Effects of anion transport inhibitors on hemolysis of human erythrocytes under hydrostatic pressure.


:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hemolysis. In contrast, the degree of hypotonic hemolysis increased upon treatment with anion transport, inhibitors. From the relationship between the hemolysis at 200 mPa and anion transport, it was found that high-pressure-induced hemolysis was suppressed by the covalent binding of anion transport inhibitors to band 3. This idea was supported by the finding that the hemolysis at 200 mPa of trypsin-treated erythrocytes was suppressed by DIDS. Furthermore, the spectrin content in vesicles which are released from erythrocyte ghost by dimyristoyl-phosphatidylcholine decreased upon DIDS labeling of band 3, but did not change upon PLP labeling. These results suggest that the interaction of the cytoplasmic domain of band 3 with spectrin, perhaps via ankyrin, is tightened by the covalent binding of bulky ligands to the exofacial domain of band 3.


J Biochem


Journal of biochemistry


Yamaguchi T,Matsumoto M,Kimoto E




Has Abstract


1995-10-01 00:00:00












  • Engineering of artificial cell-adhesive proteins by grafting EILDVPST sequence derived from fibronectin.

    abstract::Fibronectin contains at least two distinct oligopeptide sequences serving as signals for the interaction with cell surface adhesion receptors termed integrins. One of these sequences, Arg-Gly-Asp-Ser (RGDS) tetrapeptide, was shown to be transferred to a truncated form of Staphylococcal IgG-binding protein (hereafter r...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Maeda T,Oyama R,Titani K,Sekiguchi K

    更新日期:1993-01-01 00:00:00

  • Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase.

    abstract::Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

    更新日期:2003-12-01 00:00:00

  • Repression of GR-mediated expression of the tryptophan oxygenase gene by the SWI/SNF complex during liver development.

    abstract::The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG1 in the expression...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inayoshi Y,Kaneoka H,Machida Y,Terajima M,Dohda T,Miyake K,Iijima S

    更新日期:2005-10-01 00:00:00

  • On the denaturation of porcine erythrocyte catalase with alkali, urea, and guanidine hydrochloride in relation to its subunit structure.

    abstract::The dissociation of porcine erythrocyte catalase [EC] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takeda A,Hirano K,Shiroya Y,Samejima T

    更新日期:1983-04-01 00:00:00

  • Hepatic uptake of bilirubin diglucuronide: analysis by using sinusoidal plasma membrane vesicles.

    abstract::In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IM

    更新日期:1990-05-01 00:00:00

  • Effects of drugs, salts, and phospholipid vesicles on hemoglobin release from hydrostatic pressure-treated human erythrocytes.

    abstract::When human erythrocytes subjected to a pressure of 2.0 kbar were incubated at 0 degree C and atmospheric pressure, hemoglobin was released from the membrane. Effects of drugs, salts, and phospholipid vesicles on hemoglobin release were examined. The hemoglobin release was suppressed by the addition of amphiphathic dru...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamaguchi T,Kuranoshita K,Harano T,Kimoto E

    更新日期:1993-04-01 00:00:00

  • Protein S is essential for the activated protein C-catalyzed inactivation of platelet-associated factor Va.

    abstract::The prothrombin-converting activity of Factor Xa was enhanced by thrombin-stimulated Factor V-deficient platelets and supplementary extraneous Factor Va, and also by thrombin-stimulated normal human platelets. Both extraneous Factor Va and intra-platelet Factor Va were equally inactivated by a gamma-carboxyglutamic ac...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suzuki K,Nishioka J,Matsuda M,Murayama H,Hashimoto S

    更新日期:1984-08-01 00:00:00

  • Regio- and stereoselectivity in propranolol metabolism by dog liver microsomes and the expressed dog CYP2D15.

    abstract::We have studied the regio- and stereoselectivity of ring-hydroxylation and N-desisopropylation of S(-)- and R(+)-propranolol, using dog liver microsomes and the expressed dog CYP2D15 in insect cells. In dog liver microsomes, 4-hydroxylation was the preferred pathway in S(-)-propranolol oxidation, while N-desisopropyla...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tasaki T,Iwata H,Kazusaka A,Fujita S

    更新日期:1998-04-01 00:00:00

  • A method to determine the affinity of heparin to thrombin and antithrombin III on equilibrium gel permeation chromatography.

