Purification and characterization of a novel glycoprotein which has significant homology to heavy chains of inter-alpha-trypsin inhibitor family from human plasma.

abstract：:We synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. We solubilized membrane proteins of Vibrio parahaemolyticus using the detergents. With octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfacto...

journal_title：Journal of biochemistry

authors： Izawa S,Sakai-Tomita Y,Kinomura K,Kitazawa S,Tsuda M,Tsuchiya T

更新日期：1993-05-01 00:00:00

abstract：:Ribose-modified fluorescent nucleotide analogs, 3'-O-anthraniloyl and 3'-O-(N-methylanthraniloyl) derivatives of AT(D)P, dAT(D)P, CT(D)P, UT(D)P, IT(D)P, and GT(D)P, were synthesized for use as substrates and affinity labels for the myosin ATPase [Hiratsuka, T. (1983) Biochim. Biophys. Acta 742, 496-508]. None of the ...

journal_title：Journal of biochemistry

authors： Hiratsuka T

更新日期：1984-07-01 00:00:00

abstract：:Formate dehydrogenase (EC 1.2.1.2) prepared from peas (Pisum sativum) was a two-subunit enzyme. The enzyme accelerated the formation of an NAD+-cyanide compound having an adsorption band at 330 nm. The enzyme was able to bind one NAD+ molecule per each subunit but only 1 mole of NAD+-cyanide compound was formed per ...

journal_title：Journal of biochemistry

authors： Ohyama T,Yamazaki I

更新日期：1975-04-01 00:00:00

abstract：:Tn-antigen (GalNAcα-Ser/Thr) is one of the most common aberrations associated with cancer progression and metastasis, and thus is an excellent target for development of cancer diagnostics and therapeutics. MLS128 monoclonal antibody (mAb), derived from a mouse immunized with human colon carcinoma cells, was reported t...

journal_title：Journal of biochemistry

authors： Fujita-Yamaguchi Y

更新日期：2013-08-01 00:00:00

abstract：:A cell-free protein-synthesizing system active in initiation of translation of both endogenous mRNA and exogenous mRNA has been obtained from postmitochondrial supernatant (S-12) of the liver of ethionine-treated rats by adding reticulocyte ribosomal extract as a source of initiation factor. Formation of polysomes in ...

journal_title：Journal of biochemistry

authors： Hase M,Endo Y,Natori Y

更新日期：1982-05-01 00:00:00

abstract：:Thyrotropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the TRH-induced hydrolysis of PIP2, the conversion of phosphatidylinositol 4-phosphate (PIP) to PIP2 and the accumulation...

journal_title：Journal of biochemistry

authors： Suzuki R,Takenawa T

更新日期：1987-09-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:The cDNAs encoding the precursors of cystatin SN, cystatin S, and two mutants of cystatin S (-18R-->W; 117R-->W) were expressed in Escherichia coli JM109 with isopropyl-beta-D-thio-galactoside (IPTG) induction. Premature cystatin S with the original signal [-20MARPLCTLLLLMATLAGALA] was processed and a large amount of ...

journal_title：Journal of biochemistry

authors： Saitoh E,Isemura S

更新日期：1994-08-01 00:00:00

abstract：:Paramyosin is a myosin-binding protein characteristic of invertebrate animals, while troponin is a Ca2+-dependent regulator of muscle contraction. Both proteins are widely distributed in protostomes, while in deuterostomes, their distribution is limited; namely, presence of paramyosin and absence of troponin are commo...

journal_title：Journal of biochemistry

authors： Sonobe H,Obinata T,Minokawa T,Haruta T,Kawamura Y,Wakatsuki S,Sato N

更新日期：2016-12-01 00:00:00

abstract：:An artificial gene coding for the human cap binding protein (hCBP: human IF-4E) was chemically synthesized and expressed in Escherichia coli under the control of a trp promoter. The DNA duplex of 662 bp was designed and constructed from 44 oligodeoxynucleotide fragments of typically 30 nucleotides in length. Although ...

journal_title：Journal of biochemistry

authors： Ueda H,Maruyama H,Doi M,Inoue M,Ishida T,Morioka H,Tanaka T,Nishikawa S,Uesugi S

