Isolation of a membrane protein from R rubrum chromatophores and its abnormal behavior in SDS-polyacrylamide gel electrophoresis due to a high binding capacity for SDS.

abstract：:An ultraviolet absorption difference spectrum characteristic of the ionization change of a tyrosyl residue was observed on the binding of subtilisin BPN' with Streptomyces subtilisin inhibitor (SSI) at alkaline pH. This difference spectrum was considered to be induced by a pKa shift (from 9.7 to > or = 11.5) of a tyro...

journal_title：Journal of biochemistry

authors： Masuda-Momma K,Shimakawa T,Inouye K,Hiromi K,Kojima S,Kumagai I,Miura K,Tonomura B

更新日期：1993-12-01 00:00:00

abstract：:Porcine adrenocortical lysosomes were characterized by differential centrifugation, acid hydrolase contents, latency of cathepsin D, release of bound acid hydrolases in soluble form, and isopycnic density gradient centrifugation. Cathepsins D and B, beta-N-acetylglucosaminidase, beta-galactosidase and arylsulphatase w...

journal_title：Journal of biochemistry

authors： Taguchi S,Watabe S,Kouyama H,Tatsunami S,Yago N

更新日期：1981-05-01 00:00:00

abstract：:The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the...

journal_title：Journal of biochemistry

authors： Yamamoto S,Okujo N,Yoshida T,Matsuura S,Shinoda S

更新日期：1994-05-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Three alkaline DNases, A, B, and C, with preference for the digestion of double-stranded DNA (dsDNA) were partially purified from microplasmodia of Physarum polycephalum. They were very similar but differed in their isoelectric points. These were pH 5.8 for DNase A, 7.1 for DNase B, and 9.1 for DNase C. All three enzy...

journal_title：Journal of biochemistry

authors： Waterborg JH,Kuyper CM

更新日期：1980-02-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:PKNalpha is a fatty acid- and Rho-activated serine/threonine protein kinase having a catalytic domain homologous to members of the protein kinase C family. Recently it was reported that PKNalpha is involved in the p38 mitogen-activated protein kinase (MAPK) signaling pathway. To date, however, how PKNalpha regulates t...

journal_title：Journal of biochemistry

authors： Takahashi M,Gotoh Y,Isagawa T,Nishimura T,Goyama E,Kim HS,Mukai H,Ono Y

更新日期：2003-02-01 00:00:00

abstract：:A hybridoma cell line producing mouse monoclonal antibody against pig kidney Na,K-ATPase was established. The antibody, named 38 (mAb38, IgG1), was purified from mouse ascites fluid by chromatography on a protein A-Sepharose column. Antigens immobilized on microplate wells with p-benzoquinone were used for titer assay...

journal_title：Journal of biochemistry

authors： Urayama O,Nagamune H,Nakao M,Hara Y,Sugiyama H,Sato K,Nakao T

更新日期：1985-07-01 00:00:00

abstract：:To better understand the regulation of ryudocan (syndecan-4) expression, we have determined the structural organization of the human ryudocan gene. The human ryudocan gene extends approximately 24 kilobases and is divided into five exons, which appear to be conserved in syndecan family members. Exon I encodes the sign...

journal_title：Journal of biochemistry

authors： Takagi A,Kojima T,Tsuzuki S,Katsumi A,Yamazaki T,Sugiura I,Hamaguchi M,Saito H

更新日期：1996-05-01 00:00:00

abstract：:After screening 900 E. coli strains of the Clarke and Carbon collection for by lysophospholipase L1 activities, we isolated a clone bearing the plasmid pLC6-34, which showed an increased level of lysophospholipase L1 activity. Strains bearing the plasmid pC124, a subclone of pLC6-34 in plasmid vector pUC8, showed appr...

journal_title：Journal of biochemistry

authors： Karasawa K,Kudo I,Kobayashi T,Homma H,Chiba N,Mizushima H,Inoue K,Nojima S

更新日期：1991-02-01 00:00:00

abstract：:We have purified L-kynurenine 3-monooxygenase from pig liver mitochondria using a procedure involving seven steps composed of (1) preparation of mitochondrial outer membrane, (2) preparation of the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) insoluble outer membrane mater...

journal_title：Journal of biochemistry

authors： Uemura T,Hirai K

更新日期：1998-02-01 00:00:00

abstract：:Three forms of cathepsin H-like cysteine proteinase were purified from rat spleen by a method involving acid treatment and chromatography on pepstatin-Sepharose, Sephadex G-75, DEAE-Sephacel, CM-Toyopearl, and concanavalin A-Sepharose. The final preparations of these forms all migrated as single protein bands on polya...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Kato Y

更新日期：1984-02-01 00:00:00

abstract：:The conditions and pretreatments required in the conventional pyridine hemochrome method were re-examined for application of the method to the measurement of protoheme and heme c contents in photosynthetically grown cells of Rhodopseudomonas sphaeroides. The amounts of hemes were calculated from two kinds of differenc...

journal_title：Journal of biochemistry

authors： Takaichi S,Morita S

更新日期：1981-05-01 00:00:00

abstract：:ADAMTS13 is the metalloprotease responsible for the proteolytic degradation of von Willebrand factor (VWF). A severe deficiency of this VWF-cleaving protease activity causes thrombotic thrombocytopenic purpura. This protease, comprising 1,427 amino acid residues, is composed of multiple domains, i.e., a preproregion, ...

