Differential expression of laminin-5/ladsin subunits in human tissues and cancer cell lines and their induction by tumor promoter and growth factors.

abstract：:Proteins in the brush border membrane (BBM) of the midgut binding to the insecticidal Cry1Ac toxin from Bacillus thuringiensis were investigated to examine the lower sensitivity of Bombyx mori to Cry1Ac, and new aminopeptidase N that bound to Cry1Ac was discovered. DEAE chromatography of Triton X-100-soluble BBM prote...

journal_title：Journal of biochemistry

authors： Shitomi Y,Hayakawa T,Hossain DM,Higuchi M,Miyamoto K,Nakanishi K,Sato R,Hori H

更新日期：2006-02-01 00:00:00

abstract：:The protein level of the GTS1 gene product (Gts1p) fluctuated during the oscillation of energy metabolism in continuous culture of the yeast Saccharomyces cerevisiae. Here, we found that the GTS1 mRNA level oscillated with the same periodicity as the metabolic oscillation, suggesting that the expression of GTS1 was re...

journal_title：Journal of biochemistry

authors： Tonozuka H,Wang J,Mitsui K,Saito T,Hamada Y,Tsurugi K

更新日期：2001-11-01 00:00:00

abstract：:The mechanism of binding between thermolysin with its specific inhibitor, talopeptin (MKI), was studied kinetically with the stopped-flow method by monitoring the enhancement of tryptophan fluorescence caused by the complex formation. Only one relaxation obeying first-order kinetics was observed. The dependence of the...

journal_title：Journal of biochemistry

authors： Kitagishi K,Hiromi K

更新日期：1983-01-01 00:00:00

abstract：:When mice were exposed to 100% oxygen at a pressure of 6 atm. absolute, the animals suffered from severe convulsions. The content of gamma-aminobutyric acid (GABA) in synaptosomes was lower in the exposed animals than in unexposed ones. The exposure to high pressure oxygen produced a considerable reduction in GABA for...

journal_title：Journal of biochemistry

authors： Hori S

更新日期：1982-02-01 00:00:00

abstract：:The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...

journal_title：Journal of biochemistry

authors： Katagiri M,Murakami H,Yabusaki Y,Sugiyama T,Okamoto M,Yamano T,Ohkawa H

更新日期：1986-10-01 00:00:00

abstract：:The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release from microsomes of corn coleoptiles was investigated. Addition of micromolar concentrations of IP3 to Ca2+ loaded microsomes resulted in rapid release of 20-30% of sequestered Ca2+. Maximal and half maximal Ca2+ release occurred at 20 and 8 microM of IP3...

journal_title：Journal of biochemistry

authors： Reddy AS,Poovaiah BW

更新日期：1987-03-01 00:00:00

abstract：:3-Isopropylmalate dehydrogenase (IPMDH) is a dimeric enzyme with a strongly hydrophobic core that is composed of residues from four alpha-helices. We replaced Glu253, which is found in the hydrophobic core and is part of the subunit interface of the Bacillus subtilis (Bs) IPMDH, with several other amino acids to probe...

journal_title：Journal of biochemistry

authors： Ohkuri T,Yamagishi A

更新日期：2007-06-01 00:00:00

abstract：:Kinetic analyses of the hydrolysis reactions of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside [(GlcNAc)(2)-UMB (1), GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)(2)-UMB (4)] by ChiA and ChiB from Serratia marcescens were performed. Both enzymes released UMB from all compounds ...

journal_title：Journal of biochemistry

authors： Honda Y,Kitaoka M,Tokuyasu K,Sasaki C,Fukamizo T,Hayashi K

更新日期：2003-02-01 00:00:00

abstract：:Previously, we isolated t-cinnamoyl-D-glucose as a possible intermediate in chlorogenic acid biosynthesis from sweet potato root. The enzyme which catalyzes the formation of t-cinnamoyl-D-glucose has been purified 539-fold from sweet potato root (Ipomoea batatas Lam.) and characterized. It required UDP-glucose as a gl...

journal_title：Journal of biochemistry

authors： Shimizu T,Kojima M

更新日期：1984-01-01 00:00:00

abstract：:A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...

journal_title：Journal of biochemistry

authors： Kajiwara K,Kumazaki T,Sato E,Kanaoka Y,Ishii S

更新日期：1991-09-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect...

journal_title：Journal of biochemistry

authors： Kagiwada S,Zen R

更新日期：2003-04-01 00:00:00

abstract：:Studies were conducted to induce urinary excretion of retinol-binding protein (RBP) in the rabbit by long-term administration of cadmium, and to isolate and characterize rabbit RBP. Two rabbits were exposed to Cd at a dose of 0.8 and/or 1.5mg/kg of body weight by means of subcutaneous injections five times per week. O...

journal_title：Journal of biochemistry

authors： Muto Y,Nakanishi M,Shidoji Y

更新日期：1976-04-01 00:00:00

abstract：:Acid ceramidase (ACDase) metabolizes ceramide to sphingosine, leading to sphingosine 1-phosphate production. Reportedly, ACDase has been upregulated in prostate cancer. However, its regulatory mechanism remains unclear. LNCaP (androgen-sensitive prostate cancer cell line) but not PC3 and DU-145, (androgen-unresponsive...

journal_title：Journal of biochemistry

authors： Mizutani N,Inoue M,Omori Y,Ito H,Tamiya-Koizumi K,Takagi A,Kojima T,Nakamura M,Iwaki S,Nakatochi M,Suzuki M,Nozawa Y,Murate T

