The Edg family G protein-coupled receptors for lysophospholipids: their signaling properties and biological activities.


:Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated platelets appear to be a major source of both S1P and LPA in blood. Despite the diversity of their biosynthetic origins, they are considered to share substantial structural similarity. Indeed, recent investigation has revealed that S1P and LPA act via a single family of G protein-coupled receptors designated as Edg. Thus, the Edg isoforms, Edg1 (also called S1P(1)), Edg5 (S1P(2)), Edg3 (S1P(3)), Edg6 (S1P(4)), and Edg8 (S1P(5)), are specific receptors for S1P (and SPC with a lower affinity), whereas Edg2 (LPA(1)), Edg4 (LPA(2)), and Edg7 (LPA(3)) serve as receptors specific for LPA. Each receptor isoform displays a unique tissue expression pattern and coupling to a distinct set of heterotrimeric G proteins, leading to the activation of an isoform-specific panel of multiple intracellular signaling pathways. Recent studies on knockout mice have unveiled non-redundant Edg receptor functions that are essential for normal development and vascular maturation. In addition, the Edg lysophospholipid signaling system may play a role in modulating cell motility under such pathological conditions as inflammation, tumor cell dissemination and vascular remodeling.


J Biochem


Journal of biochemistry


Takuwa Y,Takuwa N,Sugimoto N





Has Abstract


2002-06-01 00:00:00












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    abstract::Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Teshima K,Ikeda K,Hamaguchi K,Hayashi K

    更新日期:1983-07-01 00:00:00

  • Consequential alterations in haemoglobin structure upon glycation with fructose: prevention by acetylsalicylic acid.

    abstract::Increased fructose concentration in erythrocytes of diabetic patients subject haemoglobin (Hb) to be glycated by fructose. Haemoglobin glycation results in early and advanced glycation end products which are known as HbA(1c) and Hb-AGE, respectively. In diabetics the Hb-AGE content raises and the mean parameter multip...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Bakhti M,Habibi-Rezaei M,Moosavi-Movahedi AA,Khazaei MR

    更新日期:2007-06-01 00:00:00

  • Prolyl aminopeptidase from Serratia marcescens: cloning of the enzyme gene and crystallization of the expressed enzyme.

    abstract::We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kabashima T,Kitazono A,Kitano A,Ito K,Yoshimoto T

    更新日期:1997-09-01 00:00:00

  • Histidine cycle mechanism for the concerted proton/electron transfer from ascorbate to the cytosolic haem b centre of cytochrome b561: a unique machinery for the biological transmembrane electron transfer.

    abstract::Cytochromes b(561) are a family of transmembrane proteins found in most eukaryotic cells and contain two haem b prosthetic groups per molecule being coordinated with four His residues from four different transmembrane alpha-helices. Although cytochromes b(561) residing in the chromaffin vesicles has long been known to...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakanishi N,Takeuchi F,Tsubaki M

    更新日期:2007-11-01 00:00:00

  • Studies on stringent control in a cell-free system. Regulation by guanosine-5'-diphosphate-3'-diphosphate of the synthesis of elongation factor Tu.

    abstract::The biosynthesis of elongation factor Tu (EF-Tu) has been studied in a cell-free system with DNA of the transducing phage lambdarifd18 as a template. It was found that the synthesis of EF-Tu in this system was inhibited by about 60% in the presence of 0.3 to 0.6 mM guanosine-5'-diphosphate-3'-diphosphate (ppGpp). The ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shibuya M,Kaziro Y

    更新日期:1979-08-01 00:00:00

  • Disulfide bond interchange in Escherichia coli-derived recombinant human interferon-beta 1 under denaturing conditions.

    abstract::Disulfide bond interchange has been pointed out as a considerable problem in preparing recombinant proteins from Escherichia coli cells. This has been reported in the system of reducing denaturation followed by a refolding process, where incorrectly folded molecules are sometimes produced. As the possibility of disulf...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kimura S,Utsumi J,Yamazaki S,Shimizu H

    更新日期:1988-07-01 00:00:00

  • Studies on nitrate reductase of Clostridium perfringens. Purification, some properties, and effect of tungstate on its formation.

    abstract::Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Seki-Chiba S,Ishimoto M

    更新日期:1977-12-01 00:00:00

  • Detection of novel metabolite for Roxadustat doping by global metabolomics.

