Studies on silk fibroin of Bombyx mori. I. Fractionation of fibroin prepared from the posterior silk gland.

abstract：:Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC 4.1.2.13] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

journal_title：Journal of biochemistry

authors： Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

更新日期：1991-04-01 00:00:00

abstract：:Reverse transcriptases (RTs) from Moloney murine leukaemia virus (MMLV) and avian myeloblastosis virus (AMV) contain all the fingers, palm, thumb, connection and RNase H domains. The fingers, palm and thumb domains are thought to be involved in the reverse transcription activity, and the RNase H domain is in the RNase...

journal_title：Journal of biochemistry

authors： Yasukawa K,Mizuno M,Inouye K

更新日期：2009-03-01 00:00:00

abstract：:To investigate the role of carboxyl groups of glucoamylases [EC 3.2.1.3] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

journal_title：Journal of biochemistry

authors： Iwama M,Ohtsuki R,Takahashi T,Irie M

更新日期：1984-08-01 00:00:00

abstract：:Dupuytren's disease is a palmar fibromatosis associated with changes in fibroblast activity that also affect the metabolism of extracellular matrix components. In contrast to disease connected alterations in collagen and non-collagenous glycoproteins (mainly fibronectin), the metabolism of proteoglycans, being glycosa...

journal_title：Journal of biochemistry

authors： Koźma EM,Olczyk K,Wisowski G,Głowacki A,Bobiński R

更新日期：2005-04-01 00:00:00

abstract：:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamo-Yamada F,Cho S,Sugano M

更新日期：1980-05-01 00:00:00

abstract：:Cytochrome c oxidase from Thiobacillus ferrooxidans was purified to homogeneity and some of its properties were studied. The oxidase was solubilized with n-octyl-beta-D-thioglucoside (OTG) under acidic conditions (pH 4.0) and purified by one step of ion-exchange chromatography with a CM-Toyopearl column. The absorptio...

journal_title：Journal of biochemistry

authors： Kai M,Yano T,Tamegai H,Fukumori Y,Yamanaka T

更新日期：1992-12-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a class of bioactive lipid molecules in animal tissues, including the endocannabinoid anandamide and the anti-inflammatory substance N-palmitoylethanolamine. Enzymatic hydrolysis of NAEs is considered to be an important step to regulate their endogenous levels. Lysosomal NAE-hydrolysing ...

journal_title：Journal of biochemistry

authors： Wang J,Zhao LY,Uyama T,Tsuboi K,Wu XX,Kakehi Y,Ueda N

更新日期：2008-11-01 00:00:00

abstract：:We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 +/- 3 ng/mg; esophagus, 23 +/- 8 ng/mg; stomach, 18 +/- 6 ng...

journal_title：Journal of biochemistry

authors： Sato M,Nishi N,Shoji H,Kumagai M,Imaizumi T,Hata Y,Hirashima M,Suzuki S,Nakamura T

更新日期：2002-02-01 00:00:00

abstract：:Specific palindromic sequences in synthetic oligonucleotides are required to induce IFN and augment IFN-mediated natural killer activity. To study the mechanism of IFN induction by oligonucleotides containing palindromic sequences, we investigated the possible target molecules of the oligonucleotides. Oligo-1, a 30mer...

journal_title：Journal of biochemistry

authors： Kimura Y,Sonehara K,Kuramoto E,Makino T,Yamamoto S,Yamamoto T,Kataoka T,Tokunaga T

更新日期：1994-11-01 00:00:00

abstract：:The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned and studied the biochemical properties of plant-type ferredoxin (Fd) and Fd-NADP+ reductase (FNR), a redox system that p...

journal_title：Journal of biochemistry

authors： Kimata-Ariga Y,Kurisu G,Kusunoki M,Aoki S,Sato D,Kobayashi T,Kita K,Horii T,Hase T

更新日期：2007-03-01 00:00:00

abstract：:The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

journal_title：Journal of biochemistry

authors： Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

更新日期：2000-07-01 00:00:00

abstract：:Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1981-09-01 00:00:00

abstract：:Glycerol-treated muscle fiber bundles were fixed at their rest length in 50 mM KC1, 2 mM MgC1(2), and 10 micron CaC1(2) at pH 7.8 and 0 degrees C in the presence of sufficient amounts of ATP, creatine kinase, and creatine phosphate. The fiber bundles were stretched linearly with time for 0.3 s at a constant amplitude,...

journal_title：Journal of biochemistry

authors： Arata T,Mukohata Y,Tonomura Y

更新日期：1978-10-01 00:00:00

abstract：:Phospholipase A2 (PLA2) has been purified to homogeneity from human arthritic synovial fluid. The activity resolved into multiple peaks by preparative HPLC. The most abundant peak (A) was present in synovial fluid from patients with rheumatoid arthritis, osteoarthritis, and psoriatic arthritis. A second major peak (B)...

journal_title：Journal of biochemistry

authors： Seilhamer JJ,Plant S,Pruzanski W,Schilling J,Stefanski E,Vadas P,Johnson LK

更新日期：1989-07-01 00:00:00

abstract：:Caenorhabditis elegans HMG-5, which is encoded by F45E4.9, contains two high mobility group (HMG) box domains and shows sequence similarity with mammalian mitochondrial transcription factor A (TFAM). In this study, using soaking RNA interference, we found that knockdown of HMG-5 reduced the amount of mtDNA in P0 herma...

