Presence of 1-O-alk-1'-enyl-2-O-acetyl glycerophosphocholine (vinyl form of PAF) in perfused rat and guinea pig hearts.

abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...

journal_title：Journal of biochemistry

authors： Katayama E,Wakabayashi T,Reinach F,Masaki T,Fischman DA

更新日期：1984-03-01 00:00:00

abstract：:The stimulatory effects of glucose and lactate on protein synthesis by round spermatids (steps 1-8) were further studied. When the cells were incubated with lactate, the response of protein synthesis in round spermatids was closely related to the intracellular level of ATP. The ATP level in spermatids increased to 3.1...

journal_title：Journal of biochemistry

authors： Nakamura M,Hino A,Kato J

更新日期：1981-10-01 00:00:00

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:Methylation and demethylation of the methyl-accepting chemotaxis protein (MCP) was studied in vitro. The in vitro MCP methylating system showed the following characteristics. 1. Multiple bands of methylated MCP were produced. 2. The reaction could be separated into two phases: the initial phase, in which the level of ...

journal_title：Journal of biochemistry

authors： Hayashi H,Minoshima S,Ohba M

更新日期：1982-08-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...

journal_title：Journal of biochemistry

authors： Ohe T,Watanabe Y

更新日期：1979-07-01 00:00:00

abstract：:High mobility group box 1 (HMGB1) protein is the most abundant chromatin-associated non-histone protein expressed in all nucleated eukaryotic cells. We examined the phosphorylation of mammalian HMGB1 by testing the ability of the cyclin-dependent kinase 5 (Cdk5) to use as substrates native protein, either unmodified o...

journal_title：Journal of biochemistry

authors： Ugrinova I,Pashev IG,Pasheva EA

更新日期：2011-05-01 00:00:00

abstract：:The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide fragment generated by Achromobacter protease I (API) digestion was purified and characterized by amino acid analysis and amino...

journal_title：Journal of biochemistry

authors： Minamitake Y,Kodama S,Katayama T,Adachi H,Tanaka S,Tsujimoto M

更新日期：1990-02-01 00:00:00

abstract：:The virion-associated RNA-dependent RNA polymerase of influenza virus PR8 was activated by treatment with specific non-ionic detergents, and was remarkably stimulated by exogenously added oligonucleotide primers. Among synthetic oligonucleotides of various chain lengths, the dinucleotide ApG and the trinucleotide ApGp...

journal_title：Journal of biochemistry

authors： Kawakami K,Ishihama A,Ohtsuka E,Tanaka T,Takashima H,Ikehara M

更新日期：1981-06-01 00:00:00

abstract：:We previously showed that fatty liver was easily induced in suncus by starvation and that the plasma level of apolipoprotein B (apo B) was very low. There are three possible explanations for the low level of apo B in the animals: low synthetic rate, low secretion rate, and rapid catabolism in the circulation of apo B....

journal_title：Journal of biochemistry

authors： Nagayoshi A,Matsuki N,Saito H,Tsukamoto K,Kaneko K,Wakashima M,Kinoshita M,Yamanaka M,Teramoto T

更新日期：1995-04-01 00:00:00

abstract：:Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

journal_title：Journal of biochemistry

authors： Shishido H,Yamada MD,Kondo K,Maruta S

更新日期：2009-10-01 00:00:00

abstract：:The asparagine-linked sugar chains of human follicle-stimulating hormone (hFSH) were liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. Ninety-five percent of the oligosaccharides were acidic and all were converted to a mixture of neu...

journal_title：Journal of biochemistry

authors： Renwick AG,Mizuochi T,Kochibe N,Kobata A

更新日期：1987-05-01 00:00:00

abstract：:Dopamine D2 receptor binding subunits of the porcine anterior pituitary were visualized by autoradiography following photoaffinity labeling with [125I]N-azidophenethylspiperone and sodium dodecyl-sulfate polyacrylamide gel electrophoresis. The ligand binding subunit comprising the pituitary D2 dopamine receptor migrat...

journal_title：Journal of biochemistry

authors： Jarvie KR,Niznik HB

更新日期：1989-07-01 00:00:00

abstract：:p-Nitrophenyl O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha- D-glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-alpha-D-glucopyranoside (FG5P) is hydrolyzed by human pancreatic a-amylase (HPA) or salivary alpha-amylase (HSA) to O-6-deoxy-6-[(2-pyridy...

journal_title：Journal of biochemistry

authors： Omichi K,Ikenaka T

更新日期：1986-11-01 00:00:00

abstract：:A method for immobilizing oligosaccharides on a TLC plate for immunostaining has been developed. N-Glycolylneuraminic acid (NeuGc)-containing oligosaccharides derived from II3NeuGc-LacCer, IV3NeuGc-nLcOse4Cer, II3NeuGc-GgOse3Cer, and II3(NeuGc)2-LacCer by digestion with our newly isolated endoglycoceramidase (Ito, M. ...

