Purification and some properties of human erythrocyte calpastatin.

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:A 1H NMR study of a selectively deuterated mouse anti-dansyl monoclonal antibody is reported. Two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy was found to be effective for establishing the connectivity between the C2-H and C4-H protons of His residues in the antibody molecule. It has been concluded ...

journal_title：Journal of biochemistry

authors： Kato K,Nishimura Y,Waelchli M,Arata Y

更新日期：1989-09-01 00:00:00

abstract：:To model gene transcription kinetics, empirical fitting with the Hill function or S-system is often used. In this study, we derived an analytical expression for gene transcription rates in a manner similar to that developed for enzyme kinetics to describe the kinetics of gene transcription mediated by dimeric transcri...

journal_title：Journal of biochemistry

authors： Yang HT,Hsu CP,Hwang MJ

更新日期：2007-08-01 00:00:00

abstract：:Laccase is an enzyme that catalyzes the oxidation of phenolic compounds by coupling the reduction of oxygen to water. While many laccases have been identified in plant and fungal species, enzymes of prokaryotic origin are poorly known. Here we report the enzymological characterization of EpoA, a laccase-like extracyto...

journal_title：Journal of biochemistry

authors： Endo K,Hayashi Y,Hibi T,Hosono K,Beppu T,Ueda K

更新日期：2003-05-01 00:00:00

abstract：:In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed ...

journal_title：Journal of biochemistry

authors： Nakayama C,Saneyoshi M

更新日期：1985-05-01 00:00:00

abstract：:The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Norioka S,Teshima T,Ikenaka T

更新日期：1987-03-01 00:00:00

abstract：:Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated plate...

journal_title：Journal of biochemistry

authors： Takuwa Y,Takuwa N,Sugimoto N

更新日期：2002-06-01 00:00:00

abstract：:We recently demonstrated, using synthetic peptides modeled on the extension peptide of malate dehydrogenase, that amino acid residues present at the proximal and distal positions relative to the cleavage site are critical determinants for the recognition of substrates by mitochondrial processing peptidase [Niidome et ...

journal_title：Journal of biochemistry

authors： Moriwaki K,Ogishima T,Ito A

更新日期：1999-11-01 00:00:00

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taur...

journal_title：Journal of biochemistry

authors： Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IM

更新日期：1990-05-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00

abstract：:Using a combined pharmacological and genetic approach, we have identified aa 260-280 in the C2 region as a critical factor in the catalytic function of protein kinase Calpha (PKCalpha). Progressive truncations from the N-terminus as well as selected internal deletion mutants were expressed in Saccharomyces cerevisiae ...

journal_title：Journal of biochemistry

authors： Rotenberg SA,Zhu J,Hansen H,Li XD,Sun XG,Michels CA,Riedel H

更新日期：1998-10-01 00:00:00

abstract：:In order to identify the b-type cytochrome involved in the nitrate reduction in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans, the b-type cytochromes in the spheroplast membranes were characterized. Difference spectra at 77K of spheroplast membranes indicated the presence of two b-type cytoc...

journal_title：Journal of biochemistry

authors： Yokota S,Urata K,Satoh T

更新日期：1984-06-01 00:00:00

abstract：:Three glucosamine-containing sphingoglycolipids were isolated from human lung tissue of a blood group-A subject. They were hexaglycosyl, pentaglycosyl, and tetraglycosyl ceramides. The hexaglycosyl ceramide exhibited blood group-A antigenicity, and the following chemical structure was proposed on the basis of sequenti...

journal_title：Journal of biochemistry

authors： Yoda Y,Makita A,Gasa S

更新日期：1982-02-01 00:00:00

abstract：:S-RNase is associated with the gametophytic self-incompatibility of flowering plants in Solanaceae and, on the basis of sequence homology, belongs to the RNase T2 family. To identify the active site residues in S-RNase, Nicotiana alata S6-RNase was studied by chemical modification. S6-RNase was inactivated with iodoac...

journal_title：Journal of biochemistry

authors： Ishimizu T,Miyagi M,Norioka S,Liu YH,Clarke AE,Sakiyama F

更新日期：1995-11-01 00:00:00

abstract：:A novel positively charged planar bilayer membrane was formed from a mixture containing 20% cationic lipid, 1,2-dioleoyloxy-3-(trimethylammonio)propane, and neutral phospholipid mixture of 56% phosphatidylethanolamine and 24% egg phosphatidylcholine. The basic properties of the bilayer were essentially the same as tho...

journal_title：Journal of biochemistry

authors： Anzai K,Masumi M,Kawasaki K,Kirino Y

更新日期：1993-10-01 00:00:00

abstract：:The nucleotide sequence of the mRNA for NADPH-cytochrome P-450 reductase from rabbit liver was determined from a full-length cDNA clone (pFP105). The clone contains 2,269 nucleotides complementary to rabbit liver reductase mRNA. The single open reading frame of 2,037 nucleotides codes for a 679-amino acid polypeptide ...

