Abstract:
:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000). Gp-2 was one of the major fractions in the Pronase digest of a sea-squirt antigen, Gi-rep, and is capable of eliciting the skin reaction specific to patients with sea-squirt allergy similarly to Gi-rep. An additional disaccharide structure of GalNAc1----(3/4)HexNAc1---- was also expected as the other major non-reducing terminal unit, though the anomeric configuration of the terminal GalNAc could not be identified. As Gp-2 carbohydrate was found to contain 3 mol each of the two types of terminal GalNAc-containing units, both were nominated as possible components of the IO4- oxidation-sensitive epitopes which are responsible for the allergenic activity in Gp-2 and its mother antigen, Gi-rep. A few moles of Fuc and Gal were also detected as minor non-reducing terminals in addition to the 6 mol of the GalNAc-containing units.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka Sdoi
10.1093/oxfordjournals.jbchem.a122728subject
Has Abstractpub_date
1989-05-01 00:00:00pages
691-6issue
5eissn
0021-924Xissn
1756-2651journal_volume
105pub_type
杂志文章abstract::A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135005
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133794
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132355
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更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1976-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124392
更新日期:1994-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123958
更新日期:1992-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122895
更新日期:1989-10-01 00:00:00
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更新日期:1991-07-01 00:00:00
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更新日期:2000-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvi105
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133093
更新日期:1980-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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更新日期:1993-12-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1977-05-01 00:00:00
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更新日期:1991-05-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1982-04-01 00:00:00
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更新日期:2010-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131660
更新日期:1977-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00