Cloning, expression, purification and characterization of fructose-1,6-bisphosphate aldolase from Anoxybacillus gonensis G2.

abstract：:Controlled chymotryptic digestion of the 165,000 dalton component of the M-line of rabbit skeletal muscle, followed by Biogel P-150 chromatography of the digest, has led to the isolation of a homogeneous 100,000 dalton species. This fragment was found, by both sedimentation equilibrium and gel filtration chromatograph...

journal_title：Journal of biochemistry

authors： Mani RS,Kay CM

更新日期：1979-12-01 00:00:00

abstract：:Effects of rice bran agglutinin (RBA) on human monoblastic leukemia U937 cells were examined in comparison with those of wheat germ agglutinin (WGA) and Viscum album agglutinin (VAA). These lectins inhibit cell growth, and several lines of evidence indicate that the growth inhibition is caused by the induction of apop...

journal_title：Journal of biochemistry

authors： Miyoshi N,Koyama Y,Katsuno Y,Hayakawa S,Mita T,Ohta T,Kaji K,Isemura M

更新日期：2001-12-01 00:00:00

abstract：:The in vivo effects of convulsant drugs (hydrazine and penicillamine) on the metabolism of gamma-aminobutyric acid (GABA) in subcellular fractions of mouse brain were studied. Both substances inhibited the activity of glutamic acid decarboxylase [EC 4.1.1.15] (GAD) in the synaptosomal fraction (nerve ending particles)...

journal_title：Journal of biochemistry

authors： Abe M,Matsuda M

更新日期：1977-07-01 00:00:00

abstract：:The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

journal_title：Journal of biochemistry

authors： Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

更新日期：1985-11-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00

abstract：:A method is described for the fractionation of reduced and alkylated proteins of fowl feather. Fowl feather extracts were chromatographed on a Sephadex G-75 column in 4 M urea containing 1 M NaCl and separated into four fractions, GF-1, 2, 3, and 4. The elution patterns were used to compare the components of different...

journal_title：Journal of biochemistry

authors： Akahane K,Murozono S,Murayama K

更新日期：1977-01-01 00:00:00

abstract：:The plant hormone ethylene is generated from a unique precursor, 1-aminocyclopropane-1-carboxylate (ACC). In previous studies, ACC deaminase, which degrades ACC to alpha-ketobutyrate and ammonia, was found in four strains of Pseudomonas, characterized, and sequenced. To verify the wider distribution of ACC deaminase i...

journal_title：Journal of biochemistry

authors： Minami R,Uchiyama K,Murakami T,Kawai J,Mikami K,Yamada T,Yokoi D,Ito H,Matsui H,Honma M

更新日期：1998-06-01 00:00:00

abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

journal_title：Journal of biochemistry

authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1993-06-01 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:Colicin E5 cleaves tRNAs for Tyr, His, Asn and Asp in their anticodons to abolish protein synthesis in Escherichia coli. We previously showed how its C-terminal RNase domain, E5-CRD, recognizes the anticodon bases but the catalytic mechanism remained to be elucidated. Although the reaction products with 5'-OH and 2',3...

journal_title：Journal of biochemistry

authors： Inoue-Ito S,Yajima S,Fushinobu S,Nakamura S,Ogawa T,Hidaka M,Masaki H

更新日期：2012-10-01 00:00:00

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

journal_title：Journal of biochemistry

authors： Miyazawa K,Mori A,Okudaira H

更新日期：1998-12-01 00:00:00

abstract：:1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

journal_title：Journal of biochemistry

authors： Kohama K

更新日期：1979-09-01 00:00:00

abstract：:In our previous study, the sphingosine-like immunosuppressant ISP-1 was shown to induce apoptosis in the mouse cytotoxic T cell line CTLL-2. In this study, we characterized the ISP-1-induced apoptotic pathway. Although caspase-3-like protease activity increases concomitantly with ISP-1-induced apoptosis in CTLL-2 cell...

journal_title：Journal of biochemistry

authors： Yamaji T,Nakamura S,Takematsu H,Kawasaki T,Kozutsumi Y

更新日期：2001-04-01 00:00:00

abstract：:The SoxR protein of Escherichia coli responds to redox signals by activating the transcription of soxS, which encodes another transcription activator that directly stimulates oxidative stress genes. In contrast, Pseudomonas aeruginosa has an open reading frame (ORF) encoding a putative protein homologous to E. coli So...

journal_title：Journal of biochemistry

authors： Kobayashi K,Tagawa S

更新日期：2004-11-01 00:00:00

abstract：:Recombinant human granulocyte-colony-stimulating factor (G-CSF) was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA. The recombinant human G-CSF was treated with alkaline borohydride and the oligosaccharide-alditols liberated were fractioned by gel filtration on a Bio-Gel P-4 column, follow...

