Purification and properties of rat D-3-hydroxyacyl-CoA dehydratase: D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein.

abstract：:The structure of a ferricytochrome c' extracted from Rhodospirillum rubrum has been determined at 6 A resolution by the X-ray crystallographic method. The crystals, obtained by dialyzing the protein solution against polyethylene glycol 4000, belong to the hexagonal space group P6(1). Two heavy atom derivatives were ob...

journal_title：Journal of biochemistry

authors： Yasui M,Harada S,Kai Y,Kasai N

更新日期：1985-07-01 00:00:00

abstract：:Centromere protein B (CENP-B) is one of the centromere DNA binding proteins constituting centromeric heterochromatin of human chromosomes. This protein was originally identified as the target antigen in autoimmune disease patients (often with scleroderma). In this study, we cloned a human CENP-B cDNA which was longer ...

journal_title：Journal of biochemistry

authors： Sugimoto K,Muro Y,Himeno M

更新日期：1992-04-01 00:00:00

abstract：:To analyze the processing of extracellular enzymes of Bacillus subtilis, an NH2-terminally extended hybrid alpha-amylase [pTUBE638-alpha-amylase (E24)] was purified from the periplasm of E. coli(pTUBE638) as the substrate for the in vitro processing reaction, in which a 21-amino-acid extra-peptide was added at the NH2...

journal_title：Journal of biochemistry

authors： Itoh Y,Sumi M,Nakamura K,Yamane K

更新日期：1990-12-01 00:00:00

abstract：:A cell-wall-associated proteinase from Lactobacillus helveticus CP790, grown in skim milk, was purified to homogeneity by DEAE ion exchange chromatography in the presence of EDTA. Its molecular weight was estimated to be 45,000 by SDS-PAGE and also by the recovery of proteinase activity only from this fraction of SDS-...

journal_title：Journal of biochemistry

authors： Yamamoto N,Akino A,Takano T

更新日期：1993-11-01 00:00:00

abstract：:The pH dependence of the binding constant of Ca2+ to a phospholipase A2 of Agkistrodon halys blomhoffii, in which the alpha-amino group had been selectively modified to an alpha-keto group, was studied at 25 degrees C and ionic strength 0.1 by the tryptophyl fluorescence method. The dependence was compared with the re...

journal_title：Journal of biochemistry

authors： Haruki H,Teshima K,Samejima Y,Kawauchi S,Ikeda K

更新日期：1986-01-01 00:00:00

abstract：:The peptide bond syntheses catalyzed by subtilisin BPN', papain [EC 3.4.22.2], and pepsin [EC 3.4.23.1] were studied comparatively at the optimum pH of the enzymes with the coupling system Cbz-(AA)n-OH + Leu-X leads to Cbz-(AA)n-Leu-X, in which AA = various amino acid residues, n = 1-3 and X = -NH2, -OEt, -OBut, -ODPM...

journal_title：Journal of biochemistry

authors： Morihara K,Oka T

更新日期：1981-02-01 00:00:00

abstract：:Cholesterol: oxygen oxidoreductase [EC 1.1.3.6] from Brevibacterium sterolicum (ATCC 21387) was found to catalyze the oxidation of steroids such as sterols, steroid hormones, and bile acids having a free C-3beta hydroxyl group. However, the enzyme was inactive towards estradiol and estriol and had a weak activity towa...

journal_title：Journal of biochemistry

authors： Ikawa S,Takita M,Ogura M

更新日期：1979-06-01 00:00:00

abstract：:The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis tra...

journal_title：Journal of biochemistry

authors： Itaya M,Nagata T,Shiroishi T,Fujita K,Tsuge K

更新日期：2000-11-01 00:00:00

abstract：:Microtubule-associated protein (MAP) 1 consisting of MAP 1A and 1B was purified from rat brain by the poly-L-aspartic acid (PLAA) method. We found that MAP 1 bound to F-actin in vitro up to a molar ratio of MAP 1 to actin monomers of 1:10. The apparent binding constant was about 2.7 x 10(7) M-1. In contrast to the bin...