    abstract::Roxadustat (FG-4592, Rox) is a stabilizer for hypoxia-inducible transcription factors (HIFs), which induce production of the erythroid growth factor erythropoietin, and has been listed by the World Anti-Doping Agency as a prohibited substance for athletes since 2011. Although the detection technologies for Rox and its...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saigusa D,Suzuki N,Matsumoto Y,Umeda K,Tomioka Y,Koshiba S,Yamamoto M

    更新日期:2018-02-09 00:00:00

  • Identification of the catalytic residues of sequence-specific and histidine-free ribonuclease colicin E5.

    abstract::Colicin E5 cleaves tRNAs for Tyr, His, Asn and Asp in their anticodons to abolish protein synthesis in Escherichia coli. We previously showed how its C-terminal RNase domain, E5-CRD, recognizes the anticodon bases but the catalytic mechanism remained to be elucidated. Although the reaction products with 5'-OH and 2',3...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inoue-Ito S,Yajima S,Fushinobu S,Nakamura S,Ogawa T,Hidaka M,Masaki H

    更新日期:2012-10-01 00:00:00

  • Proton-relay system of carboxypeptidase Y as a sole catalytic site: studies on mutagenic replacement of his 397.

    abstract::His397 was replaced with alanine by site-directed mutagenesis of the cloned PRC1 gene in order to confirm the role of this residue in the proton-relay system of carboxypeptidase Y (CPY). The expressed and purified H397A showed a CD spectrum almost identical to that of the wild type enzyme, but its heat stability and c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Jung G,Ueno H,Hayashi R

    更新日期:1998-08-01 00:00:00

  • Multiple forms of ribonuclease H from rat liver cytosol.

    abstract::Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sawai Y,Yanokura M,Tsukada K

    更新日期:1979-09-01 00:00:00

  • Ionic strength and pH effects on the rates of reduction of spinach plastocyanin by ascorbate.

    abstract::Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-H...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takabe T,Niwa S,Ishikawa H,Miyakawa M

    更新日期:1980-01-01 00:00:00

  • A splicing factor, Prp8: preferential localization in the testis and ovary in adult mice.

    abstract::Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

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    pub_type: 杂志文章


    authors: Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

    更新日期:2001-04-01 00:00:00

  • Cloning and characterization of ferredoxin and ferredoxin-NADP+ reductase from human malaria parasite.

    abstract::The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned and studied the biochemical properties of plant-type ferredoxin (Fd) and Fd-NADP+ reductase (FNR), a redox system that p...

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    pub_type: 杂志文章


    authors: Kimata-Ariga Y,Kurisu G,Kusunoki M,Aoki S,Sato D,Kobayashi T,Kita K,Horii T,Hase T

    更新日期:2007-03-01 00:00:00

  • Chemical replacement of P1' serine residue at the second reactive site of soybean protease inhibitor C-II.

    abstract::The P1' Ser(50) at the second reactive site of soybean protease inhibitor C-II was replaced with arginine to confirm the contribution of this residue to the inhibition. The Arg derivative had less trypsin inhibitory activity (Ki = 1 X 10(-7) M) than the Ser derivative (Ki = 2 X 10(-8) M), in contrast to the results ob...

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    pub_type: 杂志文章


    authors: Kurokawa T,Hara S,Teshima T,Ikenaka T

    更新日期:1987-09-01 00:00:00

  • Studies on D-tetrose metabolism. VI. Crystallization and some properties of D-erythrulose reducatase from beef liver.

    abstract::D-Erythrulose reductase of beef liver was crystallized from ammonium sulfate solution at pH 8.17. The crystals are needle-shaped. The enzyme protein contains 851 amino acid residues per mole of the enzyme: Lys28, His11, Arg52, Asp79, Thr58, Ser56, Glu68, Pro20, Gly80, Ala107, Val112, Met24, Ile31, Leu88, Tyr7, Phe22, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Uehara K,Tanimoto T

    更新日期:1975-09-01 00:00:00

  • Structural study of the N-linked oligosaccharides of hepatocyte growth factor by two-dimensional sugar mapping.

    abstract::The structures of the N-linked oligosaccharides on recombinant human hepatocyte growth factor (rh-HGF) expressed by Chinese hamster ovary (CHO) cells were studied by two-dimensional sugar mapping. The oligosaccharides released from the glycopeptides by peptide: N-glycosidase F (PNGase F) treatment were tagged with 2-a...

