Intradermal catabolism of rabbit IgG and its fragments.

abstract：:Oligosaccharides of a non-oligomannoside type were released from porcine alpha-mannosidase by hydrazinolysis, and were fractionated into at least 15 homogeneous oligosaccharides. Most of them are oligosaccharides with galactose and N-acetylglucosamine residues attached to a common core, alpha Man2 beta Man beta GlcNAc...

journal_title：Journal of biochemistry

authors： Kozutsumi Y,Kawasaki T,Yamashina I

更新日期：1986-08-01 00:00:00

abstract：:Pure bovine rhodopsin pellets were prepared by removal of cholic acid from rhodopsin-cholic acid complex, and X-ray diffraction patterns from the internal structure of rhodopsin were obtained for the first time using wet and dry pellet samples. The peaks at around 10 and 4.3 A spacings observed for both samples can be...

journal_title：Journal of biochemistry

authors： Kawaguchi T,Hamanaka T,Kito Y

更新日期：1996-03-01 00:00:00

abstract：:The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin...

journal_title：Journal of biochemistry

authors： Srimal S,Miyata T,Kawabata S,Miyata T,Iwanaga S

更新日期：1985-08-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:ENO1-'lacZ fusions with various lengths of the ENO1 5'-flanking region were constructed on various types of yeast plasmid vectors. The fully expressed level of beta Gal directed by ENO1-'lacZ fusions differed depending on the type of vector, but on any type of vector, beta Gal activity was not greatly influenced by th...

journal_title：Journal of biochemistry

authors： Uemura H,Shiba T,Machida M,Matsui I,Jigami Y,Tanaka H

更新日期：1987-07-01 00:00:00

abstract：:In sea urchin embryos, primary mesenchyme cells, descendants from micromeres produced at the 16-cell stage, form spicules or CaCO3 deposits in their skeletal vacuoles, at the post-gastrula stage. Micromeres isolated at the 16-cell stage also differentiate into spicule-forming cells during their culture at the same tim...

journal_title：Journal of biochemistry

authors： Mitsunaga K,Fujino Y,Yasumasu I

更新日期：1986-12-01 00:00:00

abstract：:Human serum sulfotransferase activities were measured in normal controls and patients with macular corneal dystrophy (MCD), an inherited disorder characterized by the decreased sulfation of keratan sulfate in the corneal stroma and serum, using two kinds of acceptor: partially desulfated keratan sulfate and a trisacch...

journal_title：Journal of biochemistry

authors： Hasegawa N,Torii T,Nagaoka I,Nakayasu K,Miyajima H,Habuchi O

更新日期：1999-02-01 00:00:00

abstract：:S-RNase is associated with the gametophytic self-incompatibility of flowering plants in Solanaceae and, on the basis of sequence homology, belongs to the RNase T2 family. To identify the active site residues in S-RNase, Nicotiana alata S6-RNase was studied by chemical modification. S6-RNase was inactivated with iodoac...

journal_title：Journal of biochemistry

authors： Ishimizu T,Miyagi M,Norioka S,Liu YH,Clarke AE,Sakiyama F

更新日期：1995-11-01 00:00:00

abstract：:We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-tre...

journal_title：Journal of biochemistry

authors： Hoshi K,Kariya Y,Nara K,Ito H,Matsumoto K,Nagae M,Yamaguchi Y,Nakajima M,Miyajima M,Arai H,Kuno A,Narimatsu H,Shirotani K,Hashimoto Y

更新日期：2013-09-01 00:00:00

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a ligand of EGF receptor, is involved in the growth and malignant progression of cancers. Cross-reacting material 197, CRM197, a non-toxic mutant of diphtheria toxin (DT), specifically binds to the EGF-like domain of HB-EGF and inhibits its mitogenic...

journal_title：Journal of biochemistry

authors： Suzuki K,Mizushima H,Abe H,Iwamoto R,Nakamura H,Mekada E

更新日期：2015-05-01 00:00:00

abstract：:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we c...

journal_title：Journal of biochemistry

authors： Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang M

更新日期：2019-09-01 00:00:00

abstract：:YfkN isolated from the culture supernatant of Bacillus subtilis in the exponential phase of growth is a protein of 143.5 kDa that derives from a putative large precursor of 159.6 kDa processed at both the N- and C-terminal ends. Pulse-chase experiments indicated that the release occurs slowly with a half-time longer t...

journal_title：Journal of biochemistry

authors： Chambert R,Pereira Y,Petit-Glatron MF

更新日期：2003-11-01 00:00:00

abstract：:G-actin freed from exogenous ATP was added to the pieces of isolated acrosomal actin bundles from horseshoe crab sperm to form filaments as reported earlier (Tilney, L.G., Bonder, E.M., & DeRosier, D.J. (1981) J. Cell Biol. 90, 485-494). The growth of a filament was far more rapid at one end (the preferred end) than t...

journal_title：Journal of biochemistry

authors： Mabuchi I

更新日期：1983-10-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:A new pathway of NAD+ synthesis from anthranilic acid was found in the livers of rats. Starting from [carboxyl-14C]anthranilic acid, radioactive NAD+ and NADP+ were produced as judged by Dowex-1 X 8-formate column chromatography followed by radiochromatography. Several intermediate compounds, such as quinolinic acid, ...