journal_title：Journal of biochemistry

authors： Sumitani M,Kasashima K,Matsugi J,Endo H

更新日期：2011-05-01 00:00:00

abstract：:Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. The purified enzyme was very labile when incubated either at 37 degrees C or 0 degrees C, and lost its ac...

journal_title：Journal of biochemistry

authors： Natori Y,Karasawa K,Arai H,Tamori-Natori Y,Nojima S

更新日期：1983-02-01 00:00:00

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:We prepared human rheumatoid arthritic synovial fluid phospholipase A2 (PLA2) [EC 3.1.1.4] from insect cells infected with a recombinant baculovirus. The PLA2 DNA was designed, changing the original codons to those used frequently in the polyhedrin gene. Sixteen oligo-deoxynucleotides ranging from 40 to 70 nucleotides...

journal_title：Journal of biochemistry

authors： Kawauchi Y,Takasaki J,Matsuura Y,Masuho Y

更新日期：1994-07-01 00:00:00

abstract：:We found functionally active thrombomodulin in human platelets (60 +/- 18 molecules per platelet). Protein C appeared not to be activated by thrombin with gel-filtered platelets. However, the activation of protein C by thrombin was accelerated by thrombin-stimulated and washed platelets. This cofactor activity of the ...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hayashi T,Kosaka Y

更新日期：1988-10-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:Type A atrial natriuretic peptide (ANP) receptor was demonstrated to be present as a tetramer in the bovine adrenal cortex. Type A ANP receptor is composed of two functional domains, namely extracellular ANP-binding and cytoplasmic guanylate cyclase domains, and generally considered to be present as a single polypepti...

journal_title：Journal of biochemistry

authors： Iwata T,Uchida-Mizuno K,Katafuchi T,Ito T,Hagiwara H,Hirose S

更新日期：1991-07-01 00:00:00

abstract：:A new multifunctional protein kinase, which normally exists as an inactive form in the soluble fraction in mammalian tissues, attaches to membranes to exhibit full enzymatic activity. A low concentration of Ca2+ is absolutely necessary for this activation. This process is reversible. cAMP shows no effect. The active f...

journal_title：Journal of biochemistry

authors： Takai Y,Kishimoto A,Iwasa Y,Kawahara Y,Mori T,Nishizuka Y,Tamura A,Fujii T

更新日期：1979-08-01 00:00:00

abstract：:The betagamma subunits of G proteins were coimmunoprecipitated with antibodies against various alpha subunits, and analyzed by silver stain and immunoblotting with conventional transfer procedure and membrane-blocking buffer containing 2% BSA. Multiple isoforms of gamma were coimmunoprecipitated with no significant di...

journal_title：Journal of biochemistry

authors： Ueda H,Morishita R,Katoh-Semba R,Kato K,Asano T

更新日期：1998-11-01 00:00:00

abstract：:Aspartic proteases of human malarial parasites are thought to play key roles in essential pathways of merozoite release, invasion and host cell hemoglobin degradation during the intraerythrocytic stages of their life cycle. Therefore, we have purified and characterized Plasmodium vivax aspartic protease, to determine ...

journal_title：Journal of biochemistry

authors： Sharma A,Eapen A,Subbarao SK

更新日期：2005-07-01 00:00:00

abstract：:It was found that the absorption spectrum of beta-naphthyl triphosphate is different from that of beta-naphthyl diphosphate in the range 290-335 nm. Thus, beta-naphthyl triphosphate hydrolysis by heavy meromyosin can be recorded continuously as a function of time by means of a spectrophotometer. By analyzing the time ...

journal_title：Journal of biochemistry

authors： Kuwajima T,Kagawa H,Asai H

更新日期：1975-11-01 00:00:00

abstract：:1. A major glucoamylase [EC 3.2.1.3] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

journal_title：Journal of biochemistry

authors： Takahashi T,Inokuchi N,Irie M

更新日期：1981-01-01 00:00:00

abstract：:We have been interested in partially folded proteins with marginal stability and activity, because they have a potential to be mature proteins by artificial evolution. A module is defined as a contiguous peptide chain forming a compact region in a globular protein. Modules may be used as building blocks to create part...

journal_title：Journal of biochemistry

authors： Tsuji T,Nagata T,Yanagawa H

更新日期：2008-10-01 00:00:00

abstract：:A simple, sensitive, and accurate method for determination of cholestanol in plasma is described. In this method a fixed amount of cholestane is added to 1 ml of plasma as an internal standard and steroids are saponified and extracted with n-hexane. The amounts of cholestanol and cholesterol are determined by mass fra...

journal_title：Journal of biochemistry

authors： Seyama Y,Ichikawa K,Yamakawa T

更新日期：1976-08-01 00:00:00

abstract：:To analyze the processing of extracellular enzymes of Bacillus subtilis, an NH2-terminally extended hybrid alpha-amylase [pTUBE638-alpha-amylase (E24)] was purified from the periplasm of E. coli(pTUBE638) as the substrate for the in vitro processing reaction, in which a 21-amino-acid extra-peptide was added at the NH2...

journal_title：Journal of biochemistry

authors： Itoh Y,Sumi M,Nakamura K,Yamane K

更新日期：1990-12-01 00:00:00

abstract：:The oleate (delta 9-18:1)/cis-vaccenate (delta 11-18:1) ratios in phospholipids increased in the order of normal liver, host liver and hepatoma in rats. The amount of oleate increased in phospholipids and decreased in triacylglycerol in the same order, whereas the distributions of cis-vaccenate among the major lipid c...

journal_title：Journal of biochemistry

authors： Hori T,Okuyama H

更新日期：1987-05-01 00:00:00