journal_title：Journal of biochemistry

authors： Fujii T,Watanabe M,Ogoma Y,Kondo Y,Arai T

更新日期：1993-12-01 00:00:00

abstract：:The primary structure of human IGF-I, except for the disulfide bond system, has been reported by Rinderknecht and Humbel. IGF-I afforded the corresponding characteristic peptide fragment on V8 protease digestion, which contained Cys6, Cys47, Cys48, and Cys52. Two possible fragments, Type I with Cys6-Cys47 and Cys48-Cy...

journal_title：Journal of biochemistry

authors： Iwai M,Kobayashi M,Tamura K,Ishii Y,Yamada H,Niwa M

更新日期：1989-12-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:Three isozymic forms, alpha, beta, and gamma, of Drosophila melanogaster aldolase are produced from a single gene by alternative usage of the triple exons 4 (4 alpha, 4 beta, and 4 gamma) [Shaw-Lee et al. (1992) J. Biol. Chem. 267, 3959-3967; Kim et al. (1992) Mol. Cell. Biol. 12, 773-783; Kai et al. (1992) J. Biochem...

journal_title：Journal of biochemistry

authors： Zhang R,Kai T,Sugimoto Y,Kusakabe T,Takasaki Y,Koga K,Hori K

更新日期：1995-07-01 00:00:00

abstract：:We have examined critically whether or not new and old histones mix in the octameric units of nucleosomes during chromatin replication. MH-134SC cells were density-labeled by culturing with amino acid mixtures enriched with dense isotopes 2H, 13C, and 15N. Mononucleosomes obtained from labeled cells were fractionated ...

journal_title：Journal of biochemistry

authors： Yamasu K,Senshu T

更新日期：1987-04-01 00:00:00

abstract：:Monoclonal antibody 5E5 recognized an intranuclear antigen of neurons in the rat. We isolated 5E5cDNA and determined its nucleotide and deduced amino acid sequences. The 5E5cDNA had an open reading frame of 825 amino acids and its amino acid sequence showed no significant homology to any protein or to any DNA binding ...

journal_title：Journal of biochemistry

authors： Suzuki E,Kojima N,Yoshimura K,Uyemura K,Obata K,Akagawa K

更新日期：1995-07-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00

abstract：:RPE65 is a membrane-associated retinoid isomerase involved in the visual cycle responsible for sustaining vision. Many mutations in the human RPE65 gene are associated with distinct forms of retinal degenerative diseases. The pathogenic mechanisms for most of these mutations remain poorly understood. Here, we show tha...

journal_title：Journal of biochemistry

authors： Li S,Hu J,Jin RJ,Aiyar A,Jacobson SG,Bok D,Jin M

更新日期：2015-08-01 00:00:00

abstract：:A fluorescent fragment of Mr = 23,800 was obtained by the papain digestion of N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene diamine (abbreviated as IAEDANS)-modified chicken gizzard myosin. The fragment was isolated by gel filtration on a Sephadex G-100 column in the presence of 5 M guanidine-HCl followed by anion exch...

journal_title：Journal of biochemistry

authors： Onishi H,Maita T,Miyanishi T,Watanabe S,Matsuda G

更新日期：1986-12-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:Actin-activated ATPase activity of myosin from Physarum polycephalum decreases when it binds Ca2+ and increases when it loses Ca2+. This Ca-inhibition is observed with phosphorylated myosin [Kohama, K. (1990) Trend, Pharmacol. Sci. 11, 433-435]. The activity of dephosphorylated myosin remained at a low level both in t...

journal_title：Journal of biochemistry

authors： Kohama K,Kohno T,Okagaki T,Shimmen T

更新日期：1991-10-01 00:00:00

abstract：:The binding of actin filaments to connectin, a muscle elastic protein, was investigated by means of turbidity and sedimentation measurements and electron microscopy. In the presence of less than 0.12 M KCl at pH 7.0, actin filaments bound to connectin. Long actin filaments formed bundles. Short actin filaments also ag...

