Abstract:
:A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono Q. The enzyme had a molecular mass of 25 and 27 kDa as estimated by SDS-PAGE and Superdex 200, respectively. Its N-terminal 30 amino acid sequence had high homology with the deduced amino acid sequences of the 2HC2CA isomerase of nahD (a gene of naphthalene metabolism), pahD (a gene of naphthalene and phenanthrene metabolism), and doxJ (a gene of dibenzothiophene metabolism). The enzymatic product was a trans isomer. The isomerase activity was inhibited in the presence of monoiodoacetate or Hg2+, but not by preincubation with monoiodoacetate or N-ethylmaleimide. GSH functioned as a cofactor and activated the enzyme at above 0.15 mM.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Ohmoto T,Kinoshita T,Moriyoshi K,Sakai K,Hamada N,Ohe Tdoi
10.1093/oxfordjournals.jbchem.a022152subject
Has Abstractpub_date
1998-09-01 00:00:00pages
591-7issue
3eissn
0021-924Xissn
1756-2651journal_volume
124pub_type
杂志文章abstract::When a rat hepatoma cell (R-Y121B) homogenate was incubated at 37 degrees C, 30-70% of the total alkaline phosphatase was released into the supernatant fluid from the precipitate fractions. The release reached a plateau level after 10 h of incubation at 37 degrees C. The optimum pH value for the release was 7.4. Alkal...
journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134476
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123982
更新日期:1992-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123901
更新日期:1992-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132080
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pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
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更新日期:2012-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133620
更新日期:1981-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021857
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133045
更新日期:1980-09-01 00:00:00
abstract::A real time biomolecular interaction assay system involving an optical sensor was applied to the quantitative analysis of the binding of herpes simplex virus type 1 (HSV-1) to Vero cells, and the neutralization antibody titer against this virus with a commercially available sulfonated human immunoglobulin preparation....
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doi:10.1093/oxfordjournals.jbchem.a021632
更新日期:1997-04-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135050
更新日期:1985-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130916
更新日期:1975-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003198
更新日期:2002-07-01 00:00:00
abstract::The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...
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pub_type: 杂志文章,评审
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更新日期:2010-01-01 00:00:00