更新日期：1991-06-01 00:00:00

abstract：:Heptic fibrosis/cirrhosis is a common hepatic disease characterized by the hyper-accumulation of connective tissue components, and hepatic necrosis. Chronic alcohol ingestion, viral infection, and metabolic disorders are contributing factors and there has been no effective treatment. Hepatocyte growth factor (HGF), or...

journal_title：Journal of biochemistry

authors： Matsuda Y,Matsumoto K,Ichida T,Nakamura T

更新日期：1995-09-01 00:00:00

abstract：:Gram-positive thermophilic Bacillus species contain cytochrome caa3-type cytochrome c oxidase as their main terminal oxidase in the respiratory chain. To identify alternative oxidases, we isolated several mutants from B. stearothermophilus defective in the caa3-type oxidase activity [Sakamoto, J. et al (1996) FEMS Mic...

journal_title：Journal of biochemistry

authors： Sakamoto J,Handa Y,Sone N

更新日期：1997-10-01 00:00:00

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:A new arginine derivative, N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide hydrochloride (ZPVAPA.HCl) was synthesized by the condensation of N-benzyloxy-carbonyl-L-phenylalanyl-L-valine and L-arginine-p-nitroanilide dihydrochloride using dicyclohexylcarbodiimide as a coupling reagent and 1-hydroxy...

journal_title：Journal of biochemistry

authors： Somorin O,Tokura S,Nishi N,Noguchi J

更新日期：1979-01-01 00:00:00

abstract：:In order to characterize low affinity ATP-binding sites of renal (Na+,K+) ATPase and sarcoplasmic reticulum (Ca2+)ATPase, the effects of ATP on the splitting of the K+-sensitive phosphoenzymes were compared. ATP inactivated the dephosphorylation in the case of (Na+,K+)ATPase at relatively high concentrations, while ac...

journal_title：Journal of biochemistry

authors： Fukushima Y,Yamada S,Nakao M

更新日期：1984-02-01 00:00:00

abstract：:Vertebrate skeletal muscle alpha-tropomyosin polymerizes in a head-to-tail manner and binds cooperatively to actin. It has been postulated that the cooperative actin binding is governed by the strength of the head-to-tail interaction. In order to know the relationship between the head-to-tail affinity and actin bindin...

journal_title：Journal of biochemistry

authors： Sano K,Maeda K,Oda T,Maéda Y

更新日期：2000-06-01 00:00:00

abstract：:A contractile protein closely resembling natural actomyosin (myosin B) of rabbit skeletal muscle was extracted from plasmodia of the slime mold, Physarum polycephalum, by protecting the SH-groups with beta-mercaptoethanol or dithiothreitol. Superprecipitation of the protein induced by Mg2+-ATP at low ionic strength wa...

journal_title：Journal of biochemistry

authors： Kato T,Tonomura Y

更新日期：1975-06-01 00:00:00

abstract：:We reported previously competitive panning elution with PBS (pH 7.0) that contains HM-1 killer toxin (HM-1) and Candida albicans membrane fraction (CaMF) to release phages bound with CaMF as an antigen. Here, as an alternative strategy, we isolated high-binding affinity recombinant single-chain fragment variables (scF...

journal_title：Journal of biochemistry

authors： Krishnaswamy S,Kabir ME,Miyamoto M,Furuichi Y,Komiyama T

更新日期：2010-05-01 00:00:00

abstract：:We evaluated the role of cytosolic phospholipase A2 (PLA2) in the exocytosis of amylase from parotid acinar cells. The exocytosis stimulated by isoproterenol was dose-dependently inhibited by bromoenol lactone (BEL), a potent suicide inhibitor of Ca2+-independent cytosolic PLA2. The IC50 value of BEL was approximately...

journal_title：Journal of biochemistry

authors： Takuma T,Ichida T

更新日期：1997-06-01 00:00:00

abstract：:A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

journal_title：Journal of biochemistry

authors： Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

更新日期：1983-12-01 00:00:00

abstract：:N-acylethanolamines (NAEs) are a class of bioactive lipid molecules in animal tissues, including the endocannabinoid anandamide and the anti-inflammatory substance N-palmitoylethanolamine. Enzymatic hydrolysis of NAEs is considered to be an important step to regulate their endogenous levels. Lysosomal NAE-hydrolysing ...

