Abstract:
:Chicken liver mitochondria contained two forms of phosphoenolpyruvate (PEP) carboxykinase (designated as Mt-I and Mt-II) and the chicken liver cytosol fraction contained three forms of PEP carboxykinase (designated as Sol-I, Sol-II, and Sol-III). Mt-I and Mt-II were purified to homogeneity. They both had a molecular weight of 72,000 as judged by the sodium dodecyl-sulfate-polyacrylamide gel electrophoresis method, whereas an apparent molecular weight of 42,000 was obtained for both enzymes when estimated by Sephadex G-100 gel filtration. Studies with a rabbit antibody against the purified Mt-I revealed that Mt-I, Mt-II, Sol-I, and Sol-II are immunochemically identical, whereas Sol-III is immunochemically different from any of the other proteins. Genetic analogy between Mt-I and Mt-II was also suggested by gel electrophoretic analysis of their peptide maps. The content of Sol-III in the adult chicken liver was very small, whereas the livers of chick embryo and very young chick contained a considerable amount of Sol-III. The level of Sol-III in the adult chicken, however, could be significantly increased by the administration of hydrocortisone or isoproterenol. Apparently Sol-III is a cytosol-specific PEP carboxykinase which is similar to the cytosolic PEP carboxykinases of various mammals. Sol-III showed a molecular weight of about 60,000 as estimated by gel filtration. Studies with combined use of the antibody and [3H]leucine revealed that some portion of PEP carboxykinase (Sol-I plus Sol-II) appearing in the liver cytosol corresponds to a precursor in transit to the mitochondria, although major portions of Sol-I and Sol-II appear to be accounted for by release of the mitochondrial enzymes.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Kochi H,Serizawa K,Kikuchi Gdoi
10.1093/oxfordjournals.jbchem.a133045subject
Has Abstractpub_date
1980-09-01 00:00:00pages
895-904issue
3eissn
0021-924Xissn
1756-2651journal_volume
88pub_type
杂志文章abstract::We obtained enhanced thermostability by replacing Ser161 with Cys in subtilisin E from Bacillus subtilis, a cysteine-free alkaline serine protease. The Ser161Cys mutant subtilisin E was purified from the culture supernatant of the recombinant B. subtilis in an oxidizing environment. SDS-polyacrylamide gel electrophore...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022790
更新日期:2000-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132549
更新日期:1979-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134681
更新日期:1984-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvh017
更新日期:2004-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124357
更新日期:1994-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130737
更新日期:1975-02-01 00:00:00
abstract::Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132254
更新日期:1978-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022474
更新日期:1999-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122596
更新日期:1988-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134409
更新日期:1983-09-01 00:00:00
abstract::Enterococcus hirae V-ATPase, in contrast to most V-type ATPases, is resistant to N-ethylmaleimide (NEM). Alignment of the amino acid sequences of NtpA suggests that the NEM-sensitive Cys of V-type ATPases is replaced by Ala in E. hirae V-ATPase. Consistent with this prediction, the V-ATPase became sensitive upon subst...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003288
更新日期:2002-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135050
更新日期:1985-01-01 00:00:00
abstract::Proteins in the brush border membrane (BBM) of the midgut binding to the insecticidal Cry1Ac toxin from Bacillus thuringiensis were investigated to examine the lower sensitivity of Bombyx mori to Cry1Ac, and new aminopeptidase N that bound to Cry1Ac was discovered. DEAE chromatography of Triton X-100-soluble BBM prote...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj024
更新日期:2006-02-01 00:00:00
abstract::Palmitoyl CoA inhibited EDTA-ATPase of heavy meromyosin (HMM) prepared from rabbit skeletal muscle. The concentration for half maximum inhibition of EDTA-ATPase was about 18 microM. Myristoyl CoA, the other long chain fatty acyl CoA, also inhibited EDTA-HMM ATPase, but CoA and short chain CoA thioesters, such as butyr...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133530
更新日期:1981-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124700
更新日期:1995-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122185
更新日期:1987-12-01 00:00:00
abstract::Thrombomodulin (TM) is a thrombin receptor on the surface of endothelial cells that converts thrombin from a procoagulant to an anticoagulant. Thrombin promotes invasion by various tumor cells, and positive or negative correlations are found between the expression of TM and tumorigenesis in some patients. In this stud...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130799
更新日期:1975-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124987
更新日期:1995-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123774
更新日期:1992-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123567
更新日期:1991-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003247
更新日期:2002-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134069
更新日期:1982-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122074
更新日期:1987-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123949
更新日期:1992-11-01 00:00:00