journal_title：Journal of biochemistry

authors： Oheda M,Hase S,Ono M,Ikenaka T

更新日期：1988-03-01 00:00:00

abstract：:1. A major glucoamylase [EC 3.2.1.3] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

journal_title：Journal of biochemistry

authors： Takahashi T,Inokuchi N,Irie M

更新日期：1981-01-01 00:00:00

abstract：:In this study, we demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phosphatidylserine (PS)-binding protein and localized the putative PS-binding site involved in the membrane fusion induced by this enzyme. In an attempt to identify the PS-binding proteins, we raised polyclonal antibodies against...

journal_title：Journal of biochemistry

authors： Kaneda M,Takeuchi K,Inoue K,Umeda M

更新日期：1997-12-01 00:00:00

abstract：:Hyperthermophilic bacteria Thermotoga maritima and Thermotoga hypogea produce ethanol as a metabolic end product, which is resulted from acetaldehyde reduction catalysed by an alcohol dehydrogenase (ADH). However, the enzyme that is involved in the production of acetaldehyde from pyruvate is not well characterized. An...

journal_title：Journal of biochemistry

authors： Eram MS,Wong A,Oduaran E,Ma K

更新日期：2015-12-01 00:00:00

abstract：:Previously, we demonstrated that the 2.6-kDa peptide extending from Arg177 to Tyr198 in subdomain 4 of rabbit skeletal actin bound to actin itself, inhibited the elongation of actin filament, and severed F-actin. The corresponding segment in actin, therefore, is thought to contain the most critical actin-actin contact...

journal_title：Journal of biochemistry

authors： Hori K,Morita F,Matsuzawa F,Aikawa S

更新日期：1995-12-01 00:00:00

abstract：:Substrate specificities of phospholipases C[EC 3.1.4.3] from Clostridium novyi, Clostridium perfringens, Bacillus cereus, and Pseudomonas aureofaciens were studied under the same conditions. Phospholipases C from Clostridium novyi and Bacillus cereus show wide substrate specificities while those of Clostridium perfrin...

journal_title：Journal of biochemistry

authors： Taguchi R,Ikezawa H

更新日期：1977-11-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Calpastatin, an endogenous inhibitor protein specifically acting on calpain [EC 3.4.22.17; Ca2+-dependent cysteine proteinase], was purified to apparent homogeneity from the cytosol fraction of human erythrocytes. The yield was 0.38 mg from 400 ml of blood. The purification procedures included DEAE-cellulose and Ultro...

journal_title：Journal of biochemistry

authors： Takano E,Murachi T

更新日期：1982-12-01 00:00:00

abstract：:The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...

journal_title：Journal of biochemistry

authors： Kikuchi K,Umehara T,Fukuda K,Hwang J,Kuno A,Hasegawa T,Nishikawa S

更新日期：2003-03-01 00:00:00

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:A method for preparation of pyridylamino (PA-) derivatives of O-linked sugar chains from glycoproteins was developed. A glycopeptide containing O-linked Gal beta 1-3GalNAc was prepared from fetuin and treated with anhydrous hydrazine followed by N-acetylation of free amino groups. Sugar chains released were pyridylami...

journal_title：Journal of biochemistry

authors： Kuraya N,Hase S

更新日期：1992-07-01 00:00:00

abstract：:Neuronal synapse formation is regulated by pre- and postsynaptic cell adhesion molecules. Presynaptic neurexins (NRXNs) and receptor protein tyrosine phosphatases (RPTPs; PTPδ, PTPσ and LAR in mammals) can induce postsynaptic differentiation through the interaction with various postsynaptic cell adhesion molecules. He...

journal_title：Journal of biochemistry

authors： Uemura T,Shiroshima T,Maeda A,Yasumura M,Shimada T,Fukata Y,Fukata M,Yoshida T

更新日期：2017-10-01 00:00:00

abstract：:The lifetime of the purple intermediate formed from D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3] and a neutral D-amino acid under anaerobic conditions was measured with a series of neutral D-amino acids. The lifetime increases with increase in the number of carbons in the side chain...

journal_title：Journal of biochemistry

authors： Yagi K,Ohishi N

更新日期：1978-12-01 00:00:00

abstract：:The DNA fragment containing the phoS gene, a regulatory gene for alkaline phosphatase, has been isolated from Escherichia coli K-12 chromosomal DNA by cutting off the DNA with Hind III restriction enzyme and by cloning the gene with plasmid vector pTP 4 which was constructed in this study. The isolated fragment was of...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Tsuda K

更新日期：1982-09-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00