Human serum contains N-acetyllactosamine: beta 1-3 N-acetylglucosaminyltransferase activity.

abstract：:The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous w...

journal_title：Journal of biochemistry

authors： Yoon SS,Park SH,Kim YC,Shin M,Chong CK,Choi JD

更新日期：2008-10-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and cha...

journal_title：Journal of biochemistry

authors： Kim S,Oh DB,Kwon O,Kang HA

更新日期：2010-04-01 00:00:00

abstract：:Rhizopus delemar (ATCC 34612) lipase was modified by phospholipid (PL)-treatment so as to enhance its activity on lipoprotein. In order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove PL from the PL-treated lipase solution which included PL nonessential t...

journal_title：Journal of biochemistry

authors： Iwai M,Shimada Y,Tsujisaka Y

更新日期：1980-08-01 00:00:00

abstract：:When meso-tetrakis(3-N-methylpyridiniumyl)porphyrin (m-TMPyP) formed a complex with poly[d(A-T)(2)], an intense bisignate excitonic CD in the Soret absorption region was observed. The excitonic CD of the m-TMPyP-poly[d(A-T)(2)] complex is unique in that no other combination of the related porphyrin, namely, meso-tetra...

journal_title：Journal of biochemistry

authors： Lee YA,Lee S,Lee HM,Lee CS,Kim SK

更新日期：2003-03-01 00:00:00

abstract：:Sugars such as glycolaldehyde, glyceraldehyde, erythrose, ribose, and glucose were heated with ammonia in a modified sea medium. Amino acids such as glycine, alanine, serine, threonine, aspartic acid, and glutamic acid were obtained from the reaction mixture. This provides the basis for a model of the genesis of amino...

journal_title：Journal of biochemistry

authors： Yanagawa H,Kobayashi Y,Egami F

更新日期：1980-01-01 00:00:00

abstract：:Low molecular weight RNAs were isolated from nuclei of the cellular slime mold Dictyostelium discoideum AX-3. Analysis of the RNAs by polyacrylamide gel electrophoresis showed that the vegetative cell nuclei contained, besides tRNA (Dd1), 5S RNA (Dd4), and 5.8S RNA (Dd7), at least 7 small RNA species (Dd3, Dd5, Dd6, D...

journal_title：Journal of biochemistry

authors： Takeishi K,Kaneda S

更新日期：1981-08-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:The whole-genome sequencing of an extreme thermophile, Thermus thermophilus, is now in progress. Like other genome projects, major concern is shifting from the sequence itself to post-sequencing research such as functional or structural genomics. Under such circumstances, the demand for convenient genetic-engineering ...

journal_title：Journal of biochemistry

authors： Hoseki J,Yano T,Koyama Y,Kuramitsu S,Kagamiyama H

更新日期：1999-11-01 00:00:00

abstract：:The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

journal_title：Journal of biochemistry

authors： Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

更新日期：1993-05-01 00:00:00

abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

journal_title：Journal of biochemistry

authors： Maeng JH,Yoon JB

更新日期：1998-07-01 00:00:00

abstract：:The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In ...

journal_title：Journal of biochemistry

authors： Takiya S,Saito S,Yokoyama T,Matsumoto D,Aizawa T,Kamiya M,Demura M,Kawano K

更新日期：2009-07-01 00:00:00

abstract：:Beta-N-Acetylgalactosaminidase [EC 3.2.1.53] was purified to homogeneity from the culture media of Bacillus sp. AT173-1. The enzyme has a molecular weight of 48,000 as estimated by SDS-PAGE under reducing conditions and an isoelectric point of 4.3. The enzyme requires dithiothreitol as an activator and is most active ...

journal_title：Journal of biochemistry

authors： Tanaka A,Ozaki S

更新日期：1997-08-01 00:00:00

abstract：:N omega-Phosphoarginine hydrolase from rat liver cytosol was purified to apparent homogeneity on SDS-PAGE, by employing column chromatographies on Sephadex G-75, DEAE-cellulose, QAE-Toyopearl, and glutathione-2-pyridyl-disulfide-Superose. One milligram protein of the final preparation released 4 mumol/min of inorganic...

journal_title：Journal of biochemistry

authors： Yokoyama K,Ohmori H,Kumon A

更新日期：1993-02-01 00:00:00

abstract：:beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

journal_title：Journal of biochemistry

authors： D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

更新日期：1999-09-01 00:00:00

abstract：:Induction of the enzymes involved in fatty acid beta-oxidation in Pseudomonas fragi B-0771 cells grown in a medium containing straight chain saturated fatty acids was studied. The acyl-CoA dehydrogenase (ACDH) activity was induced during the exponential phase in cells grown in palmitic acid-supplemented medium, reache...

