Induction of four heat-shock proteins and their mRNAs in rat after whole-body hyperthermia.

abstract：:The activities of the digestive enzymes, maltase [EC 3.2.1.20], sucrase [EC 3.2.1.26], trehalase [EC 3.2.1.28], Leucine aminopeptidase [EC 3.4.11.1], and alkaline phosphatase [EC 3.1.3.1] were measured in various regions of the small intestine of rats. The activities of all these enzymes were much higher in the jejunu...

journal_title：Journal of biochemistry

authors： Saito M,Murakami E,Nishida T,Fujisawa Y,Suda M

更新日期：1975-09-01 00:00:00

abstract：:A high mobility group (HMG) nonhistone protein fraction HMG(1 + 2), composed of HMG1 and HMG2, was prepared from pig thymus chromatin. In order to examine a possibility that the HMG(1 + 2) participates in the unwinding of the DNA double-helix, DNA hydrolysis assay systems with the endonucleases specific for single-str...

journal_title：Journal of biochemistry

authors： Yoshida M,Shimura K

更新日期：1984-01-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:Thyrotropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the TRH-induced hydrolysis of PIP2, the conversion of phosphatidylinositol 4-phosphate (PIP) to PIP2 and the accumulation...

journal_title：Journal of biochemistry

authors： Suzuki R,Takenawa T

更新日期：1987-09-01 00:00:00

abstract：:Histidinoalanine, a cross-linking component of connective tissue proteins, was detected in the acid hydrolysate of human urine. The concentrations in urines from newborn babies, children, and adults were 1.33 +/- 0.27, 0.77 +/- 0.23, and 0.89 +/- 0.33 nmol/mg creatinine, respectively. Possible origins of urinary histi...

journal_title：Journal of biochemistry

authors： Fujimoto D

更新日期：1986-09-01 00:00:00

abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...

journal_title：Journal of biochemistry

authors： Yamamoto K,Honjo R,Sekine T

更新日期：1980-01-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:The experimental conditions for release of the regulatory light chain (RLC) of scallop myosin at 30 degrees C were studied. Substantially all RLC was released from myosin by incubation for 5 min in medium containing buffer and KCl. This release of RLC was inhibited strongly by Ca2+, while the effect of Mg2+ was about ...

journal_title：Journal of biochemistry

authors： Asakawa T,Azuma N

更新日期：1983-08-01 00:00:00

abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...

journal_title：Journal of biochemistry

authors： Karasaki Y,Ohno M

更新日期：1978-09-01 00:00:00

abstract：:Rp-cAMPS, the Rp-diastereomer of adenosine 3',5'-phosphorothioate, is often referred to as a cAMP antagonist, since it binds to the regulatory subunit of cAMP-dependent protein kinase without dissociation of free catalytic subunits. To evaluate the role of cAMP-dependent protein kinase in amylase exocytosis, we examin...

journal_title：Journal of biochemistry

authors： Takuma T,Ichida T

更新日期：1991-08-01 00:00:00

abstract：:We previously reported that DNAs directly applicable to restriction analyses and transformation of Escherichia coli can be extracted from fungi and yeasts by use of anhydrous hydrogen fluoride (HF) under a mild condition, 5 min at 0 degrees C [Oshiro, S., Katsura, N., Kitada, K., and Gunge, N. (1987) FEBS Lett. 220, 3...

journal_title：Journal of biochemistry

authors： Oshiro S,Katsura N

更新日期：1995-03-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 micrograms/mg DNA, which corresponds ...

journal_title：Journal of biochemistry

authors： Tamura H,Sekimizu K,Obinata M,Natori S

更新日期：1980-11-01 00:00:00

abstract：:Three kinds of phospholipase C [EC 3.1.4.3] were used to selectively hydrolyze phospholipids in rat liver microsomes, and their effects on the acyl-CoA: glycerophosphate and acyl-CoA: lysophospholipids acyltransferase systems were examined. The glycerophosphate acyltransferase [EC 2.3.1.15] system was inactivated rapi...

journal_title：Journal of biochemistry

authors： Sonoki S,Ikezawa H

更新日期：1976-12-01 00:00:00

abstract：:Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

journal_title：Journal of biochemistry

authors： Mikami B,Nomura K,Morita Y

更新日期：1983-07-01 00:00:00

abstract：:A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the att...

