Abstract：
:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH 6.5, the binding ratio of I-protein to myosin was 1:40 by molar ratio when the I-protein molecules highly specifically bound to myosin filaments. I-protein could maximally bind to myosin filaments at the molar ratio of 1:2.7. In this case, excess I-protein molecules remained in the supernatant after sedimentation, although the unbound I-protein could still bind to myosin filaments. Electron microscopic observations revealed that I-protein bundled myosin filaments in the low ionic strength solution (pH 6.5). Cage-like structures which were very similar to the Mg-paracrystals of non-muscle myosins were formed at pH 7.2. In gel filtration, the apparent molecular mass of I-protein was 100 kDa, while it was 50 kDa in SDS gel electrophoresis. Therefore, I-protein is regarded to be a homodimer of a 50 kDa subunit and can divalently bind to myosin molecules.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Ohashi K,Ishikawa K,Maruyama Kdoi
10.1093/oxfordjournals.jbchem.a122796subject
Has Abstractpub_date
1989-07-01 00:00:00pages
104-9issue
1eissn
0021-924Xissn
1756-2651journal_volume
106pub_type
杂志文章abstract：:The membrane protein syntaxin (originally named HPC-1) is involved in vesicle trafficking and required for neurotransmitter release at nerve terminals. The presence of syntaxin on target membranes is hypothesized to confer specificity to targeting and fusion via interactions with complementary vesicle-associated prote...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022337
更新日期：1999-04-01 00:00:00
abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134662
更新日期：1984-03-01 00:00:00
abstract：:In the preceding paper [Maita, T., Miyanishi, T., Matsuzono, K., Tanioka, Y., & Matsuda, G. (1991) J. Biochem. 110, 68-74], we reported the amino-terminal 837-residue sequence of the heavy chain of adult chicken pectoralis muscle myosin. This paper describes the carboxyl terminal 1,097-residue sequence and the linkage...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123546
更新日期：1991-07-01 00:00:00
abstract：:The entire mitochondrial genome of Rana catesbeiana was cloned into a plasmid vector pBR322 at the unique BamHI site and the nucleotide sequences of the ND2 gene and of its flanking genes were determined. The ND2 gene was encoded by 1,033 base pairs and, as deduced from the nucleotide sequence, the ND2 product consist...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122295
更新日期：1988-03-01 00:00:00
abstract：:The enthalpies of transfer of several amino acids and diglycine from water to aqueous polyols (glycerol, xylitol, sorbitol, and inositol) have been calorimetrically determined in order to clarify the mechanism of polyol-induced stabilization of proteins. The obtained enthalpy data were combined with the data on free e...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a133639
更新日期：1981-12-01 00:00:00
abstract：:The time course of binding of N-ethylmaleimide (NEM) to the SR was measured at pH 7.5 in the presence or absence of ATP or ADP. The following results were obtained. 1. Both in the presence and absence of nucleotide, the ATPase [EC 3.6.1.3] activity decreased linearly with increase in the amount of NEM bound to the fra...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a131109
更新日期：1976-03-01 00:00:00
abstract：:Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022645
更新日期：2000-04-01 00:00:00
abstract：:In a previous report (J. Biochem. 89, 1333-1335, 1981) we showed that 30 degrees C-treatment (pCa less than 6 and 2 mM MgCl2), like EDTA-treatment, caused a reversible removal of regulatory light-chains from Akazara adductor myosin. Utilizing the heat-treatment, we now show (a) that not half but the total removal of r...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134216
更新日期：1983-02-01 00:00:00
abstract：:We designed mutant lysozymes with N-glycosylation signal sequences (Asn48-Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkholder minimum medium, N-glycosylation occurred in both lysozymes....
