Abstract:
:1. As a part of studies on the mechanism by which catecholamines are released from the nerve terminals, the synaptic vesicle fraction was isolated from bovine caudatolenticular nuclei and thalamus by differential centrifugation essentially according to the method of Kadota and Kadota (17). 2. Further centrifugation on a sucrose density gradient of the synaptic vesicle fraction by the method of Whittaker et al. (1) yielded white materials on the upper portion of 0.4 M sucrose, which consisted of vesicles averaging 600-800 A in diameter, and did not show Mg2+-dependent ATpase activity. On the other hand, the denser materials centering on 0.6 M sucrose, consisting of a mixture of microsomes and synaptic vesicles of 400-500 A diameter, showed an ATpase activity activated by either Mg2+ or Ca2+ but not inhibited by ouabain. 3. The white materials on 0.4 M sucrose were almost free of mitochondria, but they contained a large amount of non-heme iron, as reported elsewhere (2). Furthermore, the protein components analyzed on SDS-polyacrylamide gels were similar to those already reported for purified synaptic vesicles (3). 4. Based on these results, the white materials were assumed to be synaptic vesicles devoid of Mg2+-dependent ATPase activity.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsudzuki T,Fujii T,Tanaka Rdoi
10.1093/oxfordjournals.jbchem.a131747keywords:
subject
Has Abstractpub_date
1977-09-01 00:00:00pages
709-17issue
3eissn
0021-924Xissn
1756-2651journal_volume
82pub_type
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更新日期:1982-05-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1985-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133480
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132823
更新日期:1980-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvm046
更新日期:2007-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134609
更新日期:1984-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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更新日期:2007-03-01 00:00:00
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更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1987-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-09-01 00:00:00