A cluster of basic amino acid residues in calcineurin b participates in the binding of calcineurin to phosphatidylserine vesicles.

abstract：:The degranulation reactions of human neutrophils induced by 1-oleoyl-2-acetylglycerol (OAG), phorbol 12-myristate 13-acetate (PMA), and calcium ionophore A23187 or their combinations, were studied. OAG in the absence of the Ca2+-ionophore A23187 stimulated the releases of both lysozyme and lactoferrin, constituents of...

journal_title：Journal of biochemistry

authors： Kang D,Tsuda H,Takeshige K,Shibata Y,Minakami S

更新日期：1985-12-01 00:00:00

abstract：:Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

journal_title：Journal of biochemistry

authors： Mikami B,Nomura K,Morita Y

更新日期：1983-07-01 00:00:00

abstract：:Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an inte...

journal_title：Journal of biochemistry

authors： Nagai A,Ohta D

更新日期：1994-01-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:Acetoxy Drug: Protein Transacetylase (TAase) mediating the transfer of acetyl group(s) from polyphenolic acetates (PA) to certain functional proteins in mammalian cells was identified by our earlier investigations. TAase activity was characterized in the cell lysates of Mycobacterium smegmatis and the purified protein...

journal_title：Journal of biochemistry

authors： Gupta G,Baghel AS,Bansal S,Tyagi TK,Kumari R,Saini NK,Ponnan P,Kumar A,Bose M,Saluja D,Patkar SA,Parmar VS,Raj HG

更新日期：2008-12-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:By employing EPR spectrometry with the aid of a spin-trapping agent, 5,5-dimethyl-1-pyrroline-1-oxide (DMPO), the generation of superoxide anion and hydroxyl radical was reevaluated during the respiratory burst of porcine and human neutrophils. Properly prepared resting neutrophils did not generate any spin-trapped ra...

journal_title：Journal of biochemistry

authors： Ueno I,Kohno M,Mitsuta K,Mizuta Y,Kanegasaki S

更新日期：1989-06-01 00:00:00

abstract：:Neuronal migration is a crucial event in neuronal development for the construction of brain architecture and neuronal networks. Newborn neurons proliferate in the germinal zone and start migration toward their final destination. Migrating neurons adopt different routes, cell shapes and migratory modes depending on ext...

journal_title：Journal of biochemistry

authors： Horigane SI,Ozawa Y,Yamada H,Takemoto-Kimura S

更新日期：2019-05-01 00:00:00

abstract：:The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

journal_title：Journal of biochemistry

authors： Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

更新日期：2000-07-01 00:00:00

abstract：:A membrane protein insoluble in water was isolated by gel chromatography in the presence of 0.1% sodium dodecyl sulfate (SDS) from chromatophores of a photosynthetic bacterium, Rhodospirillum rubrum. This is one of the major membrane proteins of the chromatophore. The protein was found to bind about four grams of SDS ...

journal_title：Journal of biochemistry

authors： Miyake J,Ochiai-Yanagi S,Kasumi T,Takagi T

更新日期：1978-06-01 00:00:00

abstract：:We generated and characterized novel antibodies specific for a cleavage site of human caspase-8/FLICE and its substrate, FLICE-like inhibitory protein (FLIP). The synthetic peptides used as immunogens were CQGDNYQKGIPVETD (#791) and VSEGQLEDSSLLEVD (#1342), which corresponded to cleaved regions of N-terminal fragments...

journal_title：Journal of biochemistry

authors： Niikura Y,Nonaka T,Imajoh-Ohmi S

更新日期：2002-07-01 00:00:00

abstract：:The enthalpies of transfer of several amino acids and diglycine from water to aqueous polyols (glycerol, xylitol, sorbitol, and inositol) have been calorimetrically determined in order to clarify the mechanism of polyol-induced stabilization of proteins. The obtained enthalpy data were combined with the data on free e...

journal_title：Journal of biochemistry

authors： Gekko K

更新日期：1981-12-01 00:00:00

abstract：:The nucleotide sequence at the intron-exon junction in the human beta-globin gene was analyzed by the quantification method (categorical discriminant analysis) proposed previously. Using the sample score of a 16-nucleotide sequence at a 3'-splice junction, we studied to what extent such a sequence contains the 3'-spli...

journal_title：Journal of biochemistry

authors： Iida Y

更新日期：1990-12-01 00:00:00

abstract：:The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

journal_title：Journal of biochemistry

authors： Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

更新日期：1993-05-01 00:00:00

abstract：:The effects of sphingoid bases, sphingosine and dihydrosphingosine, which are protein kinase C (PKC) inhibitors, on NADPH oxidase were examined in a cell-free system. The bases inhibited cell-free activation of NADPH oxidase by arachidonic acid at lower concentration than N-acetylsphingosine. Thus, positive charge in ...

journal_title：Journal of biochemistry

authors： Sasaki JI,Yamaguchi M,Saeki S,Yamane H,Okamura N,Ishibashi S

更新日期：1996-10-01 00:00:00

abstract：:A new p-coumaric acid (4-hydroxycinnamic acid) hydroxylase was detected in mung bean seedlings treated with tentoxin, a fungal toxin, in which polyphenol oxidase that hydroxylates a wide variety of monophenols in vitro was completely eliminated. The enzyme required molecular oxygen and showed a pH optimum of 5.0. The ...

