Abstract:
:A new arginine derivative, N-benzyloxycarbonyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide hydrochloride (ZPVAPA.HCl) was synthesized by the condensation of N-benzyloxy-carbonyl-L-phenylalanyl-L-valine and L-arginine-p-nitroanilide dihydrochloride using dicyclohexylcarbodiimide as a coupling reagent and 1-hydroxy-benzotriazole as an additive. L-ZPVAPA.HCl was split by trypsin more readily than Na-benzyloxycarbonyl-L-arginine-p-nitroanilide hydrochloride (L-ZAPA, HCl), Na-benzoyl-L-arginine-p-nitroanilide hydrochloride (L-BAPA.HCl), Na-tosyl-L-arginine-p-nitroanilide hdyrochloride (L-TAPA.HCl) and Na-benzoyl-DL-arginine-p-nitroanilide hydrochloride (DL-BAPA.HCl) by factors of 100, 400, 600, and 1,200, respectively. Low concentrations of dimethyl formamide (DMF) enhanced the trypsin-catalyzed hydrolyses of L-ZAPA.HCl and L-TAPA.HCl, contrary to the findings of other authors that DMF has no effect on the tryptic hydrolysis.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Somorin O,Tokura S,Nishi N,Noguchi Jdoi
10.1093/oxfordjournals.jbchem.a132305subject
Has Abstractpub_date
1979-01-01 00:00:00pages
157-62issue
1eissn
0021-924Xissn
1756-2651journal_volume
85pub_type
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更新日期:1981-08-01 00:00:00
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更新日期:1991-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135062
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pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
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更新日期:1988-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123901
更新日期:1992-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133620
更新日期:1981-11-01 00:00:00
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更新日期:2002-06-01 00:00:00
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更新日期:2009-01-01 00:00:00