Isolation and purification of a rat liver-specific antigen from hepatocyte membrane.

abstract：:Human, bovine, and mouse C1q, a subcomponent of the first complement component, were purified, and both globular (GF) and collagen-like fragments (CLF) were isolated from human and bovine C1q. Antisera were produced in rabbits with these C1q or fragments, and F(ab')2 of immunoglobulin G (IgG) was purified from the ant...

journal_title：Journal of biochemistry

authors： Yonemasu K,Sasaki T,Shimizu A

更新日期：1983-04-01 00:00:00

abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...

journal_title：Journal of biochemistry

authors： Poetsch A,Berzborn RJ,Heberle J,Link TA,Dencher NA,Seelert H

更新日期：2007-03-01 00:00:00

abstract：:d-Aspartate is found in the nervous and reproductive system and participates in various physiological roles. While several lines of evidence suggest that this amino acid has an endogenous origin, the enzyme responsible for mammalian d-Asp biosynthesis has not yet been identified. We show that mammalian serine racemase...

journal_title：Journal of biochemistry

authors： Ito T,Hayashida M,Kobayashi S,Muto N,Hayashi A,Yoshimura T,Mori H

更新日期：2016-12-01 00:00:00

abstract：:Lipoamide dehydrogenase (LADase) was purified to homogeneity from rat liver mitochondria, and the intracellular distribution and biosynthesis of the LADase were investigated with antibody prepared against the purified enzyme. 1) LADase activity was mostly found in mitochondria; the activity in cytosol was about one-te...

journal_title：Journal of biochemistry

authors： Matuda S,Saheki T

更新日期：1982-02-01 00:00:00

abstract：:Phosphoenolpyruvate (PEP) carboxylase [EC 4.1.1.31] of E. coli was inactivated by 2,4,6-trinitrobenzene sulfonate (TNBS), a reagent known to attack amino groups in polypeptides. When the modified enzyme was hydrolyzed with acid, epsilon-trinitrophenyl lysine (TNP-lysine) was identified as a product. Close similarity o...

journal_title：Journal of biochemistry

authors： Naide A,Izui K,Yoshinaga T,Katsuki H

更新日期：1979-02-01 00:00:00

abstract：:Accumulation of pyrophosphate induced by acetate administration was investigated in rat liver in situ and in perfused rat liver. Intraperitoneal injection of acetate into rats increased the pyrophosphate concentration in the liver to about 2 mumol/g liver, which was 200 times that in control liver. Perfusion of liver ...

journal_title：Journal of biochemistry

authors： Yamada T,Inoue T,Nishida T,Furuya E,Tagawa K

更新日期：1988-11-01 00:00:00

abstract：:We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2b, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(gamma-maleimidobutyryloxy...

journal_title：Journal of biochemistry

authors： Fujiwara K,Kaminishi Y,Kitagawa T,Tsuru D,Yabuuchi M,Kanetake H,Nomata K

更新日期：1998-07-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00

abstract：:Raft is a mobile membrane subdomain enriched in sphingolipid and cholesterol and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), migh...

journal_title：Journal of biochemistry

authors： Maekawa S,Morii H,Kumanogoh H,Sano M,Naruse Y,Sokawa Y,Mori N

更新日期：2001-05-01 00:00:00

abstract：:The role of mannan chains in the formation and secretion of active acid phosphatase of yeast (Saccharomyces cerevisiae), a repressible cell surface mannoprotein, was studied in yeast protoplast systems by using tunicamycin at various temperatures. At 30 degrees C, tunicamycin-treated protoplasts did not produce active...

journal_title：Journal of biochemistry

authors： Mizunaga T,Izawa M,Ikeda K,Maruyama Y

更新日期：1988-02-01 00:00:00

abstract：:ZFP36L1 is an RNA-binding protein responsible for mRNA decay in the cytoplasm. ZFP36L1 has also been suggested as a nuclear-cytoplasmic shuttling protein because it contains a potential nuclear localization signal and a nuclear export signal. However, it remains unclear how the nuclear localization of ZFP36L1 is contr...

journal_title：Journal of biochemistry

authors： Matsuura Y,Noguchi A,Sakai S,Yokota N,Kawahara H

更新日期：2020-11-01 00:00:00

abstract：:Effects of rice bran agglutinin (RBA) on human monoblastic leukemia U937 cells were examined in comparison with those of wheat germ agglutinin (WGA) and Viscum album agglutinin (VAA). These lectins inhibit cell growth, and several lines of evidence indicate that the growth inhibition is caused by the induction of apop...

journal_title：Journal of biochemistry

authors： Miyoshi N,Koyama Y,Katsuno Y,Hayakawa S,Mita T,Ohta T,Kaji K,Isemura M

更新日期：2001-12-01 00:00:00

abstract：:It has been reported that two kinds of Cd-binding peptide (Cd-BP1 and Cd-BP2) are induced in fission yeast upon exposure to Cd, and that they consist of the same unit peptide (cadystin), but Cd-BP1 binds 1.5 times more Cd atoms per cadystin than Cd-BP2 (Murasugi, A., Wada, C., & Hayashi, Y. (1981) J. Biochem. 90, 1561...

journal_title：Journal of biochemistry

authors： Murasugi A,Wada Nakagawa C,Hayashi Y

更新日期：1984-11-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:Bacillus licheniformis alpha-amylase (BLA) is widely used in various procedures of starch degradation in the food industry, and a BLA species with improved activity at higher temperature and under acidic conditions is desirable. Two BLA species, designated as PA and MA, have been isolated from the wild-type B. licheni...

