Affinity labeling of the myosin ATPase with ribose-modified fluorescent nucleotides and vanadate.

Abstract:

:Ribose-modified fluorescent nucleotide analogs, 3'-O-anthraniloyl and 3'-O-(N-methylanthraniloyl) derivatives of AT(D)P, dAT(D)P, CT(D)P, UT(D)P, IT(D)P, and GT(D)P, were synthesized for use as substrates and affinity labels for the myosin ATPase [Hiratsuka, T. (1983) Biochim. Biophys. Acta 742, 496-508]. None of the fluorescent nucleoside triphosphate (NTP) analogs was significantly different from the corresponding natural NTP in its ability to support superprecipitation of actomyosin. When fluorescent and natural NTPs were used as substrates for the myosin subfragment-1(S-1) ATPase in the presence of 1mM vanadate ion (V1), a slight initial inhibition of the S-1 NTPase was followed by progressive inhibition to more than 60% over a period of 1 h. The apparent second-order rate constants were 0.14-0.44M-1 . s-1, suggesting the formation of the inactive fluorescent NDP-labeled S-1. After incubation of S-1 with the nucleoside diphosphate (NDP) analog in the presence of Vi, the resultant fluorescent NDP-labeled S-1 was isolated free of unbound Vi and the analog by gel filtration. The isolated complexes had stoichiometries of 0.6-1.1 NDP analog per S-1 active site. Native polyacrylamide gel electrophoresis revealed conveniently that the NDP analog is associated with S-1 as indicated by two intense fluorescent bands corresponding to S-1 isozymes. On dissociating gels, the analog was released from S-1, suggesting that the labeled S-1 is held together by strong secondary forces rather than covalent bonds.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

J Biochem

journal_title

Journal of biochemistry

authors

Hiratsuka T

doi

10.1093/oxfordjournals.jbchem.a134806

subject

Has Abstract

pub_date

1984-07-01 00:00:00

pages

147-54

issue

1

eissn

0021-924X

issn

1756-2651

journal_volume

96

pub_type

杂志文章
  • The Edg family G protein-coupled receptors for lysophospholipids: their signaling properties and biological activities.

    abstract::Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated plate...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审

    doi:10.1093/oxfordjournals.jbchem.a003163

    authors: Takuwa Y,Takuwa N,Sugimoto N

    更新日期:2002-06-01 00:00:00

  • Polyamine-dependent deoxyribonuclease activity from rat-liver nuclei.

    abstract::When nuclei isolated from rat liver in a low salt buffer were washed with 0.1 M NaCl solution, the supernatant showed a deoxyribonuclease (DNase) activity. The activity required Mg2+ and in addition spermine or spermidine, and its optimal pH was 7.2-7.4. The activity was higher on denatured (single stranded) DNA than ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a131417

    authors: Nakano T,Tsuboi M

    更新日期:1976-12-01 00:00:00

  • Detection of DNA replicase activity in rat thymoma cells.

    abstract::A DNA polymerase which was active with unprimed poly(dC) as a template was detected in the DNA polymerase alpha fraction from rat thymoma tissue. The activity was dependent on rGTP and associated with ribonucleotide polymerizing activity. The activity was partially sensitive to aphidicolin and dideoxy GTP and resistan...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a134165

    authors: Nishizawa M,Tanabe K,Matsukage A,Nakamura H,Yagura T

    更新日期:1983-01-01 00:00:00

  • Proteasome inhibitors block Ras/ERK signaling pathway resulting in the downregulation of Fas ligand expression during activation-induced cell death in T cells.

    abstract::Activation-induced cell death (AICD) plays a critical role in the maintenance of homeostasis and peripheral tolerance in the immune system, and is mediated by Fas ligand (FasL) expression and the interaction between Fas and FasL. In the present study, we examined the role of the ubiquitin-proteasome system in AICD usi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a003106

    authors: Tanimoto Y,Kizaki H

    更新日期:2002-03-01 00:00:00

  • Semi-micro-scale frontal gel chromatography of interacting systems of a protein and small molecules: binding of warfarin, tryptophan, or FMN to albumin, and of o-nitrophenol to catechol 2,3-dioxygenase.