journal_title：Journal of biochemistry

authors： Katagiri M,Murakami H,Yabusaki Y,Sugiyama T,Okamoto M,Yamano T,Ohkawa H

更新日期：1986-10-01 00:00:00

abstract：:Screening of four malic enzymes--NAD-linked enzyme [EC 1.1.1.38], NAD, NADP-linked enzyme [EC 1.1.1.39], NADP-linked enzyme [EC 1.1.1.40], and D-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of Sepharose 6B column chromatography: 9 strains of enteric bacteria,...

journal_title：Journal of biochemistry

authors： Iwakura M,Tokushige M,Katsuki H,Muramatsu S

更新日期：1978-05-01 00:00:00

abstract：:This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

journal_title：Journal of biochemistry

authors： Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

更新日期：2020-03-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. The Ca2+-, EDTA-, and Mg2+-ATPase [EC 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 M KCl at pH 7.0 and 25 degrees. The Ca2+-AT...

journal_title：Journal of biochemistry

authors： Takeuchi K,Shibata N,Senda N

更新日期：1975-07-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:Kinetic parameters, Km and Vmax for the transesterification of oligouridylic acid, (Up)nU greater than p (n=0-4), by RNase A were measured spectrophotometrically at pH 7.0 and 25 degrees C. The kinetic parameters, pKm and log Vmax increased with increase in the chain length (n), and seemed to be almost constant with s...

journal_title：Journal of biochemistry

authors： Irie M,Mikami F,Monma K,Ohgi K,Watanabe H,Yamaguchi R,Nagase H

更新日期：1984-07-01 00:00:00

abstract：:F-Actin was digested with alpha-chymotrypsin in 6 M urea, and two peptide fragments from subdomain 4 of actin molecule [Kabsch, W., Mannherz, H.G., Suck, D., Pai, E.F., & Holmes K.C. (1990) Nature 347, 37-44] were purified by reverse-phase HPLC and Sephadex G-50 gel filtration. The peptide fragments were identified as...

journal_title：Journal of biochemistry

authors： Hori K,Morita F

更新日期：1992-09-01 00:00:00

abstract：:Colicin E5 cleaves tRNAs for Tyr, His, Asn and Asp in their anticodons to abolish protein synthesis in Escherichia coli. We previously showed how its C-terminal RNase domain, E5-CRD, recognizes the anticodon bases but the catalytic mechanism remained to be elucidated. Although the reaction products with 5'-OH and 2',3...

journal_title：Journal of biochemistry

authors： Inoue-Ito S,Yajima S,Fushinobu S,Nakamura S,Ogawa T,Hidaka M,Masaki H

更新日期：2012-10-01 00:00:00

abstract：:A cysteine protease inhibitor with an apparent Mr = 12,600, designated limulus (L)-cystatin, was isolated from hemocyte lysates of the Japanese horseshoe crab (Tachypleus tridentatus), using two steps of chromatography, including dextran sulfate-agarose, and carboxymethylated papain-agarose. L-cystatin inhibits amidol...

journal_title：Journal of biochemistry

authors： Agarwala KL,Kawabata S,Hirata M,Miyagi M,Tsunasawa S,Iwanaga S

更新日期：1996-01-01 00:00:00

abstract：:1. Porcine cardiac native tropomyosin was phosphorylated by bovine cardiac 3':5'-cyclic AMP-dependent protein kinase. Most of the phosphate incorporation was observed in troponin I, the maximum of which was 0.7 mol of Pi per mol of troponin I. 2. In the presence of phosphorylated native tropomyosin, actomyosin ATPase ...

journal_title：Journal of biochemistry

authors： Yamamoto K,Ohtsuki I

更新日期：1982-05-01 00:00:00

abstract：:The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

journal_title：Journal of biochemistry

authors： Inoue H,Takahashi S,Fukui K,Miyake Y

更新日期：1991-10-01 00:00:00

abstract：:Human neutrophil elastase was purified to homogeneity as two isozymes named E1 and E2. The isozymes degraded Type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan similarly to each other. The degradation of such basement membrane components by elastase may assist the extravasation of neutrophils in ...

journal_title：Journal of biochemistry

authors： Watanabe H,Hattori S,Katsuda S,Nakanishi I,Nagai Y

更新日期：1990-11-01 00:00:00

abstract：:Oligosaccharides of a non-oligomannoside type were released from porcine alpha-mannosidase by hydrazinolysis, and were fractionated into at least 15 homogeneous oligosaccharides. Most of them are oligosaccharides with galactose and N-acetylglucosamine residues attached to a common core, alpha Man2 beta Man beta GlcNAc...

journal_title：Journal of biochemistry

authors： Kozutsumi Y,Kawasaki T,Yamashina I

更新日期：1986-08-01 00:00:00