Abstract:
:We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole, we isolated a 35-kDa Ca(2+)-dependent lectin (XCL-1) from adult Xenopus serum and cloned its cDNA. Although XCL-1 gene expression was not induced in the regenerating tails, we isolated a cDNA for an XCL-1-related protein (XCL-2) by reverse transcription-polymerase chain reaction. In contrast to the XCL-1 gene, XCL-2 gene expression was significantly increased in the regenerating tails, suggesting its role in tail regeneration. Although both XCL-1 and XCL-2 belong to a recently identified Xenopus lectin family (X-lectins), XCL-1 and XCL-2 exhibit distinct developmental gene expression from two other known X-lectin members, both of which are expressed principally in the embryonic stage, whereas the XCL-1 and XCL-2 genes are predominantly expressed in the adult and middle/late tadpole stages, respectively, suggesting multiple functions of X-lectin family members. Thus, the presence of multifunctional Ca(2+)-dependent lectin family and the induction of the member gene in regenerating organs are conserved among insects and vertebrates.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo Tdoi
10.1093/jb/mvm048subject
Has Abstractpub_date
2007-04-01 00:00:00pages
479-88issue
4eissn
0021-924Xissn
1756-2651pii
mvm048journal_volume
141pub_type
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021930
更新日期:1998-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131990
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journal_title:Journal of biochemistry
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更新日期:1998-08-01 00:00:00
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更新日期:1996-01-01 00:00:00
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更新日期:2007-11-01 00:00:00
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更新日期:1982-06-01 00:00:00
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更新日期:1984-08-01 00:00:00