Effects of anion transport inhibitors on hemolysis of human erythrocytes under hydrostatic pressure.


:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hemolysis. In contrast, the degree of hypotonic hemolysis increased upon treatment with anion transport, inhibitors. From the relationship between the hemolysis at 200 mPa and anion transport, it was found that high-pressure-induced hemolysis was suppressed by the covalent binding of anion transport inhibitors to band 3. This idea was supported by the finding that the hemolysis at 200 mPa of trypsin-treated erythrocytes was suppressed by DIDS. Furthermore, the spectrin content in vesicles which are released from erythrocyte ghost by dimyristoyl-phosphatidylcholine decreased upon DIDS labeling of band 3, but did not change upon PLP labeling. These results suggest that the interaction of the cytoplasmic domain of band 3 with spectrin, perhaps via ankyrin, is tightened by the covalent binding of bulky ligands to the exofacial domain of band 3.


J Biochem


Journal of biochemistry


Yamaguchi T,Matsumoto M,Kimoto E




Has Abstract


1995-10-01 00:00:00












  • Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte lysate.

    abstract::During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwamuro S,Saeki M,Kato S

    更新日期:1999-07-01 00:00:00

  • A Raman study on the C(4)=O stretching mode of flavins in flavoenzymes: hydrogen bonding at the C(4)=O moiety.

    abstract::Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

    更新日期:1997-06-01 00:00:00

  • Novel actin depolymerizing macrolide aplyronine A.

    abstract::Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actin. The kinetics of depolymerization suggest that aplyronine A...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saito S,Watabe S,Ozaki H,Kigoshi H,Yamada K,Fusetani N,Karaki H

    更新日期:1996-09-01 00:00:00

  • pH- and salt-dependent self-assembly of human Rad51 protein analyzed as fluorescence resonance energy transfer between labeled proteins.

    abstract::Human HsRad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. We have characterized the self-assembly of HsRad51 by measuring the fluorescence resonance energy transfer from the fluorescein-labeled protein to the rhodamine-labeled protein. Self-as...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yoshioka K,Yumoto-Yoshioka Y,Fleury F,Takahashi M

    更新日期:2003-05-01 00:00:00

  • Inositol 1,4,5-trisphosphate induced calcium release from corn coleoptile microsomes.

    abstract::The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release from microsomes of corn coleoptiles was investigated. Addition of micromolar concentrations of IP3 to Ca2+ loaded microsomes resulted in rapid release of 20-30% of sequestered Ca2+. Maximal and half maximal Ca2+ release occurred at 20 and 8 microM of IP3...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Reddy AS,Poovaiah BW

    更新日期:1987-03-01 00:00:00

  • ATPase activities of the two cross-linked species of actin and myosin.

    abstract::We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamamoto K

    更新日期:1993-12-01 00:00:00

  • N-terminal amino acid sequence of human urinary prokallikrein.

    abstract::The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

    更新日期:1986-03-01 00:00:00

  • Structural basis for induced-fit binding of Rho-kinase to the inhibitor Y-27632.

    abstract::Rho-kinase is a main player in the regulation of cytoskeletal events and a promising drug target in the treatment of both vascular and neurological disorders. Here we report the crystal structure of the Rho-kinase catalytic domain in complex with the specific inhibitor Y-27632. Comparison with the structure of PKA bou...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamaguchi H,Miwa Y,Kasa M,Kitano K,Amano M,Kaibuchi K,Hakoshima T

    更新日期:2006-09-01 00:00:00

  • Synthesis of the acid-soluble proteins in early cleaving embryos of the sea urchin. Cyclic synthesis of histones.

    abstract::Synthesis of the acid-soluble proteins in the early cleavage stage of the sea urichin, Hemicentrotus pulcherrimus, was investigated. As detected by the incorporation of lysine, the acid-soluble proteins were synthesized periodically even before the first cleavage, differing from the pattern of incorporation of tryptop...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kano K,Mano Y

    更新日期:1976-09-01 00:00:00

  • Overexpression and purification of the recombinant Ca2+-binding protein, apoaequorin.

    abstract::The small, monomeric Ca2+-binding photoprotein, aequorin, emits blue light by an intramolecular reaction when mixed with Ca2+. The photoprotein is made up of coelenterazine and molecular oxygen, bound noncovalently to apoaequorin (apoprotein). The chemical steps leading to light emission, involving the oxidative degra...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inouye S,Aoyama S,Miyata T,Tsuji FI,Sakaki Y

