Abstract:
:Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsin with ovomucoid hindered the binding of the specific antibody at some antigenic sites of ovomucoid by lowering the antibody-binding affinity of these sites. The anti-ovomucoid antiserum was absorbed with ovomucoid-trypsin complex, and non-absorbed antibody was collected by immunoaffinity chromatography of ovomucoid-coupled Sepharose 4B. The antibody blocked the trypsin-inhibitory activity of ovomucoid in a molar ratio (antibody/ovomucoid) of about 1.2:1. The findings suggested that at least one antigenic site is located near the reactive site of trypsin inhibition (Arg89 decreases Ala90) of ovomucoid.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Matsuda T,Watanabe K,Nakamura Rdoi
10.1093/oxfordjournals.jbchem.a134352subject
Has Abstractpub_date
1983-07-01 00:00:00pages
43-9issue
1eissn
0021-924Xissn
1756-2651journal_volume
94pub_type
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journal_title:Journal of biochemistry
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134910
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pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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更新日期:1995-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1981-03-01 00:00:00
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doi:10.1093/oxfordjournals.jbchem.a121806
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133639
更新日期:1981-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135160
更新日期:1985-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121975
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更新日期:1980-07-01 00:00:00
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更新日期:1976-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135283
更新日期:1985-08-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1983-09-01 00:00:00
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更新日期:1985-01-01 00:00:00
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更新日期:1986-03-01 00:00:00
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更新日期:1993-12-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2000-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-04-01 00:00:00