更新日期：2015-10-01 00:00:00

abstract：:Midkine (MK) is a product of a retinoic acid-responsive gene and a new heparin-binding growth/differentiation factor. The coding sequence of human MK was located on 1.5 kb DNA segment. The structure of the cloned human MK gene was determined and compared with that of the mouse gene. The coding sequence was divided int...

journal_title：Journal of biochemistry

authors： Uehara K,Matsubara S,Kadomatsu K,Tsutsui J,Muramatsu T

更新日期：1992-05-01 00:00:00

abstract：:A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...

journal_title：Journal of biochemistry

authors： Kinoshita M,Okazaki M,Kato H,Teramoto T,Matsushima T,Naito C,Oka H,Hara I

更新日期：1983-08-01 00:00:00

abstract：:Elafin was shown to be a new type of proteinase inhibitor which has an anchoring sequence. Human elafin, a potent inhibitor specific for elastase and proteinase 3, has a unique repeating sequence in its prosegment that is rich in Gln and Lys residues. The prosegment, termed "cementoin," exhibits high homology with the...

journal_title：Journal of biochemistry

authors： Nara K,Ito S,Ito T,Suzuki Y,Ghoneim MA,Tachibana S,Hirose S

更新日期：1994-03-01 00:00:00

abstract：:Mitotic kinesin Eg5 plays an important physiological role in cell division. Several small-molecule inhibitors of Eg5 are the focus of cancer therapies. Azobenzene is a photochromic compound exhibiting cis-trans isomerization upon ultraviolet (UV) and visible (VIS) light irradiation. Photochromic compounds of azobenzen...

journal_title：Journal of biochemistry

authors： Sadakane K,Alrazi IMD,Maruta S

更新日期：2018-10-01 00:00:00

abstract：:Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating) mainly catalyzes oxygenation when L-phenylalanine is used as the substrate, but oxidation when L-methionine is used as the substrate. Using [C(alpha)-H]-DL-methionine and [C(alpha)-D]-DL-methionine as substrate, the reductive half reaction of FAD co...

journal_title：Journal of biochemistry

authors： Ohta Y,Mukouyama EB,Suzuki H

更新日期：2006-03-01 00:00:00

abstract：:Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related...

journal_title：Journal of biochemistry

authors： Kato S,Tsuji M,Nakanishi Y,Suzuki S

更新日期：1980-03-01 00:00:00

abstract：:The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or ...

journal_title：Journal of biochemistry

authors： Takigawa M,Watanabe R,Ishida H,Asada A,Suzuki F

更新日期：1979-01-01 00:00:00

abstract：:The 20,000 dalton light chain (L2) was isolated from rabbit and chicken striated muscle myosins, and the Ca2+-induced conformational changes of these proteins were investigated. 1) The reaction of thiol groups of L2 with dithiobisnitrobenzoic acid (DTNB), 2) measurements of the UV difference absorption spectrum, 3) me...

journal_title：Journal of biochemistry

authors： Minowa O,Matsuda S,Yagi K

更新日期：1983-07-01 00:00:00

abstract：:To study the effect of cross-linking of membrane proteins on vesiculation of human erythrocytes by dimyristoylphosphatidylcholine (DMPC), red cells were treated with diamide at atmospheric pressure or 100 MPa and then incubated with DMPC in buffers of pH 6.5-8.5. Irrespective of buffer pH, the amount of released vesic...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Yamada S,Kimoto E

更新日期：1994-04-01 00:00:00

abstract：:FOXP2 is a member of the P subfamily of FOX transcription factors, the DNA-binding domain of which is the winged helix forkhead domain (FHD). In this work we show that the FOXP2 FHD is able to bind to various DNA sequences, including a novel sequence identified in this work, with different affinities and rates as dete...

journal_title：Journal of biochemistry

authors： Webb H,Steeb O,Blane A,Rotherham L,Aron S,Machanick P,Dirr H,Fanucchi S

更新日期：2017-07-01 00:00:00

abstract：:To investigate a possible function of plasma albumin in partitioning organic anions into bile and urine, phenolsulfonphthalein (PSP) was administered intravenously and its in vivo fate was studied in normal and analbuminemic mutant rats (NAR). No significant change in the rate of PSP disappearance was observed in bila...

journal_title：Journal of biochemistry

authors： Inoue M,Koyama H,Nagase S,Morino Y

更新日期：1986-07-01 00:00:00

abstract：:Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catal...

journal_title：Journal of biochemistry

authors： Saichana N,Tanizawa K,Pechoušek J,Novák P,Yakushi T,Toyama H,Frébortová J

更新日期：2016-01-01 00:00:00

abstract：:The calnexin homologue (Cne1p) of Saccharomyces cerevisiae was expressed in Escherichia coli to evaluate its chaperone function. The chaperone function was examined as to the effects on the suppression of thermal denaturation and the enhancement of refolding, using citrate synthase (CS) as a nonspecific chaperone subs...

journal_title：Journal of biochemistry

authors： Xu X,Kanbara K,Azakami H,Kato A

更新日期：2004-05-01 00:00:00

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis tra...

journal_title：Journal of biochemistry

authors： Itaya M,Nagata T,Shiroishi T,Fujita K,Tsuge K

更新日期：2000-11-01 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...

journal_title：Journal of biochemistry

authors： Hagiwara K,Hara H,Hanada K

更新日期：2013-02-01 00:00:00