journal_title：Journal of biochemistry

authors： Kojima M,Takeuchi W

更新日期：1989-02-01 00:00:00

abstract：:To study the effect of cross-linking of membrane proteins on vesiculation of human erythrocytes by dimyristoylphosphatidylcholine (DMPC), red cells were treated with diamide at atmospheric pressure or 100 MPa and then incubated with DMPC in buffers of pH 6.5-8.5. Irrespective of buffer pH, the amount of released vesic...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Yamada S,Kimoto E

更新日期：1994-04-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:The presence of NADH-cytochrome b5 reductase [EC 1.6.2.2] in microsomes from anaerobically grown yeast was confirmed by its isolation and purification. The purified preparation of the reductase showed an apparent molecular weight of 27,000 daltons. The reductase appeared to contain loosely-bound FAD as a prosthetic gr...

journal_title：Journal of biochemistry

authors： Kubota S,Yoshida Y,Kumaoka H

更新日期：1977-01-01 00:00:00

abstract：:The conditions for the preparation of the ferricytochrome a-ferrocytochrome a3-carbon monoxide complex (a3+, a3(2)+CO) of cytochrome oxidase [EC 1.9.3.1] by the ferricyanide-reoxidation method and some properties of the prepared complex were studied. The addition of a small volume of concentrated ferricyanide solution...

journal_title：Journal of biochemistry

authors： Horie S,Watanabe T,Ave K

更新日期：1983-04-01 00:00:00

abstract：:Hyperthermophilic bacteria Thermotoga maritima and Thermotoga hypogea produce ethanol as a metabolic end product, which is resulted from acetaldehyde reduction catalysed by an alcohol dehydrogenase (ADH). However, the enzyme that is involved in the production of acetaldehyde from pyruvate is not well characterized. An...

journal_title：Journal of biochemistry

authors： Eram MS,Wong A,Oduaran E,Ma K

更新日期：2015-12-01 00:00:00

abstract：:In the rat, DNA carrying a cluster of the genes for tRNAAsp, tRNAGly, and tRNAGlu, aligned in that order, is repeated about 10 times. Seven DNA clones corresponding to the independent repeating units were isolated from a rat gene library. Nucleotide sequence analysis of these clones revealed the presence of a fourth t...

journal_title：Journal of biochemistry

authors： Shibuya K,Noguchi S,Yamaki M,Nishimura S,Sekiya T

更新日期：1985-06-01 00:00:00

abstract：:Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodec...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-10-01 00:00:00

abstract：:Alginate, which is an anionic polysaccharide, is widely distributed in the cell wall of brown algae. Alginate and the products of its degradation (oligosaccharides) are used in stabilizers, thickeners and gelling agents, especially in the food industry. The degradation of alginate generally involves a combination of s...

journal_title：Journal of biochemistry

authors： Yagi H,Fujise A,Itabashi N,Ohshiro T

更新日期：2018-04-01 00:00:00

abstract：:Autotaxin (ATX) is an ecto-enzyme responsible for lysophosphatidic acid (LPA) production in blood. ATX is present in various biological fluids such as cerebrospinal and seminal fluids and accounts for bulk LPA production in these fluids. ATX is a member of the nucleotide pyrophosphatase/phosphodiesterase (NPP) family ...

journal_title：Journal of biochemistry

authors： Nakanaga K,Hama K,Aoki J

更新日期：2010-07-01 00:00:00

abstract：:The alkalophile NADH dehydrogenase (NADH: 2,6-dichlorophenolindophenol oxidoreductase) [EC 1.6.99.3] consists of two identical subunits of 65 kDa, and each subunit contains the catalytic and liposome-binding regions. On treatment with trypsin, the polypeptide exhibiting the liposome-binding property in one of the subu...

journal_title：Journal of biochemistry

authors： Xu XM,Kanaya S,Koyama N,Sekiguchi T,Nosoh Y,Ohashi S,Tsuda K

更新日期：1989-04-01 00:00:00

abstract：:Apoprotein of electron-transferring flavoprotein (ETF) reacts with FAD as follows: A*<-->A, A+FAD<-->holoETF. Two different forms of apoETF (A* and A) convert into each other and only one of them, A, can associate with FAD [Sato, K. et al. (1991) J. Biochem. 109, 734-740]. In the present study, the reactions between a...

journal_title：Journal of biochemistry

authors： Sato K,Nishina Y,Shiga K

更新日期：1992-12-01 00:00:00

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:Several trypsin inhibitors with different mobilities on polyacrylamide gel electrophoresis occur in the tubers of taro (Colocasia antiquorum), and they each have a dimeric molecular weight of 40,000. Of all the constituent subunits, molecular weight 20,000, of the taro trypsin inhibitor (TTI), three major subunit comp...

journal_title：Journal of biochemistry

authors： Ogata F,Makisumi S

更新日期：1985-02-01 00:00:00

abstract：:Protein phosphorylation is a key regulatory mechanism of the organization and dynamics of the actin cytoskeleton during cell motility, differentiation, and cytokinesis. The level of protein phosphorylation is dependent on the relative activities of both protein kinases and protein phosphatases. In this paper, we exami...

journal_title：Journal of biochemistry

authors： Usui T,Marriott G,Inagaki M,Swarup G,Osada H

更新日期：1999-05-01 00:00:00