Efficient cloning and engineering of giant DNAs in a novel Bacillus subtilis genome vector.

abstract：:The virion-associated RNA-dependent RNA polymerase of influenza virus PR8 was activated by treatment with specific non-ionic detergents, and was remarkably stimulated by exogenously added oligonucleotide primers. Among synthetic oligonucleotides of various chain lengths, the dinucleotide ApG and the trinucleotide ApGp...

journal_title：Journal of biochemistry

authors： Kawakami K,Ishihama A,Ohtsuka E,Tanaka T,Takashima H,Ikehara M

更新日期：1981-06-01 00:00:00

abstract：:Chemical modification of gamma-carboxyglutamic acid (Gla) residues in human prothrombin to gamma-methyleneglutamic acid (gamma-MGlu) residues elicited a conformation similar, if not identical, to that of des-gamma-carboxy prothrombin or PIVKA-II, i.e., prothrombin molecules induced by vitamin K antagonists or vitamin ...

journal_title：Journal of biochemistry

authors： Sugo T,Watanabe K,Naraki T,Matsuda M

更新日期：1990-09-01 00:00:00

abstract：:Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

journal_title：Journal of biochemistry

authors： Mima J,Suzuki H,Takahashi M,Hayashi R

更新日期：2002-12-01 00:00:00

abstract：:The transcription and replication of influenza RNA can be studied in vitro by the reconstitution of functional ribonucleoprotein (RNP) complex from viral core proteins including the RNA polymerase (complex of three P protein subunits) and nucleoprotein (NP), and model templates. Here, two different core protein prepar...

journal_title：Journal of biochemistry

authors： Seong BL,Kobayashi M,Nagata K,Brownlee GG,Ishihama A

更新日期：1992-04-01 00:00:00

abstract：:Inosine triphosphate (ITP) does not serve as a substrate for myosin light-chain kinase from gizzard muscle. That is to say, myosin light-chain is not phosphorylated in ITP media. Nevertheless, at pH 6.8, 1 mM or 5 mM ITP induces superprecipitation of skeletal acto-gizzard myosin. The ITP-induced superprecipitation occ...

journal_title：Journal of biochemistry

authors： Onishi H,Watanabe S

更新日期：1979-08-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:It was found that the absorption spectrum of beta-naphthyl triphosphate is different from that of beta-naphthyl diphosphate in the range 290-335 nm. Thus, beta-naphthyl triphosphate hydrolysis by heavy meromyosin can be recorded continuously as a function of time by means of a spectrophotometer. By analyzing the time ...

journal_title：Journal of biochemistry

authors： Kuwajima T,Kagawa H,Asai H

更新日期：1975-11-01 00:00:00

abstract：:Gastric cancer (GC) is one of the most common cancers in gastrointestinal malignant tumours. Long non-coding RNAs were widely reported to play a significant role in the regulation of occurrence or development of tumours. Bioinformatics analysis and a wide range of experiments were conducted to explore the expression s...

journal_title：Journal of biochemistry

authors： Xin H,Yan Z,Cao J

更新日期：2020-11-01 00:00:00

abstract：:Recombinant mouse Dnmt3a and Dnmt3b were expressed in sf9 cells and purified to near homogeneity. The purified Dnmt3a and Dnmt3b gave specific activities of 1.8 +/- 0.3 and 1.3 +/- 0.1 mol/h/mol enzyme towards poly(dGdC)-poly(dGdC), respectively, which were the highest among those reported. Dnmt3a or Dnmt3b showed sim...

journal_title：Journal of biochemistry

authors： Suetake I,Miyazaki J,Murakami C,Takeshima H,Tajima S

更新日期：2003-06-01 00:00:00

abstract：:The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous w...

journal_title：Journal of biochemistry

authors： Yoon SS,Park SH,Kim YC,Shin M,Chong CK,Choi JD

更新日期：2008-10-01 00:00:00

abstract：:Histidine and its derivatives increased rabbit muscle fructose 1,6-bisphosphatase activity at neutral pH with positive cooperativity. In the presence of histidine and carnosine the optimum pH shifted from pH 8.0 to 7.4. The cooperative response of the enzyme to AMP and fructose 1,6-bisphosphate was observed in the pre...

journal_title：Journal of biochemistry

authors： Ikeda T,Kimura K,Hama T,Tamaki N

更新日期：1980-01-01 00:00:00

abstract：:Soluble guanylate cyclase (sGC) consisting of two different subunits (alpha: Mr = 74,000, beta: Mr = 69,000) was purified more than 12,000-fold in terms of specific activity from the supernatant of bovine lung homogenates and characterized. The heme content determined with the pyridine hemochromogen method and Bradfor...

journal_title：Journal of biochemistry

authors： Tomita T,Tsuyama S,Imai Y,Kitagawa T

更新日期：1997-09-01 00:00:00

abstract：:When the body temperature of rats was brought to 42 degrees C, four heat-shock proteins, with molecular weights of 70,000, 71,000, 85,000, and 100,000 (hsp 70, hsp 71, hsp 85, and hsp 100, respectively), were induced in various tissues of the rats. The hsp 70 was strongly induced by hyperthermia, and its accumulation ...

