L-kynurenine 3-monooxygenase from mitochondrial outer membrane of pig liver: purification, some properties, and monoclonal antibodies directed to the enzyme.

abstract：:Previously, several workers reported that at very low ionic strength and in the presence of ATP, the extent of binding of S-1 with the F-actin-tropomyosin-troponin complex or regulated actin (FA-TM-TN) is unaffected by removal of Ca2+. However, in this study we found that during the ATPase reaction at physiological io...

journal_title：Journal of biochemistry

authors： Inoue A,Tonomura Y

更新日期：1982-04-01 00:00:00

abstract：:"DNase binding protein" of 43K daltons as determined by SDS-polyacrylamide gel electrophoresis, was purified from the 0.1 M KCl-soluble (non-structural) fraction of chicken skeletal muscle. The protein was distinct from actin in amino acid composition and physicochemical properties. "DNase binding protein" was also is...

journal_title：Journal of biochemistry

authors： Kohama K,Holtzer H

更新日期：1981-02-01 00:00:00

abstract：:We recently demonstrated, using synthetic peptides modeled on the extension peptide of malate dehydrogenase, that amino acid residues present at the proximal and distal positions relative to the cleavage site are critical determinants for the recognition of substrates by mitochondrial processing peptidase [Niidome et ...

journal_title：Journal of biochemistry

authors： Moriwaki K,Ogishima T,Ito A

更新日期：1999-11-01 00:00:00

abstract：:The 20,000 dalton light chain (L2) was isolated from rabbit and chicken striated muscle myosins, and the Ca2+-induced conformational changes of these proteins were investigated. 1) The reaction of thiol groups of L2 with dithiobisnitrobenzoic acid (DTNB), 2) measurements of the UV difference absorption spectrum, 3) me...

journal_title：Journal of biochemistry

authors： Minowa O,Matsuda S,Yagi K

更新日期：1983-07-01 00:00:00

abstract：:Type A atrial natriuretic peptide (ANP) receptor was demonstrated to be present as a tetramer in the bovine adrenal cortex. Type A ANP receptor is composed of two functional domains, namely extracellular ANP-binding and cytoplasmic guanylate cyclase domains, and generally considered to be present as a single polypepti...

journal_title：Journal of biochemistry

authors： Iwata T,Uchida-Mizuno K,Katafuchi T,Ito T,Hagiwara H,Hirose S

更新日期：1991-07-01 00:00:00

abstract：:The spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, had a complex spectrum of neutral glycosphingolipids. CDS, CTS, CTeS were isolated as minor components (together they accounted for 3% of the total neutral glycolipid fraction), while CMS and the glycolipids (Lipid I, II, and III) with larger saccharide...

journal_title：Journal of biochemistry

authors： Sugita M,Yamamoto T,Masuda S,Itasaka O,Hori T

更新日期：1981-11-01 00:00:00

abstract：:NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

journal_title：Journal of biochemistry

authors： Shioi Y,Takamiya K,Nishimura M

更新日期：1976-02-01 00:00:00

abstract：:Glutathione-S-epoxide transferase has been purified from guinea pig liver in a homogeneous form having a sedimentation coefficient, s20,w of 3.8S, and a molecular weight of 46,000, and dissociable into subunits with a molecular weight of 25,000. The activities with four substrates, styrene oxide, naphthalene oxide, io...

journal_title：Journal of biochemistry

authors： Hayakawa T,Myokei Y,Yagi H,Jerina DM

更新日期：1977-08-01 00:00:00

abstract：:A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

journal_title：Journal of biochemistry

authors： Miyazawa K,Mori A,Okudaira H

更新日期：1998-12-01 00:00:00

abstract：:The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

journal_title：Journal of biochemistry

authors： Nakajima Y,Nakamura K

更新日期：1991-02-01 00:00:00

abstract：:Amino acids produced from formaldehyde and hydroxylamine in modified sea mediums with different concentrations of molybdate were analyzed. The modified sea mediums contained lower concentration of sodium chloride and higher concentrations of transition metal ions (Zn2+, Fe3+, Cu2+, Co2+, Mn2+ each 10(-4)m, and Mo O4(2...

journal_title：Journal of biochemistry

authors： Hatanaka H,Egami F

更新日期：1977-08-01 00:00:00

abstract：:The reaction of D-amino acid oxidase [EC 1.4.3.3] (DAO) from porcine kidney with beta-cyano-D-alanine (D-BCNA) was studied. DAO was found to catalyze elimination of the cyano group as well as oxidation of D-BCNA. During the course of the reaction in the presence of excess oxygen, an intermediate was observed which exh...

journal_title：Journal of biochemistry

authors： Miura R,Shiga K,Miyake Y,Watari H,Yamano T

更新日期：1980-05-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00

abstract：:Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracel...

journal_title：Journal of biochemistry

authors： Kimura T,Yoshida A,Tabuchi Y,Ikari A,Takeguchi N,Asano S

更新日期：2002-06-01 00:00:00

abstract：:Protein S, a vitamin K-dependent plasma protein having Gla-residues, increases the rate of inactivation of Factor Va by activated protein C by enhancing the binding of activated protein C to phospholipid [Walker, J.F. (1981) J. Biol. Chem. 256, 11128-11131]. The present study aimed at elucidating the effect of thrombi...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hashimoto S

