Effects of aging and denervation on the expression of uncoupling proteins in slow- and fast-twitch muscles of rats.

abstract：:Under conditions where nM level of calmodulin was able to show full activation of myosin light chain kinase and cyclic-nucleotide phosphodiesterase, the fragments of calmodulin at concentrations as high as 20 microM failed to activate these enzymes in the presence of Ca2+. The fragments tested were Ala1-Lys75 (F12), A...

journal_title：Journal of biochemistry

authors： Minowa O,Yazawa M,Sobue K,Ito K,Yagi K

更新日期：1988-03-01 00:00:00

abstract：:Specific and high titer antisera against 4-O-methylglucuronic acid-containing glycosphingolipid (Lipid IV) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits. The antisera were found to agglutinate spermatozoa of three fresh-water bivalves, H. schlegelii, Anodonta woodiana, and C...

journal_title：Journal of biochemistry

authors： Sugita M,Inoue T,Itasaka O,Hori T

更新日期：1984-03-01 00:00:00

abstract：:PKNalpha is a fatty acid- and Rho-activated serine/threonine protein kinase having a catalytic domain homologous to members of the protein kinase C family. Recently it was reported that PKNalpha is involved in the p38 mitogen-activated protein kinase (MAPK) signaling pathway. To date, however, how PKNalpha regulates t...

journal_title：Journal of biochemistry

authors： Takahashi M,Gotoh Y,Isagawa T,Nishimura T,Goyama E,Kim HS,Mukai H,Ono Y

更新日期：2003-02-01 00:00:00

abstract：:A fifth and new DFP-sensitive alkaline proteinase E, with strong esterase activity toward Ac-(Ala)3-OMe was found in pronase, a protease mixture from St. griseus K-1. Proteinase E was shown to be different from the elastase [EC 3.4.21.11]-like enzyme or subtilisin [EC 3.4.21.14]like enzyme, and alkaline proteinase A, ...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1976-05-01 00:00:00

abstract：:The conditions for the preparation of the ferricytochrome a-ferrocytochrome a3-carbon monoxide complex (a3+, a3(2)+CO) of cytochrome oxidase [EC 1.9.3.1] by the ferricyanide-reoxidation method and some properties of the prepared complex were studied. The addition of a small volume of concentrated ferricyanide solution...

journal_title：Journal of biochemistry

authors： Horie S,Watanabe T,Ave K

更新日期：1983-04-01 00:00:00

abstract：:A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

journal_title：Journal of biochemistry

authors： Ohnishi M,Yamashita T,Hiromi K

更新日期：1977-01-01 00:00:00

abstract：:Methioninase of Pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. The purified enzyme had an S20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. A break in the Arrhen...

journal_title：Journal of biochemistry

authors： Ito S,Nakamura T,Eguchi Y

更新日期：1976-06-01 00:00:00

abstract：:Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

journal_title：Journal of biochemistry

authors： Yamashita T,Kawakita M

更新日期：1992-02-01 00:00:00

abstract：:Bromoacetamide derivatives having n-alkyl substituents (BrCH2CONH(CH2)nH, 1-n) and carboxyalkyl substituents (BrCH2CONH(CH2)nCOOH, 2-n) react with His-15 in lysozyme exclusively at N epsilon 2 at pH 5.5 and 40 degree C. Kinetic studies suggest that lysozyme has a small hydrophobic pocket that binds these reagents in t...

journal_title：Journal of biochemistry

authors： Yamada H,Uozumi F,Ishikawa A,Imoto T

更新日期：1984-02-01 00:00:00

abstract：:G-actin freed from exogenous ATP was added to the pieces of isolated acrosomal actin bundles from horseshoe crab sperm to form filaments as reported earlier (Tilney, L.G., Bonder, E.M., & DeRosier, D.J. (1981) J. Cell Biol. 90, 485-494). The growth of a filament was far more rapid at one end (the preferred end) than t...

journal_title：Journal of biochemistry

authors： Mabuchi I

更新日期：1983-10-01 00:00:00

abstract：:In our previous study, the sphingosine-like immunosuppressant ISP-1 was shown to induce apoptosis in the mouse cytotoxic T cell line CTLL-2. In this study, we characterized the ISP-1-induced apoptotic pathway. Although caspase-3-like protease activity increases concomitantly with ISP-1-induced apoptosis in CTLL-2 cell...

journal_title：Journal of biochemistry

authors： Yamaji T,Nakamura S,Takematsu H,Kawasaki T,Kozutsumi Y

更新日期：2001-04-01 00:00:00

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:The DNA chain elongation mechanisms of mouse DNA polymerases alpha and beta have been analyzed by using denatured DNA with a (dT)n block at the 3'-end as a template in combination with RNA ((rA)12-20)primer. The (rA)12-20-primed DNA product synthesized by DNA polymerase alpha was 3-5 s in size even after prolonged rea...

journal_title：Journal of biochemistry

authors： Matsukage A,Nishizawa M,Takahashi T,Hozumi T

更新日期：1980-12-01 00:00:00

abstract：:Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV...

journal_title：Journal of biochemistry

authors： Asano K,Suzuki M,Handa S,Oshima M

更新日期：1991-01-01 00:00:00

abstract：:Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...

journal_title：Journal of biochemistry

authors： Sugiyama H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:Equilibrium gel permeation chromatography was employed to determine the ability of heparin to form complexes with thrombin and antithrombin III. In the eluate from a Sephacryl S-200 column, heparin caused a peak and then a trough in the fluorescence of 48 nM antithrombin III or 63 nM thrombin. The peak-heights with kn...