journal_title：Journal of biochemistry

authors： Soejima K,Nakamura H,Hirashima M,Morikawa W,Nozaki C,Nakagaki T

更新日期：2006-01-01 00:00:00

abstract：:Cholesterol: oxygen oxidoreductase [EC 1.1.3.6] from Brevibacterium sterolicum (ATCC 21387) was found to catalyze the oxidation of steroids such as sterols, steroid hormones, and bile acids having a free C-3beta hydroxyl group. However, the enzyme was inactive towards estradiol and estriol and had a weak activity towa...

journal_title：Journal of biochemistry

authors： Ikawa S,Takita M,Ogura M

更新日期：1979-06-01 00:00:00

abstract：:Screening of four malic enzymes--NAD-linked enzyme [EC 1.1.1.38], NAD, NADP-linked enzyme [EC 1.1.1.39], NADP-linked enzyme [EC 1.1.1.40], and D-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of Sepharose 6B column chromatography: 9 strains of enteric bacteria,...

journal_title：Journal of biochemistry

authors： Iwakura M,Tokushige M,Katsuki H,Muramatsu S

更新日期：1978-05-01 00:00:00

abstract：:It is not known whether the activity of the Escherichia coli protein export system changes during the division cycle. To address and answer this question, we took two approaches. First, we pulse-labeled a random culture and size-fractionated the labeled cells in the presence of inhibitors of secretion. Second, we puls...

journal_title：Journal of biochemistry

authors： Taura T,Ito K

更新日期：1991-06-01 00:00:00

abstract：:Low molecular weight RNAs were isolated from nuclei of the cellular slime mold Dictyostelium discoideum AX-3. Analysis of the RNAs by polyacrylamide gel electrophoresis showed that the vegetative cell nuclei contained, besides tRNA (Dd1), 5S RNA (Dd4), and 5.8S RNA (Dd7), at least 7 small RNA species (Dd3, Dd5, Dd6, D...

journal_title：Journal of biochemistry

authors： Takeishi K,Kaneda S

更新日期：1981-08-01 00:00:00

abstract：:Human matrix metalloproteinase 7 (MMP-7) activity exhibits broad bell-shaped pH profile with the acidic and alkaline pK(a) (pK(e1) and pK(e2)) values of about 4 and 10. The ionizable group for pK(e2) was assigned to Lys or Arg by thermodynamic analysis; however, no such residues are present in the active site. Hence, ...

journal_title：Journal of biochemistry

authors： Morishima A,Yasukawa K,Inouye K

更新日期：2012-05-01 00:00:00

abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

journal_title：Journal of biochemistry

authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

更新日期：2009-04-01 00:00:00

abstract：:The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (Mr 55,830). The predicted amino acid sequence was consistent with the partial a...

journal_title：Journal of biochemistry

authors： Xu XM,Koyama N,Cui M,Yamagishi A,Nosoh Y,Oshima T

更新日期：1991-05-01 00:00:00

abstract：:Caldesmon, calmodulin-, and actin-binding protein of chicken gizzard did not affect the process of polymerization of actin induced by 0.1 M KCl. Caldesmon binds to F-actin, thus inhibiting the gelation action of actin binding protein (ABP; filamin). Low shear viscosity and flow birefringence measurements revealed that...

journal_title：Journal of biochemistry

authors： Maruyama K,Morimoto K,Kijima Y,Sobue K,Kakiuchi S

更新日期：1985-05-01 00:00:00

abstract：:Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

journal_title：Journal of biochemistry

authors： Nakanaga K,Hama K,Aoki J

更新日期：2010-07-01 00:00:00

abstract：:Urate oxidases (UOs) catalyze the cofactor-independent oxidation of uric acid, and an extensive water network in the active site has been suggested to play an essential role in the catalysis. For our present analysis of the structure and function of the water network, the crystal qualities of Bacillus sp. TB-90 urate ...

journal_title：Journal of biochemistry

authors： Hibi T,Itoh T

更新日期：2020-10-01 00:00:00

abstract：:Many membrane proteins such as ion channels are oligomers, but the determinants of the degree of oligomerization are not fully understood. Mechanosensitive channel with large conductance (MscL), which is ubiquitous in bacteria, is a homopentamer with two transmembrane helices and a cytoplasmic helix in each subunit. T...

journal_title：Journal of biochemistry

authors： Ando C,Liu N,Yoshimura K

更新日期：2015-08-01 00:00:00

abstract：:Upregulation and nuclear retention of the human histone demethylase LSD1 are correlated with aggressiveness and poor outcome of several cancer types, but the molecular mechanism of LSD1 nuclear import remains unclear. Here, we found that the N-terminal flexible region of LSD1 contains a nuclear localization signal (NL...

journal_title：Journal of biochemistry

authors： Jin Y,Kim TY,Kim MS,Kim MA,Park SH,Jang YK

更新日期：2014-12-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:The substrate specificity of microbial transglutaminase (MTG) from Streptomyces mobaraensis (formerly categorized Streptoverticillium) was studied using a Streptomyces proteinaceous protease inhibitor, STI2, as a model amine-donor substrate. Chemical modification and mutational analysis to address the substrate requir...

journal_title：Journal of biochemistry

authors： Taguchi S,Nishihama KI,Igi K,Ito K,Taira H,Motoki M,Momose H

更新日期：2000-09-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:Proteolytic trimming of isoprenylated proteins, including Ras, at the C-terminal CAAX motifs is a key event in their activation. However, the protease responsible for the proteolysis has not been well characterized yet. In this study, we established a novel assay method for the enzyme using a fluorescent substrate, da...

journal_title：Journal of biochemistry

authors： Nishii W,Muramatsu T,Kuchino Y,Yokoyama S,Takahashi K

更新日期：1997-08-01 00:00:00