journal_title：Journal of biochemistry

authors： Sato S,Imamura S,Ozeki Y,Kawaguchi A

更新日期：1992-01-01 00:00:00

abstract：:We found that transducing phages carrying the gal or bio regions of the Escherichia coli genome were formed during in vitro packaging of endogenous lambda DNA. Structural analysis of the transducing phage genomes indicated that they were formed by abnormal excision of lambda prophage. Formation of transducing phages w...

journal_title：Journal of biochemistry

authors： Tomono M,Shiozaki M,Ikeda H

更新日期：1989-03-01 00:00:00

abstract：:Telomerase is a unique reverse transcriptase involved in the maintenance of telomeric DNA, which is generally undetectable in normal human somatic cells. However, it has been found in organs of normal adult rodents including the liver. In order to elucidate relevant control mechanisms operating in normal somatic cells...

journal_title：Journal of biochemistry

authors： Nozawa K,Kurumiya Y,Yamamoto A,Isobe Y,Suzuki M,Yoshida S

更新日期：1999-08-01 00:00:00

abstract：:The absorption and fluorometric changes of the reaction mixture of luciferase-FMNH2 complex with O2 were re-examined. Rapid formation (k2(app) = 2.0 s-1 at [O2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than o...

journal_title：Journal of biochemistry

authors： Ashizawa N,Nakamura T,Watanabe T

更新日期：1977-04-01 00:00:00

abstract：:To better understand the regulation of ryudocan (syndecan-4) expression, we have determined the structural organization of the human ryudocan gene. The human ryudocan gene extends approximately 24 kilobases and is divided into five exons, which appear to be conserved in syndecan family members. Exon I encodes the sign...

journal_title：Journal of biochemistry

authors： Takagi A,Kojima T,Tsuzuki S,Katsumi A,Yamazaki T,Sugiura I,Hamaguchi M,Saito H

更新日期：1996-05-01 00:00:00

abstract：:The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-beta-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatograp...

journal_title：Journal of biochemistry

authors： Liang CJ,Yamashita K,Kobata A

更新日期：1980-07-01 00:00:00

abstract：:The interactions of porcine alpha2-macroglobulin (alpha2M) with native proteinases, their zymogens and the chemically-modified enzymes were compared. The alpha2M did not bind to chymotrypsinogen, or to most of the chemically modified derivatives of alpha-chymotrypsin, trypsinogen, DIP- and PMS-trypsins, but it could i...

journal_title：Journal of biochemistry

authors： Tsuru D,Kado K,Fujiwara K,Tmimatsu M,Ogita K

更新日期：1978-05-01 00:00:00

abstract：:An inorganic pyrophosphatase [EC 3.6.1.1] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum acti...

journal_title：Journal of biochemistry

authors： Tominaga N,Mori T

更新日期：1977-02-01 00:00:00

abstract：:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. Th...

journal_title：Journal of biochemistry

authors： Uchikoba T,Yonezawa H,Kaneda M

更新日期：1995-05-01 00:00:00

abstract：:Calmodulin-dependent protein kinase IV (CaM-kinase IV) kinase was recently discovered in the rat brain by its activity to activate the inactive recombinant CaM-kinase IV expressed in Escherichia coli [Okuno, S. and Fujisawa, H. (1993) J. Biochem. 114, 167-170]. In the present study, CaM-kinase IV kinase was purified a...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：1994-10-01 00:00:00

abstract：:Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the delta pH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dic...

journal_title：Journal of biochemistry

authors： Moriyama Y,Takano T,Ohkuma S

更新日期：1982-10-01 00:00:00

abstract：:p-Nitrophenyl O-6-deoxy-6-[(2-pyridyl)amino]-alpha-D-glucopyranosyl-(1----4)-O-alpha- D-glucopyranosyl-(1----4)-O-alpha-D-glucopyranosyl-(1----4)-O-alpha-D- glucopyranosyl-(1----4)-alpha-D-glucopyranoside (FG5P) is hydrolyzed by human pancreatic a-amylase (HPA) or salivary alpha-amylase (HSA) to O-6-deoxy-6-[(2-pyridy...

journal_title：Journal of biochemistry

authors： Omichi K,Ikenaka T

更新日期：1986-11-01 00:00:00

abstract：:The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein of 286 amino acids. The amino acid sequence shared approximately 80-90% similarity to the Bacillus ...

journal_title：Journal of biochemistry

authors： Ertunga NS,Colak A,Belduz AO,Canakci S,Karaoglu H,Sandalli C

更新日期：2007-06-01 00:00:00

abstract：:The gizzard tropomyosin molecule is composed of two subunits at 1:1 molar ratio. Possible composites of the tropomyosin molecule are two kinds of homodimer (one for each subunit), a heterodimer of two subunits, or a mixture of heterodimer and homodimer(s). We tried to evaluate the native subunit composition of gizzard...

journal_title：Journal of biochemistry

authors： Nakamura S,Sakurai T,Nonomura Y

更新日期：1991-05-01 00:00:00