Drosophila melanogaster aldolase: characterization of the isozymes alpha, beta, and gamma generated from a single gene.

abstract：:It has been reported that two kinds of Cd-binding peptide (Cd-BP1 and Cd-BP2) are induced in fission yeast upon exposure to Cd, and that they consist of the same unit peptide (cadystin), but Cd-BP1 binds 1.5 times more Cd atoms per cadystin than Cd-BP2 (Murasugi, A., Wada, C., & Hayashi, Y. (1981) J. Biochem. 90, 1561...

journal_title：Journal of biochemistry

authors： Murasugi A,Wada Nakagawa C,Hayashi Y

更新日期：1984-11-01 00:00:00

abstract：:The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphoryla...

journal_title：Journal of biochemistry

authors： Miyazawa K

更新日期：2013-01-01 00:00:00

abstract：:Mitochondria have two independent protein-import machineries, one in the outer membrane (the Tom system) and the other in the inner membrane (the Tim system). Here, we have characterized the initial steps of precursor import into rat liver mitoplasts. The import reaction was separated into two stages, consisting of pr...

journal_title：Journal of biochemistry

authors： Ishihara N,Komiya T,Sakaguchi M,Ito A,Mihara K

更新日期：1998-10-01 00:00:00

abstract：:We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-tre...

journal_title：Journal of biochemistry

authors： Hoshi K,Kariya Y,Nara K,Ito H,Matsumoto K,Nagae M,Yamaguchi Y,Nakajima M,Miyajima M,Arai H,Kuno A,Narimatsu H,Shirotani K,Hashimoto Y

更新日期：2013-09-01 00:00:00

abstract：:To investigate the effect of plasminogen on cartilage catabolism, we assessed collagen degradation in rabbit articular cartilage explants treated with or without plasminogen and interleukin-1alpha (IL-1alpha). The combination of IL-1 alpha and plasminogen induced rapid collagen degradation, amounting to more than 60% ...

journal_title：Journal of biochemistry

authors： Saito S,Katoh M,Masumoto M,Matsumoto S,Masuho Y

更新日期：1997-07-01 00:00:00

abstract：:Heat-stable fructose 1,6-bisphosphate-dependent L-lactate dehydrogenase [EC 1.1.1.27] was purified from an extremely thermophilic bacterium, Thermus aquaticus YT-1. The amino acid composition and NH2-terminal 34 amino acid sequence of the enzyme were determined. Its NH2-terminal sequence shows high homology with those...

journal_title：Journal of biochemistry

authors： Machida M,Matsuzawa H,Ohta T

更新日期：1985-03-01 00:00:00

abstract：:Candida albicans is a commensal organism, but causes life-threatening infections in immunocompromised patients. Certain factors such as yeast-hyphae transition and hydrolytic enzymes are suggested as virulence attributes of C. albicans. Among them, 10 types of secreted aspartic protease (SAP) genes have received parti...

journal_title：Journal of biochemistry

authors： Aoki W,Kitahara N,Miura N,Morisaka H,Yamamoto Y,Kuroda K,Ueda M

更新日期：2011-10-01 00:00:00

abstract：:An acid proteinase different from pepsin was purified from bullfrog (Rana catesbeiana) gastric mucosa by chromatography on hydroxyapatite, Q-Sepharose, Con A-Sepharose 4B, and Mono Q columns. Its molecular weight after purification was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 45 kDa...

journal_title：Journal of biochemistry

authors： Inokuchi T,Kobayashi K,Horiuchi S

更新日期：1994-01-01 00:00:00

abstract：:To investigate the role of the C-terminal region of barley beta-amylase, plasmid delta 54 was constructed with an expression vector (pBETA92) of barley beta-amylase by site-directed mutagenesis. Escherichia coli JM109 harboring plasmid delta 54 was expected to express delta 54 beta-amylase in which 54 amino acid resid...

journal_title：Journal of biochemistry

authors： Yoshigi N,Sahara H,Koshino S

更新日期：1995-01-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:The flavoenzyme medium-chain acyl-CoA dehydrogenase (MCAD) eliminates the alpha-proton of the substrate analog, 3-thiaoctanoyl-CoA (3S-C8-CoA), to form a charge-transfer complex with deprotonated 3S-C8-CoA. This complex can simulate the metastable reaction intermediate immediately after the alpha-proton elimination of...

journal_title：Journal of biochemistry

authors： Satoh A,Nakajima Y,Miyahara I,Hirotsu K,Tanaka T,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Miura R

更新日期：2003-08-01 00:00:00

abstract：:Antiserum against Z-protein, which is one of the components of myofibrillar Z-lines, strongly stained the epithelial cells of chicken small intestine at the apical tips of the microvilli and the junctional complexes. Immunoblot tests with anti-Z-protein antiserum showed that there is a peptide of the same antigenicity...

journal_title：Journal of biochemistry

authors： Ohashi K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:Cilia and flagella of eukaryotic cells are evolutionarily conserved organelles with a microtubule-based axoneme as a scaffold. To fulfil their functions in cellular motility, sensory reception and developmental signalling, cilia contain unique proteins, such as receptors and ion channels. The assembly and maintenance ...

