Affinity chromatography of cysteine-containing histone.

abstract：:Coenzyme A (CoASH) is an obligate cofactor for lipids undergoing beta-oxidation in peroxisomes. Although the peroxisomal membrane appears to be impermeable to CoASH, peroxisomes contain their own pool of CoASH. It is believed that CoASH enters peroxisomes as acyl-CoAs, but it is not known how this pool is regulated. T...

journal_title：Journal of biochemistry

authors： Reilly SJ,Tillander V,Ofman R,Alexson SE,Hunt MC

更新日期：2008-11-01 00:00:00

abstract：:HSP47 is a stress protein (heat shock protein) which resides in the endoplasmic reticulum, and is postulated to function as a collagen-specific molecular chaperone. To elucidate the role of HSP47 in procollagen biosynthesis, we have established human embryonic kidney 293 cell lines, which were stably transfected with ...

journal_title：Journal of biochemistry

authors： Hosokawa N,Hohenadl C,Satoh M,Kühn K,Nagata K

更新日期：1998-09-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:F-Actin was digested with alpha-chymotrypsin in 6 M urea, and two peptide fragments from subdomain 4 of actin molecule [Kabsch, W., Mannherz, H.G., Suck, D., Pai, E.F., & Holmes K.C. (1990) Nature 347, 37-44] were purified by reverse-phase HPLC and Sephadex G-50 gel filtration. The peptide fragments were identified as...

journal_title：Journal of biochemistry

authors： Hori K,Morita F

更新日期：1992-09-01 00:00:00

abstract：:Of the five HSV1 immediate-early proteins, infected-cell protein 22 (ICP22), the product of the Us1 gene, is a member whose function is less understood. In order to promote better understanding of the role of ICP22 in viral replication, mutation and fluorescence techniques were used to investigate the biochemical rela...

journal_title：Journal of biochemistry

authors： Cun W,Hong M,Liu LD,Dong CH,Luo J,Li QH

更新日期：2006-07-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00

abstract：:Gp91(phox) is a key component of the phagocyte NADPH oxidase. Mutations of its promoter found in patients with chronic granulomatous disease cause deficient binding of PU.1 and HAF-1. Because the two factors bind to the same site (Pu box) of the promoter, we attempted to clarify their relative in vivo contributions to...

journal_title：Journal of biochemistry

authors： Islam MR,Fan C,Fujii Y,Hao LJ,Suzuki S,Kumatori A,Yang D,Rusvai E,Suzuki N,Kikuchi H,Nakamura M

更新日期：2002-04-01 00:00:00

abstract：:Mutations of epsilon-sarcoglycan gene (SGCE) have been implicated in myoclonus-dystonia (M-D), a movement disorder. To determine the pathophysiology of M-D, we produced Sgce knockout mice and found that the knockout mice exhibited myoclonus, motor impairments, hyperactivity, anxiety, depression, significantly higher l...

journal_title：Journal of biochemistry

authors： Yokoi F,Dang MT,Li J,Li Y

更新日期：2006-07-01 00:00:00

abstract：:The complete amino acid sequence of acylphosphatase from rabbit skeletal muscle has been elucidated by automatic Edman degradation of peptides obtained from staphylococcal protease and trypsin digestions. The enzyme consisted of a single polypeptide chain of 98 amino acid residues, lacking only histidine. Its amino (N...

journal_title：Journal of biochemistry

authors： Kizaki T,Takasawa T,Mizuno Y,Shiokawa H

更新日期：1985-04-01 00:00:00

abstract：:It has been reported that two kinds of Cd-binding peptide (Cd-BP1 and Cd-BP2) are induced in fission yeast upon exposure to Cd, and that they consist of the same unit peptide (cadystin), but Cd-BP1 binds 1.5 times more Cd atoms per cadystin than Cd-BP2 (Murasugi, A., Wada, C., & Hayashi, Y. (1981) J. Biochem. 90, 1561...

journal_title：Journal of biochemistry

authors： Murasugi A,Wada Nakagawa C,Hayashi Y

更新日期：1984-11-01 00:00:00

abstract：:Glycerol dehydrogenase [EC 1.1.1.6] (pI 5.9) from Geotrichum candidium is effectively adsorbed in the presence of 20 mM acute buffer (pH 6.0) on either octyl-Sepharose or 10-carboxydecyl-Sepharose, among ten different kinds of n-alkyl-Sepharose derivatives tested, some of which have an amino or a carboxyl group as a t...

journal_title：Journal of biochemistry

authors： Sasaki I,Itoh N,Goto H,Yamamoto R,Tanaka H,Yamashita K,Yamashita J,Horio T

更新日期：1982-01-01 00:00:00

abstract：:Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

journal_title：Journal of biochemistry

authors： Sato S,Imamura S,Ozeki Y,Kawaguchi A

更新日期：1992-01-01 00:00:00

abstract：:Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007...

