Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility associated S6-RNase in Nicotiana alata.

abstract：:The scaffolding protein Salvador (Sav) plays a key role in the Hippo (Hpo) signalling pathway, which controls tissue growth by inhibiting cell proliferation and promoting apoptosis. Dysregulation of the Hippo pathway contributes to cancer development. Since the identification of the first Sav gene in 2002, very little...

journal_title：Journal of biochemistry

authors： Song J,Hong HR,Yamashita E,Park IY,Lee SJ

更新日期：2015-09-01 00:00:00

abstract：:G-actin freed from exogenous ATP was added to the pieces of isolated acrosomal actin bundles from horseshoe crab sperm to form filaments as reported earlier (Tilney, L.G., Bonder, E.M., & DeRosier, D.J. (1981) J. Cell Biol. 90, 485-494). The growth of a filament was far more rapid at one end (the preferred end) than t...

journal_title：Journal of biochemistry

authors： Mabuchi I

更新日期：1983-10-01 00:00:00

abstract：:The binding properties of dynein arms to the A- and B-tubules of outer doublets of cilia from Tetrahymena pyriformis were examined, with the following results: 1. When 30s dynein purified from Tetrahymena cilia was added to doublets deficient in dynein arms, it bound to both A- and B-tubules almost equally and formed ...

journal_title：Journal of biochemistry

authors： Takahashi M,Tonomura Y

更新日期：1978-12-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:One of the 2 main hemoglobins of the sea snake Pelamis platurus, (the Yellow-bellied sea snake) comprising about 70% of the total hemoglobin, was separated by DEAE-Sephadex column chromatography. From results of gel filtration and iron content determination, both intact sea snake hemoglobin, and the isolated major hem...

journal_title：Journal of biochemistry

authors： Liu CS

更新日期：1975-07-01 00:00:00

abstract：:Aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (QPO) that catalyzes peroxidase activity using quinol in the respiratory chain. In the previous work, we have characterized recombinant QPO purified from t...

journal_title：Journal of biochemistry

authors： Abe T,Kawarai T,Takahashi Y,Konishi K

更新日期：2017-06-01 00:00:00

abstract：:Reaction center of chromatophores of Rhodospirillum rubrum consists of three kinds of protein, H-, M-, and L-subunit, and is bound with many other kinds of protein to form a larger protein complex (PRU; photoreaction unit), which contains all the bacteriochlorophyll. In the present study, purified PRU was dissociated ...

journal_title：Journal of biochemistry

authors： Tanaka K,Kakuno T,Yamashita J,Horio T

更新日期：1983-12-01 00:00:00

abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

journal_title：Journal of biochemistry

authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1993-06-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:Telomerase is an enzyme that catalyzes addition of telomeric repeat sequences to the 3'-termini of eukaryotic chromosome DNA. The catalytic core of telomerase consists of a protein component, telomerase reverse transcriptase (TERT), for the catalysis and an RNA component, telomerase RNA (TR), containing the template f...

journal_title：Journal of biochemistry

authors： Li X,Nishizuka H,Tsutsumi K,Imai Y,Kurihara Y,Uesugi S

更新日期：2007-05-01 00:00:00

abstract：:The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

journal_title：Journal of biochemistry

authors： Takenaka H,Ikehara M,Tonomura Y

更新日期：1976-12-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:Chorismate mutase of Brevibacterium flavum, a common enzyme in phenylalanine and tyrosine biosynthesis, was separted into two different component, A and B, with molecular weights of 250,000 and 25,000, respectively, by ammonium sulfate fractionation or gel-filtration. Both components were essential for the enzymatic a...

journal_title：Journal of biochemistry

authors： Shiio I,Sugimoto S

更新日期：1979-07-01 00:00:00

abstract：:Galactocerebrosidase was purified about 22,600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 x 10(5) nmol/h/mg with tritium-labeled galactocerebroside as the substrate in the taurocholate system. The optimal pH for galactocer...

journal_title：Journal of biochemistry

authors： Sakai N,Inui K,Midorikawa M,Okuno Y,Ueda S,Iwamatsu A,Okada S

更新日期：1994-09-01 00:00:00

abstract：:It has been reported that two kinds of Cd-binding peptide (Cd-BP1 and Cd-BP2) are induced in fission yeast upon exposure to Cd, and that they consist of the same unit peptide (cadystin), but Cd-BP1 binds 1.5 times more Cd atoms per cadystin than Cd-BP2 (Murasugi, A., Wada, C., & Hayashi, Y. (1981) J. Biochem. 90, 1561...

