In situ screening for postsynaptic cell adhesion molecules during synapse formation.


:Neuronal synapse formation is regulated by pre- and postsynaptic cell adhesion molecules. Presynaptic neurexins (NRXNs) and receptor protein tyrosine phosphatases (RPTPs; PTPδ, PTPσ and LAR in mammals) can induce postsynaptic differentiation through the interaction with various postsynaptic cell adhesion molecules. Here, we developed a novel in situ screening method to identify postsynaptic membranous proteins involved in synaptogenesis. Magnetic beads coated with the extracellular domains of NRXN1β(-S4) and PTPδ-A6 variants preferentially induced excitatory postsynaptic differentiation on the beads' surface when co-cultured with cortical neurons. After inducing postsynaptic sites on these beads, protein complexes including NRXN1β(-S4)/PTPδ-A6 and their ligands on the neuronal membrane were chemically cross-linked and purified using a magnetic separator. Liquid chromatography-tandem mass spectrometry analysis of the complexes revealed two types of postsynaptic ligands for NRXN1β(-S4) and PTPδ-A6, one has an activity to induce presynaptic differentiation in a trans manner, whereas the other has no such activity. These results suggest that synapse formation is regulated by the interplay between presynaptic NRXN/PTPδ and their postsynaptic ligands with functionally different impacts on pre- and postsynaptic differentiation. Thus, our in situ screening method for identifying synapse-organizing complexes will help to understand the molecular basis for elaborate neuronal networks.


J Biochem


Journal of biochemistry


Uemura T,Shiroshima T,Maeda A,Yasumura M,Shimada T,Fukata Y,Fukata M,Yoshida T




Has Abstract


2017-10-01 00:00:00














  • Demonstration of the importance and usefulness of manipulating non-active-site residues in protein design.

    abstract::Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

    更新日期:2001-06-01 00:00:00

  • Antigenic interspecies cross-reaction of subcomponent C1q of the first component of complement: evidence for its cross-reaction in both collagen-like and non-collagen-like regions.

    abstract::Human, bovine, and mouse C1q, a subcomponent of the first complement component, were purified, and both globular (GF) and collagen-like fragments (CLF) were isolated from human and bovine C1q. Antisera were produced in rabbits with these C1q or fragments, and F(ab')2 of immunoglobulin G (IgG) was purified from the ant...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yonemasu K,Sasaki T,Shimizu A

    更新日期:1983-04-01 00:00:00

  • Isoelectrical focusing of connectin by agarose gel electrophoresis.

    abstract::Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsumura K,Hori S,Sugiura H,Shimizu T

    更新日期:1990-10-01 00:00:00

  • Detection of novel metabolite for Roxadustat doping by global metabolomics.

    abstract::Roxadustat (FG-4592, Rox) is a stabilizer for hypoxia-inducible transcription factors (HIFs), which induce production of the erythroid growth factor erythropoietin, and has been listed by the World Anti-Doping Agency as a prohibited substance for athletes since 2011. Although the detection technologies for Rox and its...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saigusa D,Suzuki N,Matsumoto Y,Umeda K,Tomioka Y,Koshiba S,Yamamoto M

    更新日期:2018-02-09 00:00:00

  • Rat cytosolic aspartate aminotransferase: regulation of its mRNA and contribution to gluconeogenesis.

    abstract::Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

    更新日期:1988-05-01 00:00:00

  • Involvement of free ribosomes in the early stage of secretory protein biosynthesis in rat liver.

    abstract::Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin na...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda T,Ito A

    更新日期:1981-05-01 00:00:00

  • Protein phosphatase 2A inhibitors, phoslactomycins. Effects on the cytoskeleton in NIH/3T3 cells.

    abstract::Protein phosphorylation is a key regulatory mechanism of the organization and dynamics of the actin cytoskeleton during cell motility, differentiation, and cytokinesis. The level of protein phosphorylation is dependent on the relative activities of both protein kinases and protein phosphatases. In this paper, we exami...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Usui T,Marriott G,Inagaki M,Swarup G,Osada H

    更新日期:1999-05-01 00:00:00

  • Preparation of monoclonal antibodies against N-(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated acetylspermine, and development of an enzyme-linked immunosorbent assay (ELISA) for N1,N12-diacetylspermine.

    abstract::We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2b, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(gamma-maleimidobutyryloxy...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujiwara K,Kaminishi Y,Kitagawa T,Tsuru D,Yabuuchi M,Kanetake H,Nomata K

    更新日期:1998-07-01 00:00:00

  • Alpha-2-macroglobulin-like protease inhibitor from the egg white of cuban crocodile (Crocodylus rhombifer).