    abstract::Equilibrium gel permeation chromatography was employed to determine the ability of heparin to form complexes with thrombin and antithrombin III. In the eluate from a Sephacryl S-200 column, heparin caused a peak and then a trough in the fluorescence of 48 nM antithrombin III or 63 nM thrombin. The peak-heights with kn...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oshima G,Uchiyama H,Nagasawa K

    更新日期:1984-10-01 00:00:00

  • Changes in myosin isozymes during development of chicken breast muscle.

    abstract::The patterns of myosin isozymes in embryonic and adult chicken pectoralis muscle were examined by electrophoresis in a non-denaturing gel system (pyrophosphate acrylamide gel electrophoresis), and both light chains and heavy chains of embryonic and adult myosin isozymes were compared. In pyrophosphate acrylamide gel e...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takano-Ohmuro H,Obinata T,Masaki T,Mikawa T

    更新日期:1982-04-01 00:00:00

  • Static and kinetic studies by fluorometry on the interaction between gluconolactone and glucoamylase from Rh. niveus.

    abstract::A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohnishi M,Yamashita T,Hiromi K

    更新日期:1977-01-01 00:00:00

  • Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae.

    abstract::Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mima J,Suzuki H,Takahashi M,Hayashi R

    更新日期:2002-12-01 00:00:00

  • Purificantion and characterization of inorganic pyrophosphatase from Thiobacillus thiooxidans.

    abstract::An inorganic pyrophosphatase [EC] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum acti...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tominaga N,Mori T

    更新日期:1977-02-01 00:00:00

  • Correlation between the catalase level in tumor cells and their sensitivity to N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD).

    abstract::N-beta-Alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD) exhibits selective cytotoxicity toward certain human tumor cell lines. 5-S-GAD has been shown to release hydrogen peroxide autonomously. Hydrogen peroxide is converted to water and oxygen by catalase. The purpose of this study is to determine whether ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishikawa T,Nishikawa S,Akiyama N,Natori S

    更新日期:2004-04-01 00:00:00

  • Midkine (MK) expression in extraembryonic tissues, amniotic fluid, and cerebrospinal fluid during mouse embryogenesis.

    abstract::Midkine (MK) and pleiotrophin (PTN) constitute a new family of heparin-binding growth factors. Extraembryonic membranes and the placenta of the mouse expressed MK mRNA at 11.5 days gestation. While the MK mRNA level in extraembryonic membranes decreased during embryogenesis, that in the placenta remained unchanged. Im...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Obama H,Tsutsui J,Ozawa M,Yoshida H,Yoshida Y,Osame M,Muramatsu T

    更新日期:1995-07-01 00:00:00

  • Transformation of leukotriene D4 catalyzed by lysosomal cathepsin H of rat liver.

    abstract::Among several intracellular protease tested, cathepsin H transformed leukotriene D4 to E4 with a release of glycine in a stoichiometric quantity. Under the optimal conditions the rate of leukotriene D4 transformation by cathepsin H was about 3% of the hydrolysis rate of alpha-N-benzoyl-DL-arginine-2-naphthylamide whic...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yokota K,Shono F,Yamamoto S,Kominami E,Katunuma N

    更新日期:1983-10-01 00:00:00

  • A spin-label and hydrogen-deuterium exchange reaction kinetics study of protein-lipid interactions in lipid-replaced Ca2+-ATPase of rabbit skeletal muscle sarcoplasmic reticulum.

    abstract::The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Higashi K,Kirino Y

    更新日期:1983-12-01 00:00:00

  • Multiple forms of ribonuclease H from rat liver cytosol.

    abstract::Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sawai Y,Yanokura M,Tsukada K

    更新日期:1979-09-01 00:00:00

  • The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins.

    abstract::The plasma membrane, the outermost surface of eukaryotic cells, contains various substructures, such as protrusions or invaginations, which are associated with diverse functions, including endocytosis and cell migration. These structures of the plasma membrane can be considered as tubules or inverted tubules (protrusi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suetsugu S

    更新日期:2010-07-01 00:00:00

  • Crystallization and preliminary X-ray crystallographic studies of Thermus thermophilus HB8 MutM protein involved in repairs of oxidative DNA damage.