更新日期：1983-09-01 00:00:00

abstract：:An enzyme activity specifically active on nitrous acid-treated DNA was found in an extract of Escherichia coli. The enzyme acts on both double- and single-stranded DNAs, treated with nitrous acid, in the presence of EDTA, although the former DNA is a better substrate. Evidence is presented that nitrous acid- and bisul...

journal_title：Journal of biochemistry

authors： Oeda K,Shimizu K,Sekiguchi M

更新日期：1978-11-01 00:00:00

abstract：:The cDNA of a novel protein, which contains the association domain of alpha isoform of calmodulin-dependent protein kinase II (CaM-kinase II alpha), was cloned from rat skeletal muscle. This protein, called alpha KAP, consisted of 200 amino acid residues with a molecular weight of 22,583. alpha KAP has a highly hydrop...

journal_title：Journal of biochemistry

authors： Sugai R,Takeuchi M,Okuno S,Fujisawa H

更新日期：1996-10-01 00:00:00

abstract：:The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

journal_title：Journal of biochemistry

authors： Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

更新日期：1993-05-01 00:00:00

abstract：:alpha and beta chains from adult hemoglobin of the slender loris (Loris tardigradus) were isolated by Amberlite CG-50 column chromatography. After S-aminoethylation, both chains were digested with trypsin and the amino acid sequences of the tryptic peptides obtained were analyzed. Further, the order of these tryptic p...

journal_title：Journal of biochemistry

authors： Maita T,Goodman M,Matsuda G

更新日期：1978-08-01 00:00:00

abstract：:Thermal denaturation of cytochromes c of horse, cow, and Candida krusei in aqueous guanidine hydrochloride in the neutral pH region was studied by means of absorption and optical rotation measurements. The values of standard free energy change upon denaturation were estimated over the temperature range from 3 to 51 de...

journal_title：Journal of biochemistry

authors： Kawaguchi H,Noda H

更新日期：1977-05-01 00:00:00

abstract：:We examined the effects of growth factors on intracellular free Mg2+ concentrations ([Mg2+]i) in single Swiss 3T3 fibroblasts, using microfluorometry of a Mg(2+)-sensitive dye mag-fura-2. We had already noted an increase in [Mg2+]i after exposure to bombesin for 30-60 min [Ishijima, S., Sonoda, T., & Tatibana, M. (199...

journal_title：Journal of biochemistry

authors： Ishijima S,Tatibana M

更新日期：1994-04-01 00:00:00

abstract：:Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from lambda gt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without th...

journal_title：Journal of biochemistry

authors： Zhang R,Yatsuki H,Kusakabe T,Iwabe N,Miyata T,Imai T,Yoshida M,Hori K

更新日期：1995-03-01 00:00:00

abstract：:The experimental conditions for release of the regulatory light chain (RLC) of scallop myosin at 30 degrees C were studied. Substantially all RLC was released from myosin by incubation for 5 min in medium containing buffer and KCl. This release of RLC was inhibited strongly by Ca2+, while the effect of Mg2+ was about ...

journal_title：Journal of biochemistry

authors： Asakawa T,Azuma N

更新日期：1983-08-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:A continuous cell-free protein synthesis system [Spirin et al. (1988) Science 242, 1162-1164] was modified so as to be suitable for coupled transcription-translation, a process useful for obtaining products of cloned genes or cDNAs. A reaction chamber equipped with an ultrafiltration membrane was newly designed and an...

journal_title：Journal of biochemistry

authors： Kigawa T,Yokoyama S

更新日期：1991-08-01 00:00:00

abstract：:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hem...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Matsumoto M,Kimoto E

更新日期：1995-10-01 00:00:00

abstract：:1. As a part of studies on the mechanism by which catecholamines are released from the nerve terminals, the synaptic vesicle fraction was isolated from bovine caudatolenticular nuclei and thalamus by differential centrifugation essentially according to the method of Kadota and Kadota (17). 2. Further centrifugation on...

journal_title：Journal of biochemistry

authors： Tsudzuki T,Fujii T,Tanaka R

更新日期：1977-09-01 00:00:00

abstract：:Using a new cannulation method, which completely prevented interruption of circulation to brain tissue during surgical operations, we could prepare a functionally intact hemoglobin-free isolated perfused rat head in situ. The frequency distribution (8-25 Hz) and amplitude of a spontaneous electroencephalogram, EEG (50...

journal_title：Journal of biochemistry

authors： Inagaki M,Tamura M

更新日期：1993-06-01 00:00:00

abstract：:This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

journal_title：Journal of biochemistry

authors： Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

更新日期：2020-03-01 00:00:00

abstract：:A gene encoding an Na+/H+ antiporter was cloned from chromosomal DNA of the slightly halophilic marine bacterium Vibrio parahaemolyticus. The host was an Escherichia coli mutant that lacked both of the two major Na+/H+ antiporters, NhaA and NhaB. Untransformed mutant cells were unable to grow in the presence of 0.6 M ...

journal_title：Journal of biochemistry

authors： Kuroda T,Shimamoto T,Inaba K,Tsuda M,Tsuchiya T

更新日期：1994-11-01 00:00:00