol specific reagent, ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate, under alkaline condi...

journal_title：Journal of biochemistry

authors： Sueyoshi T,Miyata T,Iwanaga S,Toyo'oka T,Imai K

更新日期：1985-06-01 00:00:00

abstract：:The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

journal_title：Journal of biochemistry

authors： Morita T,Huruta T,Ashiuchi M,Yagi T

更新日期：2002-10-01 00:00:00

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

journal_title：Journal of biochemistry

authors： Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

更新日期：1987-08-01 00:00:00

abstract：:Polychlorinated biphenyls (PCBs) are well-known recalcitrant environmental pollutants. Although the metabolism of the PCBs has been intensively studied, very little is known about their mechanism of toxicity in living organisms or how they are degraded. We have examined the effects of PCBs on two different yeast strai...

journal_title：Journal of biochemistry

authors： Ryu JH,Lee Y,Han SK,Kim HY

更新日期：2003-07-01 00:00:00

abstract：:Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and ...

journal_title：Journal of biochemistry

authors： Hanzawa H,Haruyama H,Konishi K,Watanabe K,Tsurufuji S

更新日期：1998-01-01 00:00:00

abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

journal_title：Journal of biochemistry

authors： Ohe Y,Hayashi H,Iwai K

更新日期：1979-02-01 00:00:00

abstract：:Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from lambda gt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without th...

journal_title：Journal of biochemistry

authors： Zhang R,Yatsuki H,Kusakabe T,Iwabe N,Miyata T,Imai T,Yoshida M,Hori K

更新日期：1995-03-01 00:00:00

abstract：:A large COOH-terminal domain of Serratia marcescens serine protease (SSP) is essentially required for the excretion of the mature protease region through the outer membrane in Escherichia coli. For investigation of the possibility of transporting foreign periplasmic proteins by utilizing the function of the COOH-termi...

journal_title：Journal of biochemistry

authors： Shimada K,Ohnishi Y,Horinouchi S,Beppu T

更新日期：1994-08-01 00:00:00

abstract：:A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono Q. The enzyme had a...

journal_title：Journal of biochemistry

authors： Ohmoto T,Kinoshita T,Moriyoshi K,Sakai K,Hamada N,Ohe T

更新日期：1998-09-01 00:00:00

abstract：:Pollen proteins from several grass species have been identified and characterized as causative allergens in grass pollinosis. In contrast, allergenic potential of pollen proteins from rice, which belongs to the same Poaceae family, has not well been investigated, despite that a few clinical cases have been reported on...

journal_title：Journal of biochemistry

authors： Hirano K,Hino S,Oshima K,Okajima T,Nadano D,Urisu A,Takaiwa F,Matsuda T

更新日期：2013-08-01 00:00:00