    更新日期:1989-03-01 00:00:00

  • Establishment and characterization of a novel human rheumatoid fibroblast-like synoviocyte line, MH7A, immortalized with SV40 T antigen.

    abstract::A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Miyazawa K,Mori A,Okudaira H

    更新日期:1998-12-01 00:00:00

  • Lecithin: cholesterol acyltransferase is insufficient to prevent oxidative modification of low-density lipoprotein.

    abstract::We tried to confirm the antioxidative capability of lecithin:cholesterol acyltransferase (LCAT) reported by Vohl et al. [Biochemistry (1999) 38, 5976-5981]. The enzyme solution protected LDL against oxidation. However, this protection was not due to LCAT enzyme, but to some unknown low-molecular-weight substance(s) in...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Murakami Y,Kamiyama S,Howlader ZH,Yamato T,Komai M,Furukawa Y

    更新日期:2002-01-01 00:00:00

  • Nucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver.

    abstract::Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Komiya H,Takemura S

    更新日期:1979-10-01 00:00:00

  • Two J domains ensure high cochaperone activity of DnaJ, Escherichia coli heat shock protein 40.

    abstract::Heat shock protein 70 (Hsp70) chaperone systems consist of Hsp70, Hsp40 and a nucleotide-exchange factor and function to help unfolded proteins achieve their native conformations. Typical Hsp40s assume a homodimeric structure and have both chaperone and cochaperone activity. The dimeric structure is critical for chape...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Uchida T,Kanemori M

    更新日期:2018-08-01 00:00:00

  • The methylamine oxidizing system of Pseudomonas AM1 reconstituted with purified components.

    abstract::The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fukumori Y,Yamanaka T

    更新日期:1987-02-01 00:00:00

  • A positive regulatory sequence of the Saccharomyces cerevisiae ENO1 gene.

    abstract::ENO1-'lacZ fusions with various lengths of the ENO1 5'-flanking region were constructed on various types of yeast plasmid vectors. The fully expressed level of beta Gal directed by ENO1-'lacZ fusions differed depending on the type of vector, but on any type of vector, beta Gal activity was not greatly influenced by th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Uemura H,Shiba T,Machida M,Matsui I,Jigami Y,Tanaka H

    更新日期:1987-07-01 00:00:00

  • An isoform of microtubule-associated protein 4 inhibits kinesin-driven microtubule gliding.

    abstract::Recently, we revealed that microtubule-associated protein (MAP) 4 isoforms, which differ in the number of repeat sequences, alter the microtubule surface properties, and we proposed a hypothesis stating that the change in the surface properties may regulate the movements of microtubule motors [Tokuraku et al. (2003) J...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tokuraku K,Noguchi TQ,Nishie M,Matsushima K,Kotani S

    更新日期:2007-04-01 00:00:00

  • Incorporation of a ganglioside and spin-labeled ganglioside analogue into cell and liposomal membranes.

    abstract::When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

    更新日期:1982-06-01 00:00:00

  • Actin-binding specificity of marine macrolide toxins, mycalolide B and kabiramide D.

    abstract::An actin-depolymerizing marine natural product, mycalolide B, and a related compound, kabiramide D, were labeled with biocytin, a biotin derivative, and used to specify target molecules in cultured rat 3Y1 fibroblasts. Mycalolide B exhibited the ability to bind to various intracellular proteins, probably through the M...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Wada S,Matsunaga S,Saito S,Fusetani N,Watabe S

    更新日期:1998-05-01 00:00:00

  • Near-infrared absorption spectra of light harvesting bacteriochlorophyll protein complexes from Chromatium vinosum.

    abstract::Light harvesting (LH) bacteriochlorophyll (Bchl) protein complexes were isolated from chromatophores of Chromatium vinosum, by the combination of detergent solubilization, sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and hydroxyapatite chromatography. Addition of the absorption spectra of thes...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hayashi H,Morita S

    更新日期:1980-11-01 00:00:00

  • Effects of drugs, salts, and phospholipid vesicles on hemoglobin release from hydrostatic pressure-treated human erythrocytes.

