Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus.

abstract：:Ca(2+)/calmodulin-dependent protein kinases (CaM-kinases) I and IV are activated upon phosphorylation of their Thr(177) and Thr(196), respectively, by the upstream Ca(2+)/calmodulin-dependent protein kinases CaM-kinase kinase alpha and beta, and deactivated upon dephosphorylation by protein phosphatases such as CaM-ki...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：2001-10-01 00:00:00

abstract：:When mice were exposed to 100% oxygen at a pressure of 6 atm. absolute, the animals suffered from severe convulsions. The content of gamma-aminobutyric acid (GABA) in synaptosomes was lower in the exposed animals than in unexposed ones. The exposure to high pressure oxygen produced a considerable reduction in GABA for...

journal_title：Journal of biochemistry

authors： Hori S

更新日期：1982-02-01 00:00:00

abstract：:1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z...

journal_title：Journal of biochemistry

authors： Muguruma M,Muguruma Y,Fukazawa T

更新日期：1980-07-01 00:00:00

abstract：:The present data describe the mechanism of the inhibitory effects of human plasma apolipoproteins A-I and A-II on hydrolysis of triglyceride catalyzed by hepatic triglyceride lipase using a substrate of triolein particles stabilized with gum arabic in vitro. The experimental data could well be described by a model in ...

journal_title：Journal of biochemistry

authors： Kubo M,Matsuzawa Y,Yokoyama S,Tajima S,Ishikawa K,Yamamoto A,Tarui S

更新日期：1982-09-01 00:00:00

abstract：:NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

journal_title：Journal of biochemistry

authors： Shioi Y,Takamiya K,Nishimura M

更新日期：1976-02-01 00:00:00

abstract：:The plasma membrane, the outermost surface of eukaryotic cells, contains various substructures, such as protrusions or invaginations, which are associated with diverse functions, including endocytosis and cell migration. These structures of the plasma membrane can be considered as tubules or inverted tubules (protrusi...

journal_title：Journal of biochemistry

authors： Suetsugu S

更新日期：2010-07-01 00:00:00

abstract：:Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

journal_title：Journal of biochemistry

authors： Nakanishi N,Takeuchi F,Tsubaki M

更新日期：2007-11-01 00:00:00

abstract：:NADPH oxidase was induced in HL-60 human promyelocytic leukemia cells when these cells were treated with 10(-7) M 1,25-dihydroxyvitamin D3 (VD3) for 4 days. The treated cells were disrupted by sonication, and the postnuclear fraction was separated into 48,000 X g supernatant (cytosol) and precipitate (membrane) fracti...

journal_title：Journal of biochemistry

authors： Nozawa R,Kato H,Yokota T

更新日期：1988-01-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

journal_title：Journal of biochemistry

authors： Morita T,Huruta T,Ashiuchi M,Yagi T

更新日期：2002-10-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:The interactions of porcine alpha2-macroglobulin (alpha2M) with native proteinases, their zymogens and the chemically-modified enzymes were compared. The alpha2M did not bind to chymotrypsinogen, or to most of the chemically modified derivatives of alpha-chymotrypsin, trypsinogen, DIP- and PMS-trypsins, but it could i...

journal_title：Journal of biochemistry

authors： Tsuru D,Kado K,Fujiwara K,Tmimatsu M,Ogita K

更新日期：1978-05-01 00:00:00

abstract：:One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

journal_title：Journal of biochemistry

authors： Andoh R,Yamamoto T

更新日期：1985-03-01 00:00:00

abstract：:I attempted to isolate synaptic vesicles by gel filtration. The rat brain synaptic vesicles in a synaptosomal lysate were collected by ammonium sulfate salting-out and fractionated on a Sephacryl S-500 with a mean exclusion size of 200 nm. Peak I at the void volume contained large vesicular membranes and coated vesicl...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：2003-04-01 00:00:00

abstract：:The dopamine (DA)-translocating mechanism of synaptic vesicles isolated from rat brain has been studied in the presence of an artificially imposed delta pH on the vesicle membranes (acidic inside with respect to the external medium) without the aid of ATP-Mg2+. Under the experimental conditions, [3H]DA uptake by the s...

journal_title：Journal of biochemistry

authors： Tsudzuki T

更新日期：1984-06-01 00:00:00

abstract：:Administration of an excessive dose of Bacillus subtilis alpha-amylase [EC 3.2.1.1, alpha-1,4-glucan 4-glucanohydrolase] (BalphaA) induced the production of non-precipitating (non-ppt) IgG2 antibody in guinea pigs, whereas immunization with a normal dose produced precipitating (ppt) IgG1 and IgG2 antibodies. The non-p...