journal_title：Journal of biochemistry

authors： Nakayama K,Katoh Y

更新日期：2018-03-01 00:00:00

abstract：:Heat-stable L-lactate dehydrogenase [EC 1.1.1.27] was purified from an extremely thermophilic bacterium belonging to the genus Thermus, and it showed an allosteric nature dependent on fructose 1,6-bisphosphate as an effector. The enzyme had a molecular weight of approximately 120,000 with a subunit molecular weight of...

journal_title：Journal of biochemistry

authors： Taguchi H,Yamashita M,Matsuzawa H,Ohta T

更新日期：1982-04-01 00:00:00

abstract：:Photoaffinity labeling with [3H]cytochalasin B detects two D-glucose-sensitive proteins in the chicken embryo fibroblast (CEF) plasma membrane, which accumulate under conditions of glucose starvation and are probably involved in the glucose transport system (Pessin, J.E., et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, ...

journal_title：Journal of biochemistry

authors： Ishii T,Tillotson LG,Isselbacher KJ

更新日期：1986-01-01 00:00:00

abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

journal_title：Journal of biochemistry

authors： Takahashi K,Shimidzu M,Shindo H,Kawamoto T,Nishi M,Matsumoto U,Taniguchi S

更新日期：1987-05-01 00:00:00

abstract：:The sequence of the 5'-terminal 106 nucleotides of cucumber mosaic virus (strain Y) RNA 4, the mRNA coding for viral coat protein, has been determined. The first AUG was located at 77 nucleotides from the 5'-terminus and was confirmed to be an initiation codon by analysis of the N-terminal amino acid sequence of the p...

journal_title：Journal of biochemistry

authors： Hidaka S,Tsunasawa S,Yoon J,Narita K,Takanami Y,Kubo S,Miura K

更新日期：1985-01-01 00:00:00

abstract：:In order to stabilize human RNase 1 by introduction of an intramolecular cross-link, a mutant protein (4-118CL RNase 1), in which Arg4 and Val118 are replaced with cysteine residues and linked by a disulfide bond, was designed and expressed in Escherichia coli as inclusion bodies. The 4-118CL RNase 1 that refolded und...

journal_title：Journal of biochemistry

authors： Futami J,Tada H,Seno M,Ishikami S,Yamada H

更新日期：2000-08-01 00:00:00

abstract：:A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase ...

journal_title：Journal of biochemistry

authors： Takamura H,Kito M

更新日期：1991-03-01 00:00:00

abstract：:Previously, several workers reported that at very low ionic strength and in the presence of ATP, the extent of binding of S-1 with the F-actin-tropomyosin-troponin complex or regulated actin (FA-TM-TN) is unaffected by removal of Ca2+. However, in this study we found that during the ATPase reaction at physiological io...

journal_title：Journal of biochemistry

authors： Inoue A,Tonomura Y

更新日期：1982-04-01 00:00:00

abstract：:The mechanism of binding between thermolysin with its specific inhibitor, talopeptin (MKI), was studied kinetically with the stopped-flow method by monitoring the enhancement of tryptophan fluorescence caused by the complex formation. Only one relaxation obeying first-order kinetics was observed. The dependence of the...

journal_title：Journal of biochemistry

authors： Kitagishi K,Hiromi K

更新日期：1983-01-01 00:00:00

abstract：:A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the att...

journal_title：Journal of biochemistry

authors： Miyagawa A,Okuyama T

更新日期：1977-06-01 00:00:00

abstract：:Incubation of quiescent cultures of Swiss 3T3 cells with epidermal growth factor (EGF) caused an increase in c-myc mRNA. Under these conditions, EGF did not induce phosphoinositide turnover, formation of diacylglycerol, formation of inositol tris-, bis-, and monophosphates, protein kinase C activation, or Ca2+ mobiliz...

journal_title：Journal of biochemistry

authors： Tsuda T,Hamamori Y,Fukumoto Y,Kaibuchi K,Takai Y

更新日期：1986-12-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:The Mediator complex (Mediator) plays key roles in transcription and functions as the nexus for integration of various transcriptional signals. Previously, we screened for Mediator cyclin-dependent kinase (CDK)-interacting factors and identified three proteins related to chromatin regulation. One of them, SUZ12 is req...

journal_title：Journal of biochemistry

authors： Fukasawa R,Iida S,Tsutsui T,Hirose Y,Ohkuma Y

更新日期：2015-11-01 00:00:00

abstract：:Hemoglobin-vesicles (HbV) have been developed for use as artificial O(2) carriers in which a purified Hb solution is encapsulated within a phospholipid bilayer membrane. In this study, bovine Hb (BHb) was tested as a source of HbV instead of human Hb (HHb). We compared the preparation process and characteristics of BH...

journal_title：Journal of biochemistry

authors： Sakai H,Masada Y,Takeoka S,Tsuchida E

更新日期：2002-04-01 00:00:00

abstract：:Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

journal_title：Journal of biochemistry

authors： Seki-Chiba S,Ishimoto M

更新日期：1977-12-01 00:00:00

abstract：:Ornithine transcarbamylase [EC 2.1.3.3] has been purified from chick kidney to homogeneity. The molecular weight is 110,000 as determined by gel filtration. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the enzyme showed that the enzyme exists as a trimer of identical subunits of 36,000 daltons like othe...

journal_title：Journal of biochemistry

authors： Tsuji S

更新日期：1983-10-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00