journal_title：Journal of biochemistry

authors： Maruyama K,Hu DH,Suzuki T,Kimura S

更新日期：1987-06-01 00:00:00

abstract：:Rous sarcoma virus-transformed rat liver cell line RSV-BRL secreted a neutral proteinase in a latent precursor form with a molecular weight (Mr) of 57,000 (57k) as a major secreted protein. This enzyme was a calcium-dependent metallo-proteinase. The proenzyme was purified from the serum-free conditioned medium of the ...

journal_title：Journal of biochemistry

authors： Umenishi F,Yasumitsu H,Ashida Y,Yamauti J,Umeda M,Miyazaki K

更新日期：1990-10-01 00:00:00

abstract：:Matriptase is a type II transmembrane serine protease expressed abundantly in the epithelial cells and keratinocyte. It plays a key role in the establishment and maintenance of epithelial integrity. Matriptase is considered to be at the most upstream in cellular protease cascade. Activation of its zymogen is the most ...

journal_title：Journal of biochemistry

authors： Kojima K,Inouye K

更新日期：2011-08-01 00:00:00

abstract：:The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1,557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (Mr 55,830). The predicted amino acid sequence was consistent with the partial a...

journal_title：Journal of biochemistry

authors： Xu XM,Koyama N,Cui M,Yamagishi A,Nosoh Y,Oshima T

更新日期：1991-05-01 00:00:00

abstract：:Ornithine transcarbamylase [EC 2.1.3.3] has been purified from chick kidney to homogeneity. The molecular weight is 110,000 as determined by gel filtration. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the enzyme showed that the enzyme exists as a trimer of identical subunits of 36,000 daltons like othe...

journal_title：Journal of biochemistry

authors： Tsuji S

更新日期：1983-10-01 00:00:00

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:In the preceding papers, we showed that one of the two complementar factors of polypeptide chain elongation factor 1 (EF-1) from pig liver, EF-1alpha, functionally corresponds to bacterial EF-Tu (Nagata, S., Iwasaki, K., and Kaziro, Y. (1976) Arch. Biochem. Biophys. 172, 168), while the other, EF-1betagamma, as well a...

journal_title：Journal of biochemistry

authors： Nagata S,Motoyoshi K,Iwasaki K

更新日期：1978-02-01 00:00:00

abstract：:Resonance Raman (RR) spectra were obtained in H2O or D2O solution for the purple intermediates of D-amino acid oxidase (DAO) with isotopically labeled substrates, i.e., [1-13C]-, [2-13C]-, [3-13C]-, [15N]-, and [3,3,3-D3]alanine; [carboxyl-13C]- and [15N]proline. RR spectra were also measured for the intermediates of ...

journal_title：Journal of biochemistry

authors： Nishina Y,Shiga K,Miura R,Tojo H,Ohta M,Miyake Y,Yamano T,Watari H

更新日期：1983-12-01 00:00:00

abstract：:The modes of actions of 1-farnesylpyridinium (FPy) on yeast cell growth were investigated on the basis of its effects on cell cycle progression, morphogenesis and the related events for construction of cell wall architecture in Schizosacchromyces pombe. FPy predominantly inhibited the growth of the yeast cells after v...

journal_title：Journal of biochemistry

authors： Hamada M,Ohata I,Fujita K,Usuki Y,Ogita A,Ishiguro J,Tanaka T

更新日期：2006-12-01 00:00:00

abstract：:Glycosynthases are engineered glycoside hydrolases (GHs) that catalyse the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors. We have determined five crystal structures of the glycosynthase mutants reducing-end xylose-releasing exo-oligoxylanase, an inverting GH, that exhibit various levels o...

journal_title：Journal of biochemistry

authors： Hidaka M,Fushinobu S,Honda Y,Wakagi T,Shoun H,Kitaoka M

更新日期：2010-02-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00