journal_title：Journal of biochemistry

authors： Murasugi A,Wada Nakagawa C,Hayashi Y

更新日期：1984-11-01 00:00:00

abstract：:Mitochondria are dynamic organelles that frequently divide and fuse together, resulting in the formation of intracellular tubular networks. In yeast and mammals, several factors including Drp1/Dnm1 and Mfn/Fzo1 are known to regulate mitochondrial morphology by controlling membrane fission or fusion. Here, we report th...

journal_title：Journal of biochemistry

authors： Ichishita R,Tanaka K,Sugiura Y,Sayano T,Mihara K,Oka T

更新日期：2008-04-01 00:00:00

abstract：:Plasmapheresis with a dextran sulfate column is a treatment for patients with hypercholesteremia. When proteins bound to the column during the treatment were fractionated to prepare some known proteins, we found a 57 kDa glycoprotein designated GP57 which showed a new N-terminal amino acid sequence. Western-blot analy...

journal_title：Journal of biochemistry

authors： Choi-Miura NH,Sano Y,Oda E,Nakano Y,Tobe T,Yanagishita T,Taniyama M,Katagiri T,Tomita M

更新日期：1995-02-01 00:00:00

abstract：:The promoter -10 binding region of the Bacillus subtilis sigma(A) factor forms an amphiphilic alpha-helix with three conserved isoleucines located at four-residue intervals. To investigate the structural and functional roles of the three isoleucine residues, we constructed a series of sigA mutants with single and doub...

journal_title：Journal of biochemistry

authors： Liao CT,Wang WH,Yang HS,Chen JP,Chang BY

更新日期：1999-09-01 00:00:00

abstract：:Histidinol dehydrogenase (HDH), a Zn-metalloenzyme, produces His from histidinol through two successive oxidation reactions with NAD+ as a coenzyme. A mutation, His261-->Asn, caused the complete loss of the Zn, thereby inactivating the enzyme, without significant structural perturbation. The ability to oxidize an inte...

journal_title：Journal of biochemistry

authors： Nagai A,Ohta D

更新日期：1994-01-01 00:00:00

abstract：:cAMP and Ca2(+)-independent histone kinase was generated from rat liver plasma membrane in an ionic strength-dependent manner by the action of an endogenous trypsin-like protease (Hashimoto, E. et al. (1986) FEBS Lett. 200, 63-66). In addition to the effect of ionic strength, this proteolytic activation of protein kin...

journal_title：Journal of biochemistry

authors： Hashimoto E,Yamamura H

更新日期：1989-12-01 00:00:00

abstract：:We generated and characterized novel antibodies specific for a cleavage site of human caspase-8/FLICE and its substrate, FLICE-like inhibitory protein (FLIP). The synthetic peptides used as immunogens were CQGDNYQKGIPVETD (#791) and VSEGQLEDSSLLEVD (#1342), which corresponded to cleaved regions of N-terminal fragments...

journal_title：Journal of biochemistry

authors： Niikura Y,Nonaka T,Imajoh-Ohmi S

更新日期：2002-07-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:To identify functionally essential sequences and residues of CTLA-2alpha, in vitro mutagenesis was carried out. The coefficient of inhibition (K(i)) was determined towards rabbit cathepsin L using Z-Phe-Arg-MCA as the substrate. Recombinant CTLA-2alpha inhibited the enzyme potently (K(i) = 15 nM). A truncated mutant, ...

journal_title：Journal of biochemistry

authors： Deshapriya RM,Yuhashi S,Usui M,Kageyama T,Yamamoto Y

更新日期：2010-03-01 00:00:00

abstract：:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we c...

journal_title：Journal of biochemistry

authors： Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang M

更新日期：2019-09-01 00:00:00

abstract：:The transcription and replication of influenza RNA can be studied in vitro by the reconstitution of functional ribonucleoprotein (RNP) complex from viral core proteins including the RNA polymerase (complex of three P protein subunits) and nucleoprotein (NP), and model templates. Here, two different core protein prepar...

journal_title：Journal of biochemistry

authors： Seong BL,Kobayashi M,Nagata K,Brownlee GG,Ishihama A

更新日期：1992-04-01 00:00:00

abstract：:Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized...

journal_title：Journal of biochemistry

authors： Kitagawa H,Nakada H,Fukui S,Funakoshi I,Kawasaki T,Yamashina I,Tate S,Inagaki F

更新日期：1993-10-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain ...

journal_title：Journal of biochemistry

authors： Amaya Y,Nakai T,Miura S

更新日期：2016-04-01 00:00:00