Purification and properties of growth inhibitor from normal rabbit serum.

abstract：:The Ca2+-sensitive ATPase activity of rabbit skeletal myofibrils disappeared completely after treatment with a solution containing CDTA, a strong divalent cation chelator, at a low ionic strength. A gel electrophoretic study revealed that all troponin C and about half of myosin light chain 2 were removed from the myof...

journal_title：Journal of biochemistry

authors： Morimoto S,Ohtsuki I

更新日期：1987-02-01 00:00:00

abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

journal_title：Journal of biochemistry

authors： Maeng JH,Yoon JB

更新日期：1998-07-01 00:00:00

abstract：:The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...

journal_title：Journal of biochemistry

authors： Mori K

更新日期：2009-12-01 00:00:00

abstract：:While many reports have shown that Ca2+ alone causes ryanodine binding to the heavy fraction of the sarcoplasmic reticulum (HFSR), our results demonstrate that caffeine or beta,gamma-methylene adenosine triphosphate (AMPOPCP) in addition to Ca2+ is necessary for ryanodine binding, although Ca2+ is indispensable for it...

journal_title：Journal of biochemistry

authors： Ogawa Y,Harafuji H

更新日期：1990-06-01 00:00:00

abstract：:Paranitrophenylphosphate (pNPP) induced fluorescence changes in fluorescence isothiocyanate (FITC)-labeled Na+,K(+)-ATPase preparations. The extents of changes were similar to those induced by acetylphosphate (AcP) accompanying accumulation of a K(+)-sensitive phosphoenzyme (E2P) and an ouabain bound phosphoenzyme in ...

journal_title：Journal of biochemistry

authors： Yamazaki A,Kaya S,Tsuda T,Araki Y,Hayashi Y,Taniguchi K

更新日期：1994-12-01 00:00:00

abstract：:Aspartic proteases of human malarial parasites are thought to play key roles in essential pathways of merozoite release, invasion and host cell hemoglobin degradation during the intraerythrocytic stages of their life cycle. Therefore, we have purified and characterized Plasmodium vivax aspartic protease, to determine ...

journal_title：Journal of biochemistry

authors： Sharma A,Eapen A,Subbarao SK

更新日期：2005-07-01 00:00:00

abstract：:The denaturation of recombinant horse L-chain apoferritin (rLF), which is composed of 24 L-chain subunits, in acidic solution was studied. Using two rLF mutants, lacking four (Fer4) or eight (Fer8) N-terminal amino acid residues, the effect of N-terminal residues on the protein's stability was investigated. Of the two...

journal_title：Journal of biochemistry

authors： Yoshizawa K,Mishima Y,Park SY,Heddle JG,Tame JR,Iwahori K,Kobayashi M,Yamashita I

更新日期：2007-12-01 00:00:00

abstract：:We have purified L-kynurenine 3-monooxygenase from pig liver mitochondria using a procedure involving seven steps composed of (1) preparation of mitochondrial outer membrane, (2) preparation of the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) insoluble outer membrane mater...

journal_title：Journal of biochemistry

authors： Uemura T,Hirai K

更新日期：1998-02-01 00:00:00

abstract：:Rat platelets released phospholipase A2 and lysophospholipase upon activation with thrombin or ADP. The release of phospholipases was energy-dependent and was not in parallel with that of a known lysosomal marker enzyme, N-acetyl-beta-D-glucosaminidase. The phospholipases are derived from other granules (dense granule...

journal_title：Journal of biochemistry

authors： Horigome K,Hayakawa M,Inoue K,Nojima S

更新日期：1987-01-01 00:00:00

abstract：:A simple and rapid purification method for human serum C-reactive protein (CRP) was developed. CRP was strongly adsorbed on a DEAE-cellulose column and was easily separated from other serum proteins. CRP was purified approximately 1,000-fold with a high yield (50%). The final preparation showed a single band as judged...

journal_title：Journal of biochemistry

authors： Tsujimoto M,Inoue K,Nojima S

更新日期：1983-11-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:Myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. The Ca2+-, EDTA-, and Mg2+-ATPase [EC 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 M KCl at pH 7.0 and 25 degrees. The Ca2+-AT...

journal_title：Journal of biochemistry

authors： Takeuchi K,Shibata N,Senda N

更新日期：1975-07-01 00:00:00

abstract：:beta-Hydroxyisovalerylshikonin (beta-HIVS), which was isolated from the plant, Lithospermium radix, inhibited the growth of various lines of cancer cells derived from human solid tumors at low concentrations between 10(-8) and 10(-6) M. When HL-60 cells were treated with 10(-6) M beta-HIVS for 3 h, characteristic feat...

journal_title：Journal of biochemistry

authors： Hashimoto S,Xu M,Masuda Y,Aiuchi T,Nakajo S,Cao J,Miyakoshi M,Ida Y,Nakaya K

更新日期：1999-01-01 00:00:00

abstract：:In the rat liver, two mRNAs for serine:pyruvate (or alanine:glyoxylate) aminotransferase are generated from a single gene by alternative transcription initiation. The longer mRNA encodes a precursor of a mitochondrial enzyme that has a mitochondrial targeting signal at the N-terminus and is translocated into mitochond...