journal_title：Journal of biochemistry

authors： Miyagawa A,Okuyama T

更新日期：1977-06-01 00:00:00

abstract：:We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1-0.01 m...

journal_title：Journal of biochemistry

authors： Nagino K,Nomura O,Takii Y,Myomoto A,Ichikawa M,Nakamura F,Higasa M,Akiyama H,Nobumasa H,Shiojima S,Tsujimoto G

更新日期：2006-04-01 00:00:00

abstract：:Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

journal_title：Journal of biochemistry

authors： Tsuda H,Takeshige K,Shibata Y,Minakami S

更新日期：1984-05-01 00:00:00

abstract：:Protein tyrosine phosphatase receptor type Z (PTPRZ, also known as PTPζ or RPTPβ) is preferentially expressed in the central nervous system (CNS). PTPRZ plays important roles during development and adulthood in CNS myelination, learning and memory. Three splicing isoforms for PTPRZ have been identified to date: two re...

journal_title：Journal of biochemistry

authors： Fujikawa A,Chow JPH,Matsumoto M,Suzuki R,Kuboyama K,Yamamoto N,Noda M

更新日期：2017-11-01 00:00:00

abstract：:C-Proteins in developing, denervated, and dystrophic chicken skeletal muscles were examined by means of two-dimensional (2D) gel electrophoresis in combination with immunoblotting. In this analysis, the electrophoresis system which was devised by Hirabayashi (Anal. Biochem. 117, 443-451, 1981) provided excellent resol...

journal_title：Journal of biochemistry

authors： Kojima T,Sano K,Hirabayashi T,Obinata T

更新日期：1990-03-01 00:00:00

abstract：:Human serum was shown to contain N-acetyllactosamine: N-acetylglucosaminyltransferase activity. The reaction product was hydrolyzed by beta-N-acetylglucosaminidase and released [14C]N-acetylglucosamine, indicating that the N-acetylglucosaminyl residue was beta-linked to N-acetyllactosamine. Methylation and hydrolysis ...

journal_title：Journal of biochemistry

authors： Hosomi O,Takeya A,Kogure T

更新日期：1984-06-01 00:00:00

abstract：:Skeletal muscle is composed of multinuclear cells called myofibers. Muscular dystrophy (a genetic muscle disorder) induces instability in the cell membrane of myofibers and eventually causes myofibre damage. Non-genetic muscle disorders, including sarcopenia, diabetes, bedridden immobility and cancer cachexia, lead to...

journal_title：Journal of biochemistry

authors： Fukada SI

更新日期：2018-05-01 00:00:00

abstract：:Chalcone synthase was purified to homogeneity by polyacrylamide gel electrophoresis from cell suspension cultures of carrot in which anthocyanin synthesis was induced by transferring the cells from a medium containing 2,4-dichlorophenoxy-acetic acid (2,4-D) to one lacking it. A molecular weight of 80,000-85,000 for th...

journal_title：Journal of biochemistry

authors： Ozeki Y,Sakano K,Komamine A,Tanaka Y,Noguchi H,Sankawa U,Suzuki T

更新日期：1985-07-01 00:00:00

abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...

journal_title：Journal of biochemistry

authors： Yohda M,Yoshida M

更新日期：1987-10-01 00:00:00

abstract：:Picolinate binds to a reduced form of D-amino acid oxidase, and the complex formed has a broad absorption band around 600 nm as in the case of the purple intermediate of the enzyme with a substrate. The dissociation constant at 25 degrees C was 35 microns at pH 7.0. The pH dependence (pH 8.3-pH 6.4) of the dissociatio...

journal_title：Journal of biochemistry

authors： Nishina Y,Tojo H,Shiga K

更新日期：1986-03-01 00:00:00

abstract：:A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...

journal_title：Journal of biochemistry

authors： Kinoshita M,Okazaki M,Kato H,Teramoto T,Matsushima T,Naito C,Oka H,Hara I

更新日期：1983-08-01 00:00:00

abstract：:The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and fluorescence spectroscopy. 1. T2A was mainly composed of unordered structure, although the molecule was folded into a rat...

journal_title：Journal of biochemistry

authors： Suzuki K,Ohno S,Imahori K

更新日期：1980-03-01 00:00:00

abstract：:We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

journal_title：Journal of biochemistry

authors： You J,Kamihira M,Iijima S

更新日期：1999-06-01 00:00:00