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021201
更新日期：1996-01-01 00:00:00
abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123919
更新日期：1992-10-01 00:00:00
abstract：:Basigin belongs to the immunoglobulin superfamily and may be related to the primordial form of the superfamily. Human basigin cDNA was isolated and sequenced, and the predicted protein structure was compared with that of mouse basigin and two related molecules, embigin and the chicken blood-brain barrier antigen HT7. ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a123657
更新日期：1991-11-01 00:00:00
abstract：:The process of mitochondrial dysfunction in ischemic rat liver was studied. A close correlation was found between decrease in the mitochondrial adenine nucleotide content and deterioration of oxidative phosphorylation capacity. The level of total adenine nucleotides, which was 15--20 nmol/mg protein in mitochondria is...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134380
更新日期：1983-08-01 00:00:00
abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a134952
更新日期：1984-10-01 00:00:00
abstract：:Monoclonal antibody 5E5 recognized an intranuclear antigen of neurons in the rat. We isolated 5E5cDNA and determined its nucleotide and deduced amino acid sequences. The 5E5cDNA had an open reading frame of 825 amino acids and its amino acid sequence showed no significant homology to any protein or to any DNA binding ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124866
更新日期：1995-07-01 00:00:00
abstract：:Upon rehydration of lyophilized Escherichia coli cells with phosphate buffer containing [14C]isopentenyl pyrophosphate (IPP), 14C was incorporated into the cells. Radioactivity was found in ubiquinone-8, an unidentified precursor of ubiquinone-8, demethylmenaquinone-8 and phosphate esters of all-trans-octaprenol and c...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a135577
更新日期：1986-04-01 00:00:00
abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a125022
更新日期：1995-12-01 00:00:00
abstract：:A rat liver-specific antigen (RLSA) solubilized with the nonionic detergent non-anonyl-N-methylglucamide was purified through affinity column chromatography with a monoclonal antibody and by high-performance liquid chromatography with a hydroxylapatite column. The purified RLSA showed a single band on sodium dodecyl s...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a122134
更新日期：1987-10-01 00:00:00
abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132157
更新日期：1978-09-01 00:00:00
abstract：:The rates of synthesis and degradation of arginosuccinate synthetase in rat liver under various dietary conditions were determined. The relative rate of the enzyme synthesis in the livers of rats fed on 70% casein diet was 4.0 times greater than that for rats fed on 5% casein diet. The rate constants of degradation (K...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:A cell-wall-associated proteinase from Lactobacillus helveticus CP790, grown in skim milk, was purified to homogeneity by DEAE ion exchange chromatography in the presence of EDTA. Its molecular weight was estimated to be 45,000 by SDS-PAGE and also by the recovery of proteinase activity only from this fraction of SDS-...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a124247
更新日期：1993-11-01 00:00:00
abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvm045
更新日期：2007-03-01 00:00:00
abstract：:p40(phox) of the phagocyte NADPH oxidase forms a complex with p67(phox) in cytosol, and coincidentally decreases in patients who lack p67(phox). Here we investigated the mode of translocation of p40(phox) to the membrane, its cytoskeletal localization on activation of the NADPH oxidase, and the dependency of its expre...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a022815
更新日期：2000-11-01 00:00:00
abstract：:The TATA box-binding factor TFIID mediates transcriptional regulation through interactions with various regulatory factors and putative participation in reconfiguration of nucleosomes, utilizing its components, which include TATA box-binding protein (TBP) and TBP-associated factors (TAFs). Our and other previous studi...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a021626
更新日期：1997-03-01 00:00:00
abstract：:The DNA fragment containing the phoS gene, a regulatory gene for alkaline phosphatase, has been isolated from Escherichia coli K-12 chromosomal DNA by cutting off the DNA with Hind III restriction enzyme and by cloning the gene with plasmid vector pTP 4 which was constructed in this study. The isolated fragment was of...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a133972
更新日期：1982-09-01 00:00:00
abstract：:The lifetime of the purple intermediate formed from D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3] and a neutral D-amino acid under anaerobic conditions was measured with a series of neutral D-amino acids. The lifetime increases with increase in the number of carbons in the side chain...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132295
更新日期：1978-12-01 00:00:00
abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132254
更新日期：1978-12-01 00:00:00
abstract：:Previously, we purified the cathepsin B mRNA 3'-untranslated-region-binding protein (CBBP) from Sarcophaga and suggested its participation in the translational regulation of cathepsin B mRNA in this insect. In this study, we isolated a full length cDNA for CBBP. CBBP was an RNA-binding protein that contained four RGG ...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a002881
更新日期：2001-03-01 00:00:00
abstract：:The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/oxfordjournals.jbchem.a132812
更新日期：1980-03-01 00:00:00
abstract：:The modification of proteins by SUMO (small ubiquitin-like modifier) regulates various cellular processes. Sumoylation often occurs on a specific lysine residue within the consensus motif psiKxE/D. However, little is known about the specificity and selectivity of SUMO target sites. We describe here a SUMO assay with p...
journal_title：Journal of biochemistry
pub_type： 杂志文章
doi：10.1093/jb/mvn039
更新日期：2008-07-01 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...
journal_title：Journal of biochemistry
pub_type： 杂志文章,评审
doi：10.1093/jb/mvy063
更新日期：2018-10-01 00:00:00
