Abstract:
:In order to characterize the mechanism for bilirubin transport in the liver, the uptake of bilirubin diglucuronide (BDG) into purified sinusoidal plasma membrane vesicles was investigated. BDG uptake was saturable, and was inhibited by sulfobromophthalein and unconjugated bilirubin, but was not affected by sodium taurocholate. BDG uptake was sodium-independent and was stimulated by intravesicular bilirubin or BDG (trans-stimulation). BDG transport showed strong potential sensitivity; vesicle inside-negative membrane potential created by different anion gradients inhibited BDG uptake whereas vesicle inside-positive membrane potential generated by potassium gradients and valinomycin markedly stimulated BDG transport. These data suggest that BDG, sulfobromophthalein, and probably unconjugated bilirubin share a common transporter in liver cells which is sodium independent, membrane-potential-dependent and capable of exchange. The direction of transport in vivo may be governed by the intracellular concentration of BDG and of other yet unidentified organic anions sharing this transporter.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Adachi Y,Roy-Chowdhury J,Roy-Chowdhury N,Kinne R,Tran T,Kobayashi H,Arias IMdoi
10.1093/oxfordjournals.jbchem.a123120subject
Has Abstractpub_date
1990-05-01 00:00:00pages
749-54issue
5eissn
0021-924Xissn
1756-2651journal_volume
107pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1975-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1992-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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更新日期:1984-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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更新日期:1977-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1980-09-01 00:00:00
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