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    pub_type: 杂志文章


    authors: Hara H,Nakae Y,Sogabe T,Ihara I,Ueno S,Sakai H,Inoue H,Shimizu S,Nakamura T,Shimizu N

    更新日期:1993-07-01 00:00:00

  • Immunological neutralization of cobrotoxin by its homologous precipitin and non-precipitin antibodies.

    abstract::Anti-cobrotoxin antibodies can be separated into precipitin and non-precipitin antibodies. The precipitin antibody possesses the same binding affinity to cobrotoxin as non-precipitin antibody, but the neutralizing capability of the latter is superior to that of the former in blocking cobrotoxin binding to nicotinic ac...

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    pub_type: 杂志文章


    authors: Kuo KW,Chang LS,Lin PM,Leaber RJ,Chang CC

    更新日期:1994-12-01 00:00:00

  • Translation efficiency affects the sequence-independent +1 ribosomal frameshifting by polyamines.

    abstract::Antizyme (AZ) interacts with ornithine decarboxylase, which catalyzes the first step of polyamine biosynthesis and recruits it to the proteasome for degradation. Synthesizing the functional AZ protein requires transition of the reading frame at the termination codon. This programmed +1 ribosomal frameshifting is induc...

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    pub_type: 杂志文章


    authors: Oguro A,Shigeta T,Machida K,Suzuki T,Iwamoto T,Matsufuji S,Imataka H

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  • Effect of non-native helix destabilization on the folding of equine β-lactoglobulin.

    abstract::β-lactoglobulin forms a non-native α-helix during an early stage of folding. To address the role of the non-native structure in the folding process, we designed several mutants of equine β-lactoglobulin with reduced helical propensity in the non-native helix region. One of them, A123T, showed a similar structure to th...

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    pub_type: 杂志文章


    authors: Okabe T,Miyajima T,Nakagawa K,Tsukamoto S,Fujiwara K,Ikeguchi M

    更新日期:2014-11-01 00:00:00

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    abstract::The activity of cathepsin E (M(r) approximately 80K), a dimeric aspartic proteinase with two active sites per molecule, toward a protein substrate (reduced and carboxymethylated ribonuclease A) was shown to be completely inhibited by alpha 2-macroglobulin (alpha 2M) at pH 5.5. On the other hand, the activity toward a ...

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    pub_type: 杂志文章


    authors: Athauda SB,Arakawa H,Nishigai M,Takahashi T,Ikai A,Takahashi K

    更新日期:1993-05-01 00:00:00

  • Elucidation of the phenotypic change on the surface of Had-1 cell, a mutant cell line of mouse FM3A carcinoma cells selected by resistance to Newcastle disease virus infection.

    abstract::Had-1, which was isolated from mouse FM3A carcinoma cells, was a non-permissive mutant cell line to Newcastle disease virus infection. Comparative study of the asparagine-linked sugar chains of the surface glycoproteins of the mutant and its parental cells revealed that galactosylation of the complex-type sugar chains...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hara T,Endo T,Furukawa K,Kawakita M,Kobata A

    更新日期:1989-08-01 00:00:00

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    abstract::Interactions between phospholipid membranes and the acyl chain and specific amino acid residues of myristoylated proteins are necessary for membrane association. In the present study we tested the effects of mutations of calcineurin B subunit amino acid residues K(20)K(21), K(24)R(25), K(27)K(28) to Glu on the interac...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Martin BA,Oxhorn BC,Rossow CR,Perrino BA

    更新日期:2001-05-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Itoh H,Tomita M,Kobayashi T,Uchino H,Maruyama H,Nawa Y

    更新日期:1996-08-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Saito M,Toyoshima S,Osawa T

    更新日期:1977-05-01 00:00:00

  • Characterization of the diacylglycerol acyltransferase activity in the lipid body fraction from an oleaginous fungus.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kamisaka Y,Nakahara T

    更新日期:1994-12-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hamano M,Nitta K,Kuwajima K,Sugai S

    更新日期:1986-12-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kitahata S,Okada S

    更新日期:1976-03-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsuda S

    更新日期:1988-12-01 00:00:00

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Watanabe A,Yonemura I,Gonda K,Numata O

    更新日期:2000-01-01 00:00:00