journal_title：Journal of biochemistry

authors： Mori N,Nakamura T,Koyama J

更新日期：1977-07-01 00:00:00

abstract：:Rhodopsin from the bovine rod outer segment contains a covalently linked carbohydrate moiety (Heller, J. & Lawrence, M.A. (1973) Biochemistry 9, 864--868). We studied the location of this carbohydrate moiety on the disk membrane by using ferritin-conjugated concanavalin A and concanavalin A labelled with fluorescein i...

journal_title：Journal of biochemistry

authors： Nakano T,Ikai A,Nishigai M,Noda H

更新日期：1979-05-01 00:00:00

abstract：:Monoclonal antibodies were raised against a synthetic peptide (43 amino acid residues) that corresponds to the complete profragment of human prorenin. Seven monoclonal antibodies were chosen for further characterization. Two antibodies, 2-X-C1 and 4-X-E1, reacted with the middle region and C-terminus of the profragmen...

journal_title：Journal of biochemistry

authors： Ishizuka Y,Iizumi S,Akiyama F,Hori H,Poorman RA,Lobl TJ,Murakami K

更新日期：1989-09-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 +/- 3 ng/mg; esophagus, 23 +/- 8 ng/mg; stomach, 18 +/- 6 ng...

journal_title：Journal of biochemistry

authors： Sato M,Nishi N,Shoji H,Kumagai M,Imaizumi T,Hata Y,Hirashima M,Suzuki S,Nakamura T

更新日期：2002-02-01 00:00:00

abstract：:We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole...

journal_title：Journal of biochemistry

authors： Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo T

更新日期：2007-04-01 00:00:00

abstract：:We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

journal_title：Journal of biochemistry

authors： Yamamoto K

更新日期：1993-12-01 00:00:00

abstract：:"DNase binding protein" of 43K daltons as determined by SDS-polyacrylamide gel electrophoresis, was purified from the 0.1 M KCl-soluble (non-structural) fraction of chicken skeletal muscle. The protein was distinct from actin in amino acid composition and physicochemical properties. "DNase binding protein" was also is...

journal_title：Journal of biochemistry

authors： Kohama K,Holtzer H

更新日期：1981-02-01 00:00:00

abstract：:We have found that arachidonic (20 : 4) acid is indetectable in phosphatidylinositol and diacyglycerol extracted from Drosophila heads. After careful examinations of the lipid extraction processes and fatty acid detection system (gas-liquid chromatography), we excluded the possibility of the oxidation of polyunsaturat...

journal_title：Journal of biochemistry

authors： Yoshioka T,Inoue H,Kasama T,Seyama Y,Nakashima S,Nozawa Y,Hotta Y

更新日期：1985-09-01 00:00:00

abstract：:An H2O2-generating fraction was prepared from porcine thyroid homogenate by differential and Percoll-density gradient centrifugations. The fraction consisted of mainly fragmented plasma membranes as judged by marker enzyme analysis and electron microscopy. The fraction produced H2O2 by reaction with NADPH only in the ...

journal_title：Journal of biochemistry

authors： Nakamura Y,Ogihara S,Ohtaki S

更新日期：1987-11-01 00:00:00

abstract：:The receptor protein for the mitochondrial protein precursor synthesized in the cytosol was extensively purified from the mitochondrial membrane fraction by affinity column chromatography using a synthetic peptide containing the extrapeptide of ornithine aminotransferase as a ligand. The purified fraction contained tw...

journal_title：Journal of biochemistry

authors： Ono H,Tuboi S

更新日期：1990-06-01 00:00:00

abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

journal_title：Journal of biochemistry

authors： Kumagai JI,Ohta T,Imahori K

更新日期：1976-03-01 00:00:00

abstract：:Chicken brain enolase was found to show multiple forms (I, II and III) separable by DEAE-cellulose column chromatography, whereas enolase from chicken skeletal muscle showed a single form. Brain enolase I, enolase III and muscle enolase were purified to electrophoretic homogeneity. These three isozymes were dimeric en...

journal_title：Journal of biochemistry

authors： Tanaka M,Sugisaki K,Nakashima K

更新日期：1985-12-01 00:00:00

abstract：:Acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was purified from rat liver. The final preparation was judged to be nearly homogeneous from the results of sedimentation analysis. Ultrogel AcA-34 column chromatography, and phosphocellulose column chromatography. The molecular weight of th...

journal_title：Journal of biochemistry

authors： Osumi T,Hashimoto T,Ui N

更新日期：1980-06-01 00:00:00

abstract：:pKY2289, a ColE1::Tn3 derivative, useful for direct selection of cells carrying a hybrid plasmid, was deleted of its mobility functions and parts of the transposon including the left inverted repeat. This deletion mutant, named pKY2700 expresses low levels of colicin E1 synthesis even in recA cells. A nitrosoguanidine...

journal_title：Journal of biochemistry

authors： Ozaki LS,Kimura A,Shimada K,Takagi Y

更新日期：1982-04-01 00:00:00