journal_title：Journal of biochemistry

authors： Oshima G,Uchiyama H,Nagasawa K

更新日期：1984-10-01 00:00:00

abstract：:Terf/TRIM17 is a tripartite motif protein that has been originally isolated from testis. Terf has been characterized to exhibit an E3 ubiquitin ligase activity and to undergo self-ubiquitination. The cellular function of terf and its substrates, however, remain elusive. In the present study, we performed a yeast two-h...

journal_title：Journal of biochemistry

authors： Endo H,Ikeda K,Urano T,Horie-Inoue K,Inoue S

更新日期：2012-02-01 00:00:00

abstract：:The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release from microsomes of corn coleoptiles was investigated. Addition of micromolar concentrations of IP3 to Ca2+ loaded microsomes resulted in rapid release of 20-30% of sequestered Ca2+. Maximal and half maximal Ca2+ release occurred at 20 and 8 microM of IP3...

journal_title：Journal of biochemistry

authors： Reddy AS,Poovaiah BW

更新日期：1987-03-01 00:00:00

abstract：:One of the 2 main hemoglobins of the sea snake Pelamis platurus, (the Yellow-bellied sea snake) comprising about 70% of the total hemoglobin, was separated by DEAE-Sephadex column chromatography. From results of gel filtration and iron content determination, both intact sea snake hemoglobin, and the isolated major hem...

journal_title：Journal of biochemistry

authors： Liu CS

更新日期：1975-07-01 00:00:00

abstract：:Using an F-actin affinity column, a 60 kDa fragment of a 71 kDa F-actin-binding protein was partially purified from Tetrahymena pyriformis. After digestion of the 60 kDa fragment with cyanogen bromide, the N-terminal 21-amino acid sequence of one of the resulting peptides was found to show sequence similarity to a reg...

journal_title：Journal of biochemistry

authors： Watanabe A,Kurasawa Y,Watanabe Y,Numata O

更新日期：1998-04-01 00:00:00

abstract：:Proteoglycan biosynthesis in developing chicken corneas was studied. Corneas free of scleral rims were prepared from 5- to 18-day-old chick embryos and labeled in vitro with [3H]glucosamine and [35S]sulfate. Then, the labeled medium and corneal proteoglycans were analyzed. Day 5 corneas synthesized mainly chondroitin ...

journal_title：Journal of biochemistry

authors： Nakazawa K,Suzuki S,Wada K,Nakazawa K

更新日期：1995-04-01 00:00:00

abstract：:One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

journal_title：Journal of biochemistry

authors： Andoh R,Yamamoto T

更新日期：1985-03-01 00:00:00

abstract：:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hem...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Matsumoto M,Kimoto E

更新日期：1995-10-01 00:00:00

abstract：:A glutathione S-transferase isozyme which is absent in normal rat liver has been isolated from the hereditary hyperbilirubinuria rat liver cytosol. The enzyme was purified to apparent homogeneity by GSH-affinity chromatography and HPLC on CM-Sepharose CL-6B. It is a heterodimer of two non-identical subunits, i.e., sub...

journal_title：Journal of biochemistry

authors： Igarashi T,Tsuchiya T,Satoh T

更新日期：1991-01-01 00:00:00

abstract：:Substrate specificities of phospholipases C[EC 3.1.4.3] from Clostridium novyi, Clostridium perfringens, Bacillus cereus, and Pseudomonas aureofaciens were studied under the same conditions. Phospholipases C from Clostridium novyi and Bacillus cereus show wide substrate specificities while those of Clostridium perfrin...

journal_title：Journal of biochemistry

authors： Taguchi R,Ikezawa H

更新日期：1977-11-01 00:00:00

abstract：:We previously reported the utilization of antisense RNA in the development of a novel immune system against RNA coliphage SP proliferation (Hirashima et al. [1986] Proc. Natl. Acad. Sci. U.S. 83, 7726-7730). We attempted to determine the most effective (i.e., those eliciting antiviral activity) sequences for targeting...

journal_title：Journal of biochemistry

authors： Hirashima A,Sawaki S,Mizuno T,Houba-Herin N,Inouye M

更新日期：1989-07-01 00:00:00

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:Yeast mitochondrial ATP synthase has three regulatory proteins, ATPase inhibitor, 9K protein, and 15K protein. The 9K protein binds directly to purified F1-ATPase, as does the ATPase inhibitor, but the 15K protein does not [Hashimoto, T. et al. (1987) J. Biochem. 102, 685-692]. In the present study, we found that 15K ...

journal_title：Journal of biochemistry

authors： Hashimoto T,Yoshida Y,Tagawa K

更新日期：1990-07-01 00:00:00

abstract：:Although the depletion of reduced glutathione (GSH) has been observed in a variety of apoptotic systems, little is known about the mechanism of GSH depletion. In this study we used polarized MDCK cells to study the GSH flux during ricin-induced apoptosis. Here we report that the specific accumulation of GSH occurred i...

journal_title：Journal of biochemistry

authors： Oda T,Sadakata N,Komatsu N,Muramatsu T

更新日期：1999-10-01 00:00:00

abstract：:Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...

journal_title：Journal of biochemistry

authors： Okubo T,Yanai N,Watanabe S,Arai Ki,Obinata M

更新日期：2000-04-01 00:00:00