    abstract::Frontal gel chromatography is a convenient and accurate method to obtain the free ligand concentration of a protein-ligand mixture. Because a large amount of sample (more than 6 ml) is required for the method, it has been rarely used for binding experiments. We have developed a system to carry out frontal gel chromato...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021747

    authors: Honjo M,Ishida T,Horiike K

    更新日期:1997-08-01 00:00:00

  • State of association of band 3 protein from bovine erythrocyte membrane in nonionic detergent.

    abstract::The state of association of bovine Band 3, which is a major intrinsic protein of erythrocyte membranes, was examined in nonaethyleneglycol dodecyl ether (C12E9) solution by ultracentrifugation, gel filtration, gel electrophoresis and cross-linking studies. The molecular weight of bovine Band 3 was 107,000 +/- 5% as de...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133081

    authors: Nakashima H,Makino S

    更新日期:1980-10-01 00:00:00

  • The structural and functional organization of intramolecular chaperones: the N-terminal propeptides which mediate protein folding.

    abstract::A large number of prokaryotic as well as eukaryotic proteins are produced with amino terminal propeptides. These amino-terminal extensions are essential for mediating proper folding of their corresponding proteins and are also termed as intramolecular chaperones. Though these propeptides are highly specific and unique...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审

    doi:10.1093/oxfordjournals.jbchem.a124386

    authors: Shinde U,Inouye M

    更新日期:1994-04-01 00:00:00

  • Characterization of a human placental fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase.

    abstract::A full-length cDNA, which encodes a human placental fructose-6-phosphate,2-kinase/ fructose-2,6-bisphosphatase, was constructed and expressed in Escherichia coli. The expressed protein, purified to homogeneity, showed a molecular weight of 58,000 by gel electrophoresis under denaturing conditions, compared to the dedu...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021719

    authors: Sakakibara R,Kato M,Okamura N,Nakagawa T,Komada Y,Tominaga N,Shimojo M,Fukasawa M

    更新日期:1997-07-01 00:00:00

  • Vasoactive intestinal peptide induces differentiation and MAP kinase activation in PC12h cells.

    abstract::Vasoactive intestinal peptide (VIP), a neuropeptide coupled with adenylate cyclase, was found to induce neurite extension of PC12h cells. Neurites appeared within 1 h after addition of VIP and extended for at least 24 h. The half-maximal concentration for the effect of VIP was 50 nM. In addition to the morphological c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124333

    authors: Okumura N,Miyatake Y,Takao T,Tamaru T,Nagai K,Okada M,Nakagawa H

    更新日期:1994-02-01 00:00:00

  • Kinetic studies on the cleavage of oligouridylic acids and poly U by bovine pancreatic ribonuclease A.

    abstract::Kinetic parameters, Km and Vmax for the transesterification of oligouridylic acid, (Up)nU greater than p (n=0-4), by RNase A were measured spectrophotometrically at pH 7.0 and 25 degrees C. The kinetic parameters, pKm and log Vmax increased with increase in the chain length (n), and seemed to be almost constant with s...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a134833

    authors: Irie M,Mikami F,Monma K,Ohgi K,Watanabe H,Yamaguchi R,Nagase H

    更新日期:1984-07-01 00:00:00

  • A new regulatory protein that affects the state of actin polymerization.

    abstract::A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state vi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133363

    authors: Nishida E,Kuwaki T,Maekawa S,Sakai H

    更新日期:1981-05-01 00:00:00

  • X-ray diffraction pattern from internal structure of bovine rhodopsin.

    abstract::Pure bovine rhodopsin pellets were prepared by removal of cholic acid from rhodopsin-cholic acid complex, and X-ray diffraction patterns from the internal structure of rhodopsin were obtained for the first time using wet and dry pellet samples. The peaks at around 10 and 4.3 A spacings observed for both samples can be...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a021254

    authors: Kawaguchi T,Hamanaka T,Kito Y

    更新日期:1996-03-01 00:00:00

  • Fields, biochemistry fast autooxidation of a Bis-Histidyl-ligated globin from the anhydrobiotic tardigrade, ramazzottius varieornatus, by molecular oxygen.

    abstract::Tardigrades, a phylum of meiofaunal organisms, exhibit extraordinary tolerance to various environmental conditions, including extreme temperatures (-272 to 151 °C) and exposure to ionizing radiation. Proteins from anhydrobiotic tardigrades with homology to known proteins from other organisms are new potential targets ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvab003

    authors: Kobayashi K,Kim J,Fukuda Y,Kozawa T,Inoue T

    更新日期:2021-01-21 00:00:00

  • Studies on stringent control in a cell-free system. Regulation by guanosine-5'-diphosphate-3'-diphosphate of the synthesis of elongation factor Tu.