journal_title：Journal of biochemistry

authors： Ueda T,Otsuka H,Goda K,Ishiguro I,Naito J,Kotake Y

更新日期：1978-09-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:Archaeal geranylgeranyl diphosphate (GGPP) synthase catalyzes the consecutive condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to produce GGPP with significant amounts of intermediates. To obtain information about the amino acids involved in the condensation and the release of intermediates, we ...

journal_title：Journal of biochemistry

authors： Ohnuma S,Hemmi H,Ohto C,Nakane H,Nishino T

更新日期：1997-04-01 00:00:00

abstract：:Recombinant horse L-apoferritin and its mutants were used to compare the reactivities of two different cysteinyl residues with 7-fluoro-4-sulfamoyl-2,1,3-benzoxadiazole (ABD-F), p-chloromercuribenzoic acid (PCMB), N-(9-acridinyl)maleimide (NAM), and 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl (NEM-TEMPO). ABD-F s...

journal_title：Journal of biochemistry

authors： Takeda S,Yamaki M,Ebina S,Nagayama K

更新日期：1995-02-01 00:00:00

abstract：:We synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. We solubilized membrane proteins of Vibrio parahaemolyticus using the detergents. With octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfacto...

journal_title：Journal of biochemistry

authors： Izawa S,Sakai-Tomita Y,Kinomura K,Kitazawa S,Tsuda M,Tsuchiya T

更新日期：1993-05-01 00:00:00

abstract：:Midkine (MK) is a product of a retinoic acid-responsive gene and a new heparin-binding growth/differentiation factor. The coding sequence of human MK was located on 1.5 kb DNA segment. The structure of the cloned human MK gene was determined and compared with that of the mouse gene. The coding sequence was divided int...

journal_title：Journal of biochemistry

authors： Uehara K,Matsubara S,Kadomatsu K,Tsutsui J,Muramatsu T

更新日期：1992-05-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:1. From the standpoint of monomer-dimer equilibrium of hog kidney D-amino acid oxidase [EC 1.4.3.3] and the interaction between the enzyme and small molecules, the effect of pH on the binding of p-aminobenzoate to the monomer and dimer of the enzyme was studied by kinetic methods and spectrophotometric titration. 2. T...

journal_title：Journal of biochemistry

authors： Horiike K,Shiga K,Isomoto A,Yamano T

更新日期：1976-11-01 00:00:00

abstract：:The oxygen exchange during ATP hydrolysis by glycerinated muscle fibers, myofibrils, and synthetic actomyosin filaments was studied from the distribution of the [18O]Pi species produced by the hydrolysis of [gamma-18O]ATP. The products were mixtures of two species, one with a low extent of oxygen exchange and the othe...

journal_title：Journal of biochemistry

authors： Yasui M,Ohe M,Kajita A,Arata T,Inoue A

更新日期：1989-04-01 00:00:00

abstract：:Accumulation of pyrophosphate induced by acetate administration was investigated in rat liver in situ and in perfused rat liver. Intraperitoneal injection of acetate into rats increased the pyrophosphate concentration in the liver to about 2 mumol/g liver, which was 200 times that in control liver. Perfusion of liver ...

journal_title：Journal of biochemistry

authors： Yamada T,Inoue T,Nishida T,Furuya E,Tagawa K

更新日期：1988-11-01 00:00:00

abstract：:Ribose-modified fluorescent nucleotide analogs, 3'-O-anthraniloyl and 3'-O-(N-methylanthraniloyl) derivatives of AT(D)P, dAT(D)P, CT(D)P, UT(D)P, IT(D)P, and GT(D)P, were synthesized for use as substrates and affinity labels for the myosin ATPase [Hiratsuka, T. (1983) Biochim. Biophys. Acta 742, 496-508]. None of the ...

journal_title：Journal of biochemistry

authors： Hiratsuka T

更新日期：1984-07-01 00:00:00

abstract：:Manganese ion, like Mg2+, has been found to produce high biosynthetic activity of the unadenylylated form of glutamine synthetase obtained from Mycobacterium smegmatis, and the activity with each of these cations was decreased by the adenylylation of the enzyme. Further, the gamma-glutamyltransferase reaction was cata...

journal_title：Journal of biochemistry

authors： Matsuoka K,Kimura K

更新日期：1985-04-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka S

更新日期：1989-05-01 00:00:00

abstract：:The inhibitory effects of galloyl pedunculagin (GP) and eugeniin on the phosphorylation of histone H2B by cAMP-dependent protein kinase (A-kinase) and autophosphorylation of its beta-regulatory subunit (A-kinase beta) were examined in vitro. It was found that (i) GP (ID(50) = approx. 50 nM) effectively inhibits the ac...

journal_title：Journal of biochemistry

authors： Kosuge S,Maekawa T,Saito C,Tanaka T,Kouno I,Ohtsuki K

更新日期：2001-03-01 00:00:00