    abstract::A high molecular weight protease inhibitor was purified from the egg white of Cuban crocodile (Crocodylus rhombifer). It inhibited the casein hydrolyzing activity of trypsin, subtilisin and papain. Its native molecular weight was 730,000 and it consisted of four subunits of equal molecular weight, each pair of which w...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ikai A,Kitamoto T,Nishigai M

    更新日期:1983-01-01 00:00:00

  • A cholesterol-independent membrane microdomain serves as a functional counter-receptor for E-selectin at the Colo201 cell surface and initiates signalling on E-selectin binding.

    abstract::The present study demonstrates that the functional counter-receptors for E-selectin at the cell surface of Colo201 human colon cancer cells are localized in detergent-insoluble membrane microdomains (DIM). Following isolation of counter-receptors from whole cell lysates using E-selectin-coupled magnetic beads followed...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suzuki C,Kojima N

    更新日期:2007-07-01 00:00:00

  • Nucleotide sequence analysis of human beta-globin gene by the quantification method: mutations in 3'-splice junction sequence and beta-thalassemia.

    abstract::The nucleotide sequence at the intron-exon junction in the human beta-globin gene was analyzed by the quantification method (categorical discriminant analysis) proposed previously. Using the sample score of a 16-nucleotide sequence at a 3'-splice junction, we studied to what extent such a sequence contains the 3'-spli...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iida Y

    更新日期:1990-12-01 00:00:00

  • Specific affinity of glycerol dehydrogenase from Geotrichum candidium for 10-carboxydecyl-sepharose: its application to chromatography for purification of the enzyme.

    abstract::Glycerol dehydrogenase [EC] (pI 5.9) from Geotrichum candidium is effectively adsorbed in the presence of 20 mM acute buffer (pH 6.0) on either octyl-Sepharose or 10-carboxydecyl-Sepharose, among ten different kinds of n-alkyl-Sepharose derivatives tested, some of which have an amino or a carboxyl group as a t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sasaki I,Itoh N,Goto H,Yamamoto R,Tanaka H,Yamashita K,Yamashita J,Horio T

    更新日期:1982-01-01 00:00:00

  • A novel insertion sequence transposed to thermophilic bacteriophage {phi}IN93.

    abstract::The nucleotide sequence of IStaqTZ2 are available in the DDBJ/EMBL/GenBank databases under the accession number AB063392. A novel insertion sequence (IStaqTZ2) was transposed from the genome of Thermus thermophilus TZ2 to that of the thermophilic bacteriophage IN93. The complete nucleotide sequence of IStaqTZ2 was det...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsushita I,Yanase H

    更新日期:2009-12-01 00:00:00

  • Light-induced pH changes and changes in absorbance of pH indicators in Rhodospirillum rubrum chromatophores.

    abstract::1. The light-induced pH change of chromatophore suspensions from Rhodospirillum rubrum was stimulated significantly and similarly by KCl, NaCl, LiCl, RbCl, CsCl, MgCl2, MnCl2, and CaCl2. In the dark, the pH of chromatophore suspensions decreased immediately and markedly on adding these salts. 2. The light-induced pH c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishi N,Sakata-Sogawa K,Soe G,Yamashita J

    更新日期:1977-11-01 00:00:00

  • Identification of antigenic regions of the Erns protein for pig antibodies elicited during classical swine fever virus infection.

    abstract::The structural glycoprotein E(rns) of classical swine fever virus (CSFV) is one of the major antibody targets upon infection of pigs with the virus. Molecular dissection of the structure of E(rns) would define the minimal immunodominant regions that induce antibody responses after infection and may thus help design an...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Lin M,Trottier E,Pasick J,Sabara M

    更新日期:2004-12-01 00:00:00

  • Two distinct ferredoxins from Rhodobacter capsulatus: complete amino acid sequences and molecular evolution.

    abstract::Two distinct ferredoxins were purified from Rhodobacter capsulatus SB1003. Their complete amino acid sequences were determined by a combination of protease digestion, BrCN cleavage and Edman degradation. Ferredoxins I and II were composed of 64 and 111 amino acids, respectively, with molecular weights of 6,728 and 12,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saeki K,Suetsugu Y,Yao Y,Horio T,Marrs BL,Matsubara H

    更新日期:1990-09-01 00:00:00

  • Characterization of recombinant YakC of Schizosaccharomyces pombe showing YakC defines a new family of aldo-keto reductases.

    abstract::The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morita T,Huruta T,Ashiuchi M,Yagi T

    更新日期:2002-10-01 00:00:00

  • Reassembly of nucleosomal histone octamers during replication of chromatin.