    abstract::The biosynthesis of elongation factor Tu (EF-Tu) has been studied in a cell-free system with DNA of the transducing phage lambdarifd18 as a template. It was found that the synthesis of EF-Tu in this system was inhibited by about 60% in the presence of 0.3 to 0.6 mM guanosine-5'-diphosphate-3'-diphosphate (ppGpp). The ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a132539

    authors: Shibuya M,Kaziro Y

    更新日期:1979-08-01 00:00:00

  • Steady-state properties of calcium binding to parvalbumins from bullfrog skeletal muscle: effects of Mg2+, pH, ionic strength, and temperature.

    abstract::To improve our understanding of the physiological roles of parvalbumins, PA-1 (pI 4.78) and PA-2 (pI 4.97) parvalbumins were prepared from bullfrog skeletal muscle and their calcium binding properties were examined in a medium of constant ionic strength (I = 0.106, pH 6.80, at 20 degrees C) containing various concentr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a135481

    authors: Ogawa Y,Tanokura M

    更新日期:1986-01-01 00:00:00

  • Catalytic properties of NADPH-specific dihydropteridine reductase from bovine liver.

    abstract::The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a135523

    authors: Nakanishi N,Hasegawa H,Akino M,Yamada S

    更新日期:1986-03-01 00:00:00

  • Isolation and amino acid sequence of a phospholipase A2 inhibitor from the blood plasma of Agkistrodon blomhoffii siniticus.

    abstract::Phospholipase A2 inhibitor (PLI) was purified from the blood plasma of Chinese Mamushi, Agkistrodon blomhoffii siniticus, by sequential chromatography on Sephadex G-200, Mono Q, and Blue-Sepharose CL-6B columns. The purified PLI was a glycoprotein with an apparent molecular mass of 75 kDa and was composed of a single ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124060

    authors: Ohkura N,Inoue S,Ikeda K,Hayashi K

    更新日期:1993-04-01 00:00:00

  • Inhibition of eukaryotic DNA polymerase-alpha by polydeoxynucleotides.

    abstract::Effects of polydeoxynucleotides on the activity of DNA polymerase-alpha from sea urchin embryos were studied. Poly(dG), poly(dC) and poly(dC)-oligo(dG)12-18 inhibited DNA polymerase-alpha activity in the activated DNA-directed reaction but poly(dA), oligo(dT)12-18, and poly(dA)-oligo(dT)12-18 did not inhibit the activ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133969

    authors: Oguro M,Nagano H

    更新日期:1982-08-01 00:00:00

  • Redox properties of membrane-bound b-type cytochromes and a soluble c-type cytochrome of nitrate reductase in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans.

    abstract::In order to identify the b-type cytochrome involved in the nitrate reduction in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans, the b-type cytochromes in the spheroplast membranes were characterized. Difference spectra at 77K of spheroplast membranes indicated the presence of two b-type cytoc...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a134765

    authors: Yokota S,Urata K,Satoh T

    更新日期:1984-06-01 00:00:00

  • Structures of replacement vectors for efficient gene targeting.

    abstract::The relationship between DNA structure of replacement vectors and gene targeting efficiency was studied using positive-negative selection. The vectors contained pBR322 DNA, a bacterial neomycin-resistance gene (neo) for positive selection, a herpes simplex virus (HSV) thymidine kinase gene (tk) for negative selection,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124362

    authors: Horie K,Nishiguchi S,Maeda S,Shimada K

    更新日期:1994-03-01 00:00:00

  • Identification of beta1,4GalT II as a target gene of p53-mediated HeLa cell apoptosis.

    abstract::beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regula...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvn003

    authors: Zhou J,Wei Y,Liu D,Ge X,Zhou F,Jiang XY,Gu J

    更新日期:2008-04-01 00:00:00

  • Human aldolase C: characterization of the recombinant enzyme expressed in Escherichia coli.

    abstract::To study the structure/function relationship and enzymatic properties of human aldolase C, we have constructed an Escherichia coli expression plasmid, pHAC11, for the isozyme. E. coli cells carrying this plasmid produced enzymatically active human aldolase C. The kcat and Km values for fructose-1,6-bisphosphate (Fru-1...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a124475

    authors: Kusakabe T,Motoki K,Hori K

    更新日期:1994-06-01 00:00:00

  • Two distinct ferredoxins from Rhodobacter capsulatus: complete amino acid sequences and molecular evolution.