    abstract::MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

    更新日期:2000-01-01 00:00:00

  • Incorporation of glutamic acid into protein by a soluble system.

    abstract::A heat-labile, non-dialyzable factor(s) in soluble fractions from Escherichia coli strains and Bacillus subtilis was found to incorporate the radioactivity of [14C]glutamic acid into 95 degrees C CCl3COOH-insoluble fraction. Incorporation catalyzed by a partially purified factor from E. coli B required ATP, Mg2+, tRNA...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hashizume S,Honjoh T,Shoji M

    更新日期:1983-04-01 00:00:00

  • Effects of freezing on purified nitrite reductase from a denitrifier, Alcaligenes sp. NCIB 11015.

    abstract::The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

    更新日期:1985-11-01 00:00:00

  • Purification of an active gelatinase from collagen fiber fraction of granulation tissue in rats.

    abstract::Gelatinase was extracted at 60 degrees C from the collagen fiber-rich fraction of granulation tissue induced by carrageenin in rats. A large part of the extracted gelatinase was unbound to Zn-chelating Sepharose. The unbound gelatinase gave a single band corresponding to a molecular mass of 57 kDa on SDS-substrate PAG...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakagawa H,Hirata M,Hoshino K,Sakata K,Hatakeyama S

    更新日期:1990-09-01 00:00:00

  • Ca2+-induced distance change between points on actin and troponin in skeletal muscle thin filaments estimated by fluorescence energy transfer spectroscopy.

    abstract::Fluorescence resonance energy transfer spectroscopy has been used to study the spatial relationships between probes attached to actin and troponin in the reconstituted skeletal muscle thin filament in the presence and absence of Ca2+ ions. Gln-41 and the nucleotide-binding site of actin were selectively labeled with t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Miki M,Kobayashi T,Kimura H,Hagiwara A,Hai H,Maéda Y

    更新日期:1998-02-01 00:00:00

  • Disulfide bond interchange in Escherichia coli-derived recombinant human interferon-beta 1 under denaturing conditions.

    abstract::Disulfide bond interchange has been pointed out as a considerable problem in preparing recombinant proteins from Escherichia coli cells. This has been reported in the system of reducing denaturation followed by a refolding process, where incorrectly folded molecules are sometimes produced. As the possibility of disulf...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kimura S,Utsumi J,Yamazaki S,Shimizu H

    更新日期:1988-07-01 00:00:00

  • Soluble proteins from fowl feather keratin. I. Fractionation and properties.

    abstract::A method is described for the fractionation of reduced and alkylated proteins of fowl feather. Fowl feather extracts were chromatographed on a Sephadex G-75 column in 4 M urea containing 1 M NaCl and separated into four fractions, GF-1, 2, 3, and 4. The elution patterns were used to compare the components of different...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Akahane K,Murozono S,Murayama K

    更新日期:1977-01-01 00:00:00

  • Silent information regulator type-1 mediates amelioration of inflammatory response and oxidative stress in lipopolysaccharide-induced acute respiratory distress syndrome.

    abstract::Silent information regulator type-1 (SIRT1) is crucial during the development of acute respiratory distress syndrome (ARDS). We aimed to explore whether SIRT1 activation could protect against ARDS. SIRT1 was activated by its agonist SRT1720. ARDS was induced by intraperitoneal injection of 5 mg/kg lipopolysaccharide (...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhan Y,Yang C,Zhang Q,Yao L

    更新日期:2021-01-22 00:00:00

  • The Yin and Yang of tumor-derived extracellular vesicles in tumor immunity.

    abstract::Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamauchi T,Moroishi T

    更新日期:2020-11-23 00:00:00

  • Cloning of dihydrofolate reductase gene of Escherichia coli K12.

    abstract::Resistant strains for trimethoprim, a potent inhibitor of dihydrofolate reductase, were obtained by transforming the ligated products of Escherichia coli K12 DNA and plasmid pBR322 BamH I fragments. The strains carry a 13.6 kbp plasmid, pTP1, which contains the trimethoprim- and ampicillin-resistance determinant genes...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwakura M,Shimura Y,Tsuda K

    更新日期:1982-04-01 00:00:00

  • Developmental profiles of glycolipids in mouse small intestine.

    abstract::The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sato E,Uezato T,Fujita M,Nishimura K

    更新日期:1982-06-01 00:00:00