    abstract::We have examined critically whether or not new and old histones mix in the octameric units of nucleosomes during chromatin replication. MH-134SC cells were density-labeled by culturing with amino acid mixtures enriched with dense isotopes 2H, 13C, and 15N. Mononucleosomes obtained from labeled cells were fractionated ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamasu K,Senshu T

    更新日期:1987-04-01 00:00:00

  • Establishment of glutamine synthetase of Mycobacterium smegmatis as a protein acetyltransferase utilizing polyphenolic acetates as the acetyl group donors.

    abstract::Acetoxy Drug: Protein Transacetylase (TAase) mediating the transfer of acetyl group(s) from polyphenolic acetates (PA) to certain functional proteins in mammalian cells was identified by our earlier investigations. TAase activity was characterized in the cell lysates of Mycobacterium smegmatis and the purified protein...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Gupta G,Baghel AS,Bansal S,Tyagi TK,Kumari R,Saini NK,Ponnan P,Kumar A,Bose M,Saluja D,Patkar SA,Parmar VS,Raj HG

    更新日期:2008-12-01 00:00:00

  • Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii.

    abstract::At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

    更新日期:2001-12-01 00:00:00

  • Catalytic role of an arginine residue in the highly conserved and unique sequence of phosphoenolpyruvate carboxylase.

    abstract::Phosphoenolpyruvate carboxylase (PEPC) [EC] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yano M,Terada K,Umiji K,Izui K

    更新日期:1995-06-01 00:00:00

  • Isolation of DNA fragment containing phoS gene of Escherichia coli K-12.

    abstract::The DNA fragment containing the phoS gene, a regulatory gene for alkaline phosphatase, has been isolated from Escherichia coli K-12 chromosomal DNA by cutting off the DNA with Hind III restriction enzyme and by cloning the gene with plasmid vector pTP 4 which was constructed in this study. The isolated fragment was of...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwakura M,Shimura Y,Tsuda K

    更新日期:1982-09-01 00:00:00

  • Regulation of Src family kinases in the developing rat brain: correlation with their regulator kinase, Csk.

    abstract::We have so far suggested that the functions of Src family protein-tyrosine kinases are under the control of their regulator kinase, Csk. To evaluate the role of Csk-mediated regulation in neural tissues, we examined the correlation between the activities of Src family kinases and the expression level of Csk during dev...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inomata M,Takayama Y,Kiyama H,Nada S,Okada M,Nakagawa H

    更新日期:1994-08-01 00:00:00

  • Mechanism-based inactivation of coenzyme B12-dependent diol dehydratase by 3-unsaturated 1,2-diols and thioglycerol.

    abstract::The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

    更新日期:2008-10-01 00:00:00

  • Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene.

    abstract::Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suhara W,Yoneyama M,Yonekawa H,Fujita T

    更新日期:1996-02-01 00:00:00

  • Static and kinetic studies on rabbit skeletal muscle troponin.

    abstract::Fluorescence titration curves of 2-[4'-iodoacetamido)anilino)naphthalene-6-sulfonic acid-labeled troponin (IAANS-labeled Tn) and troponin-1-anilinonaphthalene-8-sulfonic acid (Tn-ANS) complex indicated that the fluorescent moiety, IAANS or ANS, detects conformational change of troponin I (TnI) or Tn due to the Ca2+ bi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishio T,Iio T

    更新日期:1983-09-01 00:00:00

  • Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

    abstract::E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

    更新日期:1983-12-01 00:00:00

  • Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomyces carlsbergensis.

    abstract::Deoxyhypusine synthase catalyzes the NAD+-dependent formation of deoxyhypusine in the eIF-5A precursor protein by transferring the 4-aminobutyl moiety of spermidine. This enzyme has recently been shown to be essential for cell viability and growth of yeast [Sasaki, K., Abid, M.R., and Miyazaki, M. (1996) FEBS Lett. 38...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Abid MR,Sasaki K,Titani K,Miyazaki M

    更新日期:1997-04-01 00:00:00

  • Transformation of leukotriene D4 catalyzed by lysosomal cathepsin H of rat liver.

    abstract::Among several intracellular protease tested, cathepsin H transformed leukotriene D4 to E4 with a release of glycine in a stoichiometric quantity. Under the optimal conditions the rate of leukotriene D4 transformation by cathepsin H was about 3% of the hydrolysis rate of alpha-N-benzoyl-DL-arginine-2-naphthylamide whic...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yokota K,Shono F,Yamamoto S,Kominami E,Katunuma N

    更新日期:1983-10-01 00:00:00

  • Detection of cyclic GMP binding protein and ion channel activity in frog rod outer segments.

    abstract::In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

    更新日期:1987-08-01 00:00:00