journal_title：Journal of biochemistry

authors： Higashi H,Hirabayashi Y,Ito M,Yamagata T,Matsumoto M,Ueda S,Kato S

更新日期：1987-08-01 00:00:00

abstract：:An artificial gene coding for the human cap binding protein (hCBP: human IF-4E) was chemically synthesized and expressed in Escherichia coli under the control of a trp promoter. The DNA duplex of 662 bp was designed and constructed from 44 oligodeoxynucleotide fragments of typically 30 nucleotides in length. Although ...

journal_title：Journal of biochemistry

authors： Ueda H,Maruyama H,Doi M,Inoue M,Ishida T,Morioka H,Tanaka T,Nishikawa S,Uesugi S

更新日期：1991-06-01 00:00:00

abstract：:Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracel...

journal_title：Journal of biochemistry

authors： Kimura T,Yoshida A,Tabuchi Y,Ikari A,Takeguchi N,Asano S

更新日期：2002-06-01 00:00:00

abstract：:The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Norioka S,Teshima T,Ikenaka T

更新日期：1987-03-01 00:00:00

abstract：:Glycerol-treated muscle fiber bundles were fixed at their rest length in 50 mM KC1, 2 mM MgC1(2), and 10 micron CaC1(2) at pH 7.8 and 0 degrees C in the presence of sufficient amounts of ATP, creatine kinase, and creatine phosphate. The fiber bundles were stretched linearly with time for 0.3 s at a constant amplitude,...

journal_title：Journal of biochemistry

authors： Arata T,Mukohata Y,Tonomura Y

更新日期：1978-10-01 00:00:00

abstract：:Oligosaccharides of a non-oligomannoside type were released from porcine alpha-mannosidase by hydrazinolysis, and were fractionated into at least 15 homogeneous oligosaccharides. Most of them are oligosaccharides with galactose and N-acetylglucosamine residues attached to a common core, alpha Man2 beta Man beta GlcNAc...

journal_title：Journal of biochemistry

authors： Kozutsumi Y,Kawasaki T,Yamashina I

更新日期：1986-08-01 00:00:00

abstract：:Heat-stable malate dehydrogenase isolated from Thermus flavus AT62 was completely inactivated by treatment with diethylpyrocarbonate. The inactivation was accompanied by the loss of 1.2 histidine residues per subunit of the enzyme. The enzyme was protected from inactivation by NADH. The enzyme was also inactivated by ...

journal_title：Journal of biochemistry

authors： Iijima S,Oh MJ,Saiki T,Beppu T

更新日期：1986-06-01 00:00:00

abstract：:We previously reported that novel Mg(2+)-ATPases were induced in rat liver peroxisomes by clofibrate administration and that these activities consisted of at least two types of enzymes, N-ethylmaleimide (NEM)-sensitive and -resistant. Here we present evidence that neither of these major peroxisomal ATPases is associat...

journal_title：Journal of biochemistry

authors： Shimizu S,Imanaka T,Takano T,Ohkuma S

更新日期：1992-12-01 00:00:00

abstract：:The circadian change of gluconeogenesis in the liver in vivo in fed rats was studied. Gluconeogenesis in the liver in vivo was determined by measuring the rate of synthesis of 14C-labeled blood glucose derived from a 14C-labeled substrate, such as lactate, pyruvate or an amino acid injected into nephrectomized rats. G...

journal_title：Journal of biochemistry

authors： Kida K,Nishio T,Yokozawa T,Nagai K,Matsuda H,Nakagawa H

更新日期：1980-10-01 00:00:00

abstract：:We designed mutant lysozymes with N-glycosylation signal sequences (Asn48-Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkholder minimum medium, N-glycosylation occurred in both lysozymes....

journal_title：Journal of biochemistry

authors： Ueda T,Iwashita H,Hashimoto Y,Imoto T

更新日期：1996-01-01 00:00:00

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00

abstract：:The isotypes of sarcoplasmic Ca2+ binding protein (SCP) were purified from shrimp tail muscle. SCP exists in a dimeric form. One sample of shrimp contained only alpha A chain, whereas another contained alpha B and beta chains, and a heterodimer of alpha B beta which was not analyzed precisely. The amino acid sequences...

journal_title：Journal of biochemistry

authors： Takagi T,Konishi K

更新日期：1984-06-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...

journal_title：Journal of biochemistry

authors： Okazaki N,Hazeki K,Izumi T,Nigorikawa K,Hazeki O

更新日期：2011-03-01 00:00:00