journal_title：Journal of biochemistry

authors： Fujio N,Hatayama T,Kinoshita H,Yukioka M

更新日期：1987-01-01 00:00:00

abstract：:We investigated the effects of aging and denervation on the gene expression of uncoupling proteins (UCPs) in slow-twitch soleus and fast-twitch gastrocnemius muscles. In a comparison between the control limbs of 6- and 24-month-old rats, the mRNA levels of UCP3, heart-type fatty acid binding protein (HFABP), and gluco...

journal_title：Journal of biochemistry

authors： Kontani Y,Wang Z,Furuyama T,Sato Y,Mori N,Yamashita H

更新日期：2002-08-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:Histidinoalanine, a cross-linking component of connective tissue proteins, was detected in the acid hydrolysate of human urine. The concentrations in urines from newborn babies, children, and adults were 1.33 +/- 0.27, 0.77 +/- 0.23, and 0.89 +/- 0.33 nmol/mg creatinine, respectively. Possible origins of urinary histi...

journal_title：Journal of biochemistry

authors： Fujimoto D

更新日期：1986-09-01 00:00:00

abstract：:Synthesis and degradation of glycerophospholipids in HL-60 cells and retinoic acid (RA)-treated HL-60 cells were examined. The synthesis of each subclass of ethanolamine-containing glycerophospholipids was extremely suppressed in RA-treated HL-60 cells, while that of other glycerophospholipids was not seriously affect...

journal_title：Journal of biochemistry

authors： Naito M,Kudo I,Nakagawa Y,Waku K,Nojiri H,Saito M,Inoue K

更新日期：1991-10-01 00:00:00

abstract：:Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

journal_title：Journal of biochemistry

authors： Yamashita T,Kawakita M

更新日期：1992-02-01 00:00:00

abstract：:We found that transducing phages carrying the gal or bio regions of the Escherichia coli genome were formed during in vitro packaging of endogenous lambda DNA. Structural analysis of the transducing phage genomes indicated that they were formed by abnormal excision of lambda prophage. Formation of transducing phages w...

journal_title：Journal of biochemistry

authors： Tomono M,Shiozaki M,Ikeda H

更新日期：1989-03-01 00:00:00

abstract：:The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

journal_title：Journal of biochemistry

authors： Ohta M,Matsumoto H,Ishii K,Ueno Y

更新日期：1978-09-01 00:00:00

abstract：:Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels o...

journal_title：Journal of biochemistry

authors： Hidaka M,Fushinobu S,Honda Y,Wakagi T,Shoun H,Kitaoka M

更新日期：2010-02-01 00:00:00

abstract：:In the nucleus of eukaryotic cells, chromatin is tethered to the nuclear envelope (NE), wherein inner nuclear membrane proteins (INMPs) play major roles. However, in Xenopus blastula, chromatin tethering to the NE depends on nuclear filamentous actin that develops in a blastula-specific manner. To investigate whether ...

journal_title：Journal of biochemistry

authors： Oda H,Kato S,Ohsumi K,Iwabuchi M

更新日期：2020-11-10 00:00:00

abstract：:Chloroquine myopathy is a drug poisoning disease involving rimmed vacuole formation. By Western blot analysis, we investigated posttranslational modification of LC3 in cultured cells with a high concentration of chloroquine, and found that the autophagosome membrane-bound form of LC3 increased dose-dependently. We als...

journal_title：Journal of biochemistry

authors： Suzuki T,Nakagawa M,Yoshikawa A,Sasagawa N,Yoshimori T,Ohsumi Y,Nishino I,Ishiura S,Nonaka I

更新日期：2002-05-01 00:00:00

abstract：:NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

journal_title：Journal of biochemistry

authors： Shioi Y,Takamiya K,Nishimura M

更新日期：1976-02-01 00:00:00

abstract：:The mitochondrion is an essential organelle for a wide range of cellular processes, including energy production, metabolism, signal transduction and cell death. To execute these functions, mitochondria regulate their size, number, morphology and distribution in cells via mitochondrial division and fusion. In addition,...

journal_title：Journal of biochemistry

authors： Murata D,Arai K,Iijima M,Sesaki H

更新日期：2020-03-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Oxygen-binding to haemocyanin (Hc) is generally an exothermic process, with overall enthalphy of oxygenation varying from species to species. A number of crustacean Hcs showed a null or reduced enthalphy of oxygenation, among others, the anomuran Pagurus bernhardus and Paralithodes camtscaticae possess a completely te...

journal_title：Journal of biochemistry

authors： Podda G,Manconi B,Olianas A,Pellegrini M,Messana I,Mura M,Castagnola M,Giardina B,Sanna MT

更新日期：2008-02-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00