journal_title：Journal of biochemistry

authors： Tang F,Lord MS,Stallcup WB,Whitelock JM

更新日期：2018-05-01 00:00:00

abstract：:The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

journal_title：Journal of biochemistry

authors： Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:The TATA box-binding factor TFIID mediates transcriptional regulation through interactions with various regulatory factors and putative participation in reconfiguration of nucleosomes, utilizing its components, which include TATA box-binding protein (TBP) and TBP-associated factors (TAFs). Our and other previous studi...

journal_title：Journal of biochemistry

authors： Bando M,Ijuin S,Hasegawa S,Horikoshi M

更新日期：1997-03-01 00:00:00

abstract：:Interactions between phospholipid membranes and the acyl chain and specific amino acid residues of myristoylated proteins are necessary for membrane association. In the present study we tested the effects of mutations of calcineurin B subunit amino acid residues K(20)K(21), K(24)R(25), K(27)K(28) to Glu on the interac...

journal_title：Journal of biochemistry

authors： Martin BA,Oxhorn BC,Rossow CR,Perrino BA

更新日期：2001-05-01 00:00:00

abstract：:The gizzard tropomyosin molecule is composed of two subunits at 1:1 molar ratio. Possible composites of the tropomyosin molecule are two kinds of homodimer (one for each subunit), a heterodimer of two subunits, or a mixture of heterodimer and homodimer(s). We tried to evaluate the native subunit composition of gizzard...

journal_title：Journal of biochemistry

authors： Nakamura S,Sakurai T,Nonomura Y

更新日期：1991-05-01 00:00:00

abstract：:The present study was undertaken in an attempt to clarify the pathway by which hyperosmotic stress induces HB-EGF gene expression in rat aortic smooth muscle cells (RASMC). Hyperosmotic stress induced by a high concentration of glucose or mannitol resulted in an increase in HB-EGF mRNA level in a dose- and time-depend...

journal_title：Journal of biochemistry

authors： Koh YH,Che W,Higashiyama S,Takahashi M,Miyamoto Y,Suzuki K,Taniguchi N

更新日期：2001-09-01 00:00:00

abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...

journal_title：Journal of biochemistry

authors： Isemura S,Saitoh E,Ito S,Isemura M,Sanada K

更新日期：1984-10-01 00:00:00

abstract：:Archaeal geranylgeranyl diphosphate (GGPP) synthase catalyzes the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce GGPP with significant amounts of intermediates. To obtain information about the amino acids involved in the condensation and the release of intermediates, we ...

journal_title：Journal of biochemistry

authors： Ohnuma S,Hemmi H,Ohto C,Nakane H,Nishino T

更新日期：1997-04-01 00:00:00

abstract：:Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influence its DNA-binding a...

journal_title：Journal of biochemistry

authors： Xue Y,Wang S,Feng X

更新日期：2009-07-01 00:00:00

abstract：:In order to stabilize human RNase 1 by introduction of an intramolecular cross-link, a mutant protein (4-118CL RNase 1), in which Arg4 and Val118 are replaced with cysteine residues and linked by a disulfide bond, was designed and expressed in Escherichia coli as inclusion bodies. The 4-118CL RNase 1 that refolded und...

journal_title：Journal of biochemistry

authors： Futami J,Tada H,Seno M,Ishikami S,Yamada H

更新日期：2000-08-01 00:00:00

abstract：:Enterococcus hirae V-ATPase, in contrast to most V-type ATPases, is resistant to N-ethylmaleimide (NEM). Alignment of the amino acid sequences of NtpA suggests that the NEM-sensitive Cys of V-type ATPases is replaced by Ala in E. hirae V-ATPase. Consistent with this prediction, the V-ATPase became sensitive upon subst...

journal_title：Journal of biochemistry

authors： Murata T,Yoshikawa Y,Hosaka T,Takase K,Kakinuma Y,Yamato I,Kikuchi T

更新日期：2002-11-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...

journal_title：Journal of biochemistry

authors： Fukuhara M,Nohmi T,Mizokami K,Sunouchi M,Ishidate M Jr,Takanaka A

更新日期：1989-08-01 00:00:00

abstract：:Oligosaccharides of a non-oligomannoside type were released from porcine alpha-mannosidase by hydrazinolysis, and were fractionated into at least 15 homogeneous oligosaccharides. Most of them are oligosaccharides with galactose and N-acetylglucosamine residues attached to a common core, alpha Man2 beta Man beta GlcNAc...

journal_title：Journal of biochemistry

authors： Kozutsumi Y,Kawasaki T,Yamashina I

更新日期：1986-08-01 00:00:00

abstract：:Fluorescence changes of 1-anilino-8-naphthalenesulfonic acid and 3,3'-dipropyl-2,2'-thiadicarbocyanine during Ca transport by sarcoplasmic reticulum vesicles were studied. The fluorescence of both probes is enhanced corresponding to the rapid initial Ca uptake. The enhancement could be interpreted in terms of increase...

journal_title：Journal of biochemistry

authors： Ueno T,Sekine T

更新日期：1978-10-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00