Abstract:
:Using an F-actin affinity column, a 60 kDa fragment of a 71 kDa F-actin-binding protein was partially purified from Tetrahymena pyriformis. After digestion of the 60 kDa fragment with cyanogen bromide, the N-terminal 21-amino acid sequence of one of the resulting peptides was found to show sequence similarity to a region near the actin-binding site (amino acid residues 260-281) of yeast fimbrin. An antibody prepared against a synthesized 21-mer oligopeptide reacted with the 71 kDa proteins in T. pyriformis and T. thermophila cell extracts, suggesting that the 60 kDa fragment was produced from the 71 kDa protein through partial digestion occurring during isolation. The 60 kDa fragment bound to Tetrahymena F-actin as well as to rabbit skeletal muscle F-actin, and induced the bundling of Tetrahymena F-actin. Indirect immunofluorescence revealed colocalization of the 71 kDa protein and actin in the oral apparatus and the deep fiber bundles in T. pyriformis. On the other hand, in T. thermophila, the 71 kDa protein was localized in the oral apparatus and the contractile vacuole pores during the interphase. During cytokinesis, the 71 kDa protein was localized in the division furrow. Therefore, the 71 kDa protein seems to associate with the actin cytoskeleton, and to regulate the actin filament organization during phagocytosis and cytokinesis in Tetrahymena.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Watanabe A,Kurasawa Y,Watanabe Y,Numata Odoi
10.1093/oxfordjournals.jbchem.a021980subject
Has Abstractpub_date
1998-04-01 00:00:00pages
607-13issue
4eissn
0021-924Xissn
1756-2651journal_volume
123pub_type
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1982-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022539
更新日期:1999-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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更新日期:2001-01-01 00:00:00
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更新日期:2019-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132979
更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1980-11-01 00:00:00
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更新日期:2017-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134560
更新日期:1983-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1986-02-01 00:00:00
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
abstract::Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00