    abstract::When human erythrocytes subjected to a pressure of 2.0 kbar were incubated at 0 degree C and atmospheric pressure, hemoglobin was released from the membrane. Effects of drugs, salts, and phospholipid vesicles on hemoglobin release were examined. The hemoglobin release was suppressed by the addition of amphiphathic dru...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamaguchi T,Kuranoshita K,Harano T,Kimoto E

    更新日期:1993-04-01 00:00:00

  • Gene organization of pldA and pldB, the structural genes for detergent-resistant phospholipase A and lysophospholipase L2 of Escherichia coli.

    abstract::The genes coding for the phospholipid degradation enzymes in E. coli, detergent-resistant (DR-) phospholipase A (pldA) and lysophospholipase L2 (pldB), were cloned together on the plasmid pKO1 (Homma, H., Kobayashi, T., Ito, Y., Kudo, I., Inoue, K., Ikeda, H., Sekiguchi, M., & Nojima, S. (1983) J. Biochem. 94, 2079-20...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kobayashi T,Kudo I,Homma H,Karasawa K,Inoue K,Ikeda H,Nojima S

    更新日期:1985-10-01 00:00:00

  • Significance of Thr182 in the nucleotide-exchange and GTP-hydrolysis reactions of the alpha subunit of GTP-binding protein Gi2.

    abstract::The crystal structures of the GTP- and GDP-bound alpha subunits of heterotrimeric GTP-binding proteins were recently determined, and a conserved Thr residue in the G2 (linker 2) region of the alpha subunits, which corresponds to Thr182 in Gi2 alpha, was deduced to interact with the gamma-phosphate of GTP and Mg2+. To ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishina H,Nimota K,Kukimoto I,Maehama T,Takahashi K,Hoshino S,Kanaho Y,Katada T

    更新日期:1995-11-01 00:00:00

  • Structure of polygalactosamine produced by Aspergillus parasiticus.

    abstract::The structure of polygalactosamine purified from the culture fluid of Aspergillus parasiticus AHU 7165 was studied. Partial acid hydrolysis of this polysaccharide, in which 55 to 65% of the monosaccharide residues were N-unsubstituted, gave a series of galactosamine oligosaccharides (dimer to hexamer) in a good yield....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takada H,Araki Y,Ito E

    更新日期:1981-04-01 00:00:00

  • Myoclonus, motor deficits, alterations in emotional responses and monoamine metabolism in epsilon-sarcoglycan deficient mice.

    abstract::Mutations of epsilon-sarcoglycan gene (SGCE) have been implicated in myoclonus-dystonia (M-D), a movement disorder. To determine the pathophysiology of M-D, we produced Sgce knockout mice and found that the knockout mice exhibited myoclonus, motor impairments, hyperactivity, anxiety, depression, significantly higher l...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yokoi F,Dang MT,Li J,Li Y

    更新日期:2006-07-01 00:00:00

  • Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

    abstract::E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

    更新日期:1983-12-01 00:00:00

  • Purification and characterization of a glucoamylase from Aspergillus saitoi.

    abstract::1. A major glucoamylase [EC] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takahashi T,Inokuchi N,Irie M

    更新日期:1981-01-01 00:00:00

  • Heat-induced aggregation of recombinant erythropoietin in the intact and deglycosylated states as monitored by gel permeation chromatography combined with a low-angle laser light scattering technique.

    abstract::Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

    更新日期:1992-11-01 00:00:00

  • Conformational changes in Mycobacterium smegmatis glutamine synthetase induced by certain divalent cations.

    abstract::Manganese ion, like Mg2+, has been found to produce high biosynthetic activity of the unadenylylated form of glutamine synthetase obtained from Mycobacterium smegmatis, and the activity with each of these cations was decreased by the adenylylation of the enzyme. Further, the gamma-glutamyltransferase reaction was cata...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsuoka K,Kimura K

    更新日期:1985-04-01 00:00:00

  • Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.

    abstract::Haematopoiesis and blood vessel formation are closely associated, with several molecules employed by both systems. Recently, vasohibin-1 (VASH1), an endothelium-derived negative feedback regulator of angiogenesis, has been isolated and characterized. VASH1 is induced by VEGF or bFGF in endothelial cells (ECs) and inhi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Naito H,Kidoya H,Sato Y,Takakura N

    更新日期:2009-05-01 00:00:00