journal_title：Journal of biochemistry

authors： Lee S,Oneda H,Minoda M,Tanaka A,Inouye K

更新日期：2006-06-01 00:00:00

abstract：:We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion fragments of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universa...

journal_title：Journal of biochemistry

authors： Kang DC,Takeshige K,Minakami S

更新日期：1990-12-01 00:00:00

abstract：:Reports vary on the role of growth hormone (GH) in adipocyte differentiation. In this study, we showed that GH exerted dual effects depending on the stage of differentiation, using a serum-free culture of 3T3-L1 preadipocytes. GH promoted the differentiation when added to the medium during differentiation-inducing tre...

journal_title：Journal of biochemistry

authors： Tominaga S,Morikawa M,Osumi T

更新日期：2002-12-01 00:00:00

abstract：:Both beef heart cytochrome oxidase and bacteriorhodopsin of Halobacterium halobium were reconstituted into liposomes by the sonication-cholate dialysis method. The proteoliposomes showed the respiratory control ratio of 4.2, and steady-state illumination of the vesicles lead to the 2.7-fold stimulation of the oxidase ...

journal_title：Journal of biochemistry

authors： Miki T,Orii Y,Mukohata Y

更新日期：1987-07-01 00:00:00

abstract：:The fluorescence characteristics of product (I), formed during the lipid peroxidation of rat liver phosphatidylcholine liposomes containing glycine, and fluorescent product (II), derived from the reaction of malonaldehyde with glycine, were examined to elucidate the mechanism of fluorescent chromophore formation. Fluo...

journal_title：Journal of biochemistry

authors： Shimasaki H,Hirai N,Ueta N

更新日期：1988-11-01 00:00:00

abstract：:The three-dimensional structure of rat-liver acyl-CoA oxidase-II (ACO-II) in a complex with a C12-fatty acid was solved by the molecular replacement method based on the uncomplexed ACO-II structure. The crystalline form of the complex was obtained by cocrystallization of ACO-II with dodecanoyl-CoA. The crystalline com...

journal_title：Journal of biochemistry

authors： Tokuoka K,Nakajima Y,Hirotsu K,Miyahara I,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Tojo H,Miura R

更新日期：2006-04-01 00:00:00

abstract：:A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

journal_title：Journal of biochemistry

authors： Oda S,Sato M,Toyoshima S,Osawa T

更新日期：1989-06-01 00:00:00

abstract：:The DNA-dependent protein kinase (DNA-PK) holoenzyme consists of a 470-kDa catalytic subunit (DNA-PKcs), a DNA-binding regulatory component known as Ku protein, and double-stranded DNA (dsDNA) with ends. We previously reported that the activity of DNA-PK in vitro is stimulated by non-histone chromosomal high mobility ...

journal_title：Journal of biochemistry

authors： Yumoto Y,Shirakawa H,Yoshida M,Suwa A,Watanabe F,Teraoka H

更新日期：1998-09-01 00:00:00

abstract：:Treatment of Sendai virus with p-azidophenyl-6-chloro-6-deoxy-beta-D-glucopyranoside (APG) caused chemical modification of the viral envelope under UV irradiation, which did not affect the hemagglutinin activity of the virus but inhibited the hemolytic activity. Also, the transfer of phospholipid from the viral envelo...

journal_title：Journal of biochemistry

authors： Jizomoto H,Arita H,Sugita K,Kawanami J,Sato K,Kuriyama K

更新日期：1980-10-01 00:00:00

abstract：:The circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of horse heart metmyoglobin and the following derivatives were measured in the Soret and near ultraviolet regions: metmyoglobin and its peroxide compound, and hydroxide, cyanide, azide, and fluoride derivatives. The heme-related CD bands in the ...

journal_title：Journal of biochemistry

authors： Kajiyoshi M,Anan FK

更新日期：1975-11-01 00:00:00

abstract：:A continuous cell-free protein synthesis system [Spirin et al. (1988) Science 242, 1162-1164] was modified so as to be suitable for coupled transcription-translation, a process useful for obtaining products of cloned genes or cDNAs. A reaction chamber equipped with an ultrafiltration membrane was newly designed and an...

journal_title：Journal of biochemistry

authors： Kigawa T,Yokoyama S

更新日期：1991-08-01 00:00:00

abstract：:The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and ...

journal_title：Journal of biochemistry

authors： Mizushima F,Minoura K,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2007-07-01 00:00:00

abstract：:The effects of polyamines on the breakdown of synthetic polynucleotides [poly(A), poly(C), and poly(U)] by E. coli ribonuclease I [ribonucleate 3'-oligonucleotidohydrolase, EC 3.1.4.23] and ribonuclease II [EC 3.1.4.1] have been studied. The degradation of poly(C) by RNase II was stimulated by spermine and spermidine,...

journal_title：Journal of biochemistry

authors： Kumagai H,Igarashi K,Yoshikawa M,Hirose S

更新日期：1977-02-01 00:00:00

abstract：:Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...

journal_title：Journal of biochemistry

authors： Furuichi H,Ozaki Y,Niki K,Akutsu H

更新日期：1990-11-01 00:00:00

abstract：:Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a medium containing 2,4-dichlorophenoxy-acetic acid (2,4-D) to one lacking it. A molecular weight of 80,000-85,000 for th...

journal_title：Journal of biochemistry

authors： Ozeki Y,Sakano K,Komamine A,Tanaka Y,Noguchi H,Sankawa U,Suzuki T

更新日期：1985-07-01 00:00:00