    abstract::Two distinct ferredoxins were purified from Rhodobacter capsulatus SB1003. Their complete amino acid sequences were determined by a combination of protease digestion, BrCN cleavage and Edman degradation. Ferredoxins I and II were composed of 64 and 111 amino acids, respectively, with molecular weights of 6,728 and 12,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a123224

    authors: Saeki K,Suetsugu Y,Yao Y,Horio T,Marrs BL,Matsubara H

    更新日期:1990-09-01 00:00:00

  • Binding of intrinsic ATPase inhibitor to mitochondrial ATPase--stoichiometry of binding of nucleotides, inhibitor, and enzyme.

    abstract::F1-ATPase inhibitor was purified from yeast, Saccharomyces cerevisiae. The purified inhibitor blocked ATPase activity in the presence of ATP and Mg2+ by forming a latent equimolar enzyme-inhibitor complex with ATP and ADP newly bound to loose sites on the enzyme. A small portion of externally added ATP was hydrolyzed ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133567

    authors: Hashimoto T,Negawa Y,Tagawa K

    更新日期:1981-10-01 00:00:00

  • Splice-site signals of mRNA precursors as revealed by computer search. Site-specific mutagenesis and thalassemia.

    abstract::Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, applying the computer searching method to primary ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a135162

    authors: Iida Y

    更新日期:1985-04-01 00:00:00

  • The teratogenic mechanism of 6-aminonicotinamide on limb formation of chick embryos: abnormalities in the biosynthesis of glycosaminoglycans and proteoglycans in micromelia.

    abstract::After a dose of 10 micrograms of 6-aminonicotinamide (6-AN) was administered to day-4- chick embryo in ovo, micromelia was obviously observed in the hind limbs of 7-day chick embryos. We examined the teratogenic mechanism of 6-AN by using the normal or micromelial hind limbs (buds) from day 5 to day 7, with special at...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a133890

    authors: Honda A,Iwama M,Umeda T,Mori Y

    更新日期:1982-06-01 00:00:00

  • Epithelial-mesenchymal transition in haematopoietic stem cell development and homeostasis.

    abstract::Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审

    doi:10.1093/jb/mvy063

    authors: Hamidi S,Sheng G

    更新日期:2018-10-01 00:00:00

  • Signalling pathway involving GULP, MAPK and Rac1 for SR-BI-induced phagocytosis of apoptotic cells.

    abstract::Class B scavenger receptor type I (SR-BI) is a phosphatidylserine (PS)-recognizing receptor of testicular Sertoli cells responsible for the phagocytosis of spermatogenic cells undergoing apoptosis. Here, we determined signal mediators that compose a signalling pathway for SR-BI-induced phagocytosis. Results of a yeast...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvn176

    authors: Osada Y,Sunatani T,Kim IS,Nakanishi Y,Shiratsuchi A

    更新日期:2009-03-01 00:00:00

  • Identification of essential residues of CTLA-2alpha for inhibitory potency.

    abstract::To identify functionally essential sequences and residues of CTLA-2alpha, in vitro mutagenesis was carried out. The coefficient of inhibition (K(i)) was determined towards rabbit cathepsin L using Z-Phe-Arg-MCA as the substrate. Recombinant CTLA-2alpha inhibited the enzyme potently (K(i) = 15 nM). A truncated mutant, ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/jb/mvp188

    authors: Deshapriya RM,Yuhashi S,Usui M,Kageyama T,Yamamoto Y

    更新日期:2010-03-01 00:00:00

  • Presence of two transcribed malate synthase genes in an n-alkane-utilizing yeast, Candida tropicalis.

    abstract::The presence of two genomic DNA regions encoding malate synthase (MS) was shown by Southern blot analysis of the genomic DNA from an n-alkane-assimilating yeast, Candida tropicalis, using a partial MS cDNA probe, in accordance with the fact that two types of partial MS cDNAs have previously been isolated. This was als...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.jbchem.a123688

    authors: Hikida M,Atomi H,Fukuda Y,Aoki A,Hishida T,Teranishi Y,Ueda M,Tanaka A

    更新日期:1991-12-01 00:00:00