The localization of 4-O-methylglucuronic acid-containing glycosphingolipid on the cell surface of bivalve spermatozoa by use of an immunological method.

Abstract:

:Specific and high titer antisera against 4-O-methylglucuronic acid-containing glycosphingolipid (Lipid IV) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits. The antisera were found to agglutinate spermatozoa of three fresh-water bivalves, H. schlegelii, Anodonta woodiana, and Cristaria plicata (Palaeoheterodonta), but they did not agglutinate those of Corbicula sandai (Heterodonta). The specificity of the agglutination was examined by an inhibition test using various carbohydrates, from which it was concluded that an antigenic determinant is GlcA4Me-Fuc. Immunohistochemical studies indicated that Lipid IV exists on the cell surface of the spermatozoa.

journal_name

J Biochem

journal_title

Journal of biochemistry

authors

Sugita M,Inoue T,Itasaka O,Hori T

doi

10.1093/oxfordjournals.jbchem.a134664

subject

Has Abstract

pub_date

1984-03-01 00:00:00

pages

737-42

issue

3

eissn

0021-924X

issn

1756-2651

journal_volume

95

pub_type

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