Chicken ornithine transcarbamylase: purification and some properties.

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:The effects of intra- and extravesicular calcium and magnesium ions on the hydrolysis of the phosphoenzyme (EP) intermediate formed in the reaction of Ca2+,Mg2+-dependent ATPase of the sarcoplasmic reticulum were investigated. The rate constants of EP hydrolysis were measured under conditions that allowed a single tur...

journal_title：Journal of biochemistry

authors： Daiho T,Takisawa H,Yamamoto T

更新日期：1985-02-01 00:00:00

abstract：:This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged base-denatured ferricytochrome c (UB-state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of...

journal_title：Journal of biochemistry

authors： Jain R,Sharma D,Kumar R,Kumar R

更新日期：2019-02-01 00:00:00

abstract：:d-Aspartate is found in the nervous and reproductive system and participates in various physiological roles. While several lines of evidence suggest that this amino acid has an endogenous origin, the enzyme responsible for mammalian d-Asp biosynthesis has not yet been identified. We show that mammalian serine racemase...

journal_title：Journal of biochemistry

authors： Ito T,Hayashida M,Kobayashi S,Muto N,Hayashi A,Yoshimura T,Mori H

更新日期：2016-12-01 00:00:00

abstract：:HPC-1/syntaxin 1A (HPC-1), which plays an important role in vesicular transport to the plasma membrane, possesses a hydrophobic sequence at its C terminus. When expressed from cDNA in COS cells, wild-type HPC-1 was localized in the Golgi complex and the plasma membrane. Truncation of the hydrophobic domain resulted in...

journal_title：Journal of biochemistry

authors： Masaki R,Yamamoto A,Akagawa K,Tashiro Y

更新日期：1998-08-01 00:00:00

abstract：:An escape mutant of human parainfluenza virus type 1 (hPIV1), which was selected by serial passage in the presence of a sialidase inhibitor, 4-O-thiocarbamoylmethyl-2-deoxy-2,3-didehydro-N-acetylneur-aminic acid (TCM-Neu5Ac2en), exhibited remarkable syncytium formation and virus-induced cell death in LLC-MK2 cells but...

journal_title：Journal of biochemistry

authors： Takaguchi M,Takahashi T,Hosokawa C,Ueyama H,Fukushima K,Hayakawa T,Itoh K,Ikeda K,Suzuki T

更新日期：2011-02-01 00:00:00

abstract：:A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activi...

journal_title：Journal of biochemistry

authors： Maeshima M,Asahi T

更新日期：1981-08-01 00:00:00

abstract：:Phosphoenolpyruvate (PEP) carboxylase [EC 4.1.1.31] of E. coli was inactivated by 2,4,6-trinitrobenzene sulfonate (TNBS), a reagent known to attack amino groups in polypeptides. When the modified enzyme was hydrolyzed with acid, epsilon-trinitrophenyl lysine (TNP-lysine) was identified as a product. Close similarity o...

journal_title：Journal of biochemistry

authors： Naide A,Izui K,Yoshinaga T,Katsuki H

更新日期：1979-02-01 00:00:00

abstract：:Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its cytosolically exposed domain, is expressed in Saccharomyces cerevisiae. Using various KAR2 promoter constructs in front of ...

journal_title：Journal of biochemistry

authors： Zimmer T,Ogura A,Ohta A,Takagi M

更新日期：1999-12-01 00:00:00

abstract：:The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP we...

journal_title：Journal of biochemistry

authors： Blondet A,Doghman M,Rached M,Durand P,Bégeot M,Naville D

更新日期：2004-04-01 00:00:00

abstract：:Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogeni...

journal_title：Journal of biochemistry

authors： Sakaue T,Maekawa M,Nakayama H,Higashiyama S

更新日期：2017-10-01 00:00:00

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:Mammalian/mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase that responds to environmental determinants such as growth factor concentration, nutrient availability, energy sufficiency and stress. mTOR forms two different complexes, called mTOR complex 1 (mTORC1) and mTORC2, and these ...

journal_title：Journal of biochemistry

authors： Hoshii T,Matsuda S,Hirao A

更新日期：2014-08-01 00:00:00

abstract：:Monoclonal antibodies were raised against a synthetic peptide (43 amino acid residues) that corresponds to the complete profragment of human prorenin. Seven monoclonal antibodies were chosen for further characterization. Two antibodies, 2-X-C1 and 4-X-E1, reacted with the middle region and C-terminus of the profragmen...

journal_title：Journal of biochemistry

authors： Ishizuka Y,Iizumi S,Akiyama F,Hori H,Poorman RA,Lobl TJ,Murakami K

更新日期：1989-09-01 00:00:00

abstract：:The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

journal_title：Journal of biochemistry

authors： Nakajima Y,Nakamura K

更新日期：1991-02-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:Thymosin β4 (Tβ4) is a major intracellular G-actin-sequestering peptide. There is increasing evidence to support important extracellular functions of Tβ4 related to angiogenesis, wound healing and cardiovascular regeneration. We investigated the expression of 'Tβ4' and 'thymosin β10', a closely related peptide, during...