journal_title：Journal of biochemistry

authors： Oda T,Uchida C,Miura S

更新日期：2000-04-01 00:00:00

abstract：:Chondroitin sulphate proteoglycan 4 (CSPG4) is a cell surface proteoglycan highly expressed by tumour, perivascular and oligodendrocyte cells and known to be involved cell adhesion and migration. This study showed that CSPG4 was present as a proteoglycan on the cell surface of two melanoma cell lines, MM200 and Me1007...

journal_title：Journal of biochemistry

authors： Tang F,Lord MS,Stallcup WB,Whitelock JM

更新日期：2018-05-01 00:00:00

abstract：:In porcine aortic smooth muscle cells, neuropeptide Y (NPY) stimulates mobilization of CA(2+) from intracellular store sites via Y1 receptors. However, it has been debated whether or not Ca(2+) mobilization by Y1 receptors depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] following activation of...

journal_title：Journal of biochemistry

authors： Shigeri Y,Nakajima S,Fujimoto M

更新日期：1995-09-01 00:00:00

abstract：:Hen ovomucoid was chemically deglycosylated by treatment with trifluoromethanesulfonic acid at 0 degrees C for 60 min. About 75 mol% of the carbohydrate moiety was removed from the glycoprotein without changing its amino acid composition, and its trypsin inhibitory activity and immunoreactivity with specific antibodie...

journal_title：Journal of biochemistry

authors： Gu JX,Matsuda T,Nakamura R,Ishiguro H,Ohkubo I,Sasaki M,Takahashi N

更新日期：1989-07-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide fragment generated by Achromobacter protease I (API) digestion was purified and characterized by amino acid analysis and amino...

journal_title：Journal of biochemistry

authors： Minamitake Y,Kodama S,Katayama T,Adachi H,Tanaka S,Tsujimoto M

更新日期：1990-02-01 00:00:00

abstract：:Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide disc gel electrophoresis. The molecular weights of these enzymes w...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1977-03-01 00:00:00

abstract：:Pollen proteins from several grass species have been identified and characterized as causative allergens in grass pollinosis. In contrast, allergenic potential of pollen proteins from rice, which belongs to the same Poaceae family, has not well been investigated, despite that a few clinical cases have been reported on...

journal_title：Journal of biochemistry

authors： Hirano K,Hino S,Oshima K,Okajima T,Nadano D,Urisu A,Takaiwa F,Matsuda T

更新日期：2013-08-01 00:00:00

abstract：:Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC 4.1.2.13] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

journal_title：Journal of biochemistry

authors： Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

更新日期：1991-04-01 00:00:00

abstract：:A post-proline cleaving enzyme [post-proline endopeptidase: EC 3.4.21.26] was purified from lamb brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and Sephadex G-150. The purified enzyme appeared homogeneous on disc gel and sodium dodecyl sulfate (SDS) gel electrophoreses. The enzyme was mo...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Simmons WH,Kita T,Tsuru D

更新日期：1981-08-01 00:00:00

abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...

journal_title：Journal of biochemistry

authors： Yohda M,Yoshida M

更新日期：1987-10-01 00:00:00

abstract：:An open reading frame of 828 base pairs was found in the CHO1 gene region of Saccharomyces cerevisiae by nucleotide sequencing analysis. Its enhanced expression with the aid of the PHO5 regulatory sequence resulted in an overproduction of a protein with a molecular weight of approximately 30,000, which in turn was con...

journal_title：Journal of biochemistry

authors： Kiyono K,Miura K,Kushima Y,Hikiji T,Fukushima M,Shibuya I,Ohta A

更新日期：1987-11-01 00:00:00

abstract：:Rous sarcoma virus-transformed rat liver cell line RSV-BRL secreted a neutral proteinase in a latent precursor form with a molecular weight (Mr) of 57,000 (57k) as a major secreted protein. This enzyme was a calcium-dependent metallo-proteinase. The proenzyme was purified from the serum-free conditioned medium of the ...

journal_title：Journal of biochemistry

authors： Umenishi F,Yasumitsu H,Ashida Y,Yamauti J,Umeda M,Miyazaki K

更新日期：1990-10-01 00:00:00

abstract：:Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

journal_title：Journal of biochemistry

authors： Narutaki S,Dunn BM,Oda K

更新日期：1999-01-01 00:00:00

abstract：:Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an inte...

journal_title：Journal of biochemistry

authors： Nagai A,Ohta D

更新日期：1994-01-01 00:00:00

abstract：:Glycosphingolipids (GSLs), cell type-specific markers which change dramatically during ontogenesis and oncogenesis, have been implicated as playing major roles in cellular interactions and control of cell proliferation in multicellular organisms. These functional roles have been partially clarified through two types o...

journal_title：Journal of biochemistry

authors： Hakomori S,Igarashi Y

更新日期：1995-12-01 00:00:00

abstract：:Bacteriorhodopsin from Halobacterium halobium was stable and active in high concentrations of guanidine hydrochloride. However the decay of the intermediate with absorption maximum at 407 nm and the uptake of protons from the medium were remarkably delayed. At alkaline pH values, all the molecules of bacteriohodopsin ...

journal_title：Journal of biochemistry

authors： Yoshida M,Ohno K,Takeuchi Y

更新日期：1980-02-01 00:00:00