Abstract:
:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data derived from oscillation photographs indicate that the crystals belong to the monoclinic system and space group P2(1). The crystals have unit-cell dimensions of a = 45.4 A, b = 62.0 A, c = 99.7 A, and beta = 90.8 degrees. Assuming that the asymmetric unit contains two molecules, the Vm value was calculated to be 2.35 A(3).Da(-1). The crystals diffracted X-rays to at least 2.1 A resolution and were suitable for high-resolution X-ray crystal structure determination.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu Sdoi
10.1093/oxfordjournals.jbchem.a022588keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
9-11issue
1eissn
0021-924Xissn
1756-2651journal_volume
127pub_type
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123635
更新日期:1991-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003106
更新日期:2002-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133951
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更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-06-01 00:00:00
abstract::Capacitative Ca(2+) entry is essential for refilling intracellular Ca(2+) stores and is thought to be regulated primarily by inositol 1, 4,5-trisphosphate (IP(3))-sensitive stores in nonexcitable cells. In nonexcitable A549 cells, the application of caffeine or ryanodine induces Ca(2+) release in the absence of extrac...
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pub_type: 杂志文章
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更新日期:1985-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00