journal_title：Journal of biochemistry

authors： Tokura Y,Nakayama Y,Fukada S,Nara N,Yamamoto H,Matsuda R,Hara T

更新日期：2011-01-01 00:00:00

abstract：:Proteoheteroglycan (PHG) was prepared from Neurospora crassa cells by extraction with hot water followed by cetyltrimethylammoniumbromide fractionation. The polymer was purified by DEAE-cellulose chromatography followed by gel filtrations. The PHG was fractionated into five subfractions containing carbohydrate (65-88%...

journal_title：Journal of biochemistry

authors： Nakajima T,Yoshida M,Hiura N,Matsuda K

更新日期：1984-10-01 00:00:00

abstract：:Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

journal_title：Journal of biochemistry

authors： Seki-Chiba S,Ishimoto M

更新日期：1977-12-01 00:00:00

abstract：:An open reading frame of 828 base pairs was found in the CHO1 gene region of Saccharomyces cerevisiae by nucleotide sequencing analysis. Its enhanced expression with the aid of the PHO5 regulatory sequence resulted in an overproduction of a protein with a molecular weight of approximately 30,000, which in turn was con...

journal_title：Journal of biochemistry

authors： Kiyono K,Miura K,Kushima Y,Hikiji T,Fukushima M,Shibuya I,Ohta A

更新日期：1987-11-01 00:00:00

abstract：:Steroid monooxygenase of Rhodococcus rhodochrous is a Baeyer-Villigerase catalyzing the insertion of an oxygen atom between the C(17)- and C(20)-carbons of progesterone to produce testosterone acetate. The 5.1-kbp-long BamHI DNA fragment containing the steroid monooxygenase gene, smo, was cloned from the chromosomal D...

journal_title：Journal of biochemistry

authors： Morii S,Sawamoto S,Yamauchi Y,Miyamoto M,Iwami M,Itagaki E

更新日期：1999-09-01 00:00:00

abstract：:The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed step in the biosynthesis of aromatic amino acids and vitamins. Through target-directed mutagenesis of the cloned aroG on a p...

journal_title：Journal of biochemistry

authors： Ger YM,Chen SL,Chiang HJ,Shiuan D

更新日期：1994-11-01 00:00:00

abstract：:In the crude extract of matured eggs of the silkworm (Bombyx mori), proteolytic activity was detected only in the acidic pH region with maximal activity at pH 3.5, and this activity was maintained throughout the development. No appreciable activity was observed in the neutral to alkaline pH region either in matured eg...

journal_title：Journal of biochemistry

authors： Kageyama T,Takahashi SY,Takahashi K

更新日期：1981-09-01 00:00:00

abstract：:Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

journal_title：Journal of biochemistry

authors： Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

更新日期：1989-11-01 00:00:00

abstract：:We previously reported the utilization of antisense RNA in the development of a novel immune system against RNA coliphage SP proliferation (Hirashima et al. [1986] Proc. Natl. Acad. Sci. U.S. 83, 7726-7730). We attempted to determine the most effective (i.e., those eliciting antiviral activity) sequences for targeting...

journal_title：Journal of biochemistry

authors： Hirashima A,Sawaki S,Mizuno T,Houba-Herin N,Inouye M

更新日期：1989-07-01 00:00:00

abstract：:Rat liver rhodanese [EC 2.8.1.1] purified by ammonium sulfate fractionation, CM-cellulose and Sephadex G-200 chromatography yielded two active fractions (I & II). Their molecular weights were estimated to be 1.75 X 10(4) (I) and 1.26 X 10(4) (II) by the gel filtration method. Kinetic studies revealed that Fraction I r...

journal_title：Journal of biochemistry

authors： Oi S

更新日期：1975-10-01 00:00:00

abstract：:A rapid micro-assay method for gelatinolytic activitiy has been developed using 3H-labeled heat-denatured polymeric collagen (gelatin) as a substrate to investigate enzymes involved in the post-collagenase catabolism of collagen. The method is based on the incubation of gelatin with enzyme followed by determination of...

journal_title：Journal of biochemistry

authors： Sunada H,Nagai Y

更新日期：1980-06-01 00:00:00

abstract：:The nucleotide sequence of IStaqTZ2 are available in the DDBJ/EMBL/GenBank databases under the accession number AB063392. A novel insertion sequence (IStaqTZ2) was transposed from the genome of Thermus thermophilus TZ2 to that of the thermophilic bacteriophage IN93. The complete nucleotide sequence of IStaqTZ2 was det...

journal_title：Journal of biochemistry

authors： Matsushita I,Yanase H

更新日期：2009-12-01 00:00:00

abstract：:A DNA polymerase beta (pol. beta) inhibitor has been isolated independently from two organisms; a red perilla, Perilla frutescens, and a mugwort, Artemisia vulgaris. These molecules were determined by spectroscopic analyses to be the cyanogenic glucoside, D-mandelonitrile-beta-D-glucoside, prunasin. The compound inhib...

journal_title：Journal of biochemistry

authors： Mizushina Y,Takahashi N,Ogawa A,Tsurugaya K,Koshino H,Takemura M,Yoshida S,Matsukage A,Sugawara F,Sakaguchi K

更新日期：1999-08-01 00:00:00

abstract：:Human HsRad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. We have characterized the self-assembly of HsRad51 by measuring the fluorescence resonance energy transfer from the fluorescein-labeled protein to the rhodamine-labeled protein. Self-as...

journal_title：Journal of biochemistry

authors： Yoshioka K,Yumoto-Yoshioka Y,Fleury F,Takahashi M

更新日期：2003-05-01 00:00:00