journal_title：Journal of biochemistry

authors： Wang J,Zhao LY,Uyama T,Tsuboi K,Wu XX,Kakehi Y,Ueda N

更新日期：2008-11-01 00:00:00

abstract：:Administration of an excessive dose of Bacillus subtilis alpha-amylase [EC 3.2.1.1, alpha-1,4-glucan 4-glucanohydrolase] (BalphaA) induced the production of non-precipitating (non-ppt) IgG2 antibody in guinea pigs, whereas immunization with a normal dose produced precipitating (ppt) IgG1 and IgG2 antibodies. The non-p...

journal_title：Journal of biochemistry

authors： Mori N,Nakamura T,Koyama J

更新日期：1977-07-01 00:00:00

abstract：:OsCnfU-1A is a chloroplast-type Nfu-like protein that consists of tandem repeats sharing high sequence homology. Domain I of this protein, but not domain II, has a C-X-X-C motif that is thought to assemble an iron-sulphur cluster. Herein we report the solution structure of OsCnfU-1A domain I (73-153). Although OsCnfU-...

journal_title：Journal of biochemistry

authors： Saio T,Kumeta H,Ogura K,Yokochi M,Asayama M,Katoh S,Katoh E,Teshima K,Inagaki F

更新日期：2007-07-01 00:00:00

abstract：:Four motor proteins, MotX, MotY, PomA, and PomB, have been identified as constituents of the Na(+)-driven flagellum of Vibrio species. In this study, the complete motX gene was cloned from Vibrio alginolyticus and shown to complement three mot mutations, motX94, motX115, and motX119, as well as a V. parahaemolyticus m...

journal_title：Journal of biochemistry

authors： Okabe M,Yakushi T,Asai Y,Homma M

更新日期：2001-12-01 00:00:00

abstract：:The minimal structural unit of cytochrome c oxidase purified from Thiobacillus novellus was composed of one molecule each of two subunits with molecular masses of 32 and 23 kDa, respectively, and the unit had one molecule of heme a and one atom of copper. In the presence of n-octyl-beta-D-thioglucoside, the oxidase ex...

journal_title：Journal of biochemistry

authors： Shoji K,Yamazaki T,Nagano T,Fukumori Y,Yamanaka T

更新日期：1992-01-01 00:00:00

abstract：:Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombri...

journal_title：Journal of biochemistry

authors： Okamoto Y,Ogawa K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1993-06-01 00:00:00

abstract：:The conditions for the preparation of the ferricytochrome a-ferrocytochrome a3-carbon monoxide complex (a3+, a3(2)+CO) of cytochrome oxidase [EC 1.9.3.1] by the ferricyanide-reoxidation method and some properties of the prepared complex were studied. The addition of a small volume of concentrated ferricyanide solution...

journal_title：Journal of biochemistry

authors： Horie S,Watanabe T,Ave K

更新日期：1983-04-01 00:00:00

abstract：:Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported...

journal_title：Journal of biochemistry

authors： Kashima N,Matsui H,Yamasaki S,Kondo N,Hamuro J

更新日期：1987-10-01 00:00:00

abstract：:In the nucleus of eukaryotic cells, chromatin is tethered to the nuclear envelope (NE), wherein inner nuclear membrane proteins (INMPs) play major roles. However, in Xenopus blastula, chromatin tethering to the NE depends on nuclear filamentous actin that develops in a blastula-specific manner. To investigate whether ...

journal_title：Journal of biochemistry

authors： Oda H,Kato S,Ohsumi K,Iwabuchi M

更新日期：2020-11-10 00:00:00

abstract：:Calf thymus DNA polymerases alpha and beta [EC 2.7.7.7] and terminal deoxynucleotidyl transferase [EC 2.7.7.31] were analyzed on two-dimensional gel slabs. DNA polymerase beta appeared as a single spot on two-dimensional gel at the position of 40,000 daltons and pI 8.0 using non-equilibrium pH gradient gel electrophor...

journal_title：Journal of biochemistry

authors： Koiwai O,Masaki S,Kaneda T,